Restriction map for
pFN21AB3406
(Circular) (Six-base) MAPSORT of: pFN21AB3406.seq Check: 4870 from: 1 to: 5806
pFN21AB3406 5806 bp
With 182 enzymes: SgfI *
February 27, 2009 14:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3616 278
Size: 53 83 186 936 2080 2468
Fragments arranged by size:
2468 2080 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3149 1937
Size: 1212 4594
Acc65I G'GTAC_C
Cuts at: 3127 3127
Size: 5806
AclI AA'CG_TT
Cuts at: 1563 3933 4306 1563
Size: 2370 373 3063
Fragments arranged by size:
3063 2370 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3869 5253 5521 1632
Size: 2237 1384 268 1917
Fragments arranged by size:
2237 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 2096 820
Size: 197 1079 4530
Fragments arranged by size:
4530 1079 197
AflIII A'CryG_T
Cuts at: 1203 4705 5682 1203
Size: 3502 977 1327
Fragments arranged by size:
3502 1327 977
AgeI A'CCGG_T
Cuts at: 4637 4637
Size: 5806
AhdI GACnn_n'nnGTC
Cuts at: 4535 4535
Size: 5806
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5806
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5806
AlwNI CAG_nnn'CTG
Cuts at: 2350 3549 5121 2350
Size: 1199 1572 3035
Fragments arranged by size:
3035 1572 1199
ApaLI G'TGCA_C
Cuts at: 3863 5019 3863
Size: 1156 4650
ApoI r'AATT_y
Cuts at: 1493 2288 2833 2883 2999 3115 3341 3493 5602
Size: 795 545 50 116 116 226 152 2109
Cuts at: 5602 1493
Size: 1697
Fragments arranged by size:
2109 1697 795 545 226 152 116 116
50
AseI AT'TA_AT
Cuts at: 160 1032 2571 4360 160
Size: 872 1539 1789 1606
Fragments arranged by size:
1789 1606 1539 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5806
AvaI C'yCGr_G
Cuts at: 1870 1943 3131 1870
Size: 73 1188 4545
Fragments arranged by size:
4545 1188 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2317 2350 2317
Size: 33 5773
BamHI G'GATC_C
Cuts at: 3136 3503 3136
Size: 367 5439
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2333 2375 2794 3127 4582
Size: 325 217 627 546 42 419 333 1455
Cuts at: 4582 618
Size: 1842
Fragments arranged by size:
1842 1455 627 546 419 333 325 217
42
BanII G_rGCy'C
Cuts at: 1391 3125 1391
Size: 1734 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4835
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3997 4031 1903
Size: 34 2060 34 3678
Fragments arranged by size:
3678 2060 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3698 4908 3698
Size: 1210 4596
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5806
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4417 136
Size: 107 122 71 631 605 2745 1525
Fragments arranged by size:
2745 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2856 5801 2856
Size: 2945 2861
BlpI GC'TnA_GC
Cuts at: 2112 3223 2112
Size: 1111 4695
Bme1580I G_kGCm'C
Cuts at: 1790 3867 5023 1790
Size: 2077 1156 2573
Fragments arranged by size:
2573 2077 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5806
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2921 3537 4495
Size: 416 53 83 399 24 1504 616 958
Cuts at: 4495 442
Size: 1753
Fragments arranged by size:
1753 1504 958 616 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5806
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2929 4466 1239
Size: 1690 1537 2579
Fragments arranged by size:
2579 1690 1537
Bpu10I CC'TnA_GC
Cuts at: 2017 2017
Size: 5806
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3931 4796 5094 3931
Size: 865 298 4643
Fragments arranged by size:
4643 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3029 4469 882
Size: 617 1530 1440 2219
Fragments arranged by size:
2219 1530 1440 617
BsaAI yAC'GTr
Cuts at: 493 1204 2669 3597 493
Size: 711 1465 928 2702
Fragments arranged by size:
2702 1465 928 711
BsaBI GATnn'nnATC
Cuts at: 5800 5800
Size: 5806
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3613 3995 4630 275
Size: 53 83 186 936 2080 382 635 1451
Fragments arranged by size:
2080 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4239 4637 4911 5058 5469 4239
Size: 398 274 147 411 4576
Fragments arranged by size:
4576 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2747 2777 1178
Size: 30 165 30 1344 30 4207
Fragments arranged by size:
4207 1344 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5806
BseYI C'CCAG_C
Cuts at: 5009 5505 5009
Size: 496 5310
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5233
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3256 4017 5045 1540
Size: 275 186 1255 761 1028 2301
Fragments arranged by size:
2301 1255 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3867 3952 5023 3867
Size: 85 1071 4650
Fragments arranged by size:
4650 1071 85
BsmI GAATG_Cn'
Cuts at: 2511 2625 3317 3410 2511
Size: 114 692 93 4907
Fragments arranged by size:
4907 692 114 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3125 3867 3952 5023 1391
Size: 399 1335 742 85 1071 2174
Fragments arranged by size:
2174 1335 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5469 5469
Size: 5806
BspHI T'CATG_A
Cuts at: 3695 5425 3695
Size: 1730 4076
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3161 844
Size: 2317 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3693 1394
Size: 429 1870 3507
Fragments arranged by size:
3507 1870 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2409 4301 4475 65
Size: 1183 1161 1892 174 1396
Fragments arranged by size:
1892 1396 1183 1161 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4450 4637 1700
Size: 110 2640 187 2869
Fragments arranged by size:
2869 2640 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5806
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5806
BssSI C'ACGA_G
Cuts at: 3866 4878 3866
Size: 1012 4794
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5806
BstBI TT'CG_AA
Cuts at: 4610 4610
Size: 5806
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5806
BstYI r'GATC_y
Cuts at: 1972 2340 2353 2856 3136 3503 3888 3905 5357
Size: 368 13 503 280 367 385 17 1452
Cuts at: 5357 5443 5455 5801 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 503 385 368 367 346 280
86 17 13 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5254
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5067
Fragments arranged by size:
5067 683 56
BtsI GCAGTG_nn'
Cuts at: 2240 2421 3322 4116 4136 2240
Size: 181 901 794 20 3910
Fragments arranged by size:
3910 901 794 181 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5806
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5771
DraI TTT'AAA
Cuts at: 3004 3110 3004
Size: 106 5700
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4813 809
Size: 507 3497 1802
Fragments arranged by size:
3497 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2181 3253 4142
Size: 54 1425 50 275 18 351 1072 889
Cuts at: 4142 5771 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1072 889 351 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3253 1537
Size: 275 1441 4090
Fragments arranged by size:
4090 1441 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2615 3736 1353
Size: 1262 1121 3423
Fragments arranged by size:
3423 1262 1121
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4393 4767 4913 387
Size: 1064 138 2804 374 146 1280
Fragments arranged by size:
2804 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2929 3869 4466 5253 5521 1239
Size: 393 1297 940 597 787 268 1524
Fragments arranged by size:
1524 1297 940 787 597 393 268
EcoRI G'AATT_C
Cuts at: 2288 3115 3493 2288
Size: 827 378 4601
Fragments arranged by size:
4601 827 378
EcoRV GAT'ATC
Cuts at: 2782 2782
Size: 5806
FspI TGC'GCA
Cuts at: 4312 4312
Size: 5806
HaeII r_GCGC'y
Cuts at: 1678 4953 1678
Size: 3275 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2709 2741 2747 2779 2906
Size: 32 135 32 1137 32 6 32 127
Cuts at: 2906 2938 4447 4479 4521 4553 1373
Size: 32 1509 32 42 32 2626
Fragments arranged by size:
2626 1509 1137 135 127 42 32 32
32 32 32 32 32 6
HincII GTy'rAC
Cuts at: 669 1657 1938 2238 3150 3396 669
Size: 988 281 300 912 246 3079
Fragments arranged by size:
3079 988 912 300 281 246
HindIII A'AGCT_T
Cuts at: 748 2114 748
Size: 1366 4440
HpaI GTT'AAC
Cuts at: 3396 3396
Size: 5806
KpnI G_GTAC'C
Cuts at: 3131 3131
Size: 5806
MfeI C'AATT_G
Cuts at: 3405 3405
Size: 5806
MluI A'CGCG_T
Cuts at: 5682 5682
Size: 5806
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3051 4920 5104 1305
Size: 1746 1869 184 2007
Fragments arranged by size:
2007 1869 1746 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2183 10
Size: 54 1425 343 351 3633
Fragments arranged by size:
3633 1425 351 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2414 2699 3764 4123 4282 518
Size: 648 1248 285 1065 359 159 2042
Fragments arranged by size:
2042 1248 1065 648 359 285 159
MspA1I CmG'CkG
Cuts at: 1606 2684 2829 3222 3899 5047 5292 1606
Size: 1078 145 393 677 1148 245 2120
Fragments arranged by size:
2120 1148 1078 677 393 245 145
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5254
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5806
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5806
NotI GC'GGCC_GC
Cuts at: 3253 3253
Size: 5806
NspI r_CATG'y
Cuts at: 1132 1229 3164 4709 1132
Size: 97 1935 1545 2229
Fragments arranged by size:
2229 1935 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5806
PciI A'CATG_T
Cuts at: 4705 4705
Size: 5806
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2144 2350 3070 1108
Size: 1036 206 720 3844
Fragments arranged by size:
3844 1036 720 206
PfoI T'CCnGG_A
Cuts at: 2161 2349 2945 2161
Size: 188 596 5022
Fragments arranged by size:
5022 596 188
PmeI GTTT'AAAC
Cuts at: 3110 3110
Size: 5806
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3875 3907 5388 5420 3875
Size: 32 1481 32 4261
Fragments arranged by size:
4261 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5806
PsiI TTA'TAA
Cuts at: 3376 3376
Size: 5806
PstI C_TGCA'G
Cuts at: 830 3158 830
Size: 2328 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5806
PvuII CAG'CTG
Cuts at: 2684 2684
Size: 5806
SalI G'TCGA_C
Cuts at: 1936 3148 1936
Size: 1212 4594
SapI GCTCTTCn'nnn_
Cuts at: 2615 2615
Size: 5806
SbfI CC_TGCA'GG
Cuts at: 3158 3158
Size: 5806
ScaI AGT'ACT
Cuts at: 4054 5766 4054
Size: 1712 4094
SfcI C'TryA_G
Cuts at: 826 1045 2679 3154 4289 4970 5161 826
Size: 219 1634 475 1135 681 191 1471
Fragments arranged by size:
1634 1471 1135 681 475 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5806
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5806
SmaI CCC'GGG
Cuts at: 1872 3133 1872
Size: 1261 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2096 3910 4811 5073 820
Size: 197 926 153 1814 901 262 1553
Fragments arranged by size:
1814 1553 926 901 262 197 153
SnaBI TAC'GTA
Cuts at: 493 2669 3597 493
Size: 2176 928 2702
Fragments arranged by size:
2702 2176 928
SpeI A'CTAG_T
Cuts at: 152 2875 152
Size: 2723 3083
SphI G_CATG'C
Cuts at: 1132 3164 1132
Size: 2032 3774
SspI AAT'ATT
Cuts at: 5 52 3730 5
Size: 47 3678 2081
Fragments arranged by size:
3678 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5806
StyI C'CwwG_G
Cuts at: 513 1065 2379 3245 513
Size: 552 1314 866 3074
Fragments arranged by size:
3074 1314 866 552
SwaI ATTT'AAAT
Cuts at: 3004 3004
Size: 5806
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3833 3850 4003 4188 4633 325
Size: 1255 2253 17 153 185 445 1498
Fragments arranged by size:
2253 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4052 5764 96
Size: 274 80 33 51 1443 2075 1712 138
Fragments arranged by size:
2075 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5766 5798 5766
Size: 32 5774
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5806
XbaI T'CTAG_A
Cuts at: 3142 3142
Size: 5806
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2773 2953 2773
Size: 180 5626
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5806
XmaI C'CCGG_G
Cuts at: 1870 3131 1870
Size: 1261 4545
XmnI GAAnn'nnTTC
Cuts at: 3935 3935
Size: 5806
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3614 276
Size: 53 83 186 936 2080 2468
Fragments arranged by size:
2468 2080 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII
SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI SwaI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoO109I FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI
PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI
SfoI SrfI