Restriction map for
pFN21AB3343
(Circular) (Six-base) MAPSORT of: pFN21AB3343.seq Check: 7737 from: 1 to: 7396
pFN21AB3343 7396 bp
With 182 enzymes: SgfI *
February 27, 2009 14:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2130 2144 3901 2130
Size: 14 1757 5625
Fragments arranged by size:
5625 1757 14
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2680 5206 278
Size: 53 83 186 936 1144 2526 2468
Fragments arranged by size:
2526 2468 1144 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2526 4739 1937
Size: 589 2213 4594
Fragments arranged by size:
4594 2213 589
Acc65I G'GTAC_C
Cuts at: 4717 4717
Size: 7396
AclI AA'CG_TT
Cuts at: 1563 5523 5896 1563
Size: 3960 373 3063
Fragments arranged by size:
3960 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2522 4655 5459 6843 7111 1632
Size: 890 2133 804 1384 268 1917
Fragments arranged by size:
2133 1917 1384 890 804 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7199
AflIII A'CryG_T
Cuts at: 1203 6295 7272 1203
Size: 5092 977 1327
Fragments arranged by size:
5092 1327 977
AgeI A'CCGG_T
Cuts at: 6227 6227
Size: 7396
AhdI GACnn_n'nnGTC
Cuts at: 3313 4651 6125 3313
Size: 1338 1474 4584
Fragments arranged by size:
4584 1474 1338
AleI CACnn'nnGTG
Cuts at: 1166 2627 1166
Size: 1461 5935
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3215 1184
Size: 2031 5365
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3560 3592 3560
Size: 32 7364
AlwNI CAG_nnn'CTG
Cuts at: 2023 2101 2746 3228 4277 5139 6711 2023
Size: 78 645 482 1049 862 1572 2708
Fragments arranged by size:
2708 1572 1049 862 645 482 78
ApaI G_GGCC'C
Cuts at: 3780 4494 3780
Size: 714 6682
ApaLI G'TGCA_C
Cuts at: 5453 6609 5453
Size: 1156 6240
ApoI r'AATT_y
Cuts at: 1493 4705 4931 5083 7192 1493
Size: 3212 226 152 2109 1697
Fragments arranged by size:
3212 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5950 160
Size: 872 4918 1606
Fragments arranged by size:
4918 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7396
AvaI C'yCGr_G
Cuts at: 1870 1943 2634 4433 4528 4721 1870
Size: 73 691 1799 95 193 4545
Fragments arranged by size:
4545 1799 691 193 95 73
BamHI G'GATC_C
Cuts at: 4726 5093 4726
Size: 367 7029
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2363 2913 4606 4717 6172
Size: 325 217 627 576 550 1693 111 1455
Cuts at: 6172 618
Size: 1842
Fragments arranged by size:
1842 1693 1455 627 576 550 325 217
111
BanII G_rGCy'C
Cuts at: 1391 2412 2696 2895 3008 3216 3780 3837 4100
Size: 1021 284 199 113 208 564 57 263
Cuts at: 4100 4270 4311 4494 4715 1391
Size: 170 41 183 221 4072
Fragments arranged by size:
4072 1021 564 284 263 221 208 199
183 170 113 57 41
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 6425
BbvCI CC'TCA_GC
Cuts at: 2882 2882
Size: 7396
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5587 5621 1903
Size: 34 3650 34 3678
Fragments arranged by size:
3678 3650 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5288 6498 5288
Size: 1210 6186
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7396
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2042 6007 136
Size: 107 122 71 631 605 370 3965 1525
Fragments arranged by size:
3965 1525 631 605 370 122 107 71
BglII A'GATC_T
Cuts at: 3682 7391 3682
Size: 3709 3687
BlpI GC'TnA_GC
Cuts at: 2777 4813 2777
Size: 2036 5360
Bme1580I G_kGCm'C
Cuts at: 1790 2283 2366 2937 3780 4494 4602 4611 5457
Size: 493 83 571 843 714 108 9 846
Cuts at: 5457 6613 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 846 843 714 571 493 108
83 9
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7396
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2870 3238 5127
Size: 416 53 83 399 24 1453 368 1889
Cuts at: 5127 6085 442
Size: 958 1753
Fragments arranged by size:
1889 1753 1453 958 416 399 368 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7396
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3316 3316
Size: 7396
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2234 2479 2531 2768 3161 3230 3298 3587
Size: 995 245 52 237 393 69 68 289
Cuts at: 3587 3654 3855 3902 4028 4140 4284 6056 1239
Size: 67 201 47 126 112 144 1772 2579
Fragments arranged by size:
2579 1772 995 393 289 245 237 201
144 126 112 69 68 67 52 47
Bpu10I CC'TnA_GC
Cuts at: 2406 2882 3561 2406
Size: 476 679 6241
Fragments arranged by size:
6241 679 476
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5521 6386 6684 5521
Size: 865 298 6233
Fragments arranged by size:
6233 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2824 3164 6059 882
Size: 617 1325 340 2895 2219
Fragments arranged by size:
2895 2219 1325 617 340
BsaAI yAC'GTr
Cuts at: 493 1204 2629 3150 5187 493
Size: 711 1425 521 2037 2702
Fragments arranged by size:
2702 2037 1425 711 521
BsaBI GATnn'nnATC
Cuts at: 7390 7390
Size: 7396
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2677 5203 5585 6220
Size: 53 83 186 936 1144 2526 382 635
Cuts at: 6220 275
Size: 1451
Fragments arranged by size:
2526 1451 1144 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4509 5829 6227 6501 6648 7059 4509
Size: 1320 398 274 147 411 4846
Fragments arranged by size:
4846 1320 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2207 2237 2852 2882 3560
Size: 30 165 30 804 30 615 30 678
Cuts at: 3560 3590 3788 3818 3875 3905 1178
Size: 30 198 30 57 30 4669
Fragments arranged by size:
4669 804 678 615 198 165 57 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2558 3443 4132 1186
Size: 1372 885 689 4450
Fragments arranged by size:
4450 1372 885 689
BseYI C'CCAG_C
Cuts at: 2052 2163 2388 2615 2681 2706 2715 2733 2843
Size: 111 225 227 66 25 9 18 110
Cuts at: 2843 3075 3446 3704 3711 3902 4229 6599 7095
Size: 232 371 258 7 191 327 2370 496
Cuts at: 7095 2052
Size: 2353
Fragments arranged by size:
2370 2353 496 371 327 258 232 227
225 191 111 110 66 25 18 9
7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6823
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4846 5607 6635 1540
Size: 275 186 2845 761 1028 2301
Fragments arranged by size:
2845 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2607 2778 2873 3008 3375 4100 4311 5457 5542
Size: 171 95 135 367 725 211 1146 85
Cuts at: 5542 6613 2607
Size: 1071 3390
Fragments arranged by size:
3390 1146 1071 725 367 211 171 135
95 85
BsmI GAATG_Cn'
Cuts at: 4907 5000 4907
Size: 93 7303
BsmBI CGTCTCn'nnnn_
Cuts at: 2971 2971
Size: 7396
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2283 2366 2412 2607 2696 2778 2873
Size: 399 493 83 46 195 89 82 95
Cuts at: 2873 2895 2937 3008 3216 3375 3780 3837 4100
Size: 22 42 71 208 159 405 57 263
Cuts at: 4100 4270 4311 4494 4602 4611 4715 5457 5542
Size: 170 41 183 108 9 104 742 85
Cuts at: 5542 6613 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 493 405 399 263 208
195 183 170 159 108 104 95 89
85 83 82 71 57 46 42 41
22 9
BspEI T'CCGG_A
Cuts at: 4509 7059 4509
Size: 2550 4846
BspHI T'CATG_A
Cuts at: 5285 7015 5285
Size: 1730 5666
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2130 2144 2427 3901 4751 844
Size: 1286 14 283 1474 850 3489
Fragments arranged by size:
3489 1474 1286 850 283 14
BsrBI CCG'CTC
Cuts at: 1394 1823 3804 5283 1394
Size: 429 1981 1479 3507
Fragments arranged by size:
3507 1981 1479 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5891 6065 65
Size: 1183 4643 174 1396
Fragments arranged by size:
4643 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3260 4326 6040 6227 1700
Size: 110 1450 1066 1714 187 2869
Fragments arranged by size:
2869 1714 1450 1066 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7396
BssHII G'CGCG_C
Cuts at: 1925 3296 1925
Size: 1371 6025
BssSI C'ACGA_G
Cuts at: 5456 6468 5456
Size: 1012 6384
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2101 2611 794
Size: 1307 510 5579
Fragments arranged by size:
5579 1307 510
BstBI TT'CG_AA
Cuts at: 6200 6200
Size: 7396
BstEII G'GTnAC_C
Cuts at: 1184 4338 1184
Size: 3154 4242
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2011
Size: 7396
BstYI r'GATC_y
Cuts at: 1972 2506 3123 3273 3435 3682 4726 5093 5478
Size: 534 617 150 162 247 1044 367 385
Cuts at: 5478 5495 6947 7033 7045 7391 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1044 617 534 385 367 346
247 162 150 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 4365 4365
Size: 7396
BtgI C'CryG_G
Cuts at: 513 1065 2004 2031 2950 3542 3957 513
Size: 552 939 27 919 592 415 3952
Fragments arranged by size:
3952 939 919 592 552 415 27
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3349 3953 487
Size: 683 56 2123 604 3930
Fragments arranged by size:
3930 2123 683 604 56
BtsI GCAGTG_nn'
Cuts at: 2917 4912 5706 5726 2917
Size: 1995 794 20 4587
Fragments arranged by size:
4587 1995 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7396
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7361
DraI TTT'AAA
Cuts at: 4700 4700
Size: 7396
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6403 809
Size: 507 5087 1802
Fragments arranged by size:
5087 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2808 2953 3144
Size: 54 1425 50 275 18 978 145 191
Cuts at: 3144 4248 4843 5732 7361 8
Size: 1104 595 889 1629 43
Fragments arranged by size:
1629 1425 1104 978 889 595 275 191
145 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4843 1537
Size: 275 3031 4090
Fragments arranged by size:
4090 3031 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2859 4628 5326 1353
Size: 1506 1769 698 3423
Fragments arranged by size:
3423 1769 1506 698
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5983 6357 6503 387
Size: 1064 138 4394 374 146 1280
Fragments arranged by size:
4394 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3006 4098 4309 3006
Size: 1092 211 6093
Fragments arranged by size:
6093 1092 211
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2234 2479 2522 2531 2768 3161 3230
Size: 393 602 245 43 9 237 393 69
Cuts at: 3230 3298 3587 3654 3855 3902 4028 4140 4284
Size: 68 289 67 201 47 126 112 144
Cuts at: 4284 4655 5459 6056 6843 7111 1239
Size: 371 804 597 787 268 1524
Fragments arranged by size:
1524 804 787 602 597 393 393 371
289 268 245 237 201 144 126 112
69 68 67 47 43 9
EcoNI CCTnn'n_nnAGG
Cuts at: 3220 4559 3220
Size: 1339 6057
EcoO109I rG'GnC_Cy
Cuts at: 2206 2416 3249 3625 3777 3876 4022 4114 4176
Size: 210 833 376 152 99 146 92 62
Cuts at: 4176 4274 4438 4490 4532 2206
Size: 98 164 52 42 5070
Fragments arranged by size:
5070 833 376 210 164 152 146 99
98 92 62 52 42
EcoRI G'AATT_C
Cuts at: 4705 5083 4705
Size: 378 7018
FspI TGC'GCA
Cuts at: 3416 5902 3416
Size: 2486 4910
HaeII r_GCGC'y
Cuts at: 1678 6543 1678
Size: 4865 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2207 2239 2321 2353 3013
Size: 32 135 32 635 32 82 32 660
Cuts at: 3013 3045 3299 3331 3635 3667 4313 4345 4637
Size: 32 254 32 304 32 646 32 292
Cuts at: 4637 4669 6037 6069 6111 6143 1373
Size: 32 1368 32 42 32 2626
Fragments arranged by size:
2626 1368 660 646 635 304 292 254
135 82 42 32 32 32 32 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2176 2329 4675 4740 4986 669
Size: 988 281 238 153 2346 65 246 3079
Fragments arranged by size:
3079 2346 988 281 246 238 153 65
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7396
HpaI GTT'AAC
Cuts at: 4986 4986
Size: 7396
KpnI G_GTAC'C
Cuts at: 4721 4721
Size: 7396
MfeI C'AATT_G
Cuts at: 4317 4995 4317
Size: 678 6718
MluI A'CGCG_T
Cuts at: 7272 7272
Size: 7396
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2368 2598 6510 6694 1305
Size: 1063 230 3912 184 2007
Fragments arranged by size:
3912 2007 1063 230 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2810 4250 10
Size: 54 1425 343 978 1440 3156
Fragments arranged by size:
3156 1440 1425 978 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2627 3020 3368 3639 5354 5713 5872
Size: 648 1461 393 348 271 1715 359 159
Cuts at: 5872 518
Size: 2042
Fragments arranged by size:
2042 1715 1461 648 393 359 348 271
159
MspA1I CmG'CkG
Cuts at: 1606 2092 2167 2274 2781 3674 4812 5489 6637
Size: 486 75 107 507 893 1138 677 1148
Cuts at: 6637 6882 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 1138 893 677 507 486 245
107 75
NcoI C'CATG_G
Cuts at: 513 1065 2004 2031 513
Size: 552 939 27 5878
Fragments arranged by size:
5878 939 552 27
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7396
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7396
NotI GC'GGCC_GC
Cuts at: 4843 4843
Size: 7396
NspI r_CATG'y
Cuts at: 1132 1229 2248 2396 3505 3638 4754 6299 1132
Size: 97 1019 148 1109 133 1116 1545 2229
Fragments arranged by size:
2229 1545 1116 1109 1019 148 133 97
PasI CC'CwG_GG
Cuts at: 1348 2017 2412 2501 2593 4391 1348
Size: 669 395 89 92 1798 4353
Fragments arranged by size:
4353 1798 669 395 92 89
PciI A'CATG_T
Cuts at: 6295 6295
Size: 7396
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2688 2746 3193 3228 3653 3940 1108
Size: 1580 58 447 35 425 287 4564
Fragments arranged by size:
4564 1580 447 425 287 58 35
PfoI T'CCnGG_A
Cuts at: 2211 2211
Size: 7396
PmeI GTTT'AAAC
Cuts at: 4700 4700
Size: 7396
PmlI CAC'GTG
Cuts at: 3150 3150
Size: 7396
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3560 3592 5465 5497 6978 7010 3560
Size: 32 1873 32 1481 32 3946
Fragments arranged by size:
3946 1873 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2206 2416 3249 3625 3876 4114 4532 2206
Size: 210 833 376 251 238 418 5070
Fragments arranged by size:
5070 833 418 376 251 238 210
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7396
PsiI TTA'TAA
Cuts at: 4966 4966
Size: 7396
PspOMI G'GGCC_C
Cuts at: 3776 4490 3776
Size: 714 6682
PstI C_TGCA'G
Cuts at: 830 2097 2141 2424 3994 4150 4455 4748 830
Size: 1267 44 283 1570 156 305 293 3478
Fragments arranged by size:
3478 1570 1267 305 293 283 156 44
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7396
PvuII CAG'CTG
Cuts at: 2781 3674 2781
Size: 893 6503
SacI G_AGCT'C
Cuts at: 3008 4100 4311 3008
Size: 1092 211 6093
Fragments arranged by size:
6093 1092 211
SalI G'TCGA_C
Cuts at: 1936 4738 1936
Size: 2802 4594
SanDI GG'GwC_CC
Cuts at: 3876 4532 3876
Size: 656 6740
SapI GCTCTTCn'nnn_
Cuts at: 4628 4628
Size: 7396
SbfI CC_TGCA'GG
Cuts at: 2141 4748 2141
Size: 2607 4789
ScaI AGT'ACT
Cuts at: 5644 7356 5644
Size: 1712 5684
SexAI A'CCwGG_T
Cuts at: 3221 4152 3221
Size: 931 6465
SfcI C'TryA_G
Cuts at: 826 1045 2093 2137 2420 3064 3582 3990 4146
Size: 219 1048 44 283 644 518 408 156
Cuts at: 4146 4451 4744 5879 6560 6751 826
Size: 305 293 1135 681 191 1471
Fragments arranged by size:
1471 1135 1048 681 644 518 408 305
293 283 219 191 156 44
SfiI GGCCn_nnn'nGGCC
Cuts at: 2042 2042
Size: 7396
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7396
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7396
SmaI CCC'GGG
Cuts at: 1872 4530 4723 1872
Size: 2658 193 4545
Fragments arranged by size:
4545 2658 193
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5500 6401 6663 820
Size: 197 926 3557 901 262 1553
Fragments arranged by size:
3557 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5187 493
Size: 4694 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7396
SphI G_CATG'C
Cuts at: 1132 4754 1132
Size: 3622 3774
SspI AAT'ATT
Cuts at: 5 52 5320 5
Size: 47 5268 2081
Fragments arranged by size:
5268 2081 47
StuI AGG'CCT
Cuts at: 1517 2356 1517
Size: 839 6557
StyI C'CwwG_G
Cuts at: 513 1065 2004 2031 2470 2978 3048 4046 4343
Size: 552 939 27 439 508 70 998 297
Cuts at: 4343 4664 4835 513
Size: 321 171 3074
Fragments arranged by size:
3074 998 939 552 508 439 321 297
171 70 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2168 2806 3182 3322 5423 5440 5593
Size: 1255 588 638 376 140 2101 17 153
Cuts at: 5593 5778 6223 325
Size: 185 445 1498
Fragments arranged by size:
2101 1498 1255 638 588 445 376 185
153 140 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3129 5642 7354
Size: 274 80 33 51 1443 1152 2513 1712
Cuts at: 7354 96
Size: 138
Fragments arranged by size:
2513 1712 1443 1152 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2480 2512 2852 2884 3265 3297 3788 3820 3875
Size: 32 340 32 381 32 491 32 55
Cuts at: 3875 3907 3942 3974 7356 7388 2480
Size: 32 35 32 3382 32 2488
Fragments arranged by size:
3382 2488 491 381 340 55 35 32
32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4536 1316
Size: 3220 4176
XbaI T'CTAG_A
Cuts at: 4732 4732
Size: 7396
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2038 2703 2038
Size: 665 6731
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7396
XmaI C'CCGG_G
Cuts at: 1870 4528 4721 1870
Size: 2658 193 4545
Fragments arranged by size:
4545 2658 193
XmnI GAAnn'nnTTC
Cuts at: 5525 5525
Size: 7396
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2678 5204 276
Size: 53 83 186 936 1144 2526 2468
Fragments arranged by size:
2526 2468 1144 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SalI SanDI SapI
SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I
DraIII EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PspXI PsrI RsrII SacII
SfoI SrfI SwaI