Restriction map for
pFN21AB3427
(Circular) (Six-base) MAPSORT of: pFN21AB3427.seq Check: 4036 from: 1 to: 5980
pFN21AB3427 5980 bp
With 182 enzymes: SgfI *
February 27, 2009 14:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3790 278
Size: 53 83 186 936 2254 2468
Fragments arranged by size:
2468 2254 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2458 3323 1937
Size: 521 865 4594
Fragments arranged by size:
4594 865 521
Acc65I G'GTAC_C
Cuts at: 2603 3301 2603
Size: 698 5282
AclI AA'CG_TT
Cuts at: 1563 2251 2515 2911 4107 4480 1563
Size: 688 264 396 1196 373 3063
Fragments arranged by size:
3063 1196 688 396 373 264
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3102 4043 5427 5695 1632
Size: 1470 941 1384 268 1917
Fragments arranged by size:
1917 1470 1384 941 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5783
AflIII A'CryG_T
Cuts at: 1203 4879 5856 1203
Size: 3676 977 1327
Fragments arranged by size:
3676 1327 977
AgeI A'CCGG_T
Cuts at: 4811 4811
Size: 5980
AhdI GACnn_n'nnGTC
Cuts at: 4709 4709
Size: 5980
AleI CACnn'nnGTG
Cuts at: 1166 2328 1166
Size: 1162 4818
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5980
AlwNI CAG_nnn'CTG
Cuts at: 2972 3723 5295 2972
Size: 751 1572 3657
Fragments arranged by size:
3657 1572 751
ApaLI G'TGCA_C
Cuts at: 2279 2789 4037 5193 2279
Size: 510 1248 1156 3066
Fragments arranged by size:
3066 1248 1156 510
ApoI r'AATT_y
Cuts at: 1493 2108 3289 3515 3667 5776 1493
Size: 615 1181 226 152 2109 1697
Fragments arranged by size:
2109 1697 1181 615 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4534 160
Size: 872 3502 1606
Fragments arranged by size:
3502 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5980
AvaI C'yCGr_G
Cuts at: 1870 1943 3305 1870
Size: 73 1362 4545
Fragments arranged by size:
4545 1362 73
BamHI G'GATC_C
Cuts at: 3310 3677 3310
Size: 367 5613
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2603 3301 4756 618
Size: 325 217 627 816 698 1455 1842
Fragments arranged by size:
1842 1455 816 698 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3299 1391
Size: 1908 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5009
BbvCI CC'TCA_GC
Cuts at: 2238 2238
Size: 5980
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4171 4205 1903
Size: 34 2234 34 3678
Fragments arranged by size:
3678 2234 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3234 3872 5082 3234
Size: 638 1210 4132
Fragments arranged by size:
4132 1210 638
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5980
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 4591 136
Size: 107 122 71 631 605 337 2582 1525
Fragments arranged by size:
2582 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 5975 5975
Size: 5980
BlpI GC'TnA_GC
Cuts at: 3397 3397
Size: 5980
Bme1580I G_kGCm'C
Cuts at: 1790 2283 2793 4041 5197 1790
Size: 493 510 1248 1156 2573
Fragments arranged by size:
2573 1248 1156 510 493
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5980
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3711 4669 442
Size: 416 53 83 399 24 2294 958 1753
Fragments arranged by size:
2294 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2775 1056
Size: 1719 4261
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2034 2231 2537 3201 4640 1239
Size: 795 197 306 664 1439 2579
Fragments arranged by size:
2579 1439 795 664 306 197
Bpu10I CC'TnA_GC
Cuts at: 2238 2442 2238
Size: 204 5776
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2813 2854 4105 4970 5268 2813
Size: 41 1251 865 298 3525
Fragments arranged by size:
3525 1251 865 298 41
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4643 882
Size: 617 3144 2219
Fragments arranged by size:
3144 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2278 3771 493
Size: 711 1074 1493 2702
Fragments arranged by size:
2702 1493 1074 711
BsaBI GATnn'nnATC
Cuts at: 5974 5974
Size: 5980
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3787 4169 4804 275
Size: 53 83 186 936 2254 382 635 1451
Fragments arranged by size:
2254 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2112 4413 4811 5085 5232 5643 2112
Size: 2301 398 274 147 411 2449
Fragments arranged by size:
2449 2301 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5755
Fragments arranged by size:
5755 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3230 1186
Size: 2044 3936
BseYI C'CCAG_C
Cuts at: 5183 5679 5183
Size: 496 5484
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2475 3218 1163
Size: 573 739 743 3925
Fragments arranged by size:
3925 743 739 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3430 4191 5219 1540
Size: 275 186 1429 761 1028 2301
Fragments arranged by size:
2301 1429 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2283 2793 4041 4126 5197 2283
Size: 510 1248 85 1071 3066
Fragments arranged by size:
3066 1248 1071 510 85
BsmI GAATG_Cn'
Cuts at: 3120 3491 3584 3120
Size: 371 93 5516
Fragments arranged by size:
5516 371 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2283 2793 3299 4041 4126 5197 1391
Size: 399 493 510 506 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 510 506 493 399 85
BspEI T'CCGG_A
Cuts at: 2112 5643 2112
Size: 3531 2449
BspHI T'CATG_A
Cuts at: 3869 5599 3869
Size: 1730 4250
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2400 2589 3335 844
Size: 1556 189 746 3489
Fragments arranged by size:
3489 1556 746 189
BsrBI CCG'CTC
Cuts at: 1394 1823 2046 3867 1394
Size: 429 223 1821 3507
Fragments arranged by size:
3507 1821 429 223
BsrDI GCAATG_nn'
Cuts at: 65 1248 4475 4649 65
Size: 1183 3227 174 1396
Fragments arranged by size:
3227 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4624 4811 1700
Size: 110 2814 187 2869
Fragments arranged by size:
2869 2814 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5980
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5980
BssSI C'ACGA_G
Cuts at: 4040 5052 4040
Size: 1012 4968
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3133 794
Size: 2339 3641
BstBI TT'CG_AA
Cuts at: 4784 4784
Size: 5980
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5980
BstYI r'GATC_y
Cuts at: 1972 2536 2548 3018 3310 3677 4062 4079 5531
Size: 564 12 470 292 367 385 17 1452
Cuts at: 5531 5617 5629 5975 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 564 470 385 367 346 292
86 17 12 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3168 513
Size: 552 939 1164 3325
Fragments arranged by size:
3325 1164 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2891 487
Size: 683 56 1665 3576
Fragments arranged by size:
3576 1665 683 56
BtsI GCAGTG_nn'
Cuts at: 2293 3496 4290 4310 2293
Size: 1203 794 20 3963
Fragments arranged by size:
3963 1203 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5980
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5945
DraI TTT'AAA
Cuts at: 3284 3284
Size: 5980
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4987 809
Size: 507 3671 1802
Fragments arranged by size:
3671 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2290 3027 3144
Size: 54 1425 50 275 18 460 737 117
Cuts at: 3144 3165 3427 4316 5945 8
Size: 21 262 889 1629 43
Fragments arranged by size:
1629 1425 889 737 460 275 262 117
54 50 43 21 18
EagI C'GGCC_G
Cuts at: 1537 1812 3427 1537
Size: 275 1615 4090
Fragments arranged by size:
4090 1615 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2238 3207 3910 1353
Size: 885 969 703 3423
Fragments arranged by size:
3423 969 885 703
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2159 4567 4941 5087 387
Size: 1064 138 570 2408 374 146 1280
Fragments arranged by size:
2408 1280 1064 570 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2034 2231 2537 3102 3201 4043 4640
Size: 393 402 197 306 565 99 842 597
Cuts at: 4640 5427 5695 1239
Size: 787 268 1524
Fragments arranged by size:
1524 842 787 597 565 402 393 306
268 197 99
EcoRI G'AATT_C
Cuts at: 3289 3667 3289
Size: 378 5602
FspI TGC'GCA
Cuts at: 4486 4486
Size: 5980
HaeII r_GCGC'y
Cuts at: 1678 5127 1678
Size: 3449 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2186 2218 2521 2553 2697
Size: 32 135 32 614 32 303 32 144
Cuts at: 2697 2729 4621 4653 4695 4727 1373
Size: 32 1892 32 42 32 2626
Fragments arranged by size:
2626 1892 614 303 144 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3324 3570 669
Size: 988 281 1386 246 3079
Fragments arranged by size:
3079 1386 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2405 748
Size: 1657 4323
HpaI GTT'AAC
Cuts at: 3570 3570
Size: 5980
KpnI G_GTAC'C
Cuts at: 2607 3305 2607
Size: 698 5282
MfeI C'AATT_G
Cuts at: 3579 3579
Size: 5980
MluI A'CGCG_T
Cuts at: 5856 5856
Size: 5980
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2329 5094 5278 1305
Size: 1024 2765 184 2007
Fragments arranged by size:
2765 2007 1024 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2292 3146 3167 10
Size: 54 1425 343 460 854 21 2823
Fragments arranged by size:
2823 1425 854 460 343 54 21
MslI CAynn'nnrTG
Cuts at: 518 1166 2328 3161 3167 3938 4297 4456 518
Size: 648 1162 833 6 771 359 159 2042
Fragments arranged by size:
2042 1162 833 771 648 359 159 6
MspA1I CmG'CkG
Cuts at: 1606 2128 2237 2497 3396 4073 5221 5466 1606
Size: 522 109 260 899 677 1148 245 2120
Fragments arranged by size:
2120 1148 899 677 522 260 245 109
NcoI C'CATG_G
Cuts at: 513 1065 2004 3168 513
Size: 552 939 1164 3325
Fragments arranged by size:
3325 1164 939 552
NdeI CA'TA_TG
Cuts at: 387 3241 387
Size: 2854 3126
NheI G'CTAG_C
Cuts at: 1052 2771 1052
Size: 1719 4261
NotI GC'GGCC_GC
Cuts at: 3427 3427
Size: 5980
NspI r_CATG'y
Cuts at: 1132 1229 3338 4883 1132
Size: 97 2109 1545 2229
Fragments arranged by size:
2229 2109 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5980
PciI A'CATG_T
Cuts at: 4879 4879
Size: 5980
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5980
PfoI T'CCnGG_A
Cuts at: 2190 2190
Size: 5980
PmeI GTTT'AAAC
Cuts at: 3284 3284
Size: 5980
PmlI CAC'GTG
Cuts at: 2278 2278
Size: 5980
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4049 4081 5562 5594 4049
Size: 32 1481 32 4435
Fragments arranged by size:
4435 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2945 1613
Size: 1332 4648
PsiI TTA'TAA
Cuts at: 3550 3550
Size: 5980
PstI C_TGCA'G
Cuts at: 830 2314 2634 2921 3072 3332 830
Size: 1484 320 287 151 260 3478
Fragments arranged by size:
3478 1484 320 287 260 151
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5980
PvuII CAG'CTG
Cuts at: 2128 2237 2497 2128
Size: 109 260 5611
Fragments arranged by size:
5611 260 109
SalI G'TCGA_C
Cuts at: 1936 3322 1936
Size: 1386 4594
SbfI CC_TGCA'GG
Cuts at: 3332 3332
Size: 5980
ScaI AGT'ACT
Cuts at: 2757 4228 5940 2757
Size: 1471 1712 2797
Fragments arranged by size:
2797 1712 1471
SfcI C'TryA_G
Cuts at: 826 1045 2310 2630 2917 2960 3068 3328 4463
Size: 219 1265 320 287 43 108 260 1135
Cuts at: 4463 5144 5335 826
Size: 681 191 1471
Fragments arranged by size:
1471 1265 1135 681 320 287 260 219
191 108 43
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5980
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5980
SmaI CCC'GGG
Cuts at: 1872 3307 1872
Size: 1435 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2828 2869 4084 4985 5247 820
Size: 197 926 885 41 1215 901 262 1553
Fragments arranged by size:
1553 1215 926 901 885 262 197 41
SnaBI TAC'GTA
Cuts at: 493 3771 493
Size: 3278 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5980
SphI G_CATG'C
Cuts at: 1132 3338 1132
Size: 2206 3774
SspI AAT'ATT
Cuts at: 5 52 3904 5
Size: 47 3852 2081
Fragments arranged by size:
3852 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5980
StyI C'CwwG_G
Cuts at: 513 1065 2004 2326 3168 3419 513
Size: 552 939 322 842 251 3074
Fragments arranged by size:
3074 939 842 552 322 251
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4007 4024 4177 4362 4807 325
Size: 1255 2427 17 153 185 445 1498
Fragments arranged by size:
2427 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2755 4226 5938
Size: 274 80 33 51 1443 778 1471 1712
Cuts at: 5938 96
Size: 138
Fragments arranged by size:
1712 1471 1443 778 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3078 3110 5940 5972 3078
Size: 32 2830 32 3086
Fragments arranged by size:
3086 2830 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5980
XbaI T'CTAG_A
Cuts at: 3316 3316
Size: 5980
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5980
XmaI C'CCGG_G
Cuts at: 1870 3305 1870
Size: 1435 4545
XmnI GAAnn'nnTTC
Cuts at: 4109 4109
Size: 5980
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3788 276
Size: 53 83 186 936 2254 2468
Fragments arranged by size:
2468 2254 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PpuMI PspOMI
PspXI PsrI RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI XcmI