Restriction map for
pFN21AB3334
(Circular) (Six-base) MAPSORT of: pFN21AB3334.seq Check: 4646 from: 1 to: 7015
pFN21AB3334 7015 bp
With 182 enzymes: SgfI *
February 27, 2009 14:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3732 4825 278
Size: 53 83 186 936 2196 1093 2468
Fragments arranged by size:
2468 2196 1093 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2232 4358 1937
Size: 295 2126 4594
Fragments arranged by size:
4594 2126 295
Acc65I G'GTAC_C
Cuts at: 2062 4336 2062
Size: 2274 4741
AclI AA'CG_TT
Cuts at: 1563 4314 5142 5515 1563
Size: 2751 828 373 3063
Fragments arranged by size:
3063 2751 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2595 2961 3686 4026 4193 5078 6462 6730
Size: 963 366 725 340 167 885 1384 268
Cuts at: 6730 1632
Size: 1917
Fragments arranged by size:
1917 1384 963 885 725 366 340 268
167
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6818
AflIII A'CryG_T
Cuts at: 1203 3471 5914 6891 1203
Size: 2268 2443 977 1327
Fragments arranged by size:
2443 2268 1327 977
AgeI A'CCGG_T
Cuts at: 5846 5846
Size: 7015
AhdI GACnn_n'nnGTC
Cuts at: 5744 5744
Size: 7015
AleI CACnn'nnGTG
Cuts at: 1166 2255 3606 4163 1166
Size: 1089 1351 557 4018
Fragments arranged by size:
4018 1351 1089 557
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2534 1184
Size: 1350 5665
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4035 4067 4035
Size: 32 6983
AlwNI CAG_nnn'CTG
Cuts at: 2849 3361 3971 4758 6330 2849
Size: 512 610 787 1572 3534
Fragments arranged by size:
3534 1572 787 610 512
ApaLI G'TGCA_C
Cuts at: 5072 6228 5072
Size: 1156 5859
ApoI r'AATT_y
Cuts at: 1493 2405 4025 4324 4550 4702 6811 1493
Size: 912 1620 299 226 152 2109 1697
Fragments arranged by size:
2109 1697 1620 912 299 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5569 160
Size: 872 4537 1606
Fragments arranged by size:
4537 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7015
AvaI C'yCGr_G
Cuts at: 1870 1943 2049 3078 3140 4340 1870
Size: 73 106 1029 62 1200 4545
Fragments arranged by size:
4545 1200 1029 106 73 62
BamHI G'GATC_C
Cuts at: 3559 4345 4712 3559
Size: 786 367 5862
Fragments arranged by size:
5862 786 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2062 4336 5791 618
Size: 325 217 627 275 2274 1455 1842
Fragments arranged by size:
2274 1842 1455 627 325 275 217
BanII G_rGCy'C
Cuts at: 1391 3822 4334 1391
Size: 2431 512 4072
Fragments arranged by size:
4072 2431 512
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2676 928
Size: 971 777 5267
Fragments arranged by size:
5267 971 777
BbvCI CC'TCA_GC
Cuts at: 2709 2778 2709
Size: 69 6946
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2856 2890 5206 5240 1903
Size: 34 50 34 835 34 2316 34 3678
Fragments arranged by size:
3678 2316 835 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4907 6117 4907
Size: 1210 5805
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7015
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2045 5626 136
Size: 107 122 71 631 605 373 3581 1525
Fragments arranged by size:
3581 1525 631 605 373 122 107 71
BglII A'GATC_T
Cuts at: 3999 7010 3999
Size: 3011 4004
BlpI GC'TnA_GC
Cuts at: 4432 4432
Size: 7015
Bme1580I G_kGCm'C
Cuts at: 1790 2956 5076 6232 1790
Size: 1166 2120 1156 2573
Fragments arranged by size:
2573 2120 1166 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7015
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4746 5704 442
Size: 416 53 83 399 24 3329 958 1753
Fragments arranged by size:
3329 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7015
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2635 2893 4018 2635
Size: 258 1125 5632
Fragments arranged by size:
5632 1125 258
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2078 3443 3842 5675 1239
Size: 839 1365 399 1833 2579
Fragments arranged by size:
2579 1833 1365 839 399
Bpu10I CC'TnA_GC
Cuts at: 2709 2778 2709
Size: 69 6946
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3038 3218 5140 6005 6303 3038
Size: 180 1922 865 298 3750
Fragments arranged by size:
3750 1922 865 298 180
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5678 882
Size: 617 4179 2219
Fragments arranged by size:
4179 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3472 4806 493
Size: 711 2268 1334 2702
Fragments arranged by size:
2702 2268 1334 711
BsaBI GATnn'nnATC
Cuts at: 2166 4154 7009 2166
Size: 1988 2855 2172
Fragments arranged by size:
2855 2172 1988
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3729 4221 4822 5204
Size: 53 83 186 936 2196 492 601 382
Cuts at: 5204 5839 275
Size: 635 1451
Fragments arranged by size:
2196 1451 936 635 601 492 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2985 3582 5448 5846 6120 6267 6678 2985
Size: 597 1866 398 274 147 411 3322
Fragments arranged by size:
3322 1866 597 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6790
Fragments arranged by size:
6790 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2624 2708 3447 3686 4010 4253 1186
Size: 1438 84 739 239 324 243 3948
Fragments arranged by size:
3948 1438 739 324 243 239 84
BseYI C'CCAG_C
Cuts at: 6218 6714 6218
Size: 496 6519
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6442
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3946 4465 5226 6254 1540
Size: 275 186 1945 519 761 1028 2301
Fragments arranged by size:
2301 1945 1028 761 519 275 186
BsiHKAI G_wGCw'C
Cuts at: 2879 3822 5076 5161 6232 2879
Size: 943 1254 85 1071 3662
Fragments arranged by size:
3662 1254 1071 943 85
BsmI GAATG_Cn'
Cuts at: 3868 4526 4619 3868
Size: 658 93 6264
Fragments arranged by size:
6264 658 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2885 2885
Size: 7015
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2879 2956 3822 4334 5076 5161 6232
Size: 399 1089 77 866 512 742 85 1071
Cuts at: 6232 1391
Size: 2174
Fragments arranged by size:
2174 1089 1071 866 742 512 399 85
77
BspEI T'CCGG_A
Cuts at: 6678 6678
Size: 7015
BspHI T'CATG_A
Cuts at: 3231 4904 6634 3231
Size: 1673 1730 3612
Fragments arranged by size:
3612 1730 1673
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2195 4370 844
Size: 1351 2175 3489
Fragments arranged by size:
3489 2175 1351
BsrBI CCG'CTC
Cuts at: 1394 1823 3440 3575 4902 1394
Size: 429 1617 135 1327 3507
Fragments arranged by size:
3507 1617 1327 429 135
BsrDI GCAATG_nn'
Cuts at: 65 1248 2817 2916 5510 5684 65
Size: 1183 1569 99 2594 174 1396
Fragments arranged by size:
2594 1569 1396 1183 174 99
BsrFI r'CCGG_y
Cuts at: 1700 1810 5659 5846 1700
Size: 110 3849 187 2869
Fragments arranged by size:
3849 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7015
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7015
BssSI C'ACGA_G
Cuts at: 5075 6087 5075
Size: 1012 6003
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2316 794
Size: 1522 5493
BstBI TT'CG_AA
Cuts at: 5819 5819
Size: 7015
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7015
BstYI r'GATC_y
Cuts at: 1972 2167 3082 3559 3579 3999 4345 4712 5097
Size: 195 915 477 20 420 346 367 385
Cuts at: 5097 5114 6566 6652 6664 7010 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 915 477 420 385 367 346
346 195 86 20 17 12
BtgI C'CryG_G
Cuts at: 513 1065 3813 3863 513
Size: 552 2748 50 3665
Fragments arranged by size:
3665 2748 552 50
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6276
Fragments arranged by size:
6276 683 56
BtsI GCAGTG_nn'
Cuts at: 2021 2321 2485 3669 4531 5325 5345 2021
Size: 300 164 1184 862 794 20 3691
Fragments arranged by size:
3691 1184 862 794 300 164 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7015
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2800 2835 529
Size: 35 2236 35 4709
Fragments arranged by size:
4709 2236 35 35
DraI TTT'AAA
Cuts at: 4319 4319
Size: 7015
DraIII CAC_nnn'GTG
Cuts at: 2338 2338
Size: 7015
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2624 4226 6022 809
Size: 507 1308 1602 1796 1802
Fragments arranged by size:
1802 1796 1602 1308 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2034 3018 3378
Size: 54 1425 50 275 18 204 984 360
Cuts at: 3378 3666 3735 4462 5351 6980 8
Size: 288 69 727 889 1629 43
Fragments arranged by size:
1629 1425 984 889 727 360 288 275
204 69 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4462 1537
Size: 275 2650 4090
Fragments arranged by size:
4090 2650 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2480 2766 2850 3000 3240 4945 1353
Size: 1127 286 84 150 240 1705 3423
Fragments arranged by size:
3423 1705 1127 286 240 150 84
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2652 2985 5602 5976 6122 387
Size: 1064 138 1063 333 2617 374 146 1280
Fragments arranged by size:
2617 1280 1064 1063 374 333 146 138
EcoICRI GAG'CTC
Cuts at: 3820 3820
Size: 7015
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2078 2595 2961 3443 3686 3842 4026
Size: 393 446 517 366 482 243 156 184
Cuts at: 4026 4193 5078 5675 6462 6730 1239
Size: 167 885 597 787 268 1524
Fragments arranged by size:
1524 885 787 597 517 482 446 393
366 268 243 184 167 156
EcoRI G'AATT_C
Cuts at: 4025 4324 4702 4025
Size: 299 378 6338
Fragments arranged by size:
6338 378 299
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3212 3212
Size: 7015
FspI TGC'GCA
Cuts at: 3551 5521 3551
Size: 1970 5045
FspAI rTGC'GCAy
Cuts at: 3551 3551
Size: 7015
HaeII r_GCGC'y
Cuts at: 1678 2057 3036 4246 6162 1678
Size: 379 979 1210 1916 2531
Fragments arranged by size:
2531 1916 1210 979 379
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2898 2930 3206 3238 5656
Size: 32 135 32 1326 32 276 32 2418
Cuts at: 5656 5688 5730 5762 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2418 1326 276 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3130 3978 4219 4359 4605 669
Size: 988 281 1192 848 241 140 246 3079
Fragments arranged by size:
3079 1192 988 848 281 246 241 140
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7015
HpaI GTT'AAC
Cuts at: 4605 4605
Size: 7015
KpnI G_GTAC'C
Cuts at: 2066 4340 2066
Size: 2274 4741
MfeI C'AATT_G
Cuts at: 4614 4614
Size: 7015
MluI A'CGCG_T
Cuts at: 6891 6891
Size: 7015
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4054 4307 6129 6313 1305
Size: 2749 253 1822 184 2007
Fragments arranged by size:
2749 2007 1822 253 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3380 3737 10
Size: 54 1425 343 1548 357 3288
Fragments arranged by size:
3288 1548 1425 357 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2135 2139 2255 3020 3606 4163 4203
Size: 648 969 4 116 765 586 557 40
Cuts at: 4203 4973 5332 5491 518
Size: 770 359 159 2042
Fragments arranged by size:
2042 969 770 765 648 586 557 359
159 116 40 4
MspA1I CmG'CkG
Cuts at: 1606 2352 2922 3361 4226 4431 5108 6256 6501
Size: 746 570 439 865 205 677 1148 245
Cuts at: 6501 1606
Size: 2120
Fragments arranged by size:
2120 1148 865 746 677 570 439 245
205
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6463
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7015
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7015
NotI GC'GGCC_GC
Cuts at: 4462 4462
Size: 7015
NspI r_CATG'y
Cuts at: 1132 1229 2134 2478 2515 3170 3548 4373 5918
Size: 97 905 344 37 655 378 825 1545
Cuts at: 5918 1132
Size: 2229
Fragments arranged by size:
2229 1545 905 825 655 378 344 97
37
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7015
PciI A'CATG_T
Cuts at: 5914 5914
Size: 7015
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3971 1108
Size: 2863 4152
PfoI T'CCnGG_A
Cuts at: 3309 3309
Size: 7015
PmeI GTTT'AAAC
Cuts at: 4319 4319
Size: 7015
PmlI CAC'GTG
Cuts at: 3472 3472
Size: 7015
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5084 5116 6597 6629 5084
Size: 32 1481 32 5470
Fragments arranged by size:
5470 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7015
PsiI TTA'TAA
Cuts at: 4585 4585
Size: 7015
PstI C_TGCA'G
Cuts at: 830 2217 2247 3447 3664 4367 830
Size: 1387 30 1200 217 703 3478
Fragments arranged by size:
3478 1387 1200 703 217 30
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7015
PvuII CAG'CTG
Cuts at: 3361 3361
Size: 7015
SacI G_AGCT'C
Cuts at: 3822 3822
Size: 7015
SalI G'TCGA_C
Cuts at: 1936 4357 1936
Size: 2421 4594
SbfI CC_TGCA'GG
Cuts at: 4367 4367
Size: 7015
ScaI AGT'ACT
Cuts at: 3011 5263 6975 3011
Size: 2252 1712 3051
Fragments arranged by size:
3051 2252 1712
SexAI A'CCwGG_T
Cuts at: 3964 3964
Size: 7015
SfcI C'TryA_G
Cuts at: 826 1045 2213 2243 3443 3660 4363 5498 6179
Size: 219 1168 30 1200 217 703 1135 681
Cuts at: 6179 6370 826
Size: 191 1471
Fragments arranged by size:
1471 1200 1168 1135 703 681 219 217
191 30
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7015
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7015
SmaI CCC'GGG
Cuts at: 1872 4342 1872
Size: 2470 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3053 3140 3197 5119 6020 6282
Size: 197 926 1110 87 57 1922 901 262
Cuts at: 6282 820
Size: 1553
Fragments arranged by size:
1922 1553 1110 926 901 262 197 87
57
SnaBI TAC'GTA
Cuts at: 493 4806 493
Size: 4313 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7015
SphI G_CATG'C
Cuts at: 1132 4373 1132
Size: 3241 3774
SspI AAT'ATT
Cuts at: 5 52 4939 5
Size: 47 4887 2081
Fragments arranged by size:
4887 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7015
StyI C'CwwG_G
Cuts at: 513 1065 2199 3755 4161 4454 513
Size: 552 1134 1556 406 293 3074
Fragments arranged by size:
3074 1556 1134 552 406 293
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3812 3960 5042 5059 5212 5397 5842
Size: 1255 2232 148 1082 17 153 185 445
Cuts at: 5842 325
Size: 1498
Fragments arranged by size:
2232 1498 1255 1082 445 185 153 148
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3009 4185 5261
Size: 274 80 33 51 1443 1032 1176 1076
Cuts at: 5261 6973 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1176 1076 1032 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2615 2647 4107 4139 6975 7007 2615
Size: 32 1460 32 2836 32 2623
Fragments arranged by size:
2836 2623 1460 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7015
XbaI T'CTAG_A
Cuts at: 4351 4351
Size: 7015
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2848 2848
Size: 7015
XhoI C'TCGA_G
Cuts at: 1943 3140 1943
Size: 1197 5818
XmaI C'CCGG_G
Cuts at: 1870 4340 1870
Size: 2470 4545
XmnI GAAnn'nnTTC
Cuts at: 5144 5144
Size: 7015
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3730 4823 276
Size: 53 83 186 936 2196 1093 2468
Fragments arranged by size:
2468 2196 1093 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FalI
FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PshAI PsiI PstI PvuI PvuII SacI
SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BfrBI
BsiWI BstXI BstZ17I Bsu36I EcoNI EcoO109I EcoRV FseI
KasI NaeI NarI NgoMIV NruI NsiI PacI PpuMI
PspOMI PspXI PsrI RsrII SacII SanDI SapI SfiI
SfoI SrfI SwaI