Restriction map for
pFN21AB3332
(Circular) (Six-base) MAPSORT of: pFN21AB3332.seq Check: 215 from: 1 to: 8095
pFN21AB3332 8095 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2404 3396 3944 3955 5260 2404
Size: 992 548 11 1305 5239
Fragments arranged by size:
5239 1305 992 548 11
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5905 278
Size: 53 83 186 936 4369 2468
Fragments arranged by size:
4369 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5438 1937
Size: 3501 4594
Acc65I G'GTAC_C
Cuts at: 5416 5416
Size: 8095
AclI AA'CG_TT
Cuts at: 1563 6222 6595 1563
Size: 4659 373 3063
Fragments arranged by size:
4659 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2786 3335 3967 4041 4512 4611 5109 6158
Size: 1154 549 632 74 471 99 498 1049
Cuts at: 6158 7542 7810 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1384 1154 1049 632 549 498 471
268 99 74
AfeI AGC'GCT
Cuts at: 5094 5094
Size: 8095
AflII C'TTAA_G
Cuts at: 820 1017 3422 820
Size: 197 2405 5493
Fragments arranged by size:
5493 2405 197
AflIII A'CryG_T
Cuts at: 1203 3564 6994 7971 1203
Size: 2361 3430 977 1327
Fragments arranged by size:
3430 2361 1327 977
AgeI A'CCGG_T
Cuts at: 6926 6926
Size: 8095
AhdI GACnn_n'nnGTC
Cuts at: 2070 3142 6824 2070
Size: 1072 3682 3341
Fragments arranged by size:
3682 3341 1072
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8095
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8095
AlwNI CAG_nnn'CTG
Cuts at: 2855 4085 5387 5838 7410 2855
Size: 1230 1302 451 1572 3540
Fragments arranged by size:
3540 1572 1302 1230 451
ApaI G_GGCC'C
Cuts at: 2031 2096 2251 2836 3932 3991 4013 5148 2031
Size: 65 155 585 1096 59 22 1135 4978
Fragments arranged by size:
4978 1135 1096 585 155 65 59 22
ApaLI G'TGCA_C
Cuts at: 4929 6152 7308 4929
Size: 1223 1156 5716
Fragments arranged by size:
5716 1223 1156
ApoI r'AATT_y
Cuts at: 1493 4805 5404 5630 5782 7891 1493
Size: 3312 599 226 152 2109 1697
Fragments arranged by size:
3312 2109 1697 599 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4647 6649 160
Size: 872 3615 2002 1606
Fragments arranged by size:
3615 2002 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8095
AvaI C'yCGr_G
Cuts at: 1870 1943 2089 2204 2625 3903 4379 5012 5420
Size: 73 146 115 421 1278 476 633 408
Cuts at: 5420 1870
Size: 4545
Fragments arranged by size:
4545 1278 633 476 421 408 146 115
73
AvrII C'CTAG_G
Cuts at: 2968 3697 4129 2968
Size: 729 432 6934
Fragments arranged by size:
6934 729 432
BamHI G'GATC_C
Cuts at: 3189 5425 5792 3189
Size: 2236 367 5492
Fragments arranged by size:
5492 2236 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4418 5416 6871 618
Size: 325 217 627 2631 998 1455 1842
Fragments arranged by size:
2631 1842 1455 998 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2031 2042 2090 2096 2211 2251 2363 2693
Size: 640 11 48 6 115 40 112 330
Cuts at: 2693 2836 2976 3001 3449 3722 3932 3991 4013
Size: 143 140 25 448 273 210 59 22
Cuts at: 4013 4031 5148 5178 5414 1391
Size: 18 1117 30 236 4072
Fragments arranged by size:
4072 1117 640 448 330 273 236 210
143 140 115 112 59 48 40 30
25 22 18 11 6
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4734 4893 928
Size: 971 2835 159 4130
Fragments arranged by size:
4130 2835 971 159
BbvCI CC'TCA_GC
Cuts at: 2694 3050 3590 4039 4075 2694
Size: 356 540 449 36 6714
Fragments arranged by size:
6714 540 449 356 36
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2070 2104 3551 3585 6286 6320 1903
Size: 34 133 34 1447 34 2701 34 3678
Fragments arranged by size:
3678 2701 1447 133 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5987 7197 5987
Size: 1210 6885
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8095
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 4384 5330
Size: 107 122 71 631 605 337 2375 946
Cuts at: 5330 6706 136
Size: 1376 1525
Fragments arranged by size:
2375 1525 1376 946 631 605 337 122
107 71
BglII A'GATC_T
Cuts at: 4335 8090 4335
Size: 3755 4340
BlpI GC'TnA_GC
Cuts at: 2981 3392 5512 2981
Size: 411 2120 5564
Fragments arranged by size:
5564 2120 411
Bme1580I G_kGCm'C
Cuts at: 1790 2031 2096 2251 2836 3808 3932 3991 4013
Size: 241 65 155 585 972 124 59 22
Cuts at: 4013 4092 4287 4380 4933 5148 6156 7312 1790
Size: 79 195 93 553 215 1008 1156 2573
Fragments arranged by size:
2573 1156 1008 972 585 553 241 215
195 155 124 93 79 65 59 22
BmgBI CAC'GTC
Cuts at: 1318 2297 3565 1318
Size: 979 1268 5848
Fragments arranged by size:
5848 1268 979
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3477 4455 4573
Size: 416 53 83 399 24 2060 978 118
Cuts at: 4573 5826 6784 442
Size: 1253 958 1753
Fragments arranged by size:
2060 1753 1253 978 958 416 399 118
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8095
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3266 3871 5407 3266
Size: 605 1536 5954
Fragments arranged by size:
5954 1536 605
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2138 2915 3240 3250 3387 3936 4913 4938
Size: 899 777 325 10 137 549 977 25
Cuts at: 4938 5279 6755 1239
Size: 341 1476 2579
Fragments arranged by size:
2579 1476 977 899 777 549 341 325
137 25 10
Bpu10I CC'TnA_GC
Cuts at: 2694 3050 3590 4039 4075 2694
Size: 356 540 449 36 6714
Fragments arranged by size:
6714 540 449 356 36
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2707 3146 3866 6220 7085 7383 2707
Size: 439 720 2354 865 298 3419
Fragments arranged by size:
3419 2354 865 720 439 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2078 3856 6758 882
Size: 617 579 1778 2902 2219
Fragments arranged by size:
2902 2219 1778 617 579
BsaAI yAC'GTr
Cuts at: 493 1204 5886 493
Size: 711 4682 2702
Fragments arranged by size:
4682 2702 711
BsaBI GATnn'nnATC
Cuts at: 3194 8089 3194
Size: 4895 3200
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5902 6284 6919 275
Size: 53 83 186 936 4369 382 635 1451
Fragments arranged by size:
4369 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2167 3534 6528 6926 7200 7347 7758 2167
Size: 1367 2994 398 274 147 411 2504
Fragments arranged by size:
2994 2504 1367 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2988 3018 3223 3253 3704
Size: 30 165 30 1585 30 205 30 451
Cuts at: 3704 3734 3934 3964 4555 4585 4902 4932 4936
Size: 30 200 30 591 30 317 30 4
Cuts at: 4936 4966 1178
Size: 30 4307
Fragments arranged by size:
4307 1585 591 451 317 205 200 165
30 30 30 30 30 30 30 30
30 4
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2468 3372 3644 3768 3783 3844 4331 4499
Size: 1282 904 272 124 15 61 487 168
Cuts at: 4499 4685 4688 4736 4796 4862 4871 4874 5048
Size: 186 3 48 60 66 9 3 174
Cuts at: 5048 5063 5066 5219 1186
Size: 15 3 153 4062
Fragments arranged by size:
4062 1282 904 487 272 186 174 168
153 124 66 61 60 48 15 15
9 3 3 3
BseYI C'CCAG_C
Cuts at: 2048 2493 2557 2781 2827 4618 7298 7794 2048
Size: 445 64 224 46 1791 2680 496 2349
Fragments arranged by size:
2680 2349 1791 496 445 224 64 46
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2487 2801 2885 3038 3132 3341 3561
Size: 573 751 314 84 153 94 209 220
Cuts at: 3561 5009 5252 1163
Size: 1448 243 4006
Fragments arranged by size:
4006 1448 751 573 314 243 220 209
153 94 84
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3202 5545 6306 7334 1540
Size: 275 186 1201 2343 761 1028 2301
Fragments arranged by size:
2343 2301 1201 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2090 3001 3722 4933 6156 6241 7312 2090
Size: 911 721 1211 1223 85 1071 2873
Fragments arranged by size:
2873 1223 1211 1071 911 721 85
BsmI GAATG_Cn'
Cuts at: 2406 3351 5606 5699 2406
Size: 945 2255 93 4802
Fragments arranged by size:
4802 2255 945 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2303 3780 2303
Size: 1477 6618
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2031 2042 2090 2096 2211 2251 2363
Size: 399 241 11 48 6 115 40 112
Cuts at: 2363 2693 2836 2976 3001 3449 3722 3808 3932
Size: 330 143 140 25 448 273 86 124
Cuts at: 3932 3991 4013 4031 4092 4287 4380 4933 5148
Size: 59 22 18 61 195 93 553 215
Cuts at: 5148 5178 5414 6156 6241 7312 1391
Size: 30 236 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 553 448 399 330 273
241 236 215 195 143 140 124 115
112 93 86 85 61 59 48 40
30 25 22 18 11 6
BspEI T'CCGG_A
Cuts at: 7758 7758
Size: 8095
BspHI T'CATG_A
Cuts at: 5984 7714 5984
Size: 1730 6365
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2404 3147 3313 3396 3944 3955 5260 5450
Size: 1560 743 166 83 548 11 1305 190
Cuts at: 5450 844
Size: 3489
Fragments arranged by size:
3489 1560 1305 743 548 190 166 83
11
BsrBI CCG'CTC
Cuts at: 1394 1823 2187 5982 1394
Size: 429 364 3795 3507
Fragments arranged by size:
3795 3507 429 364
BsrDI GCAATG_nn'
Cuts at: 65 1248 3556 6590 6764 65
Size: 1183 2308 3034 174 1396
Fragments arranged by size:
3034 2308 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3171 6739 6926 1700
Size: 110 1361 3568 187 2869
Fragments arranged by size:
3568 2869 1361 187 110
BsrGI T'GTAC_A
Cuts at: 96 3679 96
Size: 3583 4512
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8095
BssSI C'ACGA_G
Cuts at: 3715 6155 7167 3715
Size: 2440 1012 4643
Fragments arranged by size:
4643 2440 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2824 2855 794
Size: 2030 31 6034
Fragments arranged by size:
6034 2030 31
BstBI TT'CG_AA
Cuts at: 6899 6899
Size: 8095
BstEII G'GTnAC_C
Cuts at: 1184 3877 1184
Size: 2693 5402
BstXI CCAn_nnnn'nTGG
Cuts at: 2537 2537
Size: 8095
BstYI r'GATC_y
Cuts at: 1972 3189 4335 5425 5792 6177 6194 7646 7732
Size: 1217 1146 1090 367 385 17 1452 86
Cuts at: 7732 7744 8090 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1217 1146 1090 385 367 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2468 2564 3966 4414 2468
Size: 96 1402 448 6149
Fragments arranged by size:
6149 1402 448 96
BtgI C'CryG_G
Cuts at: 513 1065 2004 2043 2096 2457 3437 3924 513
Size: 552 939 39 53 361 980 487 4684
Fragments arranged by size:
4684 980 939 552 487 361 53 39
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3960 487
Size: 683 56 2734 4622
Fragments arranged by size:
4622 2734 683 56
BtsI GCAGTG_nn'
Cuts at: 2088 2813 4666 5611 6405 6425 2088
Size: 725 1853 945 794 20 3758
Fragments arranged by size:
3758 1853 945 794 725 20
ClaI AT'CG_AT
Cuts at: 1554 5343 1554
Size: 3789 4306
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4563 4598 529
Size: 35 3999 35 4026
Fragments arranged by size:
4026 3999 35 35
DraI TTT'AAA
Cuts at: 5399 5399
Size: 8095
DraIII CAC_nnn'GTG
Cuts at: 2389 2389
Size: 8095
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7102 809
Size: 507 5786 1802
Fragments arranged by size:
5786 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2454 3199 3826
Size: 54 1425 50 275 18 624 745 627
Cuts at: 3826 5331 5542 6431 8060 8
Size: 1505 211 889 1629 43
Fragments arranged by size:
1629 1505 1425 889 745 627 624 275
211 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3199 5542 1537
Size: 275 1387 2343 4090
Fragments arranged by size:
4090 2343 1387 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4623 4662 4674 4683 4698 4707 4713 4734
Size: 3270 39 12 9 15 9 6 21
Cuts at: 4734 4740 4749 4764 4773 4785 4839 4872 5166
Size: 6 9 15 9 12 54 33 294
Cuts at: 5166 5196 5256 6025 1353
Size: 30 60 769 3423
Fragments arranged by size:
3423 3270 769 294 60 54 39 33
30 21 15 15 12 12 9 9
9 9 6 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2093 4527 6682 7056 7202 387
Size: 1064 138 504 2434 2155 374 146 1280
Fragments arranged by size:
2434 2155 1280 1064 504 374 146 138
EcoICRI GAG'CTC
Cuts at: 2088 2999 3720 2088
Size: 911 721 6463
Fragments arranged by size:
6463 911 721
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2138 2786 2915 3240 3250 3335 3387
Size: 393 506 648 129 325 10 85 52
Cuts at: 3387 3936 3967 4041 4512 4611 4913 4938 5109
Size: 549 31 74 471 99 302 25 171
Cuts at: 5109 5279 6158 6755 7542 7810 1239
Size: 170 879 597 787 268 1524
Fragments arranged by size:
1524 879 787 648 597 549 506 471
393 325 302 268 171 170 129 99
85 74 52 31 25 10
EcoNI CCTnn'n_nnAGG
Cuts at: 2840 4059 4628 5139 2840
Size: 1219 569 511 5796
Fragments arranged by size:
5796 1219 569 511
EcoO109I rG'GnC_Cy
Cuts at: 2028 2247 2326 2431 2473 2832 2833 2876 3217
Size: 219 79 105 42 359 1 43 341
Cuts at: 3217 3333 3373 3694 3929 3970 3987 3988 4010
Size: 116 40 321 235 41 17 1 22
Cuts at: 4010 4046 4064 4082 4213 4514 4613 5144 5145
Size: 36 18 18 131 301 99 531 1
Cuts at: 5145 2028
Size: 4978
Fragments arranged by size:
4978 531 359 341 321 301 235 219
131 116 105 99 79 43 42 41
40 36 22 18 18 17 1 1
1
EcoRI G'AATT_C
Cuts at: 5404 5782 5404
Size: 378 7717
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3860 3860
Size: 8095
FspI TGC'GCA
Cuts at: 6601 6601
Size: 8095
HaeII r_GCGC'y
Cuts at: 1678 3483 3761 5096 7242 1678
Size: 1805 278 1335 2146 2531
Fragments arranged by size:
2531 2146 1805 1335 278
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2056 2088 3128 3160 3236
Size: 32 135 32 484 32 1040 32 76
Cuts at: 3236 3268 3959 3991 4307 4339 4555 4587 6736
Size: 32 691 32 316 32 216 32 2149
Cuts at: 6736 6768 6810 6842 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2149 1040 691 484 316 216 135
76 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5439 5685 669
Size: 988 281 3501 246 3079
Fragments arranged by size:
3501 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8095
HpaI GTT'AAC
Cuts at: 5685 5685
Size: 8095
KpnI G_GTAC'C
Cuts at: 5420 5420
Size: 8095
MfeI C'AATT_G
Cuts at: 5694 5694
Size: 8095
MluI A'CGCG_T
Cuts at: 7971 7971
Size: 8095
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2725 5178 7209 7393 1305
Size: 1420 2453 2031 184 2007
Fragments arranged by size:
2453 2031 2007 1420 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 6273
Fragments arranged by size:
6273 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2522 3084 3605 6053 6412 6571 518
Size: 648 1356 562 521 2448 359 159 2042
Fragments arranged by size:
2448 2042 1356 648 562 521 359 159
MspA1I CmG'CkG
Cuts at: 1606 2045 2098 2497 2964 3413 3439 3726 5321
Size: 439 53 399 467 449 26 287 1595
Cuts at: 5321 5511 6188 7336 7581 1606
Size: 190 677 1148 245 2120
Fragments arranged by size:
2120 1595 1148 677 467 449 439 399
287 245 190 53 26
NaeI GCC'GGC
Cuts at: 3173 3173
Size: 8095
NcoI C'CATG_G
Cuts at: 513 1065 2004 3924 513
Size: 552 939 1920 4684
Fragments arranged by size:
4684 1920 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8095
NgoMIV G'CCGG_C
Cuts at: 3171 3171
Size: 8095
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8095
NotI GC'GGCC_GC
Cuts at: 5542 5542
Size: 8095
NspI r_CATG'y
Cuts at: 1132 1229 5453 6998 1132
Size: 97 4224 1545 2229
Fragments arranged by size:
4224 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2879 4511 5104 1348
Size: 1531 1632 593 4339
Fragments arranged by size:
4339 1632 1531 593
PciI A'CATG_T
Cuts at: 6994 6994
Size: 8095
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2707 1108
Size: 1599 6496
PfoI T'CCnGG_A
Cuts at: 2511 3027 3227 5295 2511
Size: 516 200 2068 5311
Fragments arranged by size:
5311 2068 516 200
PmeI GTTT'AAAC
Cuts at: 5399 5399
Size: 8095
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6164 6196 7677 7709 6164
Size: 32 1481 32 6550
Fragments arranged by size:
6550 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2431 2473 3217 3694 4213 4613 2431
Size: 42 744 477 519 400 5913
Fragments arranged by size:
5913 744 519 477 400 42
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8095
PsiI TTA'TAA
Cuts at: 5665 5665
Size: 8095
PspOMI G'GGCC_C
Cuts at: 2027 2092 2247 2832 3928 3987 4009 5144 2027
Size: 65 155 585 1096 59 22 1135 4978
Fragments arranged by size:
4978 1135 1096 585 155 65 59 22
PstI C_TGCA'G
Cuts at: 830 3921 4507 5257 5447 830
Size: 3091 586 750 190 3478
Fragments arranged by size:
3478 3091 750 586 190
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8095
PvuII CAG'CTG
Cuts at: 2497 3413 5321 2497
Size: 916 1908 5271
Fragments arranged by size:
5271 1908 916
SacI G_AGCT'C
Cuts at: 2090 3001 3722 2090
Size: 911 721 6463
Fragments arranged by size:
6463 911 721
SacII CC_GC'GG
Cuts at: 2046 2099 3440 2046
Size: 53 1341 6701
Fragments arranged by size:
6701 1341 53
SalI G'TCGA_C
Cuts at: 1936 5437 1936
Size: 3501 4594
SapI GCTCTTCn'nnn_
Cuts at: 5166 5166
Size: 8095
SbfI CC_TGCA'GG
Cuts at: 5447 5447
Size: 8095
ScaI AGT'ACT
Cuts at: 6343 8055 6343
Size: 1712 6383
SexAI A'CCwGG_T
Cuts at: 3654 3654
Size: 8095
SfcI C'TryA_G
Cuts at: 826 1045 3917 4434 4503 5253 5443 6578 7259
Size: 219 2872 517 69 750 190 1135 681
Cuts at: 7259 7450 826
Size: 191 1471
Fragments arranged by size:
2872 1471 1135 750 681 517 219 191
190 69
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8095
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8095
SmaI CCC'GGG
Cuts at: 1872 2206 3905 5014 5422 1872
Size: 334 1699 1109 408 4545
Fragments arranged by size:
4545 1699 1109 408 334
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2686 3125 3422 3845 6199 7100
Size: 197 926 743 439 297 423 2354 901
Cuts at: 7100 7362 820
Size: 262 1553
Fragments arranged by size:
2354 1553 926 901 743 439 423 297
262 197
SnaBI TAC'GTA
Cuts at: 493 5886 493
Size: 5393 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8095
SphI G_CATG'C
Cuts at: 1132 5453 1132
Size: 4321 3774
SrfI GCCC'GGGC
Cuts at: 2206 2206
Size: 8095
SspI AAT'ATT
Cuts at: 5 52 3070 4763 6019 5
Size: 47 3018 1693 1256 2081
Fragments arranged by size:
3018 2081 1693 1256 47
StuI AGG'CCT
Cuts at: 1517 2763 3131 3271 3588 4134 4149 1517
Size: 1246 368 140 317 546 15 5463
Fragments arranged by size:
5463 1246 546 368 317 140 15
StyI C'CwwG_G
Cuts at: 513 1065 2004 2758 2968 3132 3291 3697 3924
Size: 552 939 754 210 164 159 406 227
Cuts at: 3924 4129 4341 5534 513
Size: 205 212 1193 3074
Fragments arranged by size:
3074 1193 939 754 552 406 227 212
210 205 164 159
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3307 3398 3665 6122 6139 6292 6477
Size: 1255 1727 91 267 2457 17 153 185
Cuts at: 6477 6922 325
Size: 445 1498
Fragments arranged by size:
2457 1727 1498 1255 445 267 185 153
91 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3679 6341 8053
Size: 274 80 33 51 1443 1702 2662 1712
Cuts at: 8053 96
Size: 138
Fragments arranged by size:
2662 1712 1702 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2988 3020 3934 3966 4588 4620 8055 8087 2988
Size: 32 914 32 622 32 3435 32 2996
Fragments arranged by size:
3435 2996 914 622 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3656 1316
Size: 2340 5755
XbaI T'CTAG_A
Cuts at: 5431 5431
Size: 8095
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2449 4021 2449
Size: 1572 6523
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8095
XmaI C'CCGG_G
Cuts at: 1870 2204 3903 5012 5420 1870
Size: 334 1699 1109 408 4545
Fragments arranged by size:
4545 1699 1109 408 334
XmnI GAAnn'nnTTC
Cuts at: 4497 6224 4497
Size: 1727 6368
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5903 276
Size: 53 83 186 936 4369 2468
Fragments arranged by size:
4369 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SacII SalI SapI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI BaeI BbeI BfrBI BsiWI BstZ17I EcoRV
FseI FspAI KasI NarI NruI NsiI PacI PmlI
PspXI PsrI RsrII SanDI SfiI SfoI SwaI