Restriction map for
pFN21AB3410
(Circular) (Six-base) MAPSORT of: pFN21AB3410.seq Check: 5226 from: 1 to: 6994
pFN21AB3410 6994 bp
With 182 enzymes: SgfI *
February 27, 2009 14:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2050 2617 2050
Size: 567 6427
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4804 278
Size: 53 83 186 936 3268 2468
Fragments arranged by size:
3268 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4337 1937
Size: 2400 4594
Acc65I G'GTAC_C
Cuts at: 4315 4315
Size: 6994
AclI AA'CG_TT
Cuts at: 1563 5121 5494 1563
Size: 3558 373 3063
Fragments arranged by size:
3558 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2273 2414 2510 3050 3235 3287 5057 6441
Size: 641 141 96 540 185 52 1770 1384
Cuts at: 6441 6709 1632
Size: 268 1917
Fragments arranged by size:
1917 1770 1384 641 540 268 185 141
96 52
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6797
AflIII A'CryG_T
Cuts at: 1203 3258 4119 5893 6870 1203
Size: 2055 861 1774 977 1327
Fragments arranged by size:
2055 1774 1327 977 861
AgeI A'CCGG_T
Cuts at: 5825 5825
Size: 6994
AhdI GACnn_n'nnGTC
Cuts at: 2413 5723 2413
Size: 3310 3684
AleI CACnn'nnGTG
Cuts at: 1166 3965 1166
Size: 2799 4195
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6994
AlwNI CAG_nnn'CTG
Cuts at: 2032 2065 2185 2206 2359 2668 2722 2785 2911
Size: 33 120 21 153 309 54 63 126
Cuts at: 2911 3028 3520 4049 4737 6309 2032
Size: 117 492 529 688 1572 2717
Fragments arranged by size:
2717 1572 688 529 492 309 153 126
120 117 63 54 33 21
ApaI G_GGCC'C
Cuts at: 2805 2805
Size: 6994
ApaLI G'TGCA_C
Cuts at: 3089 5051 6207 3089
Size: 1962 1156 3876
Fragments arranged by size:
3876 1962 1156
ApoI r'AATT_y
Cuts at: 1493 3452 4163 4303 4529 4681 6790 1493
Size: 1959 711 140 226 152 2109 1697
Fragments arranged by size:
2109 1959 1697 711 226 152 140
AseI AT'TA_AT
Cuts at: 160 1032 5548 160
Size: 872 4516 1606
Fragments arranged by size:
4516 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6994
AvaI C'yCGr_G
Cuts at: 1870 1943 2807 4319 1870
Size: 73 864 1512 4545
Fragments arranged by size:
4545 1512 864 73
AvrII C'CTAG_G
Cuts at: 3694 3694
Size: 6994
BamHI G'GATC_C
Cuts at: 2016 2397 2547 4324 4691 2016
Size: 381 150 1777 367 4319
Fragments arranged by size:
4319 1777 381 367 150
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3233 3391 4315 5770 618
Size: 325 217 627 1446 158 924 1455 1842
Fragments arranged by size:
1842 1455 1446 924 627 325 217 158
BanII G_rGCy'C
Cuts at: 1391 2170 2585 2805 4313 1391
Size: 779 415 220 1508 4072
Fragments arranged by size:
4072 1508 779 415 220
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2714 3038 3937 928
Size: 971 815 324 899 3985
Fragments arranged by size:
3985 971 899 815 324
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3778 3812 5185 5219 1903
Size: 34 1841 34 1373 34 3678
Fragments arranged by size:
3678 1841 1373 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4886 6096 4886
Size: 1210 5784
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6994
BfrBI ATG'CAT
Cuts at: 4037 4037
Size: 6994
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2570 5605 136
Size: 107 122 71 631 605 898 3035 1525
Fragments arranged by size:
3035 1525 898 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6989 6989
Size: 6994
BlpI GC'TnA_GC
Cuts at: 2579 2892 4411 2579
Size: 313 1519 5162
Fragments arranged by size:
5162 1519 313
Bme1580I G_kGCm'C
Cuts at: 1790 2271 2805 3093 3236 3247 4103 5055 6211
Size: 481 534 288 143 11 856 952 1156
Cuts at: 6211 1790
Size: 2573
Fragments arranged by size:
2573 1156 952 856 534 481 288 143
11
BmgBI CAC'GTC
Cuts at: 1318 2824 1318
Size: 1506 5488
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2104 2921 3574
Size: 416 53 83 399 24 687 817 653
Cuts at: 3574 4725 5683 442
Size: 1151 958 1753
Fragments arranged by size:
1753 1151 958 817 687 653 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6994
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3964 4304 3964
Size: 340 6654
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2207 2270 2333 2600 2657 3566 5654 1239
Size: 968 63 63 267 57 909 2088 2579
Fragments arranged by size:
2579 2088 968 909 267 63 63 57
Bpu10I CC'TnA_GC
Cuts at: 3899 3899
Size: 6994
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5119 5984 6282 5119
Size: 865 298 5831
Fragments arranged by size:
5831 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4172 5657 882
Size: 617 2673 1485 2219
Fragments arranged by size:
2673 2219 1485 617
BsaAI yAC'GTr
Cuts at: 493 1204 4068 4201 4785 493
Size: 711 2864 133 584 2702
Fragments arranged by size:
2864 2702 711 584 133
BsaBI GATnn'nnATC
Cuts at: 6988 6988
Size: 6994
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2409 4801 5183 5818
Size: 53 83 186 936 876 2392 382 635
Cuts at: 5818 275
Size: 1451
Fragments arranged by size:
2392 1451 936 876 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5427 5825 6099 6246 6657 5427
Size: 398 274 147 411 5764
Fragments arranged by size:
5764 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2630 2660 1178
Size: 30 165 30 1227 30 5512
Fragments arranged by size:
5512 1227 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2465 2948 3009 3329 1186
Size: 1279 483 61 320 4851
Fragments arranged by size:
4851 1279 483 320 61
BseYI C'CCAG_C
Cuts at: 2025 2178 2263 2393 2565 2574 3889 4049 4090
Size: 153 85 130 172 9 1315 160 41
Cuts at: 4090 6197 6693 2025
Size: 2107 496 2326
Fragments arranged by size:
2326 2107 1315 496 172 160 153 130
85 41 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2219 3199 3318 3488 1163
Size: 573 483 980 119 170 4669
Fragments arranged by size:
4669 980 573 483 170 119
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4444 5205 6233 1540
Size: 275 186 2443 761 1028 2301
Fragments arranged by size:
2443 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2226 2898 3093 5055 5140 6211 2226
Size: 672 195 1962 85 1071 3009
Fragments arranged by size:
3009 1962 1071 672 195 85
BsiWI C'GTAC_G
Cuts at: 3058 3058
Size: 6994
BsmI GAATG_Cn'
Cuts at: 4039 4505 4598 4039
Size: 466 93 6435
Fragments arranged by size:
6435 466 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2034 2600 2034
Size: 566 6428
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2170 2226 2271 2585 2805 2898 3093
Size: 399 380 56 45 314 220 93 195
Cuts at: 3093 3236 3247 4103 4313 5055 5140 6211 1391
Size: 143 11 856 210 742 85 1071 2174
Fragments arranged by size:
2174 1071 856 742 399 380 314 220
210 195 143 93 85 56 45 11
BspEI T'CCGG_A
Cuts at: 6657 6657
Size: 6994
BspHI T'CATG_A
Cuts at: 4883 6613 4883
Size: 1730 5264
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2050 2617 3735 4349 844
Size: 1206 567 1118 614 3489
Fragments arranged by size:
3489 1206 1118 614 567
BsrBI CCG'CTC
Cuts at: 1394 1823 4881 1394
Size: 429 3058 3507
Fragments arranged by size:
3507 3058 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3367 5489 5663 65
Size: 1183 2119 2122 174 1396
Fragments arranged by size:
2122 2119 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2871 3288 3885 4259 5638 5825 1700
Size: 110 1061 417 597 374 1379 187 2869
Fragments arranged by size:
2869 1379 1061 597 417 374 187 110
BsrGI T'GTAC_A
Cuts at: 96 2343 96
Size: 2247 4747
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6994
BssSI C'ACGA_G
Cuts at: 3500 5054 6066 3500
Size: 1554 1012 4428
Fragments arranged by size:
4428 1554 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2206 2722 3177 794
Size: 1412 516 455 4611
Fragments arranged by size:
4611 1412 516 455
BstBI TT'CG_AA
Cuts at: 5798 5798
Size: 6994
BstEII G'GTnAC_C
Cuts at: 1184 2986 1184
Size: 1802 5192
BstXI CCAn_nnnn'nTGG
Cuts at: 2146 2746 4072 4189 4201 2146
Size: 600 1326 117 12 4939
Fragments arranged by size:
4939 1326 600 117 12
BstYI r'GATC_y
Cuts at: 1972 2016 2397 2547 2880 3189 4324 4691 5076
Size: 44 381 150 333 309 1135 367 385
Cuts at: 5076 5093 6545 6631 6643 6989 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1135 385 381 367 346 333
309 150 86 44 17 12
Bsu36I CC'TnA_GG
Cuts at: 2543 3021 2543
Size: 478 6516
BtgI C'CryG_G
Cuts at: 513 1065 2004 2129 3228 4182 513
Size: 552 939 125 1099 954 3325
Fragments arranged by size:
3325 1099 954 939 552 125
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3781 487
Size: 683 56 2555 3700
Fragments arranged by size:
3700 2555 683 56
BtsI GCAGTG_nn'
Cuts at: 2666 3187 3632 4510 5304 5324 2666
Size: 521 445 878 794 20 4336
Fragments arranged by size:
4336 878 794 521 445 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6994
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6959
DraI TTT'AAA
Cuts at: 4298 4298
Size: 6994
DraIII CAC_nnn'GTG
Cuts at: 2230 2230
Size: 6994
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6001 809
Size: 507 4685 1802
Fragments arranged by size:
4685 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2071 2691 2787
Size: 54 1425 50 275 18 241 620 96
Cuts at: 2787 2850 2943 3422 4191 4441 5330 6959 8
Size: 63 93 479 769 250 889 1629 43
Fragments arranged by size:
1629 1425 889 769 620 479 275 250
241 96 93 63 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4441 1537
Size: 275 2629 4090
Fragments arranged by size:
4090 2629 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3263 4924 1353
Size: 1910 1661 3423
Fragments arranged by size:
3423 1910 1661
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2234 5581 5955 6101 387
Size: 1064 138 645 3347 374 146 1280
Fragments arranged by size:
3347 1280 1064 645 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2207 2270 2273 2333 2414 2510 2600
Size: 393 575 63 3 60 81 96 90
Cuts at: 2600 2657 3050 3235 3287 3566 5057 5654 6441
Size: 57 393 185 52 279 1491 597 787
Cuts at: 6441 6709 1239
Size: 268 1524
Fragments arranged by size:
1524 1491 787 597 575 393 393 279
268 185 96 90 81 63 60 57
52 3
EcoNI CCTnn'n_nnAGG
Cuts at: 2198 3488 2198
Size: 1290 5704
EcoO109I rG'GnC_Cy
Cuts at: 2191 2802 3025 3481 3750 3873 3895 4081 2191
Size: 611 223 456 269 123 22 186 5104
Fragments arranged by size:
5104 611 456 269 223 186 123 22
EcoRI G'AATT_C
Cuts at: 4163 4303 4681 4163
Size: 140 378 6476
Fragments arranged by size:
6476 378 140
FspI TGC'GCA
Cuts at: 5500 5500
Size: 6994
HaeII r_GCGC'y
Cuts at: 1678 3443 4065 6141 1678
Size: 1765 622 2076 2531
Fragments arranged by size:
2531 2076 1765 622
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2399 2431 2539 2571 2960
Size: 32 135 32 827 32 108 32 389
Cuts at: 2960 2992 5635 5667 5709 5741 1373
Size: 32 2643 32 42 32 2626
Fragments arranged by size:
2643 2626 827 389 135 108 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2407 2953 4338 4584 669
Size: 988 281 469 546 1385 246 3079
Fragments arranged by size:
3079 1385 988 546 469 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6994
HpaI GTT'AAC
Cuts at: 4584 4584
Size: 6994
KpnI G_GTAC'C
Cuts at: 4319 4319
Size: 6994
MfeI C'AATT_G
Cuts at: 2377 4593 2377
Size: 2216 4778
MluI A'CGCG_T
Cuts at: 6870 6870
Size: 6994
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6108 6292 1305
Size: 4803 184 2007
Fragments arranged by size:
4803 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4193 10
Size: 54 1425 343 2361 2811
Fragments arranged by size:
2811 2361 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3678 3914 3965 4070 4187 4952 5311
Size: 648 2512 236 51 105 117 765 359
Cuts at: 5311 5470 518
Size: 159 2042
Fragments arranged by size:
2512 2042 765 648 359 236 159 117
105 51
MspA1I CmG'CkG
Cuts at: 1606 2032 2304 2325 2569 2578 2719 2785 2911
Size: 426 272 21 244 9 141 66 126
Cuts at: 2911 3430 4094 4410 5087 6235 6480 1606
Size: 519 664 316 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 664 519 426 316 272
245 244 141 126 66 21 9
NcoI C'CATG_G
Cuts at: 513 1065 2004 2129 4182 513
Size: 552 939 125 2053 3325
Fragments arranged by size:
3325 2053 939 552 125
NdeI CA'TA_TG
Cuts at: 387 2296 387
Size: 1909 5085
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6994
NotI GC'GGCC_GC
Cuts at: 4441 4441
Size: 6994
NsiI A_TGCA'T
Cuts at: 4039 4039
Size: 6994
NspI r_CATG'y
Cuts at: 1132 1229 3262 4123 4352 5897 1132
Size: 97 2033 861 229 1545 2229
Fragments arranged by size:
2229 2033 1545 861 229 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6994
PciI A'CATG_T
Cuts at: 3258 4119 5893 3258
Size: 861 1774 4359
Fragments arranged by size:
4359 1774 861
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2185 3801 1108
Size: 1077 1616 4301
Fragments arranged by size:
4301 1616 1077
PfoI T'CCnGG_A
Cuts at: 2184 2745 2184
Size: 561 6433
PmeI GTTT'AAAC
Cuts at: 4298 4298
Size: 6994
PmlI CAC'GTG
Cuts at: 4201 4201
Size: 6994
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3305 3329 3337 3356 3361 3388 5063 5095 6576
Size: 24 8 19 5 27 1675 32 1481
Cuts at: 6576 6608 3305
Size: 32 3691
Fragments arranged by size:
3691 1675 1481 32 32 27 24 19
8 5
PpuMI rG'GwC_Cy
Cuts at: 3025 3750 3873 3895 3025
Size: 725 123 22 6124
Fragments arranged by size:
6124 725 123 22
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6994
PsiI TTA'TAA
Cuts at: 4564 4564
Size: 6994
PspOMI G'GGCC_C
Cuts at: 2801 2801
Size: 6994
PstI C_TGCA'G
Cuts at: 830 2037 2211 2502 2673 2718 4045 4346 830
Size: 1207 174 291 171 45 1327 301 3478
Fragments arranged by size:
3478 1327 1207 301 291 174 171 45
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6994
PvuII CAG'CTG
Cuts at: 2032 2569 2578 2719 2785 2911 3430 4094 2032
Size: 537 9 141 66 126 519 664 4932
Fragments arranged by size:
4932 664 537 519 141 126 66 9
SalI G'TCGA_C
Cuts at: 1936 4336 1936
Size: 2400 4594
SbfI CC_TGCA'GG
Cuts at: 4346 4346
Size: 6994
ScaI AGT'ACT
Cuts at: 2516 3956 5242 6954 2516
Size: 1440 1286 1712 2556
Fragments arranged by size:
2556 1712 1440 1286
SexAI A'CCwGG_T
Cuts at: 2226 2226
Size: 6994
SfcI C'TryA_G
Cuts at: 826 1045 2033 2207 2498 2669 2714 2828 3553
Size: 219 988 174 291 171 45 114 725
Cuts at: 3553 4041 4342 5477 6158 6349 826
Size: 488 301 1135 681 191 1471
Fragments arranged by size:
1471 1135 988 725 681 488 301 291
219 191 174 171 114 45
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6994
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6994
SmaI CCC'GGG
Cuts at: 1872 4321 1872
Size: 2449 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5098 5999 6261 820
Size: 197 926 3155 901 262 1553
Fragments arranged by size:
3155 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4785 493
Size: 4292 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6994
SphI G_CATG'C
Cuts at: 1132 4352 1132
Size: 3220 3774
SspI AAT'ATT
Cuts at: 5 52 4918 5
Size: 47 4866 2081
Fragments arranged by size:
4866 2081 47
StuI AGG'CCT
Cuts at: 1517 2642 1517
Size: 1125 5869
StyI C'CwwG_G
Cuts at: 513 1065 2004 2129 3489 3694 3933 4182 4433
Size: 552 939 125 1360 205 239 249 251
Cuts at: 4433 513
Size: 3074
Fragments arranged by size:
3074 1360 939 552 251 249 239 205
125
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2192 5021 5038 5191 5376 5821 325
Size: 1255 612 2829 17 153 185 445 1498
Fragments arranged by size:
2829 1498 1255 612 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2343 2514 3954
Size: 274 80 33 51 1443 366 171 1440
Cuts at: 3954 4144 5240 6952 96
Size: 190 1096 1712 138
Fragments arranged by size:
1712 1443 1440 1096 366 274 190 171
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3265 3297 6954 6986 3265
Size: 32 3657 32 3273
Fragments arranged by size:
3657 3273 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6994
XbaI T'CTAG_A
Cuts at: 4330 4330
Size: 6994
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2182 3680 3874 2182
Size: 1498 194 5302
Fragments arranged by size:
5302 1498 194
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6994
XmaI C'CCGG_G
Cuts at: 1870 4319 1870
Size: 2449 4545
XmnI GAAnn'nnTTC
Cuts at: 3521 3845 5123 3521
Size: 324 1278 5392
Fragments arranged by size:
5392 1278 324
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4802 276
Size: 53 83 186 936 3268 2468
Fragments arranged by size:
3268 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI BaeI BbeI BbvCI BstZ17I EcoICRI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PspXI PsrI RsrII SacI SacII SanDI
SapI SfiI SfoI SrfI SwaI