Restriction map for
pFN21AB3414
(Circular) (Six-base) MAPSORT of: pFN21AB3414.seq Check: 6872 from: 1 to: 7333
pFN21AB3414 7333 bp
With 182 enzymes: SgfI *
February 27, 2009 14:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2653 3083 2653
Size: 430 6903
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5143 278
Size: 53 83 186 936 3607 2468
Fragments arranged by size:
3607 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2179 2450 3345 4676 1937
Size: 242 271 895 1331 4594
Fragments arranged by size:
4594 1331 895 271 242
Acc65I G'GTAC_C
Cuts at: 3147 4654 3147
Size: 1507 5826
AclI AA'CG_TT
Cuts at: 1563 5460 5833 1563
Size: 3897 373 3063
Fragments arranged by size:
3897 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2880 3345 3459 3674 3728 3752 4205 4569
Size: 1248 465 114 215 54 24 453 364
Cuts at: 4569 4640 5396 6780 7048 1632
Size: 71 756 1384 268 1917
Fragments arranged by size:
1917 1384 1248 756 465 453 364 268
215 114 71 54 24
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7136
AflIII A'CryG_T
Cuts at: 1203 3100 6232 7209 1203
Size: 1897 3132 977 1327
Fragments arranged by size:
3132 1897 1327 977
AgeI A'CCGG_T
Cuts at: 6164 6164
Size: 7333
AhdI GACnn_n'nnGTC
Cuts at: 2273 3745 6062 2273
Size: 1472 2317 3544
Fragments arranged by size:
3544 2317 1472
AleI CACnn'nnGTG
Cuts at: 1166 3908 1166
Size: 2742 4591
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3070 1184
Size: 1886 5447
AlwNI CAG_nnn'CTG
Cuts at: 2725 2968 3370 4144 5076 6648 2725
Size: 243 402 774 932 1572 3410
Fragments arranged by size:
3410 1572 932 774 402 243
ApaLI G'TGCA_C
Cuts at: 5390 6546 5390
Size: 1156 6177
ApoI r'AATT_y
Cuts at: 1493 2846 3306 3929 4099 4388 4642 4868 5020
Size: 1353 460 623 170 289 254 226 152
Cuts at: 5020 7129 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1353 623 460 289 254 226
170 152
AseI AT'TA_AT
Cuts at: 160 1032 3251 5887 160
Size: 872 2219 2636 1606
Fragments arranged by size:
2636 2219 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7333
AvaI C'yCGr_G
Cuts at: 1870 1943 2024 4658 1870
Size: 73 81 2634 4545
Fragments arranged by size:
4545 2634 81 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2685 2718 2948 2981 2685
Size: 33 230 33 7037
Fragments arranged by size:
7037 230 33 33
BamHI G'GATC_C
Cuts at: 4663 5030 4663
Size: 367 6966
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3147 4654 6109 618
Size: 325 217 627 1360 1507 1455 1842
Fragments arranged by size:
1842 1507 1455 1360 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3201 4652 1391
Size: 1810 1451 4072
Fragments arranged by size:
4072 1810 1451
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2036 4571 928
Size: 971 137 2535 3690
Fragments arranged by size:
3690 2535 971 137
BbvCI CC'TCA_GC
Cuts at: 2397 2397
Size: 7333
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5524 5558 1903
Size: 34 3587 34 3678
Fragments arranged by size:
3678 3587 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5225 6435 5225
Size: 1210 6123
BclI T'GATC_A
Cuts at: 1548 2013 3472 1548
Size: 465 1459 5409
Fragments arranged by size:
5409 1459 465
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3893 5944 136
Size: 107 122 71 631 605 2221 2051 1525
Fragments arranged by size:
2221 2051 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3810 7328 3810
Size: 3518 3815
BlpI GC'TnA_GC
Cuts at: 4750 4750
Size: 7333
Bme1580I G_kGCm'C
Cuts at: 1790 4045 5394 6550 1790
Size: 2255 1349 1156 2573
Fragments arranged by size:
2573 2255 1349 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7333
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2363 3195 3258
Size: 416 53 83 399 24 946 832 63
Cuts at: 3258 5064 6022 442
Size: 1806 958 1753
Fragments arranged by size:
1806 1753 958 946 832 416 399 83
63 53 24
BmtI G_CTAG'C
Cuts at: 1056 2582 1056
Size: 1526 5807
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2072 2099 3412 4440 5993 1239
Size: 833 27 1313 1028 1553 2579
Fragments arranged by size:
2579 1553 1313 1028 833 27
Bpu10I CC'TnA_GC
Cuts at: 2397 2925 4286 2397
Size: 528 1361 5444
Fragments arranged by size:
5444 1361 528
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3920 5458 6323 6621 3920
Size: 1538 865 298 4632
Fragments arranged by size:
4632 1538 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3386 5996 882
Size: 617 1887 2610 2219
Fragments arranged by size:
2610 2219 1887 617
BsaAI yAC'GTr
Cuts at: 493 1204 5124 493
Size: 711 3920 2702
Fragments arranged by size:
3920 2702 711
BsaBI GATnn'nnATC
Cuts at: 3633 4428 7327 3633
Size: 795 2899 3639
Fragments arranged by size:
3639 2899 795
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5140 5522 6157 275
Size: 53 83 186 936 3607 382 635 1451
Fragments arranged by size:
3607 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5766 6164 6438 6585 6996 5766
Size: 398 274 147 411 6103
Fragments arranged by size:
6103 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2023 2053 3090 3120 1178
Size: 30 165 30 620 30 1037 30 5391
Fragments arranged by size:
5391 1037 620 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3592 4194 1186
Size: 2406 602 4325
Fragments arranged by size:
4325 2406 602
BseYI C'CCAG_C
Cuts at: 3417 3860 6536 7032 3417
Size: 443 2676 496 3718
Fragments arranged by size:
3718 2676 496 443
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2379 1163
Size: 573 643 6117
Fragments arranged by size:
6117 643 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2047 2091 4783 5544 6572 1540
Size: 275 186 46 44 2692 761 1028 2301
Fragments arranged by size:
2692 2301 1028 761 275 186 46 44
BsiHKAI G_wGCw'C
Cuts at: 2439 2931 2959 3716 5394 5479 6550 2439
Size: 492 28 757 1678 85 1071 3222
Fragments arranged by size:
3222 1678 1071 757 492 85 28
BsmI GAATG_Cn'
Cuts at: 2596 4844 4937 2596
Size: 2248 93 4992
Fragments arranged by size:
4992 2248 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2439 2931 2959 3201 3716 4045 4652
Size: 399 649 492 28 242 515 329 607
Cuts at: 4652 5394 5479 6550 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 649 607 515 492 399
329 242 85 28
BspEI T'CCGG_A
Cuts at: 6996 6996
Size: 7333
BspHI T'CATG_A
Cuts at: 5222 6952 5222
Size: 1730 5603
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2109 2653 3083 4688 844
Size: 1265 544 430 1605 3489
Fragments arranged by size:
3489 1605 1265 544 430
BsrBI CCG'CTC
Cuts at: 1394 1823 5220 1394
Size: 429 3397 3507
Fragments arranged by size:
3507 3397 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2811 4413 5828 6002 65
Size: 1183 1563 1602 1415 174 1396
Fragments arranged by size:
1602 1563 1415 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2316 5977 6164 1700
Size: 110 506 3661 187 2869
Fragments arranged by size:
3661 2869 506 187 110
BsrGI T'GTAC_A
Cuts at: 96 4481 96
Size: 4385 2948
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7333
BssSI C'ACGA_G
Cuts at: 5393 6405 5393
Size: 1012 6321
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3464 794
Size: 2670 4663
BstBI TT'CG_AA
Cuts at: 6137 6137
Size: 7333
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7333
BstXI CCAn_nnnn'nTGG
Cuts at: 2134 4418 2134
Size: 2284 5049
BstYI r'GATC_y
Cuts at: 1972 2209 2364 3810 4663 5030 5415 5432 6884
Size: 237 155 1446 853 367 385 17 1452
Cuts at: 6884 6970 6982 7328 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1446 853 385 367 346 237
155 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3575 3575
Size: 7333
BtgI C'CryG_G
Cuts at: 513 1065 2004 2091 513
Size: 552 939 87 5755
Fragments arranged by size:
5755 939 552 87
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6594
Fragments arranged by size:
6594 683 56
BtsI GCAGTG_nn'
Cuts at: 3168 4849 5643 5663 3168
Size: 1681 794 20 4838
Fragments arranged by size:
4838 1681 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7333
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2095 2130 529
Size: 35 1531 35 5732
Fragments arranged by size:
5732 1531 35 35
DraI TTT'AAA
Cuts at: 3955 4189 4637 3955
Size: 234 448 6651
Fragments arranged by size:
6651 448 234
DraIII CAC_nnn'GTG
Cuts at: 2339 2685 2339
Size: 346 6987
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6340 809
Size: 507 5024 1802
Fragments arranged by size:
5024 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2088 2381 2406
Size: 54 1425 50 275 18 258 293 25
Cuts at: 2406 2883 3069 4780 5669 7298 8
Size: 477 186 1711 889 1629 43
Fragments arranged by size:
1711 1629 1425 889 477 293 275 258
186 54 50 43 25 18
EagI C'GGCC_G
Cuts at: 1537 1812 2088 4780 1537
Size: 275 276 2692 4090
Fragments arranged by size:
4090 2692 276 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2550 3771 4224 5263 1353
Size: 1197 1221 453 1039 3423
Fragments arranged by size:
3423 1221 1197 1039 453
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4570 5920 6294 6440 387
Size: 1064 138 2981 1350 374 146 1280
Fragments arranged by size:
2981 1350 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2072 2099 2880 3345 3412 3459 3674
Size: 393 440 27 781 465 67 47 215
Cuts at: 3674 3728 3752 4205 4440 4569 4640 5396 5993
Size: 54 24 453 235 129 71 756 597
Cuts at: 5993 6780 7048 1239
Size: 787 268 1524
Fragments arranged by size:
1524 787 781 756 597 465 453 440
393 268 235 215 129 71 67 54
47 27 24
EcoO109I rG'GnC_Cy
Cuts at: 2774 2774
Size: 7333
EcoRI G'AATT_C
Cuts at: 2846 3929 4642 5020 2846
Size: 1083 713 378 5159
Fragments arranged by size:
5159 1083 713 378
EcoRV GAT'ATC
Cuts at: 2218 2218
Size: 7333
FspI TGC'GCA
Cuts at: 5839 5839
Size: 7333
HaeII r_GCGC'y
Cuts at: 1678 6480 1678
Size: 4802 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2023 2055 2259 2291 2624
Size: 32 135 32 451 32 204 32 333
Cuts at: 2624 2656 3364 3396 3731 3763 5974 6006 6048
Size: 32 708 32 335 32 2211 32 42
Cuts at: 6048 6080 1373
Size: 32 2626
Fragments arranged by size:
2626 2211 708 451 335 333 204 135
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2451 4545 4677 4923 669
Size: 988 281 513 2094 132 246 3079
Fragments arranged by size:
3079 2094 988 513 281 246 132
HindIII A'AGCT_T
Cuts at: 748 2892 748
Size: 2144 5189
HpaI GTT'AAC
Cuts at: 4545 4923 4545
Size: 378 6955
KpnI G_GTAC'C
Cuts at: 3151 4658 3151
Size: 1507 5826
MfeI C'AATT_G
Cuts at: 4932 4932
Size: 7333
MluI A'CGCG_T
Cuts at: 7209 7209
Size: 7333
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4077 6447 6631 1305
Size: 2772 2370 184 2007
Fragments arranged by size:
2772 2370 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2383 2408 2885 3071 10
Size: 54 1425 343 551 25 477 186 4272
Fragments arranged by size:
4272 1425 551 477 343 186 54 25
MslI CAynn'nnrTG
Cuts at: 518 1166 3798 3908 4359 4437 5291 5650 5809
Size: 648 2632 110 451 78 854 359 159
Cuts at: 5809 518
Size: 2042
Fragments arranged by size:
2632 2042 854 648 451 359 159 110
78
MspA1I CmG'CkG
Cuts at: 1606 2174 2401 3287 3860 4013 4087 4749 5426
Size: 568 227 886 573 153 74 662 677
Cuts at: 5426 6574 6819 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 886 677 662 573 568 245
227 153 74
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5842
Fragments arranged by size:
5842 939 552
NdeI CA'TA_TG
Cuts at: 387 3589 387
Size: 3202 4131
NheI G'CTAG_C
Cuts at: 1052 2578 1052
Size: 1526 5807
NotI GC'GGCC_GC
Cuts at: 4780 4780
Size: 7333
NspI r_CATG'y
Cuts at: 1132 1229 3104 4691 6236 1132
Size: 97 1875 1587 1545 2229
Fragments arranged by size:
2229 1875 1587 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7333
PciI A'CATG_T
Cuts at: 3100 6232 3100
Size: 3132 4201
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7333
PfoI T'CCnGG_A
Cuts at: 2076 2143 2076
Size: 67 7266
PmeI GTTT'AAAC
Cuts at: 4637 4637
Size: 7333
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5402 5434 6915 6947 5402
Size: 32 1481 32 5788
Fragments arranged by size:
5788 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2774 2774
Size: 7333
PshAI GACnn'nnGTC
Cuts at: 1613 3659 1613
Size: 2046 5287
PsiI TTA'TAA
Cuts at: 4903 4903
Size: 7333
PstI C_TGCA'G
Cuts at: 830 3460 4685 830
Size: 2630 1225 3478
Fragments arranged by size:
3478 2630 1225
PvuI CG_AT'CG
Cuts at: 2001 2047 2001
Size: 46 7287
PvuII CAG'CTG
Cuts at: 2174 2401 3287 3860 4013 4087 2174
Size: 227 886 573 153 74 5420
Fragments arranged by size:
5420 886 573 227 153 74
SalI G'TCGA_C
Cuts at: 1936 2449 4675 1936
Size: 513 2226 4594
Fragments arranged by size:
4594 2226 513
SapI GCTCTTCn'nnn_
Cuts at: 2550 2550
Size: 7333
SbfI CC_TGCA'GG
Cuts at: 4685 4685
Size: 7333
ScaI AGT'ACT
Cuts at: 5581 7293 5581
Size: 1712 5621
SexAI A'CCwGG_T
Cuts at: 3996 3996
Size: 7333
SfcI C'TryA_G
Cuts at: 826 1045 2960 3297 3362 3456 3543 4681 5816
Size: 219 1915 337 65 94 87 1138 1135
Cuts at: 5816 6497 6688 826
Size: 681 191 1471
Fragments arranged by size:
1915 1471 1138 1135 681 337 219 191
94 87 65
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7333
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7333
SmaI CCC'GGG
Cuts at: 1872 4660 1872
Size: 2788 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2024 3935 5437 6338 6600 820
Size: 197 926 81 1911 1502 901 262 1553
Fragments arranged by size:
1911 1553 1502 926 901 262 197 81
SnaBI TAC'GTA
Cuts at: 493 5124 493
Size: 4631 2702
SpeI A'CTAG_T
Cuts at: 152 3217 3907 152
Size: 3065 690 3578
Fragments arranged by size:
3578 3065 690
SphI G_CATG'C
Cuts at: 1132 4691 1132
Size: 3559 3774
SspI AAT'ATT
Cuts at: 5 52 5257 5
Size: 47 5205 2081
Fragments arranged by size:
5205 2081 47
StuI AGG'CCT
Cuts at: 1517 3950 4229 1517
Size: 2433 279 4621
Fragments arranged by size:
4621 2433 279
StyI C'CwwG_G
Cuts at: 513 1065 2004 3624 4772 513
Size: 552 939 1620 1148 3074
Fragments arranged by size:
3074 1620 1148 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3911 5360 5377 5530 5715 6160 325
Size: 1255 2331 1449 17 153 185 445 1498
Fragments arranged by size:
2331 1498 1449 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2754 3874 3939
Size: 274 80 33 51 1443 777 1120 65
Cuts at: 3939 4481 4501 5579 7291 96
Size: 542 20 1078 1712 138
Fragments arranged by size:
1712 1443 1120 1078 777 542 274 138
80 65 51 33 20
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2671 2703 3090 3122 7293 7325 2671
Size: 32 387 32 4171 32 2679
Fragments arranged by size:
4171 2679 387 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2035 3092 1316
Size: 719 1057 5557
Fragments arranged by size:
5557 1057 719
XbaI T'CTAG_A
Cuts at: 4669 4669
Size: 7333
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2113 2376 3857 2113
Size: 263 1481 5589
Fragments arranged by size:
5589 1481 263
XhoI C'TCGA_G
Cuts at: 1943 2024 1943
Size: 81 7252
XmaI C'CCGG_G
Cuts at: 1870 4658 1870
Size: 2788 4545
XmnI GAAnn'nnTTC
Cuts at: 2391 4392 5462 2391
Size: 2001 1070 4262
Fragments arranged by size:
4262 2001 1070
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5141 276
Size: 53 83 186 936 3607 2468
Fragments arranged by size:
3607 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI
SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BbeI BfrBI BplI
BsiWI BsmBI BstZ17I EcoICRI EcoNI FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI
PspOMI PspXI PsrI RsrII SacI SacII SanDI SfiI
SfoI SrfI SwaI