Restriction map for
pFN21AB3356
(Circular) (Six-base) MAPSORT of: pFN21AB3356.seq Check: 6514 from: 1 to: 7282
pFN21AB3356 7282 bp
With 182 enzymes: SgfI *
August 20, 2012 11:36 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5092 278
Size: 53 83 186 936 3556 2468
Fragments arranged by size:
3556 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2539 4625 1937
Size: 602 2086 4594
Fragments arranged by size:
4594 2086 602
Acc65I G'GTAC_C
Cuts at: 4603 4603
Size: 7282
AclI AA'CG_TT
Cuts at: 1563 5409 5782 1563
Size: 3846 373 3063
Fragments arranged by size:
3846 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3756 4338 5345 6729 6997 1632
Size: 2124 582 1007 1384 268 1917
Fragments arranged by size:
2124 1917 1384 1007 582 268
AfeI AGC'GCT
Cuts at: 4487 4487
Size: 7282
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7085
AflIII A'CryG_T
Cuts at: 1203 2777 6181 7158 1203
Size: 1574 3404 977 1327
Fragments arranged by size:
3404 1574 1327 977
AgeI A'CCGG_T
Cuts at: 6113 6113
Size: 7282
AhdI GACnn_n'nnGTC
Cuts at: 6011 6011
Size: 7282
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7282
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7282
AlwNI CAG_nnn'CTG
Cuts at: 5025 6597 5025
Size: 1572 5710
ApaLI G'TGCA_C
Cuts at: 4188 5339 6495 4188
Size: 1151 1156 4975
Fragments arranged by size:
4975 1156 1151
ApoI r'AATT_y
Cuts at: 1493 3254 3367 4591 4817 4969 7078 1493
Size: 1761 113 1224 226 152 2109 1697
Fragments arranged by size:
2109 1761 1697 1224 226 152 113
AseI AT'TA_AT
Cuts at: 160 1032 5836 160
Size: 872 4804 1606
Fragments arranged by size:
4804 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7282
AvaI C'yCGr_G
Cuts at: 1870 1943 4104 4607 1870
Size: 73 2161 503 4545
Fragments arranged by size:
4545 2161 503 73
AvrII C'CTAG_G
Cuts at: 2746 2746
Size: 7282
BamHI G'GATC_C
Cuts at: 2300 4612 4979 2300
Size: 2312 367 4603
Fragments arranged by size:
4603 2312 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4603 6058 618
Size: 325 217 627 2816 1455 1842
Fragments arranged by size:
2816 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 4601 1391
Size: 3210 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 6311
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5473 5507 1903
Size: 34 3536 34 3678
Fragments arranged by size:
3678 3536 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5174 6384 5174
Size: 1210 6072
BclI T'GATC_A
Cuts at: 1548 2509 2881 1548
Size: 961 372 5949
Fragments arranged by size:
5949 961 372
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3327 5893 136
Size: 107 122 71 631 605 1655 2566 1525
Fragments arranged by size:
2566 1655 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2598 7277 2598
Size: 4679 2603
BlpI GC'TnA_GC
Cuts at: 4699 4699
Size: 7282
Bme1580I G_kGCm'C
Cuts at: 1790 4192 5343 6499 1790
Size: 2402 1151 1156 2573
Fragments arranged by size:
2573 2402 1156 1151
BmgBI CAC'GTC
Cuts at: 1318 3842 4172 1318
Size: 2524 330 4428
Fragments arranged by size:
4428 2524 330
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3169 3455 5013
Size: 416 53 83 399 24 1752 286 1558
Cuts at: 5013 5971 442
Size: 958 1753
Fragments arranged by size:
1753 1752 1558 958 416 399 286 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 3746 1056
Size: 2690 4592
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2038 2139 4363 4470 5942 1239
Size: 799 101 2224 107 1472 2579
Fragments arranged by size:
2579 2224 1472 799 107 101
Bpu10I CC'TnA_GC
Cuts at: 3666 3666
Size: 7282
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3405 5407 6272 6570 3405
Size: 2002 865 298 4117
Fragments arranged by size:
4117 2002 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3871 5945 882
Size: 617 2372 2074 2219
Fragments arranged by size:
2372 2219 2074 617
BsaAI yAC'GTr
Cuts at: 493 1204 5073 493
Size: 711 3869 2702
Fragments arranged by size:
3869 2702 711
BsaBI GATnn'nnATC
Cuts at: 7276 7276
Size: 7282
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5089 5471 6106 275
Size: 53 83 186 936 3556 382 635 1451
Fragments arranged by size:
3556 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5715 6113 6387 6534 6945 5715
Size: 398 274 147 411 6052
Fragments arranged by size:
6052 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3870 3900 1178
Size: 30 165 30 2467 30 4560
Fragments arranged by size:
4560 2467 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2984 3630 1186
Size: 1798 646 4838
Fragments arranged by size:
4838 1798 646
BseYI C'CCAG_C
Cuts at: 2072 4218 6485 6981 2072
Size: 2146 2267 496 2373
Fragments arranged by size:
2373 2267 2146 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6709
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4732 5493 6521 1540
Size: 275 186 2731 761 1028 2301
Fragments arranged by size:
2731 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2024 2095 2173 4192 5343 5428 6499 2024
Size: 71 78 2019 1151 85 1071 2807
Fragments arranged by size:
2807 2019 1151 1071 85 78 71
BsmI GAATG_Cn'
Cuts at: 3088 4793 4886 3088
Size: 1705 93 5484
Fragments arranged by size:
5484 1705 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2024 2095 2173 4192 4601 5343 5428
Size: 399 234 71 78 2019 409 742 85
Cuts at: 5428 6499 1391
Size: 1071 2174
Fragments arranged by size:
2174 2019 1071 742 409 399 234 85
78 71
BspEI T'CCGG_A
Cuts at: 6945 6945
Size: 7282
BspHI T'CATG_A
Cuts at: 5171 6901 5171
Size: 1730 5552
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4637 844
Size: 3793 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 5169 1394
Size: 429 3346 3507
Fragments arranged by size:
3507 3346 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2792 3202 3316 5777 5951 65
Size: 1183 1544 410 114 2461 174 1396
Fragments arranged by size:
2461 1544 1396 1183 410 174 114
BsrFI r'CCGG_y
Cuts at: 1700 1810 5926 6113 1700
Size: 110 4116 187 2869
Fragments arranged by size:
4116 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7282
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7282
BssSI C'ACGA_G
Cuts at: 5342 6354 5342
Size: 1012 6270
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7282
BstBI TT'CG_AA
Cuts at: 6086 6086
Size: 7282
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7282
BstXI CCAn_nnnn'nTGG
Cuts at: 2550 3119 2550
Size: 569 6713
BstYI r'GATC_y
Cuts at: 1972 2300 2598 4349 4612 4979 5364 5381 6833
Size: 328 298 1751 263 367 385 17 1452
Cuts at: 6833 6919 6931 7277 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1751 1452 385 367 346 328 298
263 86 17 12
BstZ17I GTA'TAC
Cuts at: 2540 2540
Size: 7282
BtgI C'CryG_G
Cuts at: 513 1065 2004 2036 3134 3951 513
Size: 552 939 32 1098 817 3844
Fragments arranged by size:
3844 1098 939 817 552 32
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3459 487
Size: 683 56 2233 4310
Fragments arranged by size:
4310 2233 683 56
BtsI GCAGTG_nn'
Cuts at: 3486 4503 4798 5592 5612 3486
Size: 1017 295 794 20 5156
Fragments arranged by size:
5156 1017 794 295 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7282
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7247
DraI TTT'AAA
Cuts at: 2715 2917 4586 2715
Size: 202 1669 5411
Fragments arranged by size:
5411 1669 202
DraIII CAC_nnn'GTG
Cuts at: 2177 2177
Size: 7282
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6289 809
Size: 507 4973 1802
Fragments arranged by size:
4973 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4729 5618 7247
Size: 54 1425 50 275 18 2899 889 1629
Cuts at: 7247 8
Size: 43
Fragments arranged by size:
2899 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4729 1537
Size: 275 2917 4090
Fragments arranged by size:
4090 2917 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2404 2589 3625 3982 4297 4392 5212 1353
Size: 1051 185 1036 357 315 95 820 3423
Fragments arranged by size:
3423 1051 1036 820 357 315 185 95
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5869 6243 6389 387
Size: 1064 138 4280 374 146 1280
Fragments arranged by size:
4280 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2038 2139 3756 4338 4363 4470 5345
Size: 393 406 101 1617 582 25 107 875
Cuts at: 5345 5942 6729 6997 1239
Size: 597 787 268 1524
Fragments arranged by size:
1617 1524 875 787 597 582 406 393
268 107 101 25
EcoNI CCTnn'n_nnAGG
Cuts at: 3789 3789
Size: 7282
EcoRI G'AATT_C
Cuts at: 4591 4969 4591
Size: 378 6904
FspI TGC'GCA
Cuts at: 5788 5788
Size: 7282
HaeII r_GCGC'y
Cuts at: 1678 4489 6429 1678
Size: 2811 1940 2531
Fragments arranged by size:
2811 2531 1940
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2429 2461 4385 4417 5923
Size: 32 135 32 857 32 1924 32 1506
Cuts at: 5923 5955 5997 6029 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1924 1506 857 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2136 2328 3000 4626 4872 669
Size: 988 281 198 192 672 1626 246 3079
Fragments arranged by size:
3079 1626 988 672 281 246 198 192
HindIII A'AGCT_T
Cuts at: 748 2984 3554 748
Size: 2236 570 4476
Fragments arranged by size:
4476 2236 570
HpaI GTT'AAC
Cuts at: 4872 4872
Size: 7282
KpnI G_GTAC'C
Cuts at: 4607 4607
Size: 7282
MfeI C'AATT_G
Cuts at: 2854 3229 3656 4881 2854
Size: 375 427 1225 5255
Fragments arranged by size:
5255 1225 427 375
MluI A'CGCG_T
Cuts at: 7158 7158
Size: 7282
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2648 6396 6580 1305
Size: 1343 3748 184 2007
Fragments arranged by size:
3748 2007 1343 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5460
Fragments arranged by size:
5460 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2433 3066 3541 5240 5599 5758 518
Size: 648 1267 633 475 1699 359 159 2042
Fragments arranged by size:
2042 1699 1267 648 633 475 359 159
MspA1I CmG'CkG
Cuts at: 1606 2038 4698 5375 6523 6768 1606
Size: 432 2660 677 1148 245 2120
Fragments arranged by size:
2660 2120 1148 677 432 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 3134 513
Size: 552 939 1130 4661
Fragments arranged by size:
4661 1130 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7282
NheI G'CTAG_C
Cuts at: 1052 3742 1052
Size: 2690 4592
NotI GC'GGCC_GC
Cuts at: 4729 4729
Size: 7282
NspI r_CATG'y
Cuts at: 1132 1229 2781 4080 4640 6185 1132
Size: 97 1552 1299 560 1545 2229
Fragments arranged by size:
2229 1552 1545 1299 560 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7282
PciI A'CATG_T
Cuts at: 2777 6181 2777
Size: 3404 3878
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2780 1108
Size: 1672 5610
PfoI T'CCnGG_A
Cuts at: 2845 4447 2845
Size: 1602 5680
PmeI GTTT'AAAC
Cuts at: 4586 4586
Size: 7282
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5351 5383 6864 6896 5351
Size: 32 1481 32 5737
Fragments arranged by size:
5737 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7282
PsiI TTA'TAA
Cuts at: 4852 4852
Size: 7282
PstI C_TGCA'G
Cuts at: 830 4634 830
Size: 3804 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7282
SacII CC_GC'GG
Cuts at: 2039 2039
Size: 7282
SalI G'TCGA_C
Cuts at: 1936 4624 1936
Size: 2688 4594
SbfI CC_TGCA'GG
Cuts at: 4634 4634
Size: 7282
ScaI AGT'ACT
Cuts at: 5530 7242 5530
Size: 1712 5570
SexAI A'CCwGG_T
Cuts at: 2861 2861
Size: 7282
SfcI C'TryA_G
Cuts at: 826 1045 2230 4630 5765 6446 6637 826
Size: 219 1185 2400 1135 681 191 1471
Fragments arranged by size:
2400 1471 1185 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7282
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7282
SmaI CCC'GGG
Cuts at: 1872 4609 1872
Size: 2737 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3420 5386 6287 6549 820
Size: 197 926 1477 1966 901 262 1553
Fragments arranged by size:
1966 1553 1477 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5073 493
Size: 4580 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7282
SphI G_CATG'C
Cuts at: 1132 4640 1132
Size: 3508 3774
SspI AAT'ATT
Cuts at: 5 52 2350 2798 5206 5
Size: 47 2298 448 2408 2081
Fragments arranged by size:
2408 2298 2081 448 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7282
StyI C'CwwG_G
Cuts at: 513 1065 2004 2746 3134 4721 513
Size: 552 939 742 388 1587 3074
Fragments arranged by size:
3074 1587 939 742 552 388
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3397 5309 5326 5479 5664 6109 325
Size: 1255 1817 1912 17 153 185 445 1498
Fragments arranged by size:
1912 1817 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3545 5528 7240
Size: 274 80 33 51 1443 1568 1983 1712
Cuts at: 7240 96
Size: 138
Fragments arranged by size:
1983 1712 1568 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4119 4151 7242 7274 4119
Size: 32 3091 32 4127
Fragments arranged by size:
4127 3091 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7282
XbaI T'CTAG_A
Cuts at: 2447 4618 2447
Size: 2171 5111
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7282
XmaI C'CCGG_G
Cuts at: 1870 4607 1870
Size: 2737 4545
XmnI GAAnn'nnTTC
Cuts at: 3255 3693 5411 3255
Size: 438 1718 5126
Fragments arranged by size:
5126 1718 438
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5090 276
Size: 53 83 186 936 3556 2468
Fragments arranged by size:
3556 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PstI PvuI SacII SalI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI BaeI BbeI BbvCI BfrBI
BplI BsiWI BsmBI Bsu36I EcoICRI EcoO109I EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII
SacI SanDI SapI SfiI SfoI SrfI SwaI XcmI