Restriction map for
pFN21AB3424
(Circular) (Six-base) MAPSORT of: pFN21AB3424.seq Check: 3543 from: 1 to: 6025
pFN21AB3424 6025 bp
With 182 enzymes: SgfI *
October 20, 2009 10:34 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3835 278
Size: 53 83 186 936 2299 2468
Fragments arranged by size:
2468 2299 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2375 2421 3368 1937
Size: 438 46 947 4594
Fragments arranged by size:
4594 947 438 46
Acc65I G'GTAC_C
Cuts at: 3346 3346
Size: 6025
AclI AA'CG_TT
Cuts at: 1563 4152 4525 1563
Size: 2589 373 3063
Fragments arranged by size:
3063 2589 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2213 2847 4088 5472 5740 1632
Size: 581 634 1241 1384 268 1917
Fragments arranged by size:
1917 1384 1241 634 581 268
AflII C'TTAA_G
Cuts at: 820 1017 3223 820
Size: 197 2206 3622
Fragments arranged by size:
3622 2206 197
AflIII A'CryG_T
Cuts at: 1203 4924 5901 1203
Size: 3721 977 1327
Fragments arranged by size:
3721 1327 977
AgeI A'CCGG_T
Cuts at: 4856 4856
Size: 6025
AhdI GACnn_n'nnGTC
Cuts at: 4754 4754
Size: 6025
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6025
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6025
AlwNI CAG_nnn'CTG
Cuts at: 2138 3209 3768 5340 2138
Size: 1071 559 1572 2823
Fragments arranged by size:
2823 1572 1071 559
ApaI G_GGCC'C
Cuts at: 2796 2796
Size: 6025
ApaLI G'TGCA_C
Cuts at: 4082 5238 4082
Size: 1156 4869
ApoI r'AATT_y
Cuts at: 1493 2234 2583 2916 3334 3560 3712 5821 1493
Size: 741 349 333 418 226 152 2109 1697
Fragments arranged by size:
2109 1697 741 418 349 333 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4579 160
Size: 872 3547 1606
Fragments arranged by size:
3547 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6025
AvaI C'yCGr_G
Cuts at: 1870 1943 3350 1870
Size: 73 1407 4545
Fragments arranged by size:
4545 1407 73
AvrII C'CTAG_G
Cuts at: 2782 2782
Size: 6025
BamHI G'GATC_C
Cuts at: 2506 3016 3355 3722 2506
Size: 510 339 367 4809
Fragments arranged by size:
4809 510 367 339
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3302 3346 4801 618
Size: 325 217 627 1515 44 1455 1842
Fragments arranged by size:
1842 1515 1455 627 325 217 44
BanII G_rGCy'C
Cuts at: 1391 2390 2796 3344 1391
Size: 999 406 548 4072
Fragments arranged by size:
4072 999 548 406
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3261 928
Size: 971 1362 3692
Fragments arranged by size:
3692 1362 971
BbvCI CC'TCA_GC
Cuts at: 2734 2734
Size: 6025
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4216 4250 1903
Size: 34 2279 34 3678
Fragments arranged by size:
3678 2279 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3917 5127 3917
Size: 1210 4815
BclI T'GATC_A
Cuts at: 1548 3153 1548
Size: 1605 4420
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4636 136
Size: 107 122 71 631 605 2964 1525
Fragments arranged by size:
2964 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6020 6020
Size: 6025
BlpI GC'TnA_GC
Cuts at: 3442 3442
Size: 6025
Bme1580I G_kGCm'C
Cuts at: 1790 2796 4086 5242 1790
Size: 1006 1290 1156 2573
Fragments arranged by size:
2573 1290 1156 1006
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6025
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3756 4714 442
Size: 416 53 83 399 24 2339 958 1753
Fragments arranged by size:
2339 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6025
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2058 4685 1239
Size: 819 2627 2579
Fragments arranged by size:
2627 2579 819
Bpu10I CC'TnA_GC
Cuts at: 2734 2734
Size: 6025
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2767 4150 5015 5313 2767
Size: 1383 865 298 3479
Fragments arranged by size:
3479 1383 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2543 4688 882
Size: 617 1044 2145 2219
Fragments arranged by size:
2219 2145 1044 617
BsaAI yAC'GTr
Cuts at: 493 1204 3816 493
Size: 711 2612 2702
Fragments arranged by size:
2702 2612 711
BsaBI GATnn'nnATC
Cuts at: 2358 6019 2358
Size: 3661 2364
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2868 3832 4214 4849
Size: 53 83 186 936 1335 964 382 635
Cuts at: 4849 275
Size: 1451
Fragments arranged by size:
1451 1335 964 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4458 4856 5130 5277 5688 4458
Size: 398 274 147 411 4795
Fragments arranged by size:
4795 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5800
Fragments arranged by size:
5800 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2297 1186
Size: 1111 4914
BseYI C'CCAG_C
Cuts at: 5228 5724 5228
Size: 496 5529
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5452
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3475 4236 5264 1540
Size: 275 186 1474 761 1028 2301
Fragments arranged by size:
2301 1474 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4086 4171 5242 4086
Size: 85 1071 4869
Fragments arranged by size:
4869 1071 85
BsiWI C'GTAC_G
Cuts at: 2803 2803
Size: 6025
BsmI GAATG_Cn'
Cuts at: 2406 3536 3629 2406
Size: 1130 93 4802
Fragments arranged by size:
4802 1130 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2390 2796 3344 4086 4171 5242 1391
Size: 399 600 406 548 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 600 548 406 399 85
BspEI T'CCGG_A
Cuts at: 5688 5688
Size: 6025
BspHI T'CATG_A
Cuts at: 3914 5644 3914
Size: 1730 4295
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3380 844
Size: 2536 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3912 1394
Size: 429 2089 3507
Fragments arranged by size:
3507 2089 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4520 4694 65
Size: 1183 3272 174 1396
Fragments arranged by size:
3272 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2178 4669 4856 1700
Size: 110 368 2491 187 2869
Fragments arranged by size:
2869 2491 368 187 110
BsrGI T'GTAC_A
Cuts at: 96 3200 96
Size: 3104 2921
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6025
BssSI C'ACGA_G
Cuts at: 4085 5097 4085
Size: 1012 5013
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6025
BstBI TT'CG_AA
Cuts at: 4829 4829
Size: 6025
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6025
BstXI CCAn_nnnn'nTGG
Cuts at: 2954 2954
Size: 6025
BstYI r'GATC_y
Cuts at: 1972 2428 2506 3016 3355 3722 4107 4124 5576
Size: 456 78 510 339 367 385 17 1452
Cuts at: 5576 5662 5674 6020 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 510 456 385 367 346 339
86 78 17 12
Bsu36I CC'TnA_GG
Cuts at: 2661 3309 2661
Size: 648 5377
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4534
Fragments arranged by size:
4534 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5286
Fragments arranged by size:
5286 683 56
BtsI GCAGTG_nn'
Cuts at: 3541 4335 4355 3541
Size: 794 20 5211
Fragments arranged by size:
5211 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6025
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2082 2117 2884 2919 529
Size: 35 1518 35 767 35 3635
Fragments arranged by size:
3635 1518 767 35 35 35
DraI TTT'AAA
Cuts at: 3329 3329
Size: 6025
DraIII CAC_nnn'GTG
Cuts at: 2175 2470 2175
Size: 295 5730
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2323 5032 809
Size: 507 1007 2709 1802
Fragments arranged by size:
2709 1802 1007 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2991 3472 4361
Size: 54 1425 50 275 18 1161 481 889
Cuts at: 4361 5990 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1161 889 481 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3472 1537
Size: 275 1660 4090
Fragments arranged by size:
4090 1660 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2337 2634 3955 1353
Size: 984 297 1321 3423
Fragments arranged by size:
3423 1321 984 297
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4612 4986 5132 387
Size: 1064 138 3023 374 146 1280
Fragments arranged by size:
3023 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2058 2213 2847 4088 4685 5472 5740
Size: 393 426 155 634 1241 597 787 268
Cuts at: 5740 1239
Size: 1524
Fragments arranged by size:
1524 1241 787 634 597 426 393 268
155
EcoNI CCTnn'n_nnAGG
Cuts at: 2691 2691
Size: 6025
EcoO109I rG'GnC_Cy
Cuts at: 2316 2539 2316
Size: 223 5802
EcoRI G'AATT_C
Cuts at: 2234 3334 3712 2234
Size: 1100 378 4547
Fragments arranged by size:
4547 1100 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2234 3112 2234
Size: 878 5147
FspI TGC'GCA
Cuts at: 4531 4531
Size: 6025
HaeII r_GCGC'y
Cuts at: 1678 5172 1678
Size: 3494 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2011 2043 2276 2308 3158
Size: 32 135 32 439 32 233 32 850
Cuts at: 3158 3190 4666 4698 4740 4772 1373
Size: 32 1476 32 42 32 2626
Fragments arranged by size:
2626 1476 850 439 233 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3369 3615 669
Size: 988 281 1431 246 3079
Fragments arranged by size:
3079 1431 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6025
HpaI GTT'AAC
Cuts at: 3615 3615
Size: 6025
KpnI G_GTAC'C
Cuts at: 3350 3350
Size: 6025
MfeI C'AATT_G
Cuts at: 3624 3624
Size: 6025
MluI A'CGCG_T
Cuts at: 5901 5901
Size: 6025
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2553 2913 2931 5139 5323 1305
Size: 1248 360 18 2208 184 2007
Fragments arranged by size:
2208 2007 1248 360 184 18
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2993 10
Size: 54 1425 343 1161 3042
Fragments arranged by size:
3042 1425 1161 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2522 3983 4342 4501 518
Size: 648 1356 1461 359 159 2042
Fragments arranged by size:
2042 1461 1356 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3441 4118 5266 5511 1606
Size: 1835 677 1148 245 2120
Fragments arranged by size:
2120 1835 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4534
Fragments arranged by size:
4534 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6025
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6025
NotI GC'GGCC_GC
Cuts at: 3472 3472
Size: 6025
NspI r_CATG'y
Cuts at: 1132 1229 2477 2837 3383 4928 1132
Size: 97 1248 360 546 1545 2229
Fragments arranged by size:
2229 1545 1248 546 360 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6025
PciI A'CATG_T
Cuts at: 4924 4924
Size: 6025
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6025
PfoI T'CCnGG_A
Cuts at: 2035 2035
Size: 6025
PmeI GTTT'AAAC
Cuts at: 3329 3329
Size: 6025
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4094 4126 5607 5639 4094
Size: 32 1481 32 4480
Fragments arranged by size:
4480 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2316 2316
Size: 6025
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6025
PsiI TTA'TAA
Cuts at: 3288 3595 3288
Size: 307 5718
PspOMI G'GGCC_C
Cuts at: 2792 2792
Size: 6025
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2073 2105 2073
Size: 32 5993
PstI C_TGCA'G
Cuts at: 830 3377 830
Size: 2547 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6025
SalI G'TCGA_C
Cuts at: 1936 3367 1936
Size: 1431 4594
SanDI GG'GwC_CC
Cuts at: 2316 2316
Size: 6025
SbfI CC_TGCA'GG
Cuts at: 3377 3377
Size: 6025
ScaI AGT'ACT
Cuts at: 4273 5985 4273
Size: 1712 4313
SfcI C'TryA_G
Cuts at: 826 1045 3373 4508 5189 5380 826
Size: 219 2328 1135 681 191 1471
Fragments arranged by size:
2328 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6025
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6025
SmaI CCC'GGG
Cuts at: 1872 3352 1872
Size: 1480 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2746 3223 4129 5030 5292 820
Size: 197 926 803 477 906 901 262 1553
Fragments arranged by size:
1553 926 906 901 803 477 262 197
SnaBI TAC'GTA
Cuts at: 493 3816 493
Size: 3323 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6025
SphI G_CATG'C
Cuts at: 1132 2477 3383 1132
Size: 1345 906 3774
Fragments arranged by size:
3774 1345 906
SspI AAT'ATT
Cuts at: 5 52 2633 3949 5
Size: 47 2581 1316 2081
Fragments arranged by size:
2581 2081 1316 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6025
StyI C'CwwG_G
Cuts at: 513 1065 2004 2337 2782 3464 513
Size: 552 939 333 445 682 3074
Fragments arranged by size:
3074 939 682 552 445 333
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2440 2636 4052 4069 4222 4407 4852
Size: 1255 860 196 1416 17 153 185 445
Cuts at: 4852 325
Size: 1498
Fragments arranged by size:
1498 1416 1255 860 445 196 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3200 4271 5983
Size: 274 80 33 51 1443 1223 1071 1712
Cuts at: 5983 96
Size: 138
Fragments arranged by size:
1712 1443 1223 1071 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5985 6017 5985
Size: 32 5993
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6025
XbaI T'CTAG_A
Cuts at: 3361 3361
Size: 6025
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6025
XmaI C'CCGG_G
Cuts at: 1870 3350 1870
Size: 1480 4545
XmnI GAAnn'nnTTC
Cuts at: 2769 3279 4154 2769
Size: 510 875 4640
Fragments arranged by size:
4640 875 510
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3833 276
Size: 53 83 186 936 2299 2468
Fragments arranged by size:
2468 2299 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI
PstI PvuI SalI SanDI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI BaeI BbeI BfrBI BplI
BsmBI BstZ17I EcoICRI EcoRV FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PspXI PvuII
RsrII SacI SacII SapI SexAI SfiI SfoI SrfI
SwaI XcmI