Restriction map for
pFN21AB3411
(Circular) (Six-base) MAPSORT of: pFN21AB3411.seq Check: 6038 from: 1 to: 7402
pFN21AB3411 7402 bp
With 182 enzymes: SgfI *
February 27, 2009 14:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3450 3588 3450
Size: 138 7264
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5212 278
Size: 53 83 186 936 3676 2468
Fragments arranged by size:
3676 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4416 4495 4745 1937
Size: 2479 79 250 4594
Fragments arranged by size:
4594 2479 250 79
Acc65I G'GTAC_C
Cuts at: 4041 4723 4041
Size: 682 6720
AclI AA'CG_TT
Cuts at: 1563 5529 5902 1563
Size: 3966 373 3063
Fragments arranged by size:
3966 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2450 2567 2651 2708 3734 3897 4088 4176
Size: 818 117 84 57 1026 163 191 88
Cuts at: 4176 5465 6849 7117 1632
Size: 1289 1384 268 1917
Fragments arranged by size:
1917 1384 1289 1026 818 268 191 163
117 88 84 57
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7205
AflIII A'CryG_T
Cuts at: 1203 2952 4419 6301 7278 1203
Size: 1749 1467 1882 977 1327
Fragments arranged by size:
1882 1749 1467 1327 977
AgeI A'CCGG_T
Cuts at: 6233 6233
Size: 7402
AhdI GACnn_n'nnGTC
Cuts at: 6131 6131
Size: 7402
AleI CACnn'nnGTG
Cuts at: 1166 2843 3072 4226 4583 1166
Size: 1677 229 1154 357 3985
Fragments arranged by size:
3985 1677 1154 357 229
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4472 1184
Size: 3288 4114
AlwNI CAG_nnn'CTG
Cuts at: 2720 3719 4336 5145 6717 2720
Size: 999 617 809 1572 3405
Fragments arranged by size:
3405 1572 999 809 617
ApaI G_GGCC'C
Cuts at: 2382 3185 4646 2382
Size: 803 1461 5138
Fragments arranged by size:
5138 1461 803
ApaLI G'TGCA_C
Cuts at: 3741 4697 5459 6615 3741
Size: 956 762 1156 4528
Fragments arranged by size:
4528 1156 956 762
ApoI r'AATT_y
Cuts at: 1493 4711 4937 5089 7198 1493
Size: 3218 226 152 2109 1697
Fragments arranged by size:
3218 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5956 160
Size: 872 4924 1606
Fragments arranged by size:
4924 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7402
AvaI C'yCGr_G
Cuts at: 1870 1943 3199 3333 3378 4560 4727 1870
Size: 73 1256 134 45 1182 167 4545
Fragments arranged by size:
4545 1256 1182 167 134 73 45
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4025 4058 4025
Size: 33 7369
BamHI G'GATC_C
Cuts at: 4732 5099 4732
Size: 367 7035
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2204 3235 4041 4680 4723
Size: 325 217 627 417 1031 806 639 43
Cuts at: 4723 6178 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1031 806 639 627 417 325
217 43
BanII G_rGCy'C
Cuts at: 1391 2382 3185 3334 3385 3763 3835 4107 4646
Size: 991 803 149 51 378 72 272 539
Cuts at: 4646 4671 4721 1391
Size: 25 50 4072
Fragments arranged by size:
4072 991 803 539 378 272 149 72
51 50 25
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3000 4238 4599 928
Size: 971 1101 1238 361 3731
Fragments arranged by size:
3731 1238 1101 971 361
BbvCI CC'TCA_GC
Cuts at: 4544 4544
Size: 7402
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3314 3348 5593 5627 1903
Size: 34 1377 34 2245 34 3678
Fragments arranged by size:
3678 2245 1377 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3819 5294 6504 3819
Size: 1475 1210 4717
Fragments arranged by size:
4717 1475 1210
BclI T'GATC_A
Cuts at: 1548 3609 1548
Size: 2061 5341
BfrBI ATG'CAT
Cuts at: 3697 3697
Size: 7402
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3189 3357 6013
Size: 107 122 71 631 605 1517 168 2656
Cuts at: 6013 136
Size: 1525
Fragments arranged by size:
2656 1525 1517 631 605 168 122 107
71
BglII A'GATC_T
Cuts at: 2247 7397 2247
Size: 5150 2252
BlpI GC'TnA_GC
Cuts at: 4819 4819
Size: 7402
Bme1580I G_kGCm'C
Cuts at: 1790 2382 3185 3240 3745 3774 4115 4646 4683
Size: 592 803 55 505 29 341 531 37
Cuts at: 4683 4701 5463 6619 1790
Size: 18 762 1156 2573
Fragments arranged by size:
2573 1156 803 762 592 531 505 341
55 37 29 18
BmgBI CAC'GTC
Cuts at: 1318 2953 1318
Size: 1635 5767
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3289 4046 4437
Size: 416 53 83 399 24 1872 757 391
Cuts at: 4437 5133 6091 442
Size: 696 958 1753
Fragments arranged by size:
1872 1753 958 757 696 416 399 391
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7402
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3404 3777 3404
Size: 373 7029
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2194 2546 3135 3268 4358 4520 6062 1239
Size: 955 352 589 133 1090 162 1542 2579
Fragments arranged by size:
2579 1542 1090 955 589 352 162 133
Bpu10I CC'TnA_GC
Cuts at: 2142 3729 4544 4653 2142
Size: 1587 815 109 4891
Fragments arranged by size:
4891 1587 815 109
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5527 6392 6690 5527
Size: 865 298 6239
Fragments arranged by size:
6239 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6065 882
Size: 617 4566 2219
Fragments arranged by size:
4566 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4422 5193 493
Size: 711 3218 771 2702
Fragments arranged by size:
3218 2702 771 711
BsaBI GATnn'nnATC
Cuts at: 7396 7396
Size: 7402
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3399 3590 5209 5591
Size: 53 83 186 936 1866 191 1619 382
Cuts at: 5591 6226 275
Size: 635 1451
Fragments arranged by size:
1866 1619 1451 936 635 382 191 186
83 53
BsaWI w'CCGG_w
Cuts at: 2871 5835 6233 6507 6654 7065 2871
Size: 2964 398 274 147 411 3208
Fragments arranged by size:
3208 2964 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2724 2754 2937 2967 3932
Size: 30 165 30 1321 30 183 30 965
Cuts at: 3932 3962 4094 4124 4493 4523 1178
Size: 30 132 30 369 30 4057
Fragments arranged by size:
4057 1321 965 369 183 165 132 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2059 2958 3123 3132 3136 3953 4115 4541
Size: 873 899 165 9 4 817 162 426
Cuts at: 4541 1186
Size: 4047
Fragments arranged by size:
4047 899 873 817 426 165 162 9
4
BseYI C'CCAG_C
Cuts at: 2353 2646 3045 3239 3661 3774 3943 4286 4291
Size: 293 399 194 422 113 169 343 5
Cuts at: 4291 4374 4574 4639 4686 6605 7101 2353
Size: 83 200 65 47 1919 496 2654
Fragments arranged by size:
2654 1919 496 422 399 343 293 200
194 169 113 83 65 47 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2783 3029 3111 3464 3566 3581 3675
Size: 573 1047 246 82 353 102 15 94
Cuts at: 3675 4004 1163
Size: 329 4561
Fragments arranged by size:
4561 1047 573 353 329 246 102 94
82 15
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4852 5613 6641 1540
Size: 275 186 2851 761 1028 2301
Fragments arranged by size:
2851 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2317 3334 3727 3745 3763 4701 5463 5548 6619
Size: 1017 393 18 18 938 762 85 1071
Cuts at: 6619 2317
Size: 3100
Fragments arranged by size:
3100 1071 1017 938 762 393 85 18
18
BsiWI C'GTAC_G
Cuts at: 4602 4602
Size: 7402
BsmI GAATG_Cn'
Cuts at: 4913 5006 4913
Size: 93 7309
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2317 2382 3185 3240 3334 3385 3727
Size: 399 527 65 803 55 94 51 342
Cuts at: 3727 3745 3763 3774 3835 4107 4115 4646 4671
Size: 18 18 11 61 272 8 531 25
Cuts at: 4671 4683 4701 4721 5463 5548 6619 1391
Size: 12 18 20 742 85 1071 2174
Fragments arranged by size:
2174 1071 803 742 531 527 399 342
272 94 85 65 61 55 51 25
20 18 18 18 12 11 8
BspEI T'CCGG_A
Cuts at: 2871 7065 2871
Size: 4194 3208
BspHI T'CATG_A
Cuts at: 5291 7021 5291
Size: 1730 5672
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3450 3588 4481 4757 844
Size: 2606 138 893 276 3489
Fragments arranged by size:
3489 2606 893 276 138
BsrBI CCG'CTC
Cuts at: 1394 1823 2484 2905 3895 5289 1394
Size: 429 661 421 990 1394 3507
Fragments arranged by size:
3507 1394 990 661 429 421
BsrDI GCAATG_nn'
Cuts at: 65 1248 2348 5897 6071 65
Size: 1183 1100 3549 174 1396
Fragments arranged by size:
3549 1396 1183 1100 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6046 6233 1700
Size: 110 4236 187 2869
Fragments arranged by size:
4236 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7402
BssHII G'CGCG_C
Cuts at: 1925 3347 3413 3415 3417 1925
Size: 1422 66 2 2 5910
Fragments arranged by size:
5910 1422 66 2 2
BssSI C'ACGA_G
Cuts at: 2310 5462 6474 2310
Size: 3152 1012 3238
Fragments arranged by size:
3238 3152 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2343 794
Size: 1549 5853
BstBI TT'CG_AA
Cuts at: 6206 6206
Size: 7402
BstEII G'GTnAC_C
Cuts at: 1184 3952 3964 1184
Size: 2768 12 4622
Fragments arranged by size:
4622 2768 12
BstXI CCAn_nnnn'nTGG
Cuts at: 3074 3700 3934 3074
Size: 626 234 6542
Fragments arranged by size:
6542 626 234
BstYI r'GATC_y
Cuts at: 1972 2247 2358 2560 3853 4732 5099 5484 5501
Size: 275 111 202 1293 879 367 385 17
Cuts at: 5501 6953 7039 7051 7397 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1293 879 385 367 346 275
202 111 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 4692 4692
Size: 7402
BtgI C'CryG_G
Cuts at: 513 1065 2004 2188 3186 3231 3264 3405 3496
Size: 552 939 184 998 45 33 141 91
Cuts at: 3496 4263 4675 513
Size: 767 412 3240
Fragments arranged by size:
3240 998 939 767 552 412 184 141
91 45 33
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6663
Fragments arranged by size:
6663 683 56
BtsI GCAGTG_nn'
Cuts at: 3476 3743 4115 4918 5712 5732 3476
Size: 267 372 803 794 20 5146
Fragments arranged by size:
5146 803 794 372 267 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7402
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7367
DraI TTT'AAA
Cuts at: 4706 4706
Size: 7402
DraIII CAC_nnn'GTG
Cuts at: 2300 2312 4316 4445 2300
Size: 12 2004 129 5257
Fragments arranged by size:
5257 2004 129 12
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3398 6409 809
Size: 507 2082 3011 1802
Fragments arranged by size:
3011 2082 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3225 4849 5738
Size: 54 1425 50 275 18 1395 1624 889
Cuts at: 5738 7367 8
Size: 1629 43
Fragments arranged by size:
1629 1624 1425 1395 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4849 1537
Size: 275 3037 4090
Fragments arranged by size:
4090 3037 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2997 5332 1353
Size: 1644 2335 3423
Fragments arranged by size:
3423 2335 1644
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5989 6363 6509 387
Size: 1064 138 4400 374 146 1280
Fragments arranged by size:
4400 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3332 3761 3332
Size: 429 6973
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2194 2450 2546 2567 2651 2708 3135
Size: 393 562 256 96 21 84 57 427
Cuts at: 3135 3268 3734 3897 4088 4176 4358 4520 5465
Size: 133 466 163 191 88 182 162 945
Cuts at: 5465 6062 6849 7117 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 945 787 597 562 466 427 393
268 256 191 182 163 162 133 96
88 84 57 21
EcoNI CCTnn'n_nnAGG
Cuts at: 2504 4067 4526 2504
Size: 1563 459 5380
Fragments arranged by size:
5380 1563 459
EcoO109I rG'GnC_Cy
Cuts at: 2180 2378 2490 2497 3182 3288 3310 3550 4160
Size: 198 112 7 685 106 22 240 610
Cuts at: 4160 4483 4504 2180
Size: 323 21 5078
Fragments arranged by size:
5078 685 610 323 240 198 112 106
22 21 7
EcoRI G'AATT_C
Cuts at: 4711 5089 4711
Size: 378 7024
FspI TGC'GCA
Cuts at: 4406 4460 5908 4406
Size: 54 1448 5900
Fragments arranged by size:
5900 1448 54
HaeII r_GCGC'y
Cuts at: 1678 3279 3378 3439 3707 3720 6549 1678
Size: 1601 99 61 268 13 2829 2531
Fragments arranged by size:
2829 2531 1601 268 99 61 13
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2651 2683 4130 4136 4162
Size: 32 135 32 1079 32 1447 6 26
Cuts at: 4162 4168 4493 4525 6043 6075 6117 6149 1373
Size: 6 325 32 1518 32 42 32 2626
Fragments arranged by size:
2626 1518 1447 1079 325 135 42 32
32 32 32 32 32 26 6 6
HincII GTy'rAC
Cuts at: 669 1657 1938 3565 4746 4992 669
Size: 988 281 1627 1181 246 3079
Fragments arranged by size:
3079 1627 1181 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7402
HpaI GTT'AAC
Cuts at: 4992 4992
Size: 7402
KpnI G_GTAC'C
Cuts at: 4045 4727 4045
Size: 682 6720
MfeI C'AATT_G
Cuts at: 5001 5001
Size: 7402
MluI A'CGCG_T
Cuts at: 7278 7278
Size: 7402
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6516 6700 1305
Size: 5211 184 2007
Fragments arranged by size:
5211 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5580
Fragments arranged by size:
5580 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2843 3072 3932 4226 4583 5360 5719
Size: 648 1677 229 860 294 357 777 359
Cuts at: 5719 5878 518
Size: 159 2042
Fragments arranged by size:
2042 1677 860 777 648 359 357 294
229 159
MspA1I CmG'CkG
Cuts at: 1606 2108 2476 2518 3013 3266 3407 3778 4031
Size: 502 368 42 495 253 141 371 253
Cuts at: 4031 4345 4374 4818 5495 6643 6888 1606
Size: 314 29 444 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 502 495 444 371 368
314 253 253 245 141 42 29
NcoI C'CATG_G
Cuts at: 513 1065 2004 2188 3186 4263 513
Size: 552 939 184 998 1077 3652
Fragments arranged by size:
3652 1077 998 939 552 184
NdeI CA'TA_TG
Cuts at: 387 3786 387
Size: 3399 4003
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7402
NotI GC'GGCC_GC
Cuts at: 4849 4849
Size: 7402
NsiI A_TGCA'T
Cuts at: 3699 3699
Size: 7402
NspI r_CATG'y
Cuts at: 1132 1229 2426 4760 6305 1132
Size: 97 1197 2334 1545 2229
Fragments arranged by size:
2334 2229 1545 1197 97
PasI CC'CwG_GG
Cuts at: 1348 2494 4366 1348
Size: 1146 1872 4384
Fragments arranged by size:
4384 1872 1146
PciI A'CATG_T
Cuts at: 6301 6301
Size: 7402
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2329 4618 1108
Size: 1221 2289 3892
Fragments arranged by size:
3892 2289 1221
PfoI T'CCnGG_A
Cuts at: 3284 3284
Size: 7402
PmeI GTTT'AAAC
Cuts at: 4706 4706
Size: 7402
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2771 2803 5471 5503 6984 7016 2771
Size: 32 2668 32 1481 32 3157
Fragments arranged by size:
3157 2668 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3288 3550 4483 3288
Size: 262 933 6207
Fragments arranged by size:
6207 933 262
PshAI GACnn'nnGTC
Cuts at: 1613 3509 1613
Size: 1896 5506
PsiI TTA'TAA
Cuts at: 4972 4972
Size: 7402
PspOMI G'GGCC_C
Cuts at: 2378 3181 4642 2378
Size: 803 1461 5138
Fragments arranged by size:
5138 1461 803
PstI C_TGCA'G
Cuts at: 830 3327 4332 4754 830
Size: 2497 1005 422 3478
Fragments arranged by size:
3478 2497 1005 422
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7402
PvuII CAG'CTG
Cuts at: 2108 3778 4031 4345 4374 2108
Size: 1670 253 314 29 5136
Fragments arranged by size:
5136 1670 314 253 29
RsrII CG'GwC_CG
Cuts at: 3826 3826
Size: 7402
SacI G_AGCT'C
Cuts at: 3334 3763 3334
Size: 429 6973
SacII CC_GC'GG
Cuts at: 3267 3408 3267
Size: 141 7261
SalI G'TCGA_C
Cuts at: 1936 4744 1936
Size: 2808 4594
SbfI CC_TGCA'GG
Cuts at: 4754 4754
Size: 7402
ScaI AGT'ACT
Cuts at: 5650 7362 5650
Size: 1712 5690
SexAI A'CCwGG_T
Cuts at: 2121 2286 2121
Size: 165 7237
SfcI C'TryA_G
Cuts at: 826 1045 3157 3323 4328 4750 5885 6566 6757
Size: 219 2112 166 1005 422 1135 681 191
Cuts at: 6757 826
Size: 1471
Fragments arranged by size:
2112 1471 1135 1005 681 422 219 191
166
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7402
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7402
SmaI CCC'GGG
Cuts at: 1872 3201 4729 1872
Size: 1329 1528 4545
Fragments arranged by size:
4545 1528 1329
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3333 5506 6407 6669 820
Size: 197 926 1390 2173 901 262 1553
Fragments arranged by size:
2173 1553 1390 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5193 493
Size: 4700 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7402
SphI G_CATG'C
Cuts at: 1132 4760 1132
Size: 3628 3774
SspI AAT'ATT
Cuts at: 5 52 5326 5
Size: 47 5274 2081
Fragments arranged by size:
5274 2081 47
StuI AGG'CCT
Cuts at: 1517 2819 3029 1517
Size: 1302 210 5890
Fragments arranged by size:
5890 1302 210
StyI C'CwwG_G
Cuts at: 513 1065 2004 2085 2188 2382 3186 4224 4263
Size: 552 939 81 103 194 804 1038 39
Cuts at: 4263 4841 513
Size: 578 3074
Fragments arranged by size:
3074 1038 939 804 578 552 194 103
81 39
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2402 3062 3971 4456 5429 5446 5599
Size: 1255 822 660 909 485 973 17 153
Cuts at: 5599 5784 6229 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 973 909 822 660 485 445
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3339 3814 5648
Size: 274 80 33 51 1443 1362 475 1834
Cuts at: 5648 7360 96
Size: 1712 138
Fragments arranged by size:
1834 1712 1443 1362 475 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2937 2969 4094 4126 7362 7394 2937
Size: 32 1125 32 3236 32 2945
Fragments arranged by size:
3236 2945 1125 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7402
XbaI T'CTAG_A
Cuts at: 4738 4738
Size: 7402
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2278 2278
Size: 7402
XhoI C'TCGA_G
Cuts at: 1943 3333 1943
Size: 1390 6012
XmaI C'CCGG_G
Cuts at: 1870 3199 4727 1870
Size: 1329 1528 4545
Fragments arranged by size:
4545 1528 1329
XmnI GAAnn'nnTTC
Cuts at: 5531 5531
Size: 7402
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5210 276
Size: 53 83 186 936 3676 2468
Fragments arranged by size:
3676 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacI
SacII SalI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BbeI BsmBI BstZ17I EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PmlI PspXI PsrI SanDI SapI SfiI SfoI
SrfI SwaI