Restriction map for
pFN21AB3331
(Circular) (Six-base) MAPSORT of: pFN21AB3331.seq Check: 2381 from: 1 to: 6247
pFN21AB3331 6247 bp
With 182 enzymes: SgfI *
October 20, 2009 10:53 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4057 278
Size: 53 83 186 936 2521 2468
Fragments arranged by size:
2521 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3468 3590 1937
Size: 1531 122 4594
Fragments arranged by size:
4594 1531 122
Acc65I G'GTAC_C
Cuts at: 3568 3568
Size: 6247
AclI AA'CG_TT
Cuts at: 1563 4374 4747 1563
Size: 2811 373 3063
Fragments arranged by size:
3063 2811 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3465 4310 5694 5962 1632
Size: 1833 845 1384 268 1917
Fragments arranged by size:
1917 1833 1384 845 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6050
AflIII A'CryG_T
Cuts at: 1203 2073 5146 6123 1203
Size: 870 3073 977 1327
Fragments arranged by size:
3073 1327 977 870
AgeI A'CCGG_T
Cuts at: 5078 5078
Size: 6247
AhdI GACnn_n'nnGTC
Cuts at: 4976 4976
Size: 6247
AleI CACnn'nnGTG
Cuts at: 1166 2042 1166
Size: 876 5371
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6247
AlwNI CAG_nnn'CTG
Cuts at: 2315 3990 5562 2315
Size: 1675 1572 3000
Fragments arranged by size:
3000 1675 1572
ApaLI G'TGCA_C
Cuts at: 4304 5460 4304
Size: 1156 5091
ApoI r'AATT_y
Cuts at: 1493 2491 3200 3268 3556 3782 3934 6043 1493
Size: 998 709 68 288 226 152 2109 1697
Fragments arranged by size:
2109 1697 998 709 288 226 152 68
AseI AT'TA_AT
Cuts at: 160 1032 4801 160
Size: 872 3769 1606
Fragments arranged by size:
3769 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6247
AvaI C'yCGr_G
Cuts at: 1870 1943 3572 1870
Size: 73 1629 4545
Fragments arranged by size:
4545 1629 73
BamHI G'GATC_C
Cuts at: 3577 3944 3577
Size: 367 5880
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2574 3305 3568 5023 618
Size: 325 217 627 787 731 263 1455 1842
Fragments arranged by size:
1842 1455 787 731 627 325 263 217
BanII G_rGCy'C
Cuts at: 1391 2780 3566 1391
Size: 1389 786 4072
Fragments arranged by size:
4072 1389 786
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2808 928
Size: 971 909 4367
Fragments arranged by size:
4367 971 909
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4438 4472 1903
Size: 34 2501 34 3678
Fragments arranged by size:
3678 2501 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4139 5349 4139
Size: 1210 5037
BclI T'GATC_A
Cuts at: 1548 3415 1548
Size: 1867 4380
BfrBI ATG'CAT
Cuts at: 3313 3313
Size: 6247
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4858 136
Size: 107 122 71 631 605 3186 1525
Fragments arranged by size:
3186 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2691 6242 2691
Size: 3551 2696
BlpI GC'TnA_GC
Cuts at: 3664 3664
Size: 6247
Bme1580I G_kGCm'C
Cuts at: 1790 4308 5464 1790
Size: 2518 1156 2573
Fragments arranged by size:
2573 2518 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6247
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2563 2742 3978
Size: 416 53 83 399 24 1146 179 1236
Cuts at: 3978 4936 442
Size: 958 1753
Fragments arranged by size:
1753 1236 1146 958 416 399 179 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6247
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3162 3312 3346 4907 1239
Size: 1923 150 34 1561 2579
Fragments arranged by size:
2579 1923 1561 150 34
Bpu10I CC'TnA_GC
Cuts at: 2380 2380
Size: 6247
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4372 5237 5535 4372
Size: 865 298 5084
Fragments arranged by size:
5084 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4910 882
Size: 617 3411 2219
Fragments arranged by size:
3411 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4038 493
Size: 711 2834 2702
Fragments arranged by size:
2834 2702 711
BsaBI GATnn'nnATC
Cuts at: 2418 6241 2418
Size: 3823 2424
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4054 4436 5071 275
Size: 53 83 186 936 2521 382 635 1451
Fragments arranged by size:
2521 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3233 4680 5078 5352 5499 5910 3233
Size: 1447 398 274 147 411 3570
Fragments arranged by size:
3570 1447 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3270 3300 1178
Size: 30 165 30 1867 30 4125
Fragments arranged by size:
4125 1867 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2027 1186
Size: 841 5406
BseYI C'CCAG_C
Cuts at: 2619 2820 5450 5946 2619
Size: 201 2630 496 2920
Fragments arranged by size:
2920 2630 496 201
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2355 2514 2892 2922 1163
Size: 573 619 159 378 30 4488
Fragments arranged by size:
4488 619 573 378 159 30
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3697 4458 5486 1540
Size: 275 186 1696 761 1028 2301
Fragments arranged by size:
2301 1696 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4308 4393 5464 4308
Size: 85 1071 5091
Fragments arranged by size:
5091 1071 85
BsmI GAATG_Cn'
Cuts at: 2224 2358 3758 3851 2224
Size: 134 1400 93 4620
Fragments arranged by size:
4620 1400 134 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2780 3566 4308 4393 5464 1391
Size: 399 990 786 742 85 1071 2174
Fragments arranged by size:
2174 1071 990 786 742 399 85
BspEI T'CCGG_A
Cuts at: 5910 5910
Size: 6247
BspHI T'CATG_A
Cuts at: 4136 5866 4136
Size: 1730 4517
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2279 3602 844
Size: 1435 1323 3489
Fragments arranged by size:
3489 1435 1323
BsrBI CCG'CTC
Cuts at: 1394 1823 4134 1394
Size: 429 2311 3507
Fragments arranged by size:
3507 2311 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4742 4916 65
Size: 1183 3494 174 1396
Fragments arranged by size:
3494 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4891 5078 1700
Size: 110 3081 187 2869
Fragments arranged by size:
3081 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2032 2971 96
Size: 1936 939 3372
Fragments arranged by size:
3372 1936 939
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6247
BssSI C'ACGA_G
Cuts at: 4307 5319 4307
Size: 1012 5235
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6247
BstBI TT'CG_AA
Cuts at: 5051 5051
Size: 6247
BstEII G'GTnAC_C
Cuts at: 1184 2914 1184
Size: 1730 4517
BstXI CCAn_nnnn'nTGG
Cuts at: 3513 3513
Size: 6247
BstYI r'GATC_y
Cuts at: 1972 2343 2669 2691 2824 3076 3577 3944 4329
Size: 371 326 22 133 252 501 367 385
Cuts at: 4329 4346 5798 5884 5896 6242 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 501 385 371 367 346 326
252 133 86 22 17 12
BstZ17I GTA'TAC
Cuts at: 3469 3469
Size: 6247
BtgI C'CryG_G
Cuts at: 513 1065 2004 2582 513
Size: 552 939 578 4178
Fragments arranged by size:
4178 939 578 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2501 487
Size: 683 56 1275 4233
Fragments arranged by size:
4233 1275 683 56
BtsI GCAGTG_nn'
Cuts at: 2880 3200 3763 4557 4577 2880
Size: 320 563 794 20 4550
Fragments arranged by size:
4550 794 563 320 20
ClaI AT'CG_AT
Cuts at: 1554 2904 1554
Size: 1350 4897
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6212
DraI TTT'AAA
Cuts at: 3551 3551
Size: 6247
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5254 809
Size: 507 3938 1802
Fragments arranged by size:
3938 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3694 4583 6212
Size: 54 1425 50 275 18 1864 889 1629
Cuts at: 6212 8
Size: 43
Fragments arranged by size:
1864 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3694 1537
Size: 275 1882 4090
Fragments arranged by size:
4090 1882 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2054 2109 4177 1353
Size: 701 55 2068 3423
Fragments arranged by size:
3423 2068 701 55
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4834 5208 5354 387
Size: 1064 138 3245 374 146 1280
Fragments arranged by size:
3245 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3162 3312 3346 3465 4310 4907 5694
Size: 393 1530 150 34 119 845 597 787
Cuts at: 5694 5962 1239
Size: 268 1524
Fragments arranged by size:
1530 1524 845 787 597 393 268 150
119 34
EcoO109I rG'GnC_Cy
Cuts at: 2064 2210 2312 2064
Size: 146 102 5999
Fragments arranged by size:
5999 146 102
EcoRI G'AATT_C
Cuts at: 2491 3556 3934 2491
Size: 1065 378 4804
Fragments arranged by size:
4804 1065 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2213 2213
Size: 6247
FspI TGC'GCA
Cuts at: 4753 4753
Size: 6247
HaeII r_GCGC'y
Cuts at: 1678 5394 1678
Size: 3716 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2767 2799 3374 3406 4888
Size: 32 135 32 1195 32 575 32 1482
Cuts at: 4888 4920 4962 4994 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1482 1195 575 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3591 3837 669
Size: 988 281 1653 246 3079
Fragments arranged by size:
3079 1653 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2144 748
Size: 1396 4851
HpaI GTT'AAC
Cuts at: 3837 3837
Size: 6247
KpnI G_GTAC'C
Cuts at: 3572 3572
Size: 6247
MfeI C'AATT_G
Cuts at: 2496 3846 2496
Size: 1350 4897
MluI A'CGCG_T
Cuts at: 6123 6123
Size: 6247
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5361 5545 1305
Size: 4056 184 2007
Fragments arranged by size:
4056 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4425
Fragments arranged by size:
4425 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2042 2856 2868 2994 4205 4564 4723
Size: 648 876 814 12 126 1211 359 159
Cuts at: 4723 518
Size: 2042
Fragments arranged by size:
2042 1211 876 814 648 359 159 126
12
MspA1I CmG'CkG
Cuts at: 1606 2369 3362 3443 3513 3663 4340 5488 5733
Size: 763 993 81 70 150 677 1148 245
Cuts at: 5733 1606
Size: 2120
Fragments arranged by size:
2120 1148 993 763 677 245 150 81
70
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4756
Fragments arranged by size:
4756 939 552
NdeI CA'TA_TG
Cuts at: 387 2175 2233 387
Size: 1788 58 4401
Fragments arranged by size:
4401 1788 58
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6247
NotI GC'GGCC_GC
Cuts at: 3694 3694
Size: 6247
NsiI A_TGCA'T
Cuts at: 3315 3315
Size: 6247
NspI r_CATG'y
Cuts at: 1132 1229 2077 3605 5150 1132
Size: 97 848 1528 1545 2229
Fragments arranged by size:
2229 1545 1528 848 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6247
PciI A'CATG_T
Cuts at: 2073 5146 2073
Size: 3073 3174
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2315 1108
Size: 1207 5040
PmeI GTTT'AAAC
Cuts at: 3551 3551
Size: 6247
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4316 4348 5829 5861 4316
Size: 32 1481 32 4702
Fragments arranged by size:
4702 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2064 2210 2312 2064
Size: 146 102 5999
Fragments arranged by size:
5999 146 102
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6247
PsiI TTA'TAA
Cuts at: 3817 3817
Size: 6247
PstI C_TGCA'G
Cuts at: 830 3028 3599 830
Size: 2198 571 3478
Fragments arranged by size:
3478 2198 571
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6247
PvuII CAG'CTG
Cuts at: 2369 3362 3443 3513 2369
Size: 993 81 70 5103
Fragments arranged by size:
5103 993 81 70
SalI G'TCGA_C
Cuts at: 1936 3589 1936
Size: 1653 4594
SbfI CC_TGCA'GG
Cuts at: 3599 3599
Size: 6247
ScaI AGT'ACT
Cuts at: 2746 4495 6207 2746
Size: 1749 1712 2786
Fragments arranged by size:
2786 1749 1712
SfcI C'TryA_G
Cuts at: 826 1045 2481 2593 2920 3006 3024 3595 4730
Size: 219 1436 112 327 86 18 571 1135
Cuts at: 4730 5411 5602 826
Size: 681 191 1471
Fragments arranged by size:
1471 1436 1135 681 571 327 219 191
112 86 18
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6247
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6247
SmaI CCC'GGG
Cuts at: 1872 3574 1872
Size: 1702 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4351 5252 5514 820
Size: 197 926 2408 901 262 1553
Fragments arranged by size:
2408 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4038 493
Size: 3545 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6247
SphI G_CATG'C
Cuts at: 1132 3605 1132
Size: 2473 3774
SspI AAT'ATT
Cuts at: 5 52 3399 4171 5
Size: 47 3347 772 2081
Fragments arranged by size:
3347 2081 772 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6247
StyI C'CwwG_G
Cuts at: 513 1065 2004 2406 3686 513
Size: 552 939 402 1280 3074
Fragments arranged by size:
3074 1280 939 552 402
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2592 4274 4291 4444 4629 5074 325
Size: 1255 1012 1682 17 153 185 445 1498
Fragments arranged by size:
1682 1498 1255 1012 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2032 2744 2971
Size: 274 80 33 51 1443 55 712 227
Cuts at: 2971 4493 6205 96
Size: 1522 1712 138
Fragments arranged by size:
1712 1522 1443 712 274 227 138 80
55 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2085 2117 6207 6239 2085
Size: 32 4090 32 2093
Fragments arranged by size:
4090 2093 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6247
XbaI T'CTAG_A
Cuts at: 3583 3583
Size: 6247
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2568 2568
Size: 6247
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6247
XmaI C'CCGG_G
Cuts at: 1870 3572 1870
Size: 1702 4545
XmnI GAAnn'nnTTC
Cuts at: 4376 4376
Size: 6247
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4055 276
Size: 53 83 186 936 2521 2468
Fragments arranged by size:
2521 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI BsiWI BsmBI Bsu36I DraIII EcoICRI EcoNI
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI