Restriction map for
pFN21AB3347
(Circular) (Six-base) MAPSORT of: pFN21AB3347.seq Check: 895 from: 1 to: 6916
pFN21AB3347 6916 bp
With 182 enzymes: SgfI *
February 27, 2009 14:35 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3249 4726 278
Size: 53 83 186 936 1713 1477 2468
Fragments arranged by size:
2468 1713 1477 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4259 1937
Size: 2322 4594
Acc65I G'GTAC_C
Cuts at: 4237 4237
Size: 6916
AclI AA'CG_TT
Cuts at: 1563 2123 5043 5416 1563
Size: 560 2920 373 3063
Fragments arranged by size:
3063 2920 560 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2246 2850 3833 3975 4979 6363 6631 1632
Size: 614 604 983 142 1004 1384 268 1917
Fragments arranged by size:
1917 1384 1004 983 614 604 268 142
AflII C'TTAA_G
Cuts at: 820 1017 2032 820
Size: 197 1015 5704
Fragments arranged by size:
5704 1015 197
AflIII A'CryG_T
Cuts at: 1203 5815 6792 1203
Size: 4612 977 1327
Fragments arranged by size:
4612 1327 977
AgeI A'CCGG_T
Cuts at: 5747 5747
Size: 6916
AhdI GACnn_n'nnGTC
Cuts at: 5645 5645
Size: 6916
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6916
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6916
AlwNI CAG_nnn'CTG
Cuts at: 2096 2674 2731 3070 3229 3394 3978 4659 6231
Size: 578 57 339 159 165 584 681 1572
Cuts at: 6231 2096
Size: 2781
Fragments arranged by size:
2781 1572 681 584 578 339 165 159
57
ApaI G_GGCC'C
Cuts at: 2869 2869
Size: 6916
ApaLI G'TGCA_C
Cuts at: 4973 6129 4973
Size: 1156 5760
ApoI r'AATT_y
Cuts at: 1493 4225 4451 4603 6712 1493
Size: 2732 226 152 2109 1697
Fragments arranged by size:
2732 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5470 160
Size: 872 4438 1606
Fragments arranged by size:
4438 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6916
AvaI C'yCGr_G
Cuts at: 1870 1943 2505 2604 2937 4241 1870
Size: 73 562 99 333 1304 4545
Fragments arranged by size:
4545 1304 562 333 99 73
BamHI G'GATC_C
Cuts at: 4246 4613 4246
Size: 367 6549
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4237 5692 618
Size: 325 217 627 2450 1455 1842
Fragments arranged by size:
2450 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2331 2869 2938 2944 3491 3654 3997 4235
Size: 940 538 69 6 547 163 343 238
Cuts at: 4235 1391
Size: 4072
Fragments arranged by size:
4072 940 547 538 343 238 163 69
6
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2838 3142 3821 928
Size: 971 939 304 679 4023
Fragments arranged by size:
4023 971 939 679 304
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 5107 5141 1903
Size: 34 50 34 3086 34 3678
Fragments arranged by size:
3678 3086 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3556 4808 6018 3556
Size: 1252 1210 4454
Fragments arranged by size:
4454 1252 1210
BclI T'GATC_A
Cuts at: 1548 2946 1548
Size: 1398 5518
BfrBI ATG'CAT
Cuts at: 3849 3849
Size: 6916
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5527 136
Size: 107 122 71 631 605 3855 1525
Fragments arranged by size:
3855 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6911 6911
Size: 6916
BlpI GC'TnA_GC
Cuts at: 4333 4333
Size: 6916
Bme1580I G_kGCm'C
Cuts at: 1790 2869 4977 6133 1790
Size: 1079 2108 1156 2573
Fragments arranged by size:
2573 2108 1156 1079
BmgBI CAC'GTC
Cuts at: 1318 3435 3923 1318
Size: 2117 488 4311
Fragments arranged by size:
4311 2117 488
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2325 2538 4647
Size: 416 53 83 399 24 908 213 2109
Cuts at: 4647 5605 442
Size: 958 1753
Fragments arranged by size:
2109 1753 958 908 416 399 213 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6916
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2753 3146 5576 1239
Size: 1514 393 2430 2579
Fragments arranged by size:
2579 2430 1514 393
Bpu10I CC'TnA_GC
Cuts at: 2109 3602 2109
Size: 1493 5423
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5041 5906 6204 5041
Size: 865 298 5753
Fragments arranged by size:
5753 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2354 3000 3047 5579 882
Size: 617 855 646 47 2532 2219
Fragments arranged by size:
2532 2219 855 646 617 47
BsaAI yAC'GTr
Cuts at: 493 1204 4707 493
Size: 711 3503 2702
Fragments arranged by size:
3503 2702 711
BsaBI GATnn'nnATC
Cuts at: 2466 4110 6910 2466
Size: 1644 2800 2472
Fragments arranged by size:
2800 2472 1644
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3246 4723 5105 5740
Size: 53 83 186 936 1713 1477 382 635
Cuts at: 5740 275
Size: 1451
Fragments arranged by size:
1713 1477 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3031 5349 5747 6021 6168 6579 3031
Size: 2318 398 274 147 411 3368
Fragments arranged by size:
3368 2318 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6691
Fragments arranged by size:
6691 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2065 2720 3334 1186
Size: 879 655 614 4768
Fragments arranged by size:
4768 879 655 614
BseYI C'CCAG_C
Cuts at: 2286 2646 3635 3797 6119 6615 2286
Size: 360 989 162 2322 496 2587
Fragments arranged by size:
2587 2322 989 496 360 162
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2201 3305 3380 1163
Size: 573 465 1104 75 4699
Fragments arranged by size:
4699 1104 573 465 75
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4366 5127 6155 1540
Size: 275 186 2365 761 1028 2301
Fragments arranged by size:
2365 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2487 2938 3502 4977 5062 6133 2487
Size: 451 564 1475 85 1071 3270
Fragments arranged by size:
3270 1475 1071 564 451 85
BsmI GAATG_Cn'
Cuts at: 4427 4520 4427
Size: 93 6823
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2331 2487 2869 2938 2944 3491 3502
Size: 399 541 156 382 69 6 547 11
Cuts at: 3502 3654 3997 4235 4977 5062 6133 1391
Size: 152 343 238 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 547 541 399 382 343
238 156 152 85 69 11 6
BspEI T'CCGG_A
Cuts at: 6579 6579
Size: 6916
BspHI T'CATG_A
Cuts at: 4210 4805 6535 4210
Size: 595 1730 4591
Fragments arranged by size:
4591 1730 595
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4271 844
Size: 3427 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4803 1394
Size: 429 2980 3507
Fragments arranged by size:
3507 2980 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2569 3286 3735 5411 5585 65
Size: 1183 1321 717 449 1676 174 1396
Fragments arranged by size:
1676 1396 1321 1183 717 449 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2037 5560 5747 1700
Size: 110 227 3523 187 2869
Fragments arranged by size:
3523 2869 227 187 110
BsrGI T'GTAC_A
Cuts at: 96 3987 96
Size: 3891 3025
BssHII G'CGCG_C
Cuts at: 1925 2500 1925
Size: 575 6341
BssSI C'ACGA_G
Cuts at: 3568 4976 5988 3568
Size: 1408 1012 4496
Fragments arranged by size:
4496 1408 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2105 794
Size: 1311 5605
BstBI TT'CG_AA
Cuts at: 5720 5720
Size: 6916
BstEII G'GTnAC_C
Cuts at: 1184 2819 3943 1184
Size: 1635 1124 4157
Fragments arranged by size:
4157 1635 1124
BstYI r'GATC_y
Cuts at: 1972 2850 3165 4246 4613 4998 5015 6467 6553
Size: 878 315 1081 367 385 17 1452 86
Cuts at: 6553 6565 6911 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1081 878 385 367 346 315
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3144 3144
Size: 6916
BtgI C'CryG_G
Cuts at: 513 1065 3010 3420 513
Size: 552 1945 410 4009
Fragments arranged by size:
4009 1945 552 410
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6177
Fragments arranged by size:
6177 683 56
BtsI GCAGTG_nn'
Cuts at: 3075 3239 3317 4432 5226 5246 3075
Size: 164 78 1115 794 20 4745
Fragments arranged by size:
4745 1115 794 164 78 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6916
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2299 2334 3596 3631 529
Size: 35 1735 35 1262 35 3814
Fragments arranged by size:
3814 1735 1262 35 35 35
DraI TTT'AAA
Cuts at: 4220 4220
Size: 6916
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3104 3586 5923 809
Size: 507 1788 482 2337 1802
Fragments arranged by size:
2337 1802 1788 507 482
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3120 3291 3379
Size: 54 1425 50 275 18 1290 171 88
Cuts at: 3379 4363 5252 6881 8
Size: 984 889 1629 43
Fragments arranged by size:
1629 1425 1290 984 889 275 171 88
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4363 1537
Size: 275 2551 4090
Fragments arranged by size:
4090 2551 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2050 2222 2404 3951 4846 1353
Size: 697 172 182 1547 895 3423
Fragments arranged by size:
3423 1547 895 697 182 172
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3434 5503 5877 6023 387
Size: 1064 138 1845 2069 374 146 1280
Fragments arranged by size:
2069 1845 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2936 2936
Size: 6916
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2246 2753 2850 3146 3833 3975 4979
Size: 393 614 507 97 296 687 142 1004
Cuts at: 4979 5576 6363 6631 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1004 787 687 614 597 507 393
296 268 142 97
EcoNI CCTnn'n_nnAGG
Cuts at: 2265 2265
Size: 6916
EcoO109I rG'GnC_Cy
Cuts at: 2865 2865
Size: 6916
EcoRI G'AATT_C
Cuts at: 4225 4603 4225
Size: 378 6538
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3838 4223 3838
Size: 385 6531
FspI TGC'GCA
Cuts at: 5422 5422
Size: 6916
HaeII r_GCGC'y
Cuts at: 1678 2713 6063 1678
Size: 1035 3350 2531
Fragments arranged by size:
3350 2531 1035
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2931 2963 3171 3203 3460
Size: 32 135 32 1359 32 208 32 257
Cuts at: 3460 3492 3518 3550 3577 3609 5557 5589 5631
Size: 32 26 32 27 32 1948 32 42
Cuts at: 5631 5663 1373
Size: 32 2626
Fragments arranged by size:
2626 1948 1359 257 208 135 42 32
32 32 32 32 32 32 32 32
27 26
HincII GTy'rAC
Cuts at: 669 1657 1938 2062 4260 4506 669
Size: 988 281 124 2198 246 3079
Fragments arranged by size:
3079 2198 988 281 246 124
HindIII A'AGCT_T
Cuts at: 748 2639 748
Size: 1891 5025
HpaI GTT'AAC
Cuts at: 4506 4506
Size: 6916
KpnI G_GTAC'C
Cuts at: 4241 4241
Size: 6916
MfeI C'AATT_G
Cuts at: 4515 4515
Size: 6916
MluI A'CGCG_T
Cuts at: 6792 6792
Size: 6916
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2860 3852 6030 6214 1305
Size: 1555 992 2178 184 2007
Fragments arranged by size:
2178 2007 1555 992 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3293 10
Size: 54 1425 343 1461 3633
Fragments arranged by size:
3633 1461 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2069 2190 3080 3905 4874 5233 5392
Size: 648 903 121 890 825 969 359 159
Cuts at: 5392 518
Size: 2042
Fragments arranged by size:
2042 969 903 890 825 648 359 159
121
MspA1I CmG'CkG
Cuts at: 1606 2290 2346 2674 2719 2731 2752 2761 3374
Size: 684 56 328 45 12 21 9 613
Cuts at: 3374 4332 5009 6157 6402 1606
Size: 958 677 1148 245 2120
Fragments arranged by size:
2120 1148 958 684 677 613 328 245
56 45 21 12 9
NaeI GCC'GGC
Cuts at: 2039 2039
Size: 6916
NcoI C'CATG_G
Cuts at: 513 1065 3010 513
Size: 552 1945 4419
Fragments arranged by size:
4419 1945 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6916
NgoMIV G'CCGG_C
Cuts at: 2037 2037
Size: 6916
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6916
NotI GC'GGCC_GC
Cuts at: 4363 4363
Size: 6916
NsiI A_TGCA'T
Cuts at: 3851 3851
Size: 6916
NspI r_CATG'y
Cuts at: 1132 1229 2189 2743 2842 4006 4274 5819 1132
Size: 97 960 554 99 1164 268 1545 2229
Fragments arranged by size:
2229 1545 1164 960 554 268 99 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6916
PciI A'CATG_T
Cuts at: 5815 5815
Size: 6916
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2674 1108
Size: 1566 5350
PfoI T'CCnGG_A
Cuts at: 2266 2853 4047 4118 2266
Size: 587 1194 71 5064
Fragments arranged by size:
5064 1194 587 71
PmeI GTTT'AAAC
Cuts at: 4220 4220
Size: 6916
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4985 5017 6498 6530 4985
Size: 32 1481 32 5371
Fragments arranged by size:
5371 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6916
PsiI TTA'TAA
Cuts at: 4486 4486
Size: 6916
PspOMI G'GGCC_C
Cuts at: 2865 2865
Size: 6916
PspXI vC'TCGA_Gb
Cuts at: 2937 2937
Size: 6916
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3992 4024 3992
Size: 32 6884
PstI C_TGCA'G
Cuts at: 830 2766 3234 4268 830
Size: 1936 468 1034 3478
Fragments arranged by size:
3478 1936 1034 468
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6916
PvuII CAG'CTG
Cuts at: 2290 2674 2719 2731 2752 2761 2290
Size: 384 45 12 21 9 6445
Fragments arranged by size:
6445 384 45 21 12 9
SacI G_AGCT'C
Cuts at: 2938 2938
Size: 6916
SalI G'TCGA_C
Cuts at: 1936 4258 1936
Size: 2322 4594
SapI GCTCTTCn'nnn_
Cuts at: 3951 3951
Size: 6916
SbfI CC_TGCA'GG
Cuts at: 4268 4268
Size: 6916
ScaI AGT'ACT
Cuts at: 3952 5164 6876 3952
Size: 1212 1712 3992
Fragments arranged by size:
3992 1712 1212
SfcI C'TryA_G
Cuts at: 826 1045 2762 3230 4264 5399 6080 6271 826
Size: 219 1717 468 1034 1135 681 191 1471
Fragments arranged by size:
1717 1471 1135 1034 681 468 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6916
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6916
SmaI CCC'GGG
Cuts at: 1872 2507 4243 1872
Size: 635 1736 4545
Fragments arranged by size:
4545 1736 635
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2032 2937 5020 5921 6183 820
Size: 197 926 89 905 2083 901 262 1553
Fragments arranged by size:
2083 1553 926 905 901 262 197 89
SnaBI TAC'GTA
Cuts at: 493 4707 493
Size: 4214 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6916
SphI G_CATG'C
Cuts at: 1132 2189 2842 4274 1132
Size: 1057 653 1432 3774
Fragments arranged by size:
3774 1432 1057 653
SspI AAT'ATT
Cuts at: 5 52 4840 5
Size: 47 4788 2081
Fragments arranged by size:
4788 2081 47
StuI AGG'CCT
Cuts at: 1517 2443 1517
Size: 926 5990
StyI C'CwwG_G
Cuts at: 513 1065 2146 3010 4355 513
Size: 552 1081 864 1345 3074
Fragments arranged by size:
3074 1345 1081 864 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2946 3763 4943 4960 5113 5298 5743
Size: 1255 1366 817 1180 17 153 185 445
Cuts at: 5743 325
Size: 1498
Fragments arranged by size:
1498 1366 1255 1180 817 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2209 2357 2371
Size: 274 80 33 51 1443 232 148 14
Cuts at: 2371 3950 3987 5162 6874 96
Size: 1579 37 1175 1712 138
Fragments arranged by size:
1712 1579 1443 1175 274 232 148 138
80 51 37 33 14
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6876 6908 6876
Size: 32 6884
Tth111I GACn'n_nGTC
Cuts at: 1316 2876 1316
Size: 1560 5356
XbaI T'CTAG_A
Cuts at: 4252 4252
Size: 6916
XhoI C'TCGA_G
Cuts at: 1943 2937 1943
Size: 994 5922
XmaI C'CCGG_G
Cuts at: 1870 2505 4241 1870
Size: 635 1736 4545
Fragments arranged by size:
4545 1736 635
XmnI GAAnn'nnTTC
Cuts at: 5045 5045
Size: 6916
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3247 4724 276
Size: 53 83 186 936 1713 1477 2468
Fragments arranged by size:
2468 1713 1477 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PspOMI PspXI PsrI
PstI PvuI PvuII SacI SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoRV FseI
FspAI KasI NarI NruI PacI PmlI PpuMI RsrII
SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI