Restriction map for
pFN21AB3372
(Circular) (Six-base) MAPSORT of: pFN21AB3372.seq Check: 7410 from: 1 to: 6040
pFN21AB3372 6040 bp
With 182 enzymes: SgfI *
October 20, 2009 10:34 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3850 278
Size: 53 83 186 936 2314 2468
Fragments arranged by size:
2468 2314 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2195 2682 3383 1937
Size: 258 487 701 4594
Fragments arranged by size:
4594 701 487 258
Acc65I G'GTAC_C
Cuts at: 3361 3361
Size: 6040
AclI AA'CG_TT
Cuts at: 1563 4167 4540 1563
Size: 2604 373 3063
Fragments arranged by size:
3063 2604 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2177 2896 4103 5487 5755 1632
Size: 545 719 1207 1384 268 1917
Fragments arranged by size:
1917 1384 1207 719 545 268
AflII C'TTAA_G
Cuts at: 820 1017 3061 820
Size: 197 2044 3799
Fragments arranged by size:
3799 2044 197
AflIII A'CryG_T
Cuts at: 1203 2451 4939 5916 1203
Size: 1248 2488 977 1327
Fragments arranged by size:
2488 1327 1248 977
AgeI A'CCGG_T
Cuts at: 4871 4871
Size: 6040
AhdI GACnn_n'nnGTC
Cuts at: 2191 4769 2191
Size: 2578 3462
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6040
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2604 1184
Size: 1420 4620
AlwNI CAG_nnn'CTG
Cuts at: 2470 3783 5355 2470
Size: 1313 1572 3155
Fragments arranged by size:
3155 1572 1313
ApaLI G'TGCA_C
Cuts at: 4097 5253 4097
Size: 1156 4884
ApoI r'AATT_y
Cuts at: 1493 2052 3083 3349 3575 3727 5836 1493
Size: 559 1031 266 226 152 2109 1697
Fragments arranged by size:
2109 1697 1031 559 266 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4594 160
Size: 872 3562 1606
Fragments arranged by size:
3562 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6040
AvaI C'yCGr_G
Cuts at: 1870 1943 2521 3365 1870
Size: 73 578 844 4545
Fragments arranged by size:
4545 844 578 73
BamHI G'GATC_C
Cuts at: 3370 3737 3370
Size: 367 5673
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2237 3149 3361 4816 618
Size: 325 217 627 450 912 212 1455 1842
Fragments arranged by size:
1842 1455 912 627 450 325 217 212
BanII G_rGCy'C
Cuts at: 1391 2361 2910 3144 3359 1391
Size: 970 549 234 215 4072
Fragments arranged by size:
4072 970 549 234 215
BbeI G_GCGC'C
Cuts at: 2241 2241
Size: 6040
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5069
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4231 4265 1903
Size: 34 2294 34 3678
Fragments arranged by size:
3678 2294 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2075 2865 3932 5142 2075
Size: 790 1067 1210 2973
Fragments arranged by size:
2973 1210 1067 790
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6040
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2174 2562 4651
Size: 107 122 71 631 605 502 388 2089
Cuts at: 4651 136
Size: 1525
Fragments arranged by size:
2089 1525 631 605 502 388 122 107
71
BglII A'GATC_T
Cuts at: 6035 6035
Size: 6040
BlpI GC'TnA_GC
Cuts at: 3457 3457
Size: 6040
Bme1580I G_kGCm'C
Cuts at: 1790 4101 5257 1790
Size: 2311 1156 2573
Fragments arranged by size:
2573 2311 1156
BmgBI CAC'GTC
Cuts at: 1318 2893 1318
Size: 1575 4465
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3233 3771 4729
Size: 416 53 83 399 24 1816 538 958
Cuts at: 4729 442
Size: 1753
Fragments arranged by size:
1816 1753 958 538 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6040
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2536 2536
Size: 6040
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2300 3017 3174 3249 4700 1239
Size: 1061 717 157 75 1451 2579
Fragments arranged by size:
2579 1451 1061 717 157 75
Bpu10I CC'TnA_GC
Cuts at: 2845 2922 3138 2845
Size: 77 216 5747
Fragments arranged by size:
5747 216 77
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2843 4165 5030 5328 2843
Size: 1322 865 298 3555
Fragments arranged by size:
3555 1322 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4703 882
Size: 617 3204 2219
Fragments arranged by size:
3204 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2454 3831 493
Size: 711 1250 1377 2702
Fragments arranged by size:
2702 1377 1250 711
BsaBI GATnn'nnATC
Cuts at: 2150 6034 2150
Size: 3884 2156
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2238 2990 3847 4229
Size: 53 83 186 936 705 752 857 382
Cuts at: 4229 4864 275
Size: 635 1451
Fragments arranged by size:
1451 936 857 752 705 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2442 4473 4871 5145 5292 5703 2442
Size: 2031 398 274 147 411 2779
Fragments arranged by size:
2779 2031 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2705 2735 1178
Size: 30 165 30 1302 30 4483
Fragments arranged by size:
4483 1302 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2952 1186
Size: 1766 4274
BseYI C'CCAG_C
Cuts at: 2551 5243 5739 2551
Size: 2692 496 2852
Fragments arranged by size:
2852 2692 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2078 2119 2693 2941 1163
Size: 573 342 41 574 248 4262
Fragments arranged by size:
4262 574 573 342 248 41
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2128 3490 4251 5279 1540
Size: 275 186 127 1362 761 1028 2301
Fragments arranged by size:
2301 1362 1028 761 275 186 127
BsiHKAI G_wGCw'C
Cuts at: 2361 3144 3327 4101 4186 5257 2361
Size: 783 183 774 85 1071 3144
Fragments arranged by size:
3144 1071 783 774 183 85
BsmI GAATG_Cn'
Cuts at: 2798 3551 3644 2798
Size: 753 93 5194
Fragments arranged by size:
5194 753 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2540 2725 2540
Size: 185 5855
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2361 2910 3144 3327 3359 4101 4186
Size: 399 571 549 234 183 32 742 85
Cuts at: 4186 5257 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 571 549 399 234 183
85 32
BspEI T'CCGG_A
Cuts at: 5703 5703
Size: 6040
BspHI T'CATG_A
Cuts at: 2079 3929 5659 2079
Size: 1850 1730 2460
Fragments arranged by size:
2460 1850 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3395 844
Size: 2551 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2528 2659 3927 1394
Size: 429 705 131 1268 3507
Fragments arranged by size:
3507 1268 705 429 131
BsrDI GCAATG_nn'
Cuts at: 65 1248 4535 4709 65
Size: 1183 3287 174 1396
Fragments arranged by size:
3287 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2351 4684 4871 1700
Size: 110 541 2333 187 2869
Fragments arranged by size:
2869 2333 541 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6040
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6040
BssSI C'ACGA_G
Cuts at: 2210 4100 5112 2210
Size: 1890 1012 3138
Fragments arranged by size:
3138 1890 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2698 794
Size: 1904 4136
BstBI TT'CG_AA
Cuts at: 4844 4844
Size: 6040
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6040
BstYI r'GATC_y
Cuts at: 1972 2505 3024 3370 3737 4122 4139 5591 5677
Size: 533 519 346 367 385 17 1452 86
Cuts at: 5677 5689 6035 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 533 519 385 367 346 346
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2232 2394 2539 2818 3210 513
Size: 552 939 228 162 145 279 392 3343
Fragments arranged by size:
3343 939 552 392 279 228 162 145
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5301
Fragments arranged by size:
5301 683 56
BtsI GCAGTG_nn'
Cuts at: 2326 2468 2728 2967 3556 4350 4370 2326
Size: 142 260 239 589 794 20 3996
Fragments arranged by size:
3996 794 589 260 239 142 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6040
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6005
DraI TTT'AAA
Cuts at: 3344 3344
Size: 6040
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5047 809
Size: 507 3731 1802
Fragments arranged by size:
3731 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2125 2166 3487
Size: 54 1425 50 275 18 295 41 1321
Cuts at: 3487 4376 6005 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1321 889 295 275 54 50
43 41 18
EagI C'GGCC_G
Cuts at: 1537 1812 2125 3487 1537
Size: 275 313 1362 4090
Fragments arranged by size:
4090 1362 313 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2394 3970 1353
Size: 1041 1576 3423
Fragments arranged by size:
3423 1576 1041
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2057 3017 3156 4627 5001 5147
Size: 1064 138 468 960 139 1471 374 146
Cuts at: 5147 387
Size: 1280
Fragments arranged by size:
1471 1280 1064 960 468 374 146 139
138
EcoICRI GAG'CTC
Cuts at: 2359 3142 2359
Size: 783 5257
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2177 2300 2896 3017 3174 3249 4103
Size: 393 545 123 596 121 157 75 854
Cuts at: 4103 4700 5487 5755 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 854 787 597 596 545 393 268
157 123 121 75
EcoNI CCTnn'n_nnAGG
Cuts at: 2861 2861
Size: 6040
EcoO109I rG'GnC_Cy
Cuts at: 2161 2275 2293 2161
Size: 114 18 5908
Fragments arranged by size:
5908 114 18
EcoRI G'AATT_C
Cuts at: 3083 3349 3727 3083
Size: 266 378 5396
Fragments arranged by size:
5396 378 266
EcoRV GAT'ATC
Cuts at: 3164 3164
Size: 6040
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3103 3103
Size: 6040
FspI TGC'GCA
Cuts at: 4546 4546
Size: 6040
HaeII r_GCGC'y
Cuts at: 1678 2117 2241 3104 3284 5187 1678
Size: 439 124 863 180 1903 2531
Fragments arranged by size:
2531 1903 863 439 180 124
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2177 2209 2604 2636 2705
Size: 32 135 32 605 32 395 32 69
Cuts at: 2705 2737 2975 3007 4681 4713 4755 4787 1373
Size: 32 238 32 1674 32 42 32 2626
Fragments arranged by size:
2626 1674 605 395 238 135 69 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2196 3384 3630 669
Size: 988 281 258 1188 246 3079
Fragments arranged by size:
3079 1188 988 281 258 246
HindIII A'AGCT_T
Cuts at: 748 3058 748
Size: 2310 3730
HpaI GTT'AAC
Cuts at: 3630 3630
Size: 6040
KasI G'GCGC_C
Cuts at: 2237 2237
Size: 6040
KpnI G_GTAC'C
Cuts at: 3365 3365
Size: 6040
MfeI C'AATT_G
Cuts at: 3639 3639
Size: 6040
MluI A'CGCG_T
Cuts at: 5916 5916
Size: 6040
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5154 5338 1305
Size: 3849 184 2007
Fragments arranged by size:
3849 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2168 10
Size: 54 1425 343 336 3882
Fragments arranged by size:
3882 1425 343 336 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3239 3998 4357 4516 518
Size: 648 2073 759 359 159 2042
Fragments arranged by size:
2073 2042 759 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2408 3014 3456 4133 5281 5526 1606
Size: 802 606 442 677 1148 245 2120
Fragments arranged by size:
2120 1148 802 677 606 442 245
NaeI GCC'GGC
Cuts at: 2353 2353
Size: 6040
NarI GG'CG_CC
Cuts at: 2238 2238
Size: 6040
NcoI C'CATG_G
Cuts at: 513 1065 2004 3210 513
Size: 552 939 1206 3343
Fragments arranged by size:
3343 1206 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6040
NgoMIV G'CCGG_C
Cuts at: 2351 2351
Size: 6040
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6040
NotI GC'GGCC_GC
Cuts at: 3487 3487
Size: 6040
NspI r_CATG'y
Cuts at: 1132 1229 3398 4943 1132
Size: 97 2169 1545 2229
Fragments arranged by size:
2229 2169 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2665 1348
Size: 1317 4723
PciI A'CATG_T
Cuts at: 4939 4939
Size: 6040
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6040
PfoI T'CCnGG_A
Cuts at: 3190 3190
Size: 6040
PmeI GTTT'AAAC
Cuts at: 3344 3344
Size: 6040
PmlI CAC'GTG
Cuts at: 2454 2454
Size: 6040
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4109 4141 5622 5654 4109
Size: 32 1481 32 4495
Fragments arranged by size:
4495 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2161 2275 2161
Size: 114 5926
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6040
PsiI TTA'TAA
Cuts at: 3610 3610
Size: 6040
PstI C_TGCA'G
Cuts at: 830 2392 2694 3208 3392 830
Size: 1562 302 514 184 3478
Fragments arranged by size:
3478 1562 514 302 184
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6040
PvuII CAG'CTG
Cuts at: 3014 3014
Size: 6040
SacI G_AGCT'C
Cuts at: 2361 3144 2361
Size: 783 5257
SalI G'TCGA_C
Cuts at: 1936 2194 3382 1936
Size: 258 1188 4594
Fragments arranged by size:
4594 1188 258
SbfI CC_TGCA'GG
Cuts at: 2694 3392 2694
Size: 698 5342
ScaI AGT'ACT
Cuts at: 4288 6000 4288
Size: 1712 4328
SfcI C'TryA_G
Cuts at: 826 1045 2388 2690 3204 3388 4523 5204 5395
Size: 219 1343 302 514 184 1135 681 191
Cuts at: 5395 826
Size: 1471
Fragments arranged by size:
1471 1343 1135 681 514 302 219 191
184
SfiI GGCCn_nnn'nGGCC
Cuts at: 2174 2174
Size: 6040
SfoI GGC'GCC
Cuts at: 2239 2239
Size: 6040
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6040
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6040
SmaI CCC'GGG
Cuts at: 1872 3367 1872
Size: 1495 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2858 3061 4144 5045 5307 820
Size: 197 926 915 203 1083 901 262 1553
Fragments arranged by size:
1553 1083 926 915 901 262 203 197
SnaBI TAC'GTA
Cuts at: 493 3831 493
Size: 3338 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6040
SphI G_CATG'C
Cuts at: 1132 3398 1132
Size: 2266 3774
SspI AAT'ATT
Cuts at: 5 52 3964 5
Size: 47 3912 2081
Fragments arranged by size:
3912 2081 47
StuI AGG'CCT
Cuts at: 1517 2606 1517
Size: 1089 4951
StyI C'CwwG_G
Cuts at: 513 1065 2004 2601 3104 3210 3479 513
Size: 552 939 597 503 106 269 3074
Fragments arranged by size:
3074 939 597 552 503 269 106
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3170 4067 4084 4237 4422 4867 325
Size: 1255 1590 897 17 153 185 445 1498
Fragments arranged by size:
1590 1498 1255 897 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4286 5998 96
Size: 274 80 33 51 1443 2309 1712 138
Fragments arranged by size:
2309 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2305 2337 2355 2387 6000 6032 2305
Size: 32 18 32 3613 32 2313
Fragments arranged by size:
3613 2313 32 32 32 18
Tth111I GACn'n_nGTC
Cuts at: 1316 2717 1316
Size: 1401 4639
XbaI T'CTAG_A
Cuts at: 3376 3376
Size: 6040
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2275 2011
Size: 264 5776
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6040
XmaI C'CCGG_G
Cuts at: 1870 3365 1870
Size: 1495 4545
XmnI GAAnn'nnTTC
Cuts at: 4169 4169
Size: 6040
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3848 276
Size: 53 83 186 936 2314 2468
Fragments arranged by size:
2468 2314 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SacI SalI SbfI ScaI
SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BsiWI BstXI BstZ17I Bsu36I DraIII FseI FspAI
NruI NsiI PacI PspOMI PspXI PsrI RsrII SacII
SanDI SapI SexAI SrfI SwaI