Restriction map for
pFN21AB3348
(Circular) (Six-base) MAPSORT of: pFN21AB3348.seq Check: 3088 from: 1 to: 6814
pFN21AB3348 6814 bp
With 182 enzymes: SgfI *
February 27, 2009 14:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2983 2983
Size: 6814
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3423 4624 278
Size: 53 83 186 936 1887 1201 2468
Fragments arranged by size:
2468 1887 1201 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3285 4157 1937
Size: 1348 872 4594
Fragments arranged by size:
4594 1348 872
Acc65I G'GTAC_C
Cuts at: 4135 4135
Size: 6814
AclI AA'CG_TT
Cuts at: 1563 4941 5314 1563
Size: 3378 373 3063
Fragments arranged by size:
3378 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2057 2274 2450 4877 6261 6529 1632
Size: 425 217 176 2427 1384 268 1917
Fragments arranged by size:
2427 1917 1384 425 268 217 176
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6617
AflIII A'CryG_T
Cuts at: 1203 5713 6690 1203
Size: 4510 977 1327
Fragments arranged by size:
4510 1327 977
AgeI A'CCGG_T
Cuts at: 5645 5645
Size: 6814
AhdI GACnn_n'nnGTC
Cuts at: 5543 5543
Size: 6814
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6814
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4068 1184
Size: 2884 3930
AlwNI CAG_nnn'CTG
Cuts at: 3172 3280 3542 3559 3586 3937 4021 4105 4557
Size: 108 262 17 27 351 84 84 452
Cuts at: 4557 6129 3172
Size: 1572 3857
Fragments arranged by size:
3857 1572 452 351 262 108 84 84
27 17
ApaI G_GGCC'C
Cuts at: 2081 3865 2081
Size: 1784 5030
ApaLI G'TGCA_C
Cuts at: 4871 6027 4871
Size: 1156 5658
ApoI r'AATT_y
Cuts at: 1493 3410 3949 4123 4349 4501 6610 1493
Size: 1917 539 174 226 152 2109 1697
Fragments arranged by size:
2109 1917 1697 539 226 174 152
AseI AT'TA_AT
Cuts at: 160 1032 2507 5368 160
Size: 872 1475 2861 1606
Fragments arranged by size:
2861 1606 1475 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6814
AvaI C'yCGr_G
Cuts at: 1870 1943 3858 4139 1870
Size: 73 1915 281 4545
Fragments arranged by size:
4545 1915 281 73
BamHI G'GATC_C
Cuts at: 2676 4144 4511 2676
Size: 1468 367 4979
Fragments arranged by size:
4979 1468 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4135 5590 618
Size: 325 217 627 2348 1455 1842
Fragments arranged by size:
2348 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2081 2248 2337 2366 2377 2768 3216 3865
Size: 690 167 89 29 11 391 448 649
Cuts at: 3865 4133 1391
Size: 268 4072
Fragments arranged by size:
4072 690 649 448 391 268 167 89
29 11
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5843
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2622 2656 3011 3045 5005
Size: 34 50 34 601 34 355 34 1960
Cuts at: 5005 5039 1903
Size: 34 3678
Fragments arranged by size:
3678 1960 601 355 50 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2764 4706 5916 2764
Size: 1942 1210 3662
Fragments arranged by size:
3662 1942 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6814
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2637 5425 136
Size: 107 122 71 631 605 965 2788 1525
Fragments arranged by size:
2788 1525 965 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6809 6809
Size: 6814
BlpI GC'TnA_GC
Cuts at: 2336 2762 2861 3179 3305 4231 2336
Size: 426 99 318 126 926 4919
Fragments arranged by size:
4919 926 426 318 126 99
Bme1580I G_kGCm'C
Cuts at: 1790 2081 3865 3913 4875 6031 1790
Size: 291 1784 48 962 1156 2573
Fragments arranged by size:
2573 1784 1156 962 291 48
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6814
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4545 5503 442
Size: 416 53 83 399 24 3128 958 1753
Fragments arranged by size:
3128 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6814
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2770 2770
Size: 6814
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2144 2391 2422 4013 4046 5474 1239
Size: 905 247 31 1591 33 1428 2579
Fragments arranged by size:
2579 1591 1428 905 247 33 31
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2085 3446 4939 5804 6102 2085
Size: 1361 1493 865 298 2797
Fragments arranged by size:
2797 1493 1361 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2017 3105 3867 5477 882
Size: 617 518 1088 762 1610 2219
Fragments arranged by size:
2219 1610 1088 762 617 518
BsaAI yAC'GTr
Cuts at: 493 1204 2267 4605 493
Size: 711 1063 2338 2702
Fragments arranged by size:
2702 2338 1063 711
BsaBI GATnn'nnATC
Cuts at: 2537 6808 2537
Size: 4271 2543
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3420 4621 5003 5638
Size: 53 83 186 936 1887 1201 382 635
Cuts at: 5638 275
Size: 1451
Fragments arranged by size:
1887 1451 1201 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3674 5247 5645 5919 6066 6477 3674
Size: 1573 398 274 147 411 4011
Fragments arranged by size:
4011 1573 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2187 2217 2868 2898 2915
Size: 30 165 30 784 30 651 30 17
Cuts at: 2915 2945 1178
Size: 30 5047
Fragments arranged by size:
5047 784 651 165 30 30 30 30
30 17
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2447 2649 2686 3062 1186
Size: 1261 202 37 376 4938
Fragments arranged by size:
4938 1261 376 202 37
BseYI C'CCAG_C
Cuts at: 2140 2179 2275 4056 6017 6513 2140
Size: 39 96 1781 1961 496 2441
Fragments arranged by size:
2441 1961 1781 496 96 39
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2882 3137 3185 3293 3614 3941 1163
Size: 573 1146 255 48 108 321 327 4036
Fragments arranged by size:
4036 1146 573 327 321 255 108 48
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4264 5025 6053 1540
Size: 275 186 2263 761 1028 2301
Fragments arranged by size:
2301 2263 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2272 2768 2817 3216 3462 3536 4029 4875 4960
Size: 496 49 399 246 74 493 846 85
Cuts at: 4960 6031 2272
Size: 1071 3055
Fragments arranged by size:
3055 1071 846 496 493 399 246 85
74 49
BsmI GAATG_Cn'
Cuts at: 4325 4418 4325
Size: 93 6721
BsmBI CGTCTCn'nnnn_
Cuts at: 3427 3427
Size: 6814
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2081 2248 2272 2337 2366 2377 2768
Size: 399 291 167 24 65 29 11 391
Cuts at: 2768 2817 3216 3462 3536 3865 3913 4029 4133
Size: 49 399 246 74 329 48 116 104
Cuts at: 4133 4875 4960 6031 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 399 391 329 291
246 167 116 104 85 74 65 49
48 29 24 11
BspEI T'CCGG_A
Cuts at: 6477 6477
Size: 6814
BspHI T'CATG_A
Cuts at: 4703 6433 4703
Size: 1730 5084
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2457 2983 3141 3636 4169 844
Size: 1613 526 158 495 533 3489
Fragments arranged by size:
3489 1613 533 526 495 158
BsrBI CCG'CTC
Cuts at: 1394 1823 2209 4701 1394
Size: 429 386 2492 3507
Fragments arranged by size:
3507 2492 429 386
BsrDI GCAATG_nn'
Cuts at: 65 1248 3316 3693 3852 5309 5483 65
Size: 1183 2068 377 159 1457 174 1396
Fragments arranged by size:
2068 1457 1396 1183 377 174 159
BsrFI r'CCGG_y
Cuts at: 1700 1810 5458 5645 1700
Size: 110 3648 187 2869
Fragments arranged by size:
3648 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2931 96
Size: 2835 3979
BssHII G'CGCG_C
Cuts at: 1925 4006 1925
Size: 2081 4733
BssSI C'ACGA_G
Cuts at: 4874 5886 4874
Size: 1012 5802
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2998 3199 3937 794
Size: 2204 201 738 3671
Fragments arranged by size:
3671 2204 738 201
BstBI TT'CG_AA
Cuts at: 5618 5618
Size: 6814
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6814
BstXI CCAn_nnnn'nTGG
Cuts at: 2118 2118
Size: 6814
BstYI r'GATC_y
Cuts at: 1972 2676 2964 4017 4144 4511 4896 4913 6365
Size: 704 288 1053 127 367 385 17 1452
Cuts at: 6365 6451 6463 6809 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1053 704 385 367 346 288
127 86 17 12
BstZ17I GTA'TAC
Cuts at: 3286 3286
Size: 6814
BtgI C'CryG_G
Cuts at: 513 1065 3878 3955 513
Size: 552 2813 77 3372
Fragments arranged by size:
3372 2813 552 77
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6075
Fragments arranged by size:
6075 683 56
BtsI GCAGTG_nn'
Cuts at: 2195 2982 3619 3929 4330 5124 5144 2195
Size: 787 637 310 401 794 20 3865
Fragments arranged by size:
3865 794 787 637 401 310 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6814
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6779
DraI TTT'AAA
Cuts at: 2802 4118 2802
Size: 1316 5498
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3881 5821 809
Size: 507 2565 1940 1802
Fragments arranged by size:
2565 1940 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2217 2979 3849
Size: 54 1425 50 275 18 387 762 870
Cuts at: 3849 4261 5150 6779 8
Size: 412 889 1629 43
Fragments arranged by size:
1629 1425 889 870 762 412 387 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4261 1537
Size: 275 2449 4090
Fragments arranged by size:
4090 2449 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2603 3524 4744 1353
Size: 1250 921 1220 3423
Fragments arranged by size:
3423 1250 1220 921
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3121 5401 5775 5921 387
Size: 1064 138 1532 2280 374 146 1280
Fragments arranged by size:
2280 1532 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2766 3214 2766
Size: 448 6366
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2057 2144 2274 2391 2422 2450 4013
Size: 393 425 87 130 117 31 28 1563
Cuts at: 4013 4046 4877 5474 6261 6529 1239
Size: 33 831 597 787 268 1524
Fragments arranged by size:
1563 1524 831 787 597 425 393 268
130 117 87 33 31 28
EcoNI CCTnn'n_nnAGG
Cuts at: 3896 3896
Size: 6814
EcoO109I rG'GnC_Cy
Cuts at: 2077 2078 2130 2259 2713 3076 3512 3862 3883
Size: 1 52 129 454 363 436 350 21
Cuts at: 3883 3934 4075 2077
Size: 51 141 4816
Fragments arranged by size:
4816 454 436 363 350 141 129 52
51 21 1
EcoRI G'AATT_C
Cuts at: 3949 4123 4501 3949
Size: 174 378 6262
Fragments arranged by size:
6262 378 174
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3095 3095
Size: 6814
FspI TGC'GCA
Cuts at: 5320 5320
Size: 6814
HaeII r_GCGC'y
Cuts at: 1678 3506 5961 1678
Size: 1828 2455 2531
Fragments arranged by size:
2531 2455 1828
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2235 2267 2868 2900 4062
Size: 32 135 32 663 32 601 32 1162
Cuts at: 4062 4094 5455 5487 5529 5561 1373
Size: 32 1361 32 42 32 2626
Fragments arranged by size:
2626 1361 1162 663 601 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4158 4404 669
Size: 988 281 2220 246 3079
Fragments arranged by size:
3079 2220 988 281 246
HindIII A'AGCT_T
Cuts at: 748 4049 748
Size: 3301 3513
HpaI GTT'AAC
Cuts at: 4404 4404
Size: 6814
KpnI G_GTAC'C
Cuts at: 4139 4139
Size: 6814
MfeI C'AATT_G
Cuts at: 4413 4413
Size: 6814
MluI A'CGCG_T
Cuts at: 6690 6690
Size: 6814
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2992 5928 6112 1305
Size: 1687 2936 184 2007
Fragments arranged by size:
2936 2007 1687 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2219 2981 3851 10
Size: 54 1425 343 387 762 870 2973
Fragments arranged by size:
2973 1425 870 762 387 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2780 3759 4772 5131 5290 518
Size: 648 1614 979 1013 359 159 2042
Fragments arranged by size:
2042 1614 1013 979 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2340 2921 3097 3823 3880 4102 4230 4907
Size: 734 581 176 726 57 222 128 677
Cuts at: 4907 6055 6300 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 734 726 677 581 245 222
176 128 57
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6262
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6814
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6814
NotI GC'GGCC_GC
Cuts at: 4261 4261
Size: 6814
NspI r_CATG'y
Cuts at: 1132 1229 2905 4172 5717 1132
Size: 97 1676 1267 1545 2229
Fragments arranged by size:
2229 1676 1545 1267 97
PasI CC'CwG_GG
Cuts at: 1348 3220 1348
Size: 1872 4942
PciI A'CATG_T
Cuts at: 5713 5713
Size: 6814
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3778 4021 1108
Size: 2670 243 3901
Fragments arranged by size:
3901 2670 243
PfoI T'CCnGG_A
Cuts at: 2368 2679 2727 2967 3771 3891 4029 4062 2368
Size: 311 48 240 804 120 138 33 5120
Fragments arranged by size:
5120 804 311 240 138 120 48 33
PmeI GTTT'AAAC
Cuts at: 4118 4118
Size: 6814
PmlI CAC'GTG
Cuts at: 2267 2267
Size: 6814
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4883 4915 6396 6428 4883
Size: 32 1481 32 5269
Fragments arranged by size:
5269 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2130 2713 3512 3883 4075 2130
Size: 583 799 371 192 4869
Fragments arranged by size:
4869 799 583 371 192
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6814
PsiI TTA'TAA
Cuts at: 4384 4384
Size: 6814
PspOMI G'GGCC_C
Cuts at: 2077 3861 2077
Size: 1784 5030
PstI C_TGCA'G
Cuts at: 830 2626 3024 3195 3325 3399 3555 3711 3942
Size: 1796 398 171 130 74 156 156 231
Cuts at: 3942 3996 4047 4166 830
Size: 54 51 119 3478
Fragments arranged by size:
3478 1796 398 231 171 156 156 130
119 74 54 51
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6814
PvuII CAG'CTG
Cuts at: 2340 3097 3823 2340
Size: 757 726 5331
Fragments arranged by size:
5331 757 726
SacI G_AGCT'C
Cuts at: 2768 3216 2768
Size: 448 6366
SacII CC_GC'GG
Cuts at: 3881 3881
Size: 6814
SalI G'TCGA_C
Cuts at: 1936 4156 1936
Size: 2220 4594
SanDI GG'GwC_CC
Cuts at: 2130 3512 2130
Size: 1382 5432
SapI GCTCTTCn'nnn_
Cuts at: 3524 3524
Size: 6814
SbfI CC_TGCA'GG
Cuts at: 3942 4166 3942
Size: 224 6590
ScaI AGT'ACT
Cuts at: 5062 6774 5062
Size: 1712 5102
SexAI A'CCwGG_T
Cuts at: 2394 2394
Size: 6814
SfcI C'TryA_G
Cuts at: 826 1045 2622 3020 3191 3321 3395 3551 3707
Size: 219 1577 398 171 130 74 156 156
Cuts at: 3707 3938 3992 4043 4162 5297 5978 6169 826
Size: 231 54 51 119 1135 681 191 1471
Fragments arranged by size:
1577 1471 1135 681 398 231 219 191
171 156 156 130 119 74 54 51
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6814
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6814
SmaI CCC'GGG
Cuts at: 1872 3860 4141 1872
Size: 1988 281 4545
Fragments arranged by size:
4545 1988 281
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2100 3461 4918 5819 6081 820
Size: 197 926 157 1361 1457 901 262 1553
Fragments arranged by size:
1553 1457 1361 926 901 262 197 157
SnaBI TAC'GTA
Cuts at: 493 4605 493
Size: 4112 2702
SpeI A'CTAG_T
Cuts at: 152 3905 152
Size: 3753 3061
SphI G_CATG'C
Cuts at: 1132 4172 1132
Size: 3040 3774
SrfI GCCC'GGGC
Cuts at: 3860 3860
Size: 6814
SspI AAT'ATT
Cuts at: 5 52 4738 5
Size: 47 4686 2081
Fragments arranged by size:
4686 2081 47
StuI AGG'CCT
Cuts at: 1517 3454 1517
Size: 1937 4877
StyI C'CwwG_G
Cuts at: 513 1065 2297 4253 513
Size: 552 1232 1956 3074
Fragments arranged by size:
3074 1956 1232 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2486 3233 3363 4841 4858 5011 5196
Size: 1255 906 747 130 1478 17 153 185
Cuts at: 5196 5641 325
Size: 445 1498
Fragments arranged by size:
1498 1478 1255 906 747 445 185 153
130 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2931 5060 6772
Size: 274 80 33 51 1443 954 2129 1712
Cuts at: 6772 96
Size: 138
Fragments arranged by size:
2129 1712 1443 954 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2187 2219 3940 3972 6774 6806 2187
Size: 32 1721 32 2802 32 2195
Fragments arranged by size:
2802 2195 1721 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6814
XbaI T'CTAG_A
Cuts at: 4150 4150
Size: 6814
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6814
XmaI C'CCGG_G
Cuts at: 1870 3858 4139 1870
Size: 1988 281 4545
Fragments arranged by size:
4545 1988 281
XmnI GAAnn'nnTTC
Cuts at: 2955 3332 4943 2955
Size: 377 1611 4826
Fragments arranged by size:
4826 1611 377
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3421 4622 276
Size: 53 83 186 936 1887 1201 2468
Fragments arranged by size:
2468 1887 1201 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
Bpu10I BsiWI Bsu36I DraIII EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PspXI PsrI
RsrII SfiI SfoI SwaI XcmI