Restriction map for
pFN21AB3329
(Circular) (Six-base) MAPSORT of: pFN21AB3329.seq Check: 9896 from: 1 to: 7552
pFN21AB3329 7552 bp
With 182 enzymes: SgfI *
February 10, 2010 14:44 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3646 3646
Size: 7552
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5362 278
Size: 53 83 186 936 3826 2468
Fragments arranged by size:
3826 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4154 4895 1937
Size: 2217 741 4594
Fragments arranged by size:
4594 2217 741
Acc65I G'GTAC_C
Cuts at: 4873 4873
Size: 7552
AclI AA'CG_TT
Cuts at: 1563 5679 6052 1563
Size: 4116 373 3063
Fragments arranged by size:
4116 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3066 4300 5615 6999 7267 1632
Size: 1434 1234 1315 1384 268 1917
Fragments arranged by size:
1917 1434 1384 1315 1234 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7355
AflIII A'CryG_T
Cuts at: 1203 3252 3522 3813 3902 4092 6451 7428 1203
Size: 2049 270 291 89 190 2359 977 1327
Fragments arranged by size:
2359 2049 1327 977 291 270 190 89
AgeI A'CCGG_T
Cuts at: 6383 6383
Size: 7552
AhdI GACnn_n'nnGTC
Cuts at: 6281 6281
Size: 7552
AleI CACnn'nnGTG
Cuts at: 1166 2135 4217 1166
Size: 969 2082 4501
Fragments arranged by size:
4501 2082 969
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7552
AlwNI CAG_nnn'CTG
Cuts at: 2158 2191 4073 5295 6867 2158
Size: 33 1882 1222 1572 2843
Fragments arranged by size:
2843 1882 1572 1222 33
ApaI G_GGCC'C
Cuts at: 4292 4292
Size: 7552
ApaLI G'TGCA_C
Cuts at: 3530 4460 5609 6765 3530
Size: 930 1149 1156 4317
Fragments arranged by size:
4317 1156 1149 930
ApoI r'AATT_y
Cuts at: 1493 2069 2263 4661 4861 5087 5239 7348 1493
Size: 576 194 2398 200 226 152 2109 1697
Fragments arranged by size:
2398 2109 1697 576 226 200 194 152
AseI AT'TA_AT
Cuts at: 160 1032 6106 160
Size: 872 5074 1606
Fragments arranged by size:
5074 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7552
AvaI C'yCGr_G
Cuts at: 1870 1943 3374 4877 1870
Size: 73 1431 1503 4545
Fragments arranged by size:
4545 1503 1431 73
BamHI G'GATC_C
Cuts at: 4882 5249 4882
Size: 367 7185
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2033 2129 2675 4024 4055
Size: 325 217 627 246 96 546 1349 31
Cuts at: 4055 4873 6328 618
Size: 818 1455 1842
Fragments arranged by size:
1842 1455 1349 818 627 546 325 246
217 96 31
BanII G_rGCy'C
Cuts at: 1391 4067 4292 4871 1391
Size: 2676 225 579 4072
Fragments arranged by size:
4072 2676 579 225
BbeI G_GCGC'C
Cuts at: 4028 4059 4028
Size: 31 7521
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2875 4319 928
Size: 971 976 1444 4161
Fragments arranged by size:
4161 1444 976 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5743 5777 1903
Size: 34 3806 34 3678
Fragments arranged by size:
3806 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3334 4537 5444 6654 3334
Size: 1203 907 1210 4232
Fragments arranged by size:
4232 1210 1203 907
BclI T'GATC_A
Cuts at: 1548 3114 4101 4507 1548
Size: 1566 987 406 4593
Fragments arranged by size:
4593 1566 987 406
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6163 136
Size: 107 122 71 631 605 4491 1525
Fragments arranged by size:
4491 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7547 7547
Size: 7552
BlpI GC'TnA_GC
Cuts at: 3090 4969 3090
Size: 1879 5673
Bme1580I G_kGCm'C
Cuts at: 1790 2036 3534 3910 4292 4464 5613 6769 1790
Size: 246 1498 376 382 172 1149 1156 2573
Fragments arranged by size:
2573 1498 1156 1149 382 376 246 172
BmgBI CAC'GTC
Cuts at: 1318 3343 1318
Size: 2025 5527
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3068 4317 5283
Size: 416 53 83 399 24 1651 1249 966
Cuts at: 5283 6241 442
Size: 958 1753
Fragments arranged by size:
1753 1651 1249 966 958 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7552
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3940 3940
Size: 7552
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2186 2249 2342 3605 4554 6212 1239
Size: 947 63 93 1263 949 1658 2579
Fragments arranged by size:
2579 1658 1263 949 947 93 63
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5677 6542 6840 5677
Size: 865 298 6389
Fragments arranged by size:
6389 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4757 6215 882
Size: 617 3258 1458 2219
Fragments arranged by size:
3258 2219 1458 617
BsaAI yAC'GTr
Cuts at: 493 1204 3523 3769 5343 493
Size: 711 2319 246 1574 2702
Fragments arranged by size:
2702 2319 1574 711 246
BsaBI GATnn'nnATC
Cuts at: 7546 7546
Size: 7552
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2204 3170 4025 4056
Size: 53 83 186 936 671 966 855 31
Cuts at: 4056 5359 5741 6376 275
Size: 1303 382 635 1451
Fragments arranged by size:
1451 1303 966 936 855 671 635 382
186 83 53 31
BsaWI w'CCGG_w
Cuts at: 2620 4637 5985 6383 6657 6804 7215 2620
Size: 2017 1348 398 274 147 411 2957
Fragments arranged by size:
2957 2017 1348 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3479 3509 3714 3744 4361
Size: 30 165 30 2076 30 205 30 617
Cuts at: 4361 4391 4415 4445 1178
Size: 30 24 30 4285
Fragments arranged by size:
4285 2076 617 205 165 30 30 30
30 30 30 24
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2051 2054 2129 2297 3593 4298 4364 4484
Size: 865 3 75 168 1296 705 66 120
Cuts at: 4484 4775 1186
Size: 291 3963
Fragments arranged by size:
3963 1296 865 705 291 168 120 75
66 3
BseYI C'CCAG_C
Cuts at: 3095 6755 7251 3095
Size: 3660 496 3396
Fragments arranged by size:
3660 3396 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2204 2329 1163
Size: 573 468 125 6386
Fragments arranged by size:
6386 573 468 125
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2278 3505 3752 5002 5763 6791
Size: 275 186 277 1227 247 1250 761 1028
Cuts at: 6791 1540
Size: 2301
Fragments arranged by size:
2301 1250 1227 1028 761 277 275 247
186
BsiHKAI G_wGCw'C
Cuts at: 2790 3534 4226 4280 4464 5613 5698 6769 2790
Size: 744 692 54 184 1149 85 1071 3573
Fragments arranged by size:
3573 1149 1071 744 692 184 85 54
BsmI GAATG_Cn'
Cuts at: 2503 4479 5063 5156 2503
Size: 1976 584 93 4899
Fragments arranged by size:
4899 1976 584 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2113 2805 2113
Size: 692 6860
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2036 2790 3534 3910 4067 4226 4280
Size: 399 246 754 744 376 157 159 54
Cuts at: 4280 4292 4464 4871 5613 5698 6769 1391
Size: 12 172 407 742 85 1071 2174
Fragments arranged by size:
2174 1071 754 744 742 407 399 376
246 172 159 157 85 54 12
BspEI T'CCGG_A
Cuts at: 2620 7215 2620
Size: 4595 2957
BspHI T'CATG_A
Cuts at: 5441 7171 5441
Size: 1730 5822
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2338 2984 3167 3646 4907 844
Size: 1494 646 183 479 1261 3489
Fragments arranged by size:
3489 1494 1261 646 479 183
BsrBI CCG'CTC
Cuts at: 1394 1823 2088 3490 3543 4017 5439 1394
Size: 429 265 1402 53 474 1422 3507
Fragments arranged by size:
3507 1422 1402 474 429 265 53
BsrDI GCAATG_nn'
Cuts at: 65 1248 3105 6047 6221 65
Size: 1183 1857 2942 174 1396
Fragments arranged by size:
2942 1857 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2395 3688 3838 3970 6196 6383 1700
Size: 110 585 1293 150 132 2226 187 2869
Fragments arranged by size:
2869 2226 1293 585 187 150 132 110
BsrGI T'GTAC_A
Cuts at: 96 3002 3879 96
Size: 2906 877 3769
Fragments arranged by size:
3769 2906 877
BssHII G'CGCG_C
Cuts at: 1925 3507 3609 3611 3784 4197 1925
Size: 1582 102 2 173 413 5280
Fragments arranged by size:
5280 1582 413 173 102 2
BssSI C'ACGA_G
Cuts at: 3575 5612 6624 3575
Size: 2037 1012 4503
Fragments arranged by size:
4503 2037 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3794 794
Size: 3000 4552
BstBI TT'CG_AA
Cuts at: 6356 6356
Size: 7552
BstEII G'GTnAC_C
Cuts at: 1184 3154 1184
Size: 1970 5582
BstXI CCAn_nnnn'nTGG
Cuts at: 3997 4117 4132 3997
Size: 120 15 7417
Fragments arranged by size:
7417 120 15
BstYI r'GATC_y
Cuts at: 1972 4676 4882 5249 5634 5651 7103 7189 7201
Size: 2704 206 367 385 17 1452 86 12
Cuts at: 7201 7547 1972
Size: 346 1977
Fragments arranged by size:
2704 1977 1452 385 367 346 206 86
17 12
BstZ17I GTA'TAC
Cuts at: 4155 4155
Size: 7552
BtgI C'CryG_G
Cuts at: 513 1065 3312 3327 3746 3984 513
Size: 552 2247 15 419 238 4081
Fragments arranged by size:
4081 2247 552 419 238 15
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2131 3630 4090 487
Size: 683 56 905 1499 460 3949
Fragments arranged by size:
3949 1499 905 683 460 56
BtsI GCAGTG_nn'
Cuts at: 3692 3795 4520 5068 5862 5882 3692
Size: 103 725 548 794 20 5362
Fragments arranged by size:
5362 794 725 548 103 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7552
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7517
DraI TTT'AAA
Cuts at: 2592 4609 4856 2592
Size: 2017 247 5288
Fragments arranged by size:
5288 2017 247
DraIII CAC_nnn'GTG
Cuts at: 3772 3772
Size: 7552
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2819 3064 3140 6559 809
Size: 507 1503 245 76 3419 1802
Fragments arranged by size:
3419 1802 1503 507 245 76
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2089 3315 3336
Size: 54 1425 50 275 18 259 1226 21
Cuts at: 3336 3981 3987 4517 4999 5888 7517 8
Size: 645 6 530 482 889 1629 43
Fragments arranged by size:
1629 1425 1226 889 645 530 482 275
259 54 50 43 21 18 6
EagI C'GGCC_G
Cuts at: 1537 1812 4999 1537
Size: 275 3187 4090
Fragments arranged by size:
4090 3187 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2487 4042 5482 1353
Size: 1134 1555 1440 3423
Fragments arranged by size:
3423 1555 1440 1134
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2189 6139 6513 6659 387
Size: 1064 138 600 3950 374 146 1280
Fragments arranged by size:
3950 1280 1064 600 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2186 2249 2342 3066 3605 4300 4554
Size: 393 554 63 93 724 539 695 254
Cuts at: 4554 5615 6212 6999 7267 1239
Size: 1061 597 787 268 1524
Fragments arranged by size:
1524 1061 787 724 695 597 554 539
393 268 254 93 63
EcoNI CCTnn'n_nnAGG
Cuts at: 2228 3914 4469 2228
Size: 1686 555 5311
Fragments arranged by size:
5311 1686 555
EcoO109I rG'GnC_Cy
Cuts at: 2155 2221 4288 4502 2155
Size: 66 2067 214 5205
Fragments arranged by size:
5205 2067 214 66
EcoRI G'AATT_C
Cuts at: 2069 2263 4861 5239 2069
Size: 194 2598 378 4382
Fragments arranged by size:
4382 2598 378 194
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2798 2798
Size: 7552
FspI TGC'GCA
Cuts at: 6058 6058
Size: 7552
HaeII r_GCGC'y
Cuts at: 1678 2191 3237 3977 4028 4059 4163 6699 1678
Size: 513 1046 740 51 31 104 2536 2531
Fragments arranged by size:
2536 2531 1046 740 513 104 51 31
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2034 2066 3119 3151 3785
Size: 32 135 32 462 32 1053 32 634
Cuts at: 3785 3817 4361 4393 6193 6225 6267 6299 1373
Size: 32 544 32 1800 32 42 32 2626
Fragments arranged by size:
2626 1800 1053 634 544 462 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2710 3145 3733 4090 4588 4896
Size: 988 281 772 435 588 357 498 308
Cuts at: 4896 5142 669
Size: 246 3079
Fragments arranged by size:
3079 988 772 588 498 435 357 308
281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7552
HpaI GTT'AAC
Cuts at: 3733 5142 3733
Size: 1409 6143
KasI G'GCGC_C
Cuts at: 4024 4055 4024
Size: 31 7521
KpnI G_GTAC'C
Cuts at: 4877 4877
Size: 7552
MfeI C'AATT_G
Cuts at: 5151 5151
Size: 7552
MluI A'CGCG_T
Cuts at: 3813 3902 7428 3813
Size: 89 3526 3937
Fragments arranged by size:
3937 3526 89
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3549 6666 6850 1305
Size: 2244 3117 184 2007
Fragments arranged by size:
3117 2244 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4519 10
Size: 54 1425 343 2687 3043
Fragments arranged by size:
3043 2687 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2135 4115 4217 5510 5869 6028 518
Size: 648 969 1980 102 1293 359 159 2042
Fragments arranged by size:
2042 1980 1293 969 648 359 159 102
MspA1I CmG'CkG
Cuts at: 1606 2344 3748 4599 4968 5645 6793 7038 1606
Size: 738 1404 851 369 677 1148 245 2120
Fragments arranged by size:
2120 1404 1148 851 738 677 369 245
NaeI GCC'GGC
Cuts at: 3690 3840 3972 3690
Size: 150 132 7270
Fragments arranged by size:
7270 150 132
NarI GG'CG_CC
Cuts at: 4025 4056 4025
Size: 31 7521
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 7000
NdeI CA'TA_TG
Cuts at: 387 3826 387
Size: 3439 4113
NgoMIV G'CCGG_C
Cuts at: 3688 3838 3970 3688
Size: 150 132 7270
Fragments arranged by size:
7270 150 132
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7552
NotI GC'GGCC_GC
Cuts at: 4999 4999
Size: 7552
NspI r_CATG'y
Cuts at: 1132 1229 4450 4910 6455 1132
Size: 97 3221 460 1545 2229
Fragments arranged by size:
3221 2229 1545 460 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7552
PciI A'CATG_T
Cuts at: 6451 6451
Size: 7552
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2558 2701 3772 1108
Size: 1450 143 1071 4888
Fragments arranged by size:
4888 1450 1071 143
PfoI T'CCnGG_A
Cuts at: 4040 4495 4040
Size: 455 7097
PmeI GTTT'AAAC
Cuts at: 4856 4856
Size: 7552
PmlI CAC'GTG
Cuts at: 3769 3769
Size: 7552
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5621 5653 7134 7166 5621
Size: 32 1481 32 6007
Fragments arranged by size:
6007 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2155 2221 2155
Size: 66 7486
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7552
PsiI TTA'TAA
Cuts at: 2949 5122 2949
Size: 2173 5379
PspOMI G'GGCC_C
Cuts at: 4288 4288
Size: 7552
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3371 3403 3371
Size: 32 7520
PstI C_TGCA'G
Cuts at: 830 2244 2349 3564 3657 4904 830
Size: 1414 105 1215 93 1247 3478
Fragments arranged by size:
3478 1414 1247 1215 105 93
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7552
PvuII CAG'CTG
Cuts at: 4599 4599
Size: 7552
SacII CC_GC'GG
Cuts at: 3749 3749
Size: 7552
SalI G'TCGA_C
Cuts at: 1936 4894 1936
Size: 2958 4594
SbfI CC_TGCA'GG
Cuts at: 4904 4904
Size: 7552
ScaI AGT'ACT
Cuts at: 3948 5800 7512 3948
Size: 1852 1712 3988
Fragments arranged by size:
3988 1852 1712
SexAI A'CCwGG_T
Cuts at: 4848 4848
Size: 7552
SfcI C'TryA_G
Cuts at: 826 1045 2045 2240 2345 2512 2646 2967 3560
Size: 219 1000 195 105 167 134 321 593
Cuts at: 3560 3593 3653 4900 6035 6716 6907 826
Size: 33 60 1247 1135 681 191 1471
Fragments arranged by size:
1471 1247 1135 1000 681 593 321 219
195 191 167 134 105 60 33
SfoI GGC'GCC
Cuts at: 4026 4057 4026
Size: 31 7521
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7552
SgrAI Cr'CCGG_yG
Cuts at: 1700 2395 1700
Size: 695 6857
SmaI CCC'GGG
Cuts at: 1872 4879 1872
Size: 3007 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5656 6557 6819 820
Size: 197 926 3713 901 262 1553
Fragments arranged by size:
3713 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5343 493
Size: 4850 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7552
SphI G_CATG'C
Cuts at: 1132 4450 4910 1132
Size: 3318 460 3774
Fragments arranged by size:
3774 3318 460
SspI AAT'ATT
Cuts at: 5 52 5476 5
Size: 47 5424 2081
Fragments arranged by size:
5424 2081 47
StuI AGG'CCT
Cuts at: 1517 4034 1517
Size: 2517 5035
StyI C'CwwG_G
Cuts at: 513 1065 4991 513
Size: 552 3926 3074
Fragments arranged by size:
3926 3074 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2823 3001 3746 5579 5596 5749 5934
Size: 1255 1243 178 745 1833 17 153 185
Cuts at: 5934 6379 325
Size: 445 1498
Fragments arranged by size:
1833 1498 1255 1243 745 445 185 178
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2959 3002 3378
Size: 274 80 33 51 1443 982 43 376
Cuts at: 3378 3879 3946 5798 7510 96
Size: 501 67 1852 1712 138
Fragments arranged by size:
1852 1712 1443 982 501 376 274 138
80 67 51 43 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7512 7544 7512
Size: 32 7520
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7552
XbaI T'CTAG_A
Cuts at: 4888 4888
Size: 7552
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3325 3325
Size: 7552
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7552
XmaI C'CCGG_G
Cuts at: 1870 4877 1870
Size: 3007 4545
XmnI GAAnn'nnTTC
Cuts at: 5681 5681
Size: 7552
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5360 276
Size: 53 83 186 936 3826 2468
Fragments arranged by size:
3826 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII
SacII SalI SbfI ScaI SexAI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I
BsiWI Bsu36I EcoICRI EcoRV FseI FspAI NruI NsiI
PacI PspXI RsrII SacI SanDI SapI SfiI SrfI
SwaI