Restriction map for
pFN21AB3434
(Circular) (Six-base) MAPSORT of: pFN21AB3434.seq Check: 8201 from: 1 to: 5674
pFN21AB3434 5674 bp
With 182 enzymes: SgfI *
October 20, 2009 10:46 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3484 278
Size: 53 83 186 936 1948 2468
Fragments arranged by size:
2468 1948 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3017 1937
Size: 1080 4594
Acc65I G'GTAC_C
Cuts at: 2679 2995 2679
Size: 316 5358
AclI AA'CG_TT
Cuts at: 1563 3801 4174 1563
Size: 2238 373 3063
Fragments arranged by size:
3063 2238 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2168 2558 3737 5121 5389 1632
Size: 536 390 1179 1384 268 1917
Fragments arranged by size:
1917 1384 1179 536 390 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5477
AflIII A'CryG_T
Cuts at: 1203 2394 4573 5550 1203
Size: 1191 2179 977 1327
Fragments arranged by size:
2179 1327 1191 977
AgeI A'CCGG_T
Cuts at: 4505 4505
Size: 5674
AhdI GACnn_n'nnGTC
Cuts at: 4403 4403
Size: 5674
AleI CACnn'nnGTG
Cuts at: 1166 2408 1166
Size: 1242 4432
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5674
AlwNI CAG_nnn'CTG
Cuts at: 2431 3417 4989 2431
Size: 986 1572 3116
Fragments arranged by size:
3116 1572 986
ApaLI G'TGCA_C
Cuts at: 2614 2729 3731 4887 2614
Size: 115 1002 1156 3401
Fragments arranged by size:
3401 1156 1002 115
ApoI r'AATT_y
Cuts at: 1493 2983 3209 3361 5470 1493
Size: 1490 226 152 2109 1697
Fragments arranged by size:
2109 1697 1490 226 152
AscI GG'CGCG_CC
Cuts at: 2740 2740
Size: 5674
AseI AT'TA_AT
Cuts at: 160 1032 4228 160
Size: 872 3196 1606
Fragments arranged by size:
3196 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5674
AvaI C'yCGr_G
Cuts at: 1870 1943 2070 2813 2882 2999 1870
Size: 73 127 743 69 117 4545
Fragments arranged by size:
4545 743 127 117 73 69
AvrII C'CTAG_G
Cuts at: 2167 2167
Size: 5674
BamHI G'GATC_C
Cuts at: 3004 3371 3004
Size: 367 5307
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2108 2679 2822 2995 4450
Size: 325 217 627 321 571 143 173 1455
Cuts at: 4450 618
Size: 1842
Fragments arranged by size:
1842 1455 627 571 325 321 217 173
143
BanII G_rGCy'C
Cuts at: 1391 2071 2820 2993 1391
Size: 680 749 173 4072
Fragments arranged by size:
4072 749 680 173
BbeI G_GCGC'C
Cuts at: 2826 2826
Size: 5674
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4703
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3865 3899 1903
Size: 34 50 34 1844 34 3678
Fragments arranged by size:
3678 1844 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3566 4776 3566
Size: 1210 4464
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5674
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2352 2719
Size: 107 122 71 631 605 337 343 367
Cuts at: 2719 4285 136
Size: 1566 1525
Fragments arranged by size:
1566 1525 631 605 367 343 337 122
107 71
BglII A'GATC_T
Cuts at: 5669 5669
Size: 5674
BlpI GC'TnA_GC
Cuts at: 2852 3091 2852
Size: 239 5435
Bme1580I G_kGCm'C
Cuts at: 1790 2113 2618 2733 3735 4891 1790
Size: 323 505 115 1002 1156 2573
Fragments arranged by size:
2573 1156 1002 505 323 115
BmgBI CAC'GTC
Cuts at: 1318 2383 1318
Size: 1065 4609
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2239 3405 4363
Size: 416 53 83 399 24 822 1166 958
Cuts at: 4363 442
Size: 1753
Fragments arranged by size:
1753 1166 958 822 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5674
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4334 1239
Size: 3095 2579
Bpu10I CC'TnA_GC
Cuts at: 2592 2592
Size: 5674
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3799 4664 4962 3799
Size: 865 298 4511
Fragments arranged by size:
4511 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4337 882
Size: 617 2838 2219
Fragments arranged by size:
2838 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3465 493
Size: 711 2261 2702
Fragments arranged by size:
2702 2261 711
BsaBI GATnn'nnATC
Cuts at: 2196 5668 2196
Size: 3472 2202
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2823 2944 3481 3863
Size: 53 83 186 936 1290 121 537 382
Cuts at: 3863 4498 275
Size: 635 1451
Fragments arranged by size:
1451 1290 936 635 537 382 186 121
83 53
BsaWI w'CCGG_w
Cuts at: 4107 4505 4779 4926 5337 4107
Size: 398 274 147 411 4444
Fragments arranged by size:
4444 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2900 2930 1178
Size: 30 165 30 1497 30 3922
Fragments arranged by size:
3922 1497 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2132 2135 2255 2276 2279 1186
Size: 946 3 120 21 3 4581
Fragments arranged by size:
4581 946 120 21 3 3
BseYI C'CCAG_C
Cuts at: 2043 2100 2180 2297 2722 2826 2831 4877 5373
Size: 57 80 117 425 104 5 2046 496
Cuts at: 5373 2043
Size: 2344
Fragments arranged by size:
2344 2046 496 425 117 104 80 57
5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2354 2363 2375 1163
Size: 573 618 9 12 4462
Fragments arranged by size:
4462 618 573 12 9
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2521 2737 3124 3885 4913 1540
Size: 275 186 520 216 387 761 1028 2301
Fragments arranged by size:
2301 1028 761 520 387 275 216 186
BsiHKAI G_wGCw'C
Cuts at: 2517 2618 2733 2820 3735 3820 4891 2517
Size: 101 115 87 915 85 1071 3300
Fragments arranged by size:
3300 1071 915 115 101 87 85
BsmI GAATG_Cn'
Cuts at: 3185 3278 3185
Size: 93 5581
BsmBI CGTCTCn'nnnn_
Cuts at: 2951 2951
Size: 5674
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2071 2113 2517 2618 2733 2820 2993
Size: 399 281 42 404 101 115 87 173
Cuts at: 2993 3735 3820 4891 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 404 399 281 173 115
101 87 85 42
BspEI T'CCGG_A
Cuts at: 5337 5337
Size: 5674
BspHI T'CATG_A
Cuts at: 3563 5293 3563
Size: 1730 3944
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2691 3029 844
Size: 1847 338 3489
Fragments arranged by size:
3489 1847 338
BsrBI CCG'CTC
Cuts at: 1394 1823 2125 2851 3561 1394
Size: 429 302 726 710 3507
Fragments arranged by size:
3507 726 710 429 302
BsrDI GCAATG_nn'
Cuts at: 65 1248 4169 4343 65
Size: 1183 2921 174 1396
Fragments arranged by size:
2921 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2351 2644 4318 4505 1700
Size: 110 541 293 1674 187 2869
Fragments arranged by size:
2869 1674 541 293 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5674
BssHII G'CGCG_C
Cuts at: 1925 2686 2740 1925
Size: 761 54 4859
Fragments arranged by size:
4859 761 54
BssSI C'ACGA_G
Cuts at: 3734 4746 3734
Size: 1012 4662
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2377 2482 794
Size: 1583 105 3986
Fragments arranged by size:
3986 1583 105
BstBI TT'CG_AA
Cuts at: 2586 2763 4478 2586
Size: 177 1715 3782
Fragments arranged by size:
3782 1715 177
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5674
BstYI r'GATC_y
Cuts at: 1972 3004 3371 3756 3773 5225 5311 5323 5669
Size: 1032 367 385 17 1452 86 12 346
Cuts at: 5669 1972
Size: 1977
Fragments arranged by size:
1977 1452 1032 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2316 2316
Size: 5674
BtgI C'CryG_G
Cuts at: 513 1065 2062 513
Size: 552 997 4125
Fragments arranged by size:
4125 997 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2481 2783 487
Size: 683 56 1255 302 3378
Fragments arranged by size:
3378 1255 683 302 56
BtsI GCAGTG_nn'
Cuts at: 3190 3984 4004 3190
Size: 794 20 4860
Fragments arranged by size:
4860 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5674
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5639
DraI TTT'AAA
Cuts at: 2978 2978
Size: 5674
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4681 809
Size: 507 3365 1802
Fragments arranged by size:
3365 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2059 2349 2518
Size: 54 1425 50 275 18 229 290 169
Cuts at: 2518 2770 3121 4010 5639 8
Size: 252 351 889 1629 43
Fragments arranged by size:
1629 1425 889 351 290 275 252 229
169 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2518 3121 1537
Size: 275 706 603 4090
Fragments arranged by size:
4090 706 603 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2253 2765 3604 1353
Size: 900 512 839 3423
Fragments arranged by size:
3423 900 839 512
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4261 4635 4781 387
Size: 1064 138 2672 374 146 1280
Fragments arranged by size:
2672 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2818 2818
Size: 5674
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2168 2558 3737 4334 5121 5389 1239
Size: 393 536 390 1179 597 787 268 1524
Fragments arranged by size:
1524 1179 787 597 536 393 390 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2486 2486
Size: 5674
EcoO109I rG'GnC_Cy
Cuts at: 2137 2749 2137
Size: 612 5062
EcoRI G'AATT_C
Cuts at: 2983 3361 2983
Size: 378 5296
FseI GG_CCGG'CC
Cuts at: 2355 2355
Size: 5674
FspI TGC'GCA
Cuts at: 4180 4180
Size: 5674
HaeII r_GCGC'y
Cuts at: 1678 2672 2696 2826 2838 4821 1678
Size: 994 24 130 12 1983 2531
Fragments arranged by size:
2531 1983 994 130 24 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2900 2932 4315 4347 4389
Size: 32 135 32 1328 32 1383 32 42
Cuts at: 4389 4421 1373
Size: 32 2626
Fragments arranged by size:
2626 1383 1328 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3018 3264 669
Size: 988 281 1080 246 3079
Fragments arranged by size:
3079 1080 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5674
HpaI GTT'AAC
Cuts at: 3264 3264
Size: 5674
KasI G'GCGC_C
Cuts at: 2822 2822
Size: 5674
KpnI G_GTAC'C
Cuts at: 2683 2999 2683
Size: 316 5358
MfeI C'AATT_G
Cuts at: 3273 3273
Size: 5674
MluI A'CGCG_T
Cuts at: 2394 5550 2394
Size: 3156 2518
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2379 4788 4972 1305
Size: 1074 2409 184 2007
Fragments arranged by size:
2409 2007 1074 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2061 10
Size: 54 1425 343 229 3623
Fragments arranged by size:
3623 1425 343 229 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2408 3632 3991 4150 518
Size: 648 1242 1224 359 159 2042
Fragments arranged by size:
2042 1242 1224 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2377 2440 2941 3090 3767 4915 5160 1606
Size: 771 63 501 149 677 1148 245 2120
Fragments arranged by size:
2120 1148 771 677 501 245 149 63
NaeI GCC'GGC
Cuts at: 2353 2646 2353
Size: 293 5381
NarI GG'CG_CC
Cuts at: 2823 2823
Size: 5674
NcoI C'CATG_G
Cuts at: 513 1065 2062 513
Size: 552 997 4125
Fragments arranged by size:
4125 997 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5674
NgoMIV G'CCGG_C
Cuts at: 2351 2644 2351
Size: 293 5381
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5674
NotI GC'GGCC_GC
Cuts at: 3121 3121
Size: 5674
NspI r_CATG'y
Cuts at: 1132 1229 3032 4577 1132
Size: 97 1803 1545 2229
Fragments arranged by size:
2229 1803 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2425 1348
Size: 1077 4597
PciI A'CATG_T
Cuts at: 4573 4573
Size: 5674
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2050 2662 1108
Size: 942 612 4120
Fragments arranged by size:
4120 942 612
PmeI GTTT'AAAC
Cuts at: 2978 2978
Size: 5674
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3743 3775 5256 5288 3743
Size: 32 1481 32 4129
Fragments arranged by size:
4129 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5674
PsiI TTA'TAA
Cuts at: 3244 3244
Size: 5674
PstI C_TGCA'G
Cuts at: 830 2160 2487 3026 830
Size: 1330 327 539 3478
Fragments arranged by size:
3478 1330 539 327
PvuI CG_AT'CG
Cuts at: 2001 2737 2001
Size: 736 4938
PvuII CAG'CTG
Cuts at: 2377 2440 2377
Size: 63 5611
SacI G_AGCT'C
Cuts at: 2820 2820
Size: 5674
SalI G'TCGA_C
Cuts at: 1936 3016 1936
Size: 1080 4594
SbfI CC_TGCA'GG
Cuts at: 2487 3026 2487
Size: 539 5135
ScaI AGT'ACT
Cuts at: 3922 5634 3922
Size: 1712 3962
SfcI C'TryA_G
Cuts at: 826 1045 2156 2483 3022 4157 4838 5029 826
Size: 219 1111 327 539 1135 681 191 1471
Fragments arranged by size:
1471 1135 1111 681 539 327 219 191
SfoI GGC'GCC
Cuts at: 2824 2824
Size: 5674
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5674
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5674
SmaI CCC'GGG
Cuts at: 1872 3001 1872
Size: 1129 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2882 3778 4679 4941 820
Size: 197 926 939 896 901 262 1553
Fragments arranged by size:
1553 939 926 901 896 262 197
SnaBI TAC'GTA
Cuts at: 493 3465 493
Size: 2972 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5674
SphI G_CATG'C
Cuts at: 1132 3032 1132
Size: 1900 3774
SspI AAT'ATT
Cuts at: 5 52 3598 5
Size: 47 3546 2081
Fragments arranged by size:
3546 2081 47
StuI AGG'CCT
Cuts at: 1517 2367 2903 1517
Size: 850 536 4288
Fragments arranged by size:
4288 850 536
StyI C'CwwG_G
Cuts at: 513 1065 2062 2167 3113 513
Size: 552 997 105 946 3074
Fragments arranged by size:
3074 997 946 552 105
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3701 3718 3871 4056 4501 325
Size: 1255 2121 17 153 185 445 1498
Fragments arranged by size:
2121 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3920 5632 96
Size: 274 80 33 51 1443 1943 1712 138
Fragments arranged by size:
1943 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5634 5666 5634
Size: 32 5642
Tth111I GACn'n_nGTC
Cuts at: 1316 2324 1316
Size: 1008 4666
XbaI T'CTAG_A
Cuts at: 3010 3010
Size: 5674
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2870 2870
Size: 5674
XhoI C'TCGA_G
Cuts at: 1943 2882 1943
Size: 939 4735
XmaI C'CCGG_G
Cuts at: 1870 2999 1870
Size: 1129 4545
XmnI GAAnn'nnTTC
Cuts at: 3803 3803
Size: 5674
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3482 276
Size: 53 83 186 936 1948 2468
Fragments arranged by size:
2468 1948 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AscI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PstI
PvuI PvuII SacI SalI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI BaeI BbvCI BfrBI BplI
BsiWI BstXI BstZ17I DraIII EcoRV FalI FspAI NruI
NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI
RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI