Restriction map for
pFN21AB3336
(Circular) (Six-base) MAPSORT of: pFN21AB3336.seq Check: 9495 from: 1 to: 6358
pFN21AB3336 6358 bp
With 182 enzymes: SgfI *
February 27, 2009 14:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2904 4168 278
Size: 53 83 186 936 1368 1264 2468
Fragments arranged by size:
2468 1368 1264 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3701 1937
Size: 1764 4594
Acc65I G'GTAC_C
Cuts at: 3679 3679
Size: 6358
AclI AA'CG_TT
Cuts at: 1563 4485 4858 1563
Size: 2922 373 3063
Fragments arranged by size:
3063 2922 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2674 2846 2858 2926 4421 5805 6073 1632
Size: 1042 172 12 68 1495 1384 268 1917
Fragments arranged by size:
1917 1495 1384 1042 268 172 68 12
AflII C'TTAA_G
Cuts at: 820 1017 3049 820
Size: 197 2032 4129
Fragments arranged by size:
4129 2032 197
AflIII A'CryG_T
Cuts at: 1203 5257 6234 1203
Size: 4054 977 1327
Fragments arranged by size:
4054 1327 977
AgeI A'CCGG_T
Cuts at: 5189 5189
Size: 6358
AhdI GACnn_n'nnGTC
Cuts at: 5087 5087
Size: 6358
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6358
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6358
AlwNI CAG_nnn'CTG
Cuts at: 2296 2683 3235 3402 3418 4101 5673 2296
Size: 387 552 167 16 683 1572 2981
Fragments arranged by size:
2981 1572 683 552 387 167 16
ApaI G_GGCC'C
Cuts at: 3577 3577
Size: 6358
ApaLI G'TGCA_C
Cuts at: 4415 5571 4415
Size: 1156 5202
ApoI r'AATT_y
Cuts at: 1493 3667 3893 4045 6154 1493
Size: 2174 226 152 2109 1697
Fragments arranged by size:
2174 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4912 160
Size: 872 3880 1606
Fragments arranged by size:
3880 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6358
AvaI C'yCGr_G
Cuts at: 1870 1943 2122 3683 1870
Size: 73 179 1561 4545
Fragments arranged by size:
4545 1561 179 73
BamHI G'GATC_C
Cuts at: 3688 4055 3688
Size: 367 5991
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2959 3031 3199 3679 5134
Size: 325 217 627 1172 72 168 480 1455
Cuts at: 5134 618
Size: 1842
Fragments arranged by size:
1842 1455 1172 627 480 325 217 168
72
BanII G_rGCy'C
Cuts at: 1391 2164 2281 2669 2885 3042 3577 3677 1391
Size: 773 117 388 216 157 535 100 4072
Fragments arranged by size:
4072 773 535 388 216 157 117 100
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3180 3204 928
Size: 971 1281 24 4082
Fragments arranged by size:
4082 1281 971 24
BbvCI CC'TCA_GC
Cuts at: 2756 2756
Size: 6358
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4549 4583 1903
Size: 34 2612 34 3678
Fragments arranged by size:
3678 2612 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4250 5460 4250
Size: 1210 5148
BclI T'GATC_A
Cuts at: 1548 2796 1548
Size: 1248 5110
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2952 3421
Size: 107 122 71 631 605 337 943 469
Cuts at: 3421 3556 4969 136
Size: 135 1413 1525
Fragments arranged by size:
1525 1413 943 631 605 469 337 135
122 107 71
BglII A'GATC_T
Cuts at: 2967 6353 2967
Size: 3386 2972
BlpI GC'TnA_GC
Cuts at: 2879 3775 2879
Size: 896 5462
Bme1580I G_kGCm'C
Cuts at: 1790 3202 3577 4419 5575 1790
Size: 1412 375 842 1156 2573
Fragments arranged by size:
2573 1412 1156 842 375
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6358
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2095 2605 3294
Size: 416 53 83 399 24 678 510 689
Cuts at: 3294 3412 4089 5047 442
Size: 118 677 958 1753
Fragments arranged by size:
1753 958 689 678 677 510 416 399
118 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6358
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2167 2519 2609 2683 2692 3008 3256 3614
Size: 928 352 90 74 9 316 248 358
Cuts at: 3614 5018 1239
Size: 1404 2579
Fragments arranged by size:
2579 1404 928 358 352 316 248 90
74 9
Bpu10I CC'TnA_GC
Cuts at: 2756 3036 3471 2756
Size: 280 435 5643
Fragments arranged by size:
5643 435 280
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2546 4483 5348 5646 2546
Size: 1937 865 298 3258
Fragments arranged by size:
3258 1937 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2429 5021 882
Size: 617 930 2592 2219
Fragments arranged by size:
2592 2219 930 617
BsaAI yAC'GTr
Cuts at: 493 1204 2431 4149 493
Size: 711 1227 1718 2702
Fragments arranged by size:
2702 1718 1227 711
BsaBI GATnn'nnATC
Cuts at: 2363 6352 2363
Size: 3989 2369
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2029 2901 3252 4165
Size: 53 83 186 936 496 872 351 913
Cuts at: 4165 4547 5182 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 913 872 635 496 382 351
186 83 53
BsaWI w'CCGG_w
Cuts at: 4791 5189 5463 5610 6021 4791
Size: 398 274 147 411 5128
Fragments arranged by size:
5128 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2886 2916 1178
Size: 30 165 30 1483 30 4620
Fragments arranged by size:
4620 1483 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2030 2039 2555 2735 2910 3296 3512 1186
Size: 844 9 516 180 175 386 216 4032
Fragments arranged by size:
4032 844 516 386 216 180 175 9
BseYI C'CCAG_C
Cuts at: 2457 2874 3317 3411 3491 5561 6057 2457
Size: 417 443 94 80 2070 496 2758
Fragments arranged by size:
2758 2070 496 443 417 94 80
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3244 1163
Size: 573 1508 4277
Fragments arranged by size:
4277 1508 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3808 4569 5597 1540
Size: 275 186 1807 761 1028 2301
Fragments arranged by size:
2301 1807 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2885 4419 4504 5575 2885
Size: 1534 85 1071 3668
Fragments arranged by size:
3668 1534 1071 85
BsmI GAATG_Cn'
Cuts at: 3869 3962 3869
Size: 93 6265
BsmBI CGTCTCn'nnnn_
Cuts at: 2585 2892 2585
Size: 307 6051
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2164 2281 2669 2885 3042 3202 3577
Size: 399 374 117 388 216 157 160 375
Cuts at: 3577 3677 4419 4504 5575 1391
Size: 100 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 388 375 374 216
160 157 117 100 85
BspEI T'CCGG_A
Cuts at: 6021 6021
Size: 6358
BspHI T'CATG_A
Cuts at: 4247 5977 4247
Size: 1730 4628
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3291 3713 844
Size: 2447 422 3489
Fragments arranged by size:
3489 2447 422
BsrBI CCG'CTC
Cuts at: 1394 1823 4245 1394
Size: 429 2422 3507
Fragments arranged by size:
3507 2422 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4853 5027 65
Size: 1183 3605 174 1396
Fragments arranged by size:
3605 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3353 5002 5189 1700
Size: 110 1543 1649 187 2869
Fragments arranged by size:
2869 1649 1543 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6358
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6358
BssSI C'ACGA_G
Cuts at: 2747 4418 5430 2747
Size: 1671 1012 3675
Fragments arranged by size:
3675 1671 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6358
BstBI TT'CG_AA
Cuts at: 5162 5162
Size: 6358
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6358
BstYI r'GATC_y
Cuts at: 1972 2580 2967 3688 4055 4440 4457 5909 5995
Size: 608 387 721 367 385 17 1452 86
Cuts at: 5995 6007 6353 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 721 608 387 385 367 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2321 2321
Size: 6358
BtgI C'CryG_G
Cuts at: 513 1065 2004 2729 2819 513
Size: 552 939 725 90 4052
Fragments arranged by size:
4052 939 725 552 90
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2362 487
Size: 683 56 1136 4483
Fragments arranged by size:
4483 1136 683 56
BtsI GCAGTG_nn'
Cuts at: 3330 3352 3874 4668 4688 3330
Size: 22 522 794 20 5000
Fragments arranged by size:
5000 794 522 22 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6358
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6323
DraI TTT'AAA
Cuts at: 3662 3662
Size: 6358
DraIII CAC_nnn'GTG
Cuts at: 3655 3655
Size: 6358
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5365 809
Size: 507 4049 1802
Fragments arranged by size:
4049 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2195 2361 2737
Size: 54 1425 50 275 18 365 166 376
Cuts at: 2737 3192 3805 4694 6323 8
Size: 455 613 889 1629 43
Fragments arranged by size:
1629 1425 889 613 455 376 365 275
166 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3805 1537
Size: 275 1993 4090
Fragments arranged by size:
4090 1993 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2923 3296 3354 4288 1353
Size: 1570 373 58 934 3423
Fragments arranged by size:
3423 1570 934 373 58
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4945 5319 5465 387
Size: 1064 138 3356 374 146 1280
Fragments arranged by size:
3356 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2883 2883
Size: 6358
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2167 2519 2609 2674 2683 2692 2846
Size: 393 535 352 90 65 9 9 154
Cuts at: 2846 2858 2926 3008 3256 3614 4421 5018 5805
Size: 12 68 82 248 358 807 597 787
Cuts at: 5805 6073 1239
Size: 268 1524
Fragments arranged by size:
1524 807 787 597 535 393 358 352
268 248 154 90 82 68 65 12
9 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2643 2714 3020 3237 3544 2643
Size: 71 306 217 307 5457
Fragments arranged by size:
5457 307 306 217 71
EcoO109I rG'GnC_Cy
Cuts at: 2545 2833 3406 3422 3502 3573 3574 3640 2545
Size: 288 573 16 80 71 1 66 5263
Fragments arranged by size:
5263 573 288 80 71 66 16 1
EcoRI G'AATT_C
Cuts at: 3667 4045 3667
Size: 378 5980
FspI TGC'GCA
Cuts at: 4864 4864
Size: 6358
HaeII r_GCGC'y
Cuts at: 1678 5505 1678
Size: 3827 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2118 2150 2886 2918 3029
Size: 32 135 32 546 32 736 32 111
Cuts at: 3029 3061 3149 3181 4999 5031 5073 5105 1373
Size: 32 88 32 1818 32 42 32 2626
Fragments arranged by size:
2626 1818 736 546 135 111 88 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2308 3702 3948 669
Size: 988 281 370 1394 246 3079
Fragments arranged by size:
3079 1394 988 370 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6358
HpaI GTT'AAC
Cuts at: 3948 3948
Size: 6358
KpnI G_GTAC'C
Cuts at: 3683 3683
Size: 6358
MfeI C'AATT_G
Cuts at: 3957 3957
Size: 6358
MluI A'CGCG_T
Cuts at: 6234 6234
Size: 6358
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5472 5656 1305
Size: 4167 184 2007
Fragments arranged by size:
4167 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2363 10
Size: 54 1425 343 531 4005
Fragments arranged by size:
4005 1425 531 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2783 4316 4675 4834 518
Size: 648 1617 1533 359 159 2042
Fragments arranged by size:
2042 1617 1533 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2084 2760 2878 3010 3075 3774 4451 5599
Size: 478 676 118 132 65 699 677 1148
Cuts at: 5599 5844 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 699 677 676 478 245 132
118 65
NcoI C'CATG_G
Cuts at: 513 1065 2004 2819 513
Size: 552 939 815 4052
Fragments arranged by size:
4052 939 815 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6358
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6358
NotI GC'GGCC_GC
Cuts at: 3805 3805
Size: 6358
NspI r_CATG'y
Cuts at: 1132 1229 2112 3716 5261 1132
Size: 97 883 1604 1545 2229
Fragments arranged by size:
2229 1604 1545 883 97
PasI CC'CwG_GG
Cuts at: 1348 3338 3418 3519 1348
Size: 1990 80 101 4187
Fragments arranged by size:
4187 1990 101 80
PciI A'CATG_T
Cuts at: 5257 5257
Size: 6358
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2683 2730 3402 3418 1108
Size: 1575 47 672 16 4048
Fragments arranged by size:
4048 1575 672 47 16
PfoI T'CCnGG_A
Cuts at: 2367 3111 2367
Size: 744 5614
PmeI GTTT'AAAC
Cuts at: 3662 3662
Size: 6358
PmlI CAC'GTG
Cuts at: 2431 2431
Size: 6358
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4427 4459 5940 5972 4427
Size: 32 1481 32 4813
Fragments arranged by size:
4813 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2833 3406 3502 3640 2833
Size: 573 96 138 5551
Fragments arranged by size:
5551 573 138 96
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6358
PsiI TTA'TAA
Cuts at: 3928 3928
Size: 6358
PspOMI G'GGCC_C
Cuts at: 3573 3573
Size: 6358
PstI C_TGCA'G
Cuts at: 830 2083 2169 2707 2807 3021 3231 3710 830
Size: 1253 86 538 100 214 210 479 3478
Fragments arranged by size:
3478 1253 538 479 214 210 100 86
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6358
PvuII CAG'CTG
Cuts at: 2878 3010 3075 2878
Size: 132 65 6161
Fragments arranged by size:
6161 132 65
SacI G_AGCT'C
Cuts at: 2885 2885
Size: 6358
SalI G'TCGA_C
Cuts at: 1936 3700 1936
Size: 1764 4594
SanDI GG'GwC_CC
Cuts at: 3406 3406
Size: 6358
SapI GCTCTTCn'nnn_
Cuts at: 3296 3296
Size: 6358
SbfI CC_TGCA'GG
Cuts at: 3710 3710
Size: 6358
ScaI AGT'ACT
Cuts at: 4606 6318 4606
Size: 1712 4646
SexAI A'CCwGG_T
Cuts at: 3447 3447
Size: 6358
SfcI C'TryA_G
Cuts at: 826 1045 2079 2165 2703 2803 3017 3227 3706
Size: 219 1034 86 538 100 214 210 479
Cuts at: 3706 4841 5522 5713 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1135 1034 681 538 479 219 214
210 191 100 86
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6358
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6358
SmaI CCC'GGG
Cuts at: 1872 3685 1872
Size: 1813 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2122 2561 3049 4462 5363 5625
Size: 197 926 179 439 488 1413 901 262
Cuts at: 5625 820
Size: 1553
Fragments arranged by size:
1553 1413 926 901 488 439 262 197
179
SnaBI TAC'GTA
Cuts at: 493 4149 493
Size: 3656 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6358
SphI G_CATG'C
Cuts at: 1132 3716 1132
Size: 2584 3774
SspI AAT'ATT
Cuts at: 5 52 4282 5
Size: 47 4230 2081
Fragments arranged by size:
4230 2081 47
StuI AGG'CCT
Cuts at: 1517 3134 3550 1517
Size: 1617 416 4325
Fragments arranged by size:
4325 1617 416
StyI C'CwwG_G
Cuts at: 513 1065 2004 2710 2819 3120 3797 513
Size: 552 939 706 109 301 677 3074
Fragments arranged by size:
3074 939 706 677 552 301 109
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2624 3217 4385 4402 4555 4740 5185
Size: 1255 1044 593 1168 17 153 185 445
Cuts at: 5185 325
Size: 1498
Fragments arranged by size:
1498 1255 1168 1044 593 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2787 3144 4604
Size: 274 80 33 51 1443 810 357 1460
Cuts at: 4604 6316 96
Size: 1712 138
Fragments arranged by size:
1712 1460 1443 810 357 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6318 6350 6318
Size: 32 6326
Tth111I GACn'n_nGTC
Cuts at: 1316 2312 2518 1316
Size: 996 206 5156
Fragments arranged by size:
5156 996 206
XbaI T'CTAG_A
Cuts at: 3694 3694
Size: 6358
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2532 2532
Size: 6358
XhoI C'TCGA_G
Cuts at: 1943 2122 1943
Size: 179 6179
XmaI C'CCGG_G
Cuts at: 1870 3683 1870
Size: 1813 4545
XmnI GAAnn'nnTTC
Cuts at: 4487 4487
Size: 6358
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2902 4166 276
Size: 53 83 186 936 1368 1264 2468
Fragments arranged by size:
2468 1368 1264 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SalI SanDI SapI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BstXI BstZ17I EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PspXI
PsrI RsrII SacII SfiI SfoI SrfI SwaI