Restriction map for
pFN21AB3391
(Circular) (Six-base) MAPSORT of: pFN21AB3391.seq Check: 3131 from: 1 to: 5569
pFN21AB3391 5569 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3379 278
Size: 53 83 186 936 1843 2468
Fragments arranged by size:
2468 1843 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2912 1937
Size: 975 4594
Acc65I G'GTAC_C
Cuts at: 2312 2890 2312
Size: 578 4991
AclI AA'CG_TT
Cuts at: 1563 3696 4069 1563
Size: 2133 373 3063
Fragments arranged by size:
3063 2133 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2379 2386 2519 3632 5016 5284 1632
Size: 747 7 133 1113 1384 268 1917
Fragments arranged by size:
1917 1384 1113 747 268 133 7
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5372
AflIII A'CryG_T
Cuts at: 1203 4468 5445 1203
Size: 3265 977 1327
Fragments arranged by size:
3265 1327 977
AgeI A'CCGG_T
Cuts at: 4400 4400
Size: 5569
AhdI GACnn_n'nnGTC
Cuts at: 2540 4298 2540
Size: 1758 3811
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5569
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5569
AlwNI CAG_nnn'CTG
Cuts at: 3312 4884 3312
Size: 1572 3997
ApaLI G'TGCA_C
Cuts at: 3626 4782 3626
Size: 1156 4413
ApoI r'AATT_y
Cuts at: 1493 2579 2772 2844 2878 3104 3256 5365 1493
Size: 1086 193 72 34 226 152 2109 1697
Fragments arranged by size:
2109 1697 1086 226 193 152 72 34
AseI AT'TA_AT
Cuts at: 160 1032 4123 160
Size: 872 3091 1606
Fragments arranged by size:
3091 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5569
AvaI C'yCGr_G
Cuts at: 1870 1943 2316 2441 2894 1870
Size: 73 373 125 453 4545
Fragments arranged by size:
4545 453 373 125 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2296 2304 2329 2337 2296
Size: 8 25 8 5528
Fragments arranged by size:
5528 25 8 8
BamHI G'GATC_C
Cuts at: 2899 3266 2899
Size: 367 5202
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2312 2890 4345 618
Size: 325 217 627 525 578 1455 1842
Fragments arranged by size:
1842 1455 627 578 525 325 217
BanII G_rGCy'C
Cuts at: 1391 2888 1391
Size: 1497 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4598
BbvCI CC'TCA_GC
Cuts at: 2799 2799
Size: 5569
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3760 3794 1903
Size: 34 1823 34 3678
Fragments arranged by size:
3678 1823 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3461 4671 3461
Size: 1210 4359
BclI T'GATC_A
Cuts at: 1548 2454 2749 1548
Size: 906 295 4368
Fragments arranged by size:
4368 906 295
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2478 4180 136
Size: 107 122 71 631 605 806 1702 1525
Fragments arranged by size:
1702 1525 806 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2697 5564 2697
Size: 2867 2702
BlpI GC'TnA_GC
Cuts at: 2986 2986
Size: 5569
Bme1580I G_kGCm'C
Cuts at: 1790 3630 4786 1790
Size: 1840 1156 2573
Fragments arranged by size:
2573 1840 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5569
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3300 4258 442
Size: 416 53 83 399 24 1883 958 1753
Fragments arranged by size:
1883 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5569
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2789 4229 1239
Size: 1550 1440 2579
Fragments arranged by size:
2579 1550 1440
Bpu10I CC'TnA_GC
Cuts at: 2799 2799
Size: 5569
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3694 4559 4857 3694
Size: 865 298 4406
Fragments arranged by size:
4406 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4232 882
Size: 617 2733 2219
Fragments arranged by size:
2733 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2413 3360 493
Size: 711 1209 947 2702
Fragments arranged by size:
2702 1209 947 711
BsaBI GATnn'nnATC
Cuts at: 5563 5563
Size: 5569
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2470 3376 3758 4393
Size: 53 83 186 936 937 906 382 635
Cuts at: 4393 275
Size: 1451
Fragments arranged by size:
1451 937 936 906 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4002 4400 4674 4821 5232 4002
Size: 398 274 147 411 4339
Fragments arranged by size:
4339 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5344
Fragments arranged by size:
5344 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5569
BseYI C'CCAG_C
Cuts at: 4772 5268 4772
Size: 496 5073
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4996
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3019 3780 4808 1540
Size: 275 186 1018 761 1028 2301
Fragments arranged by size:
2301 1028 1018 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3630 3715 4786 3630
Size: 85 1071 4413
Fragments arranged by size:
4413 1071 85
BsmI GAATG_Cn'
Cuts at: 3080 3173 3080
Size: 93 5476
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2888 3630 3715 4786 1391
Size: 399 1098 742 85 1071 2174
Fragments arranged by size:
2174 1098 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5232 5232
Size: 5569
BspHI T'CATG_A
Cuts at: 3458 5188 3458
Size: 1730 3839
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2160 2823 2924 844
Size: 1316 663 101 3489
Fragments arranged by size:
3489 1316 663 101
BsrBI CCG'CTC
Cuts at: 1394 1823 3456 1394
Size: 429 1633 3507
Fragments arranged by size:
3507 1633 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2131 2586 2623 4064 4238 65
Size: 1183 883 455 37 1441 174 1396
Fragments arranged by size:
1441 1396 1183 883 455 174 37
BsrFI r'CCGG_y
Cuts at: 1700 1810 4213 4400 1700
Size: 110 2403 187 2869
Fragments arranged by size:
2869 2403 187 110
BsrGI T'GTAC_A
Cuts at: 96 2208 96
Size: 2112 3457
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5569
BssSI C'ACGA_G
Cuts at: 3629 4641 3629
Size: 1012 4557
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5569
BstBI TT'CG_AA
Cuts at: 4373 4373
Size: 5569
BstEII G'GTnAC_C
Cuts at: 1184 2093 1184
Size: 909 4660
BstYI r'GATC_y
Cuts at: 1972 2697 2899 3266 3651 3668 5120 5206 5218
Size: 725 202 367 385 17 1452 86 12
Cuts at: 5218 5564 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 725 385 367 346 202 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2386 513
Size: 552 939 382 3696
Fragments arranged by size:
3696 939 552 382
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4830
Fragments arranged by size:
4830 683 56
BtsI GCAGTG_nn'
Cuts at: 3085 3879 3899 3085
Size: 794 20 4755
Fragments arranged by size:
4755 794 20
ClaI AT'CG_AT
Cuts at: 1554 2607 1554
Size: 1053 4516
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5534
DraI TTT'AAA
Cuts at: 2873 2873
Size: 5569
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4576 809
Size: 507 3260 1802
Fragments arranged by size:
3260 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3016 3905 5534
Size: 54 1425 50 275 18 1186 889 1629
Cuts at: 5534 8
Size: 43
Fragments arranged by size:
1629 1425 1186 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3016 1537
Size: 275 1204 4090
Fragments arranged by size:
4090 1204 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3499 1353
Size: 2146 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4156 4530 4676 387
Size: 1064 138 2567 374 146 1280
Fragments arranged by size:
2567 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2379 2386 2519 2789 3632 4229 5016
Size: 393 747 7 133 270 843 597 787
Cuts at: 5016 5284 1239
Size: 268 1524
Fragments arranged by size:
1524 843 787 747 597 393 270 268
133 7
EcoRI G'AATT_C
Cuts at: 2878 3256 2878
Size: 378 5191
EcoRV GAT'ATC
Cuts at: 2611 2611
Size: 5569
FspI TGC'GCA
Cuts at: 4075 4075
Size: 5569
HaeII r_GCGC'y
Cuts at: 1678 4716 1678
Size: 3038 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2521 2526 2553 2558 4210
Size: 32 135 32 949 5 27 5 1652
Cuts at: 4210 4242 4284 4316 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1652 949 135 42 32 32 32
32 27 5 5
HincII GTy'rAC
Cuts at: 669 1657 1938 2913 3159 669
Size: 988 281 975 246 3079
Fragments arranged by size:
3079 988 975 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5569
HpaI GTT'AAC
Cuts at: 3159 3159
Size: 5569
KpnI G_GTAC'C
Cuts at: 2316 2894 2316
Size: 578 4991
MfeI C'AATT_G
Cuts at: 3168 3168
Size: 5569
MluI A'CGCG_T
Cuts at: 5445 5445
Size: 5569
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4683 4867 1305
Size: 3378 184 2007
Fragments arranged by size:
3378 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3747
Fragments arranged by size:
3747 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3527 3886 4045 518
Size: 648 2361 359 159 2042
Fragments arranged by size:
2361 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2506 2985 3662 4810 5055 1606
Size: 900 479 677 1148 245 2120
Fragments arranged by size:
2120 1148 900 677 479 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4078
Fragments arranged by size:
4078 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5569
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5569
NotI GC'GGCC_GC
Cuts at: 3016 3016
Size: 5569
NspI r_CATG'y
Cuts at: 1132 1229 2927 4472 1132
Size: 97 1698 1545 2229
Fragments arranged by size:
2229 1698 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5569
PciI A'CATG_T
Cuts at: 4468 4468
Size: 5569
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2294 1108
Size: 1186 4383
PmeI GTTT'AAAC
Cuts at: 2873 2873
Size: 5569
PmlI CAC'GTG
Cuts at: 2413 2413
Size: 5569
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3638 3670 5151 5183 3638
Size: 32 1481 32 4024
Fragments arranged by size:
4024 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5569
PsiI TTA'TAA
Cuts at: 3139 3139
Size: 5569
PstI C_TGCA'G
Cuts at: 830 2921 830
Size: 2091 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5569
PvuII CAG'CTG
Cuts at: 2506 2506
Size: 5569
SalI G'TCGA_C
Cuts at: 1936 2911 1936
Size: 975 4594
SbfI CC_TGCA'GG
Cuts at: 2921 2921
Size: 5569
ScaI AGT'ACT
Cuts at: 2222 3817 5529 2222
Size: 1595 1712 2262
Fragments arranged by size:
2262 1712 1595
SfcI C'TryA_G
Cuts at: 826 1045 2917 4052 4733 4924 826
Size: 219 1872 1135 681 191 1471
Fragments arranged by size:
1872 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5569
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5569
SmaI CCC'GGG
Cuts at: 1872 2318 2443 2896 1872
Size: 446 125 453 4545
Fragments arranged by size:
4545 453 446 125
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3673 4574 4836 820
Size: 197 926 1730 901 262 1553
Fragments arranged by size:
1730 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3360 493
Size: 2867 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5569
SphI G_CATG'C
Cuts at: 1132 2927 1132
Size: 1795 3774
SspI AAT'ATT
Cuts at: 5 52 3493 5
Size: 47 3441 2081
Fragments arranged by size:
3441 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5569
StyI C'CwwG_G
Cuts at: 513 1065 2004 2238 3008 513
Size: 552 939 234 770 3074
Fragments arranged by size:
3074 939 770 552 234
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3596 3613 3766 3951 4396 325
Size: 1255 2016 17 153 185 445 1498
Fragments arranged by size:
2016 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2208 2220 3815
Size: 274 80 33 51 1443 231 12 1595
Cuts at: 3815 5527 96
Size: 1712 138
Fragments arranged by size:
1712 1595 1443 274 231 138 80 51
33 12
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5529 5561 5529
Size: 32 5537
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5569
XbaI T'CTAG_A
Cuts at: 2905 2905
Size: 5569
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5569
XmaI C'CCGG_G
Cuts at: 1870 2316 2441 2894 1870
Size: 446 125 453 4545
Fragments arranged by size:
4545 453 446 125
XmnI GAAnn'nnTTC
Cuts at: 3698 3698
Size: 5569
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3377 276
Size: 53 83 186 936 1843 2468
Fragments arranged by size:
2468 1843 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoRI EcoRV FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PmlI PpiI PshAI PsiI PstI PvuI
PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoO109I FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PpuMI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI XcmI