Restriction map for
pFN21AB3350
(Circular) (Six-base) MAPSORT of: pFN21AB3350.seq Check: 1780 from: 1 to: 6610
pFN21AB3350 6610 bp
With 182 enzymes: SgfI *
February 27, 2009 14:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2049 2049
Size: 6610
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4420 278
Size: 53 83 186 936 2884 2468
Fragments arranged by size:
2884 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3953 1937
Size: 2016 4594
Acc65I G'GTAC_C
Cuts at: 3931 3931
Size: 6610
AclI AA'CG_TT
Cuts at: 1563 3909 4737 5110 1563
Size: 2346 828 373 3063
Fragments arranged by size:
3063 2346 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4673 6057 6325 1632
Size: 3041 1384 268 1917
Fragments arranged by size:
3041 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6413
AflIII A'CryG_T
Cuts at: 1203 3397 5509 6486 1203
Size: 2194 2112 977 1327
Fragments arranged by size:
2194 2112 1327 977
AgeI A'CCGG_T
Cuts at: 5441 5441
Size: 6610
AhdI GACnn_n'nnGTC
Cuts at: 5339 5339
Size: 6610
AleI CACnn'nnGTG
Cuts at: 1166 3041 1166
Size: 1875 4735
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3491 1184
Size: 2307 4303
AlwNI CAG_nnn'CTG
Cuts at: 2431 3283 4353 5925 2431
Size: 852 1070 1572 3116
Fragments arranged by size:
3116 1572 1070 852
ApaI G_GGCC'C
Cuts at: 2361 2361
Size: 6610
ApaLI G'TGCA_C
Cuts at: 3476 4667 5823 3476
Size: 1191 1156 4263
Fragments arranged by size:
4263 1191 1156
ApoI r'AATT_y
Cuts at: 1493 3919 4145 4297 6406 1493
Size: 2426 226 152 2109 1697
Fragments arranged by size:
2426 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5164 160
Size: 872 4132 1606
Fragments arranged by size:
4132 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6610
AvaI C'yCGr_G
Cuts at: 1870 1943 2067 2526 2690 3068 3935 1870
Size: 73 124 459 164 378 867 4545
Fragments arranged by size:
4545 867 459 378 164 124 73
BamHI G'GATC_C
Cuts at: 3940 4307 3940
Size: 367 6243
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2219 3780 3931 5386 618
Size: 325 217 627 432 1561 151 1455 1842
Fragments arranged by size:
1842 1561 1455 627 432 325 217 151
BanII G_rGCy'C
Cuts at: 1391 2361 3056 3140 3929 1391
Size: 970 695 84 789 4072
Fragments arranged by size:
4072 970 789 695 84
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3129 928
Size: 971 1230 4409
Fragments arranged by size:
4409 1230 971
BbvCI CC'TCA_GC
Cuts at: 2460 2460
Size: 6610
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4801 4835 1903
Size: 34 2864 34 3678
Fragments arranged by size:
3678 2864 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4502 5712 4502
Size: 1210 5400
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6610
BfrBI ATG'CAT
Cuts at: 3633 3633
Size: 6610
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5221 136
Size: 107 122 71 631 605 3549 1525
Fragments arranged by size:
3549 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3484 6605 3484
Size: 3121 3489
BlpI GC'TnA_GC
Cuts at: 4027 4027
Size: 6610
Bme1580I G_kGCm'C
Cuts at: 1790 2361 3480 3785 4671 5827 1790
Size: 571 1119 305 886 1156 2573
Fragments arranged by size:
2573 1156 1119 886 571 305
BmgBI CAC'GTC
Cuts at: 1318 3901 1318
Size: 2583 4027
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2364 3143 4341
Size: 416 53 83 399 24 947 779 1198
Cuts at: 4341 5299 442
Size: 958 1753
Fragments arranged by size:
1753 1198 958 947 779 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6610
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2453 2486 3637 3691 5270 1239
Size: 1214 33 1151 54 1579 2579
Fragments arranged by size:
2579 1579 1214 1151 54 33
Bpu10I CC'TnA_GC
Cuts at: 2460 2460
Size: 6610
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2297 2860 4735 5600 5898 2297
Size: 563 1875 865 298 3009
Fragments arranged by size:
3009 1875 865 563 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5273 882
Size: 617 3774 2219
Fragments arranged by size:
3774 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4401 493
Size: 711 3197 2702
Fragments arranged by size:
3197 2702 711
BsaBI GATnn'nnATC
Cuts at: 6604 6604
Size: 6610
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4417 4799 5434 275
Size: 53 83 186 936 2884 382 635 1451
Fragments arranged by size:
2884 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3625 5043 5441 5715 5862 6273 3625
Size: 1418 398 274 147 411 3962
Fragments arranged by size:
3962 1418 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2264 2294 2591 2621 3635
Size: 30 165 30 861 30 297 30 1014
Cuts at: 3635 3665 1178
Size: 30 4123
Fragments arranged by size:
4123 1014 861 297 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2087 2300 3086 3476 1186
Size: 901 213 786 390 4320
Fragments arranged by size:
4320 901 786 390 213
BseYI C'CCAG_C
Cuts at: 5813 6309 5813
Size: 496 6114
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2363 2612 2630 1163
Size: 573 627 249 18 5143
Fragments arranged by size:
5143 627 573 249 18
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4060 4821 5849 1540
Size: 275 186 2059 761 1028 2301
Fragments arranged by size:
2301 2059 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3480 4671 4756 5827 3480
Size: 1191 85 1071 4263
Fragments arranged by size:
4263 1191 1071 85
BsmI GAATG_Cn'
Cuts at: 3635 3698 4121 4214 3635
Size: 63 423 93 6031
Fragments arranged by size:
6031 423 93 63
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2361 3056 3140 3480 3785 3929 4671
Size: 399 571 695 84 340 305 144 742
Cuts at: 4671 4756 5827 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 695 571 399 340 305
144 85 84
BspEI T'CCGG_A
Cuts at: 3625 6273 3625
Size: 2648 3962
BspHI T'CATG_A
Cuts at: 3816 4499 6229 3816
Size: 683 1730 4197
Fragments arranged by size:
4197 1730 683
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2049 2424 3965 844
Size: 1205 375 1541 3489
Fragments arranged by size:
3489 1541 1205 375
BsrBI CCG'CTC
Cuts at: 1394 1823 2206 3239 3772 4497 1394
Size: 429 383 1033 533 725 3507
Fragments arranged by size:
3507 1033 725 533 429 383
BsrDI GCAATG_nn'
Cuts at: 65 1248 5105 5279 65
Size: 1183 3857 174 1396
Fragments arranged by size:
3857 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2234 2328 2940 5254 5441 1700
Size: 110 424 94 612 2314 187 2869
Fragments arranged by size:
2869 2314 612 424 187 110 94
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6610
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6610
BssSI C'ACGA_G
Cuts at: 4670 5682 4670
Size: 1012 5598
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3563 794
Size: 2769 3841
BstBI TT'CG_AA
Cuts at: 5414 5414
Size: 6610
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6610
BstXI CCAn_nnnn'nTGG
Cuts at: 3596 3596
Size: 6610
BstYI r'GATC_y
Cuts at: 1972 3484 3940 4307 4692 4709 6161 6247 6259
Size: 1512 456 367 385 17 1452 86 12
Cuts at: 6259 6605 1972
Size: 346 1977
Fragments arranged by size:
1977 1512 1452 456 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2166 2173 2512 3030 3198 3441
Size: 552 939 162 7 339 518 168 243
Cuts at: 3441 3595 513
Size: 154 3528
Fragments arranged by size:
3528 939 552 518 339 243 168 162
154 7
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2215 3678 487
Size: 683 56 989 1463 3419
Fragments arranged by size:
3419 1463 989 683 56
BtsI GCAGTG_nn'
Cuts at: 3557 3873 4126 4920 4940 3557
Size: 316 253 794 20 5227
Fragments arranged by size:
5227 794 316 253 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6610
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6575
DraI TTT'AAA
Cuts at: 3914 3914
Size: 6610
DraIII CAC_nnn'GTG
Cuts at: 3563 3563
Size: 6610
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5617 809
Size: 507 4301 1802
Fragments arranged by size:
4301 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2908 4057
Size: 54 1425 50 275 18 177 901 1149
Cuts at: 4057 4946 6575 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1149 901 889 275 177 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4057 1537
Size: 275 2245 4090
Fragments arranged by size:
4090 2245 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4540 1353
Size: 3187 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5197 5571 5717 387
Size: 1064 138 3608 374 146 1280
Fragments arranged by size:
3608 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2453 2486 3637 3691 4673 5270 6057
Size: 393 821 33 1151 54 982 597 787
Cuts at: 6057 6325 1239
Size: 268 1524
Fragments arranged by size:
1524 1151 982 821 787 597 393 268
54 33
EcoNI CCTnn'n_nnAGG
Cuts at: 2093 2931 2093
Size: 838 5772
EcoO109I rG'GnC_Cy
Cuts at: 2244 2358 3008 2244
Size: 114 650 5846
Fragments arranged by size:
5846 650 114
EcoRI G'AATT_C
Cuts at: 3919 4297 3919
Size: 378 6232
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2338 2338
Size: 6610
FspI TGC'GCA
Cuts at: 3884 5116 3884
Size: 1232 5378
HaeII r_GCGC'y
Cuts at: 1678 2034 5757 1678
Size: 356 3723 2531
Fragments arranged by size:
3723 2531 356
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3314 3346 3796 3828 5251
Size: 32 135 32 1742 32 450 32 1423
Cuts at: 5251 5283 5325 5357 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1742 1423 450 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3954 4200 669
Size: 988 281 2016 246 3079
Fragments arranged by size:
3079 2016 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6610
HpaI GTT'AAC
Cuts at: 4200 4200
Size: 6610
KpnI G_GTAC'C
Cuts at: 3935 3935
Size: 6610
MfeI C'AATT_G
Cuts at: 4209 4209
Size: 6610
MluI A'CGCG_T
Cuts at: 6486 6486
Size: 6610
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5724 5908 1305
Size: 4419 184 2007
Fragments arranged by size:
4419 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 2910 10
Size: 54 1425 343 177 901 3710
Fragments arranged by size:
3710 1425 901 343 177 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3041 3446 3752 4568 4927 5086 518
Size: 648 1875 405 306 816 359 159 2042
Fragments arranged by size:
2042 1875 816 648 405 359 306 159
MspA1I CmG'CkG
Cuts at: 1606 2016 2464 2861 3283 4026 4703 5851 6096
Size: 410 448 397 422 743 677 1148 245
Cuts at: 6096 1606
Size: 2120
Fragments arranged by size:
2120 1148 743 677 448 422 410 397
245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2512 3030 3441 3595 513
Size: 552 939 508 518 411 154 3528
Fragments arranged by size:
3528 939 552 518 508 411 154
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6610
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6610
NotI GC'GGCC_GC
Cuts at: 4057 4057
Size: 6610
NsiI A_TGCA'T
Cuts at: 3635 3635
Size: 6610
NspI r_CATG'y
Cuts at: 1132 1229 3401 3968 5513 1132
Size: 97 2172 567 1545 2229
Fragments arranged by size:
2229 2172 1545 567 97
PasI CC'CwG_GG
Cuts at: 1348 2991 1348
Size: 1643 4967
PciI A'CATG_T
Cuts at: 3397 5509 3397
Size: 2112 4498
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2906 1108
Size: 1798 4812
PfoI T'CCnGG_A
Cuts at: 3332 3332
Size: 6610
PmeI GTTT'AAAC
Cuts at: 3914 3914
Size: 6610
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4679 4711 6192 6224 4679
Size: 32 1481 32 5065
Fragments arranged by size:
5065 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2244 2244
Size: 6610
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6610
PsiI TTA'TAA
Cuts at: 2742 4180 2742
Size: 1438 5172
PspOMI G'GGCC_C
Cuts at: 2357 2357
Size: 6610
PstI C_TGCA'G
Cuts at: 830 2421 3001 3790 3962 830
Size: 1591 580 789 172 3478
Fragments arranged by size:
3478 1591 789 580 172
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6610
PvuII CAG'CTG
Cuts at: 2464 3283 2464
Size: 819 5791
SalI G'TCGA_C
Cuts at: 1936 3952 1936
Size: 2016 4594
SbfI CC_TGCA'GG
Cuts at: 2421 3790 3962 2421
Size: 1369 172 5069
Fragments arranged by size:
5069 1369 172
ScaI AGT'ACT
Cuts at: 2953 3455 4858 6570 2953
Size: 502 1403 1712 2993
Fragments arranged by size:
2993 1712 1403 502
SexAI A'CCwGG_T
Cuts at: 2088 2088
Size: 6610
SfcI C'TryA_G
Cuts at: 826 1045 2417 2997 3786 3958 5093 5774 5965
Size: 219 1372 580 789 172 1135 681 191
Cuts at: 5965 826
Size: 1471
Fragments arranged by size:
1471 1372 1135 789 681 580 219 191
172
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6610
SgrAI Cr'CCGG_yG
Cuts at: 1700 2234 1700
Size: 534 6076
SmaI CCC'GGG
Cuts at: 1872 2069 3937 1872
Size: 197 1868 4545
Fragments arranged by size:
4545 1868 197
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2312 2875 4714 5615 5877 820
Size: 197 926 369 563 1839 901 262 1553
Fragments arranged by size:
1839 1553 926 901 563 369 262 197
SnaBI TAC'GTA
Cuts at: 493 4401 493
Size: 3908 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6610
SphI G_CATG'C
Cuts at: 1132 3968 1132
Size: 2836 3774
SspI AAT'ATT
Cuts at: 5 52 4534 5
Size: 47 4482 2081
Fragments arranged by size:
4482 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6610
StyI C'CwwG_G
Cuts at: 513 1065 2004 2335 2504 2512 2932 3030 3084
Size: 552 939 331 169 8 420 98 54
Cuts at: 3084 3441 3595 4049 513
Size: 357 154 454 3074
Fragments arranged by size:
3074 939 552 454 420 357 331 169
154 98 54 8
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2138 3117 4637 4654 4807 4992 5437
Size: 1255 558 979 1520 17 153 185 445
Cuts at: 5437 325
Size: 1498
Fragments arranged by size:
1520 1498 1255 979 558 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2951 3453 4856
Size: 274 80 33 51 1443 974 502 1403
Cuts at: 4856 6568 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1403 974 502 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2264 2296 2903 2935 6570 6602 2264
Size: 32 607 32 3635 32 2272
Fragments arranged by size:
3635 2272 607 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2854 1316
Size: 1538 5072
XbaI T'CTAG_A
Cuts at: 3946 3946
Size: 6610
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2017 2291 2017
Size: 274 6336
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6610
XmaI C'CCGG_G
Cuts at: 1870 2067 3935 1870
Size: 197 1868 4545
Fragments arranged by size:
4545 1868 197
XmnI GAAnn'nnTTC
Cuts at: 2321 3426 4739 2321
Size: 1105 1313 4192
Fragments arranged by size:
4192 1313 1105
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4418 276
Size: 53 83 186 936 2884 2468
Fragments arranged by size:
2884 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BplI BsiWI
BsmBI BstZ17I Bsu36I EcoICRI EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NruI PacI PmlI PspXI PsrI
RsrII SacI SacII SanDI SapI SfiI SfoI SrfI
SwaI