Restriction map for
pFN21AB3321
(Circular) (Six-base) MAPSORT of: pFN21AB3321.seq Check: 6650 from: 1 to: 7474
pFN21AB3321 7474 bp
With 182 enzymes: SgfI *
February 27, 2009 14:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2029 2345 3790 3815 2029
Size: 316 1445 25 5688
Fragments arranged by size:
5688 1445 316 25
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5284 278
Size: 53 83 186 936 3748 2468
Fragments arranged by size:
3748 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3161 4401 4817 1937
Size: 1224 1240 416 4594
Fragments arranged by size:
4594 1240 1224 416
Acc65I G'GTAC_C
Cuts at: 4795 4795
Size: 7474
AclI AA'CG_TT
Cuts at: 1563 5601 5974 1563
Size: 4038 373 3063
Fragments arranged by size:
4038 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4739 5537 6921 7189 1632
Size: 3107 798 1384 268 1917
Fragments arranged by size:
3107 1917 1384 798 268
AfeI AGC'GCT
Cuts at: 4121 4121
Size: 7474
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7277
AflIII A'CryG_T
Cuts at: 1203 6373 7350 1203
Size: 5170 977 1327
Fragments arranged by size:
5170 1327 977
AgeI A'CCGG_T
Cuts at: 2550 3156 6305 2550
Size: 606 3149 3719
Fragments arranged by size:
3719 3149 606
AhdI GACnn_n'nnGTC
Cuts at: 2438 6203 2438
Size: 3765 3709
AleI CACnn'nnGTG
Cuts at: 1166 4499 1166
Size: 3333 4141
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3107 3867 1184
Size: 1923 760 4791
Fragments arranged by size:
4791 1923 760
AlwNI CAG_nnn'CTG
Cuts at: 2179 2215 2479 3657 3805 4090 4672 4698 4717
Size: 36 264 1178 148 285 582 26 19
Cuts at: 4717 5217 6789 2179
Size: 500 1572 2864
Fragments arranged by size:
2864 1572 1178 582 500 285 264 148
36 26 19
ApaI G_GGCC'C
Cuts at: 2565 3954 4082 4201 2565
Size: 1389 128 119 5838
Fragments arranged by size:
5838 1389 128 119
ApaLI G'TGCA_C
Cuts at: 5531 6687 5531
Size: 1156 6318
ApoI r'AATT_y
Cuts at: 1493 4783 5009 5161 7270 1493
Size: 3290 226 152 2109 1697
Fragments arranged by size:
3290 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6028 160
Size: 872 4996 1606
Fragments arranged by size:
4996 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7474
AvaI C'yCGr_G
Cuts at: 1870 1943 2558 2564 2570 2964 4194 4364 4758
Size: 73 615 6 6 394 1230 170 394
Cuts at: 4758 4799 1870
Size: 41 4545
Fragments arranged by size:
4545 1230 615 394 394 170 73 41
6 6
BamHI G'GATC_C
Cuts at: 4378 4804 5171 4378
Size: 426 367 6681
Fragments arranged by size:
6681 426 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2107 2492 3119 3176 4795
Size: 325 217 627 320 385 627 57 1619
Cuts at: 4795 6250 618
Size: 1455 1842
Fragments arranged by size:
1842 1619 1455 627 627 385 325 320
217 57
BanII G_rGCy'C
Cuts at: 1391 2095 2565 2571 2577 3642 3882 3954 3965
Size: 704 470 6 6 1065 240 72 11
Cuts at: 3965 4082 4201 4793 1391
Size: 117 119 592 4072
Fragments arranged by size:
4072 1065 704 592 470 240 119 117
72 11 6 6
BbeI G_GCGC'C
Cuts at: 3123 3123
Size: 7474
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2997 3277 928
Size: 971 1098 280 5125
Fragments arranged by size:
5125 1098 971 280
BbvCI CC'TCA_GC
Cuts at: 3257 3524 3969 4286 3257
Size: 267 445 317 6445
Fragments arranged by size:
6445 445 317 267
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2300 2334 5665 5699 1903
Size: 34 363 34 3331 34 3678
Fragments arranged by size:
3678 3331 363 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4055 5366 6576 4055
Size: 1311 1210 4953
Fragments arranged by size:
4953 1311 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7474
BfrBI ATG'CAT
Cuts at: 3584 3584
Size: 7474
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3544 3619 6085
Size: 107 122 71 631 605 1872 75 2466
Cuts at: 6085 136
Size: 1525
Fragments arranged by size:
2466 1872 1525 631 605 122 107 75
71
BglII A'GATC_T
Cuts at: 3396 7469 3396
Size: 4073 3401
BlpI GC'TnA_GC
Cuts at: 4891 4891
Size: 7474
Bme1580I G_kGCm'C
Cuts at: 1790 2497 2565 2846 3954 4082 4188 4201 5535
Size: 707 68 281 1108 128 106 13 1334
Cuts at: 5535 6691 1790
Size: 1156 2573
Fragments arranged by size:
2573 1334 1156 1108 707 281 128 106
68 13
BmgBI CAC'GTC
Cuts at: 1318 2104 1318
Size: 786 6688
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2527 4162 5205
Size: 416 53 83 399 24 1110 1635 1043
Cuts at: 5205 6163 442
Size: 958 1753
Fragments arranged by size:
1753 1635 1110 1043 958 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7474
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2296 3127 3129 2296
Size: 831 2 6641
Fragments arranged by size:
6641 831 2
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2204 2291 3122 3458 4048 4136 4787 6134
Size: 965 87 831 336 590 88 651 1347
Cuts at: 6134 1239
Size: 2579
Fragments arranged by size:
2579 1347 965 831 651 590 336 88
87
Bpu10I CC'TnA_GC
Cuts at: 2297 3257 3524 3969 4208 4286 4673 2297
Size: 960 267 445 239 78 387 5098
Fragments arranged by size:
5098 960 445 387 267 239 78
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4223 5599 6464 6762 4223
Size: 1376 865 298 4935
Fragments arranged by size:
4935 1376 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2349 6137 882
Size: 617 850 3788 2219
Fragments arranged by size:
3788 2219 850 617
BsaAI yAC'GTr
Cuts at: 493 1204 5265 493
Size: 711 4061 2702
Fragments arranged by size:
4061 2702 711
BsaBI GATnn'nnATC
Cuts at: 7468 7468
Size: 7474
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2646 3120 5281 5663
Size: 53 83 186 936 1113 474 2161 382
Cuts at: 5663 6298 275
Size: 635 1451
Fragments arranged by size:
2161 1451 1113 936 635 474 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2358 2550 3156 4344 5907 6305 6579 6726 7137
Size: 192 606 1188 1563 398 274 147 411
Cuts at: 7137 2358
Size: 2695
Fragments arranged by size:
2695 1563 1188 606 411 398 274 192
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2588 2618 1178
Size: 30 165 30 1185 30 6034
Fragments arranged by size:
6034 1185 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2258 2612 2615 2732 3579 1186
Size: 1072 354 3 117 847 5081
Fragments arranged by size:
5081 1072 847 354 117 3
BseYI C'CCAG_C
Cuts at: 2065 2303 2631 3293 3946 4075 4083 4200 6677
Size: 238 328 662 653 129 8 117 2477
Cuts at: 6677 7173 2065
Size: 496 2366
Fragments arranged by size:
2477 2366 662 653 496 328 238 129
117 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2711 3305 4544 1163
Size: 573 975 594 1239 4093
Fragments arranged by size:
4093 1239 975 594 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4924 5685 6713 1540
Size: 275 186 2923 761 1028 2301
Fragments arranged by size:
2923 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2080 2190 2298 3235 4679 5535 5620 6691 2080
Size: 110 108 937 1444 856 85 1071 2863
Fragments arranged by size:
2863 1444 1071 937 856 110 108 85
BsmI GAATG_Cn'
Cuts at: 3586 4985 5078 3586
Size: 1399 93 5982
Fragments arranged by size:
5982 1399 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3896 3896
Size: 7474
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2080 2095 2190 2298 2497 2565 2571
Size: 399 290 15 95 108 199 68 6
Cuts at: 2571 2577 2846 3235 3642 3882 3954 3965 4082
Size: 6 269 389 407 240 72 11 117
Cuts at: 4082 4188 4201 4679 4793 5535 5620 6691 1391
Size: 106 13 478 114 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 478 407 399 389 290
269 240 199 117 114 108 106 95
85 72 68 15 13 11 6 6
BspEI T'CCGG_A
Cuts at: 4344 7137 4344
Size: 2793 4681
BspHI T'CATG_A
Cuts at: 2587 3235 4480 5363 7093 2587
Size: 648 1245 883 1730 2968
Fragments arranged by size:
2968 1730 1245 883 648
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2029 2345 3528 3790 3815 4829 844
Size: 1185 316 1183 262 25 1014 3489
Fragments arranged by size:
3489 1185 1183 1014 316 262 25
BsrBI CCG'CTC
Cuts at: 1394 1823 2290 4327 5361 1394
Size: 429 467 2037 1034 3507
Fragments arranged by size:
3507 2037 1034 467 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5969 6143 65
Size: 1183 4721 174 1396
Fragments arranged by size:
4721 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2151 2550 3122 3156 3699 6118 6305
Size: 110 341 399 572 34 543 2419 187
Cuts at: 6305 1700
Size: 2869
Fragments arranged by size:
2869 2419 572 543 399 341 187 110
34
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7474
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7474
BssSI C'ACGA_G
Cuts at: 4625 5534 6546 4625
Size: 909 1012 5553
Fragments arranged by size:
5553 1012 909
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3400 3830 794
Size: 2606 430 4438
Fragments arranged by size:
4438 2606 430
BstBI TT'CG_AA
Cuts at: 2668 6278 2668
Size: 3610 3864
BstEII G'GTnAC_C
Cuts at: 1184 2884 2905 1184
Size: 1700 21 5753
Fragments arranged by size:
5753 1700 21
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 3070 3782 3874 4000 2011
Size: 1059 712 92 126 5485
Fragments arranged by size:
5485 1059 712 126 92
BstYI r'GATC_y
Cuts at: 1972 2418 2870 3189 3396 4378 4804 5171 5556
Size: 446 452 319 207 982 426 367 385
Cuts at: 5556 5573 7025 7111 7123 7469 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 982 452 446 426 385 367
346 319 207 86 17 12
BstZ17I GTA'TAC
Cuts at: 3162 4402 3162
Size: 1240 6234
Bsu36I CC'TnA_GG
Cuts at: 2372 2372
Size: 7474
BtgI C'CryG_G
Cuts at: 513 1065 2081 2577 2890 3213 3856 513
Size: 552 1016 496 313 323 643 4131
Fragments arranged by size:
4131 1016 643 552 496 323 313
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3565 487
Size: 683 56 2339 4396
Fragments arranged by size:
4396 2339 683 56
BtsI GCAGTG_nn'
Cuts at: 2393 3539 4564 4990 5784 5804 2393
Size: 1146 1025 426 794 20 4063
Fragments arranged by size:
4063 1146 1025 794 426 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7474
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7439
DraI TTT'AAA
Cuts at: 4778 4778
Size: 7474
DraIII CAC_nnn'GTG
Cuts at: 2850 3715 4054 4072 2850
Size: 865 339 18 6252
Fragments arranged by size:
6252 865 339 18
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6481 809
Size: 507 5165 1802
Fragments arranged by size:
5165 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2018 2331 2398
Size: 54 1425 50 275 18 188 313 67
Cuts at: 2398 2607 3459 3536 3701 3864 4158 4296 4539
Size: 209 852 77 165 163 294 138 243
Cuts at: 4539 4921 5810 7439 8
Size: 382 889 1629 43
Fragments arranged by size:
1629 1425 889 852 382 313 294 275
243 209 188 165 163 138 77 67
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4921 1537
Size: 275 3109 4090
Fragments arranged by size:
4090 3109 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2691 4040 5404 1353
Size: 1338 1349 1364 3423
Fragments arranged by size:
3423 1364 1349 1338
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4348 6061 6435 6581 387
Size: 1064 138 2759 1713 374 146 1280
Fragments arranged by size:
2759 1713 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2204 2291 3122 3458 4048 4136 4739
Size: 393 572 87 831 336 590 88 603
Cuts at: 4739 4787 5537 6134 6921 7189 1239
Size: 48 750 597 787 268 1524
Fragments arranged by size:
1524 831 787 750 603 597 590 572
393 336 268 88 87 48
EcoNI CCTnn'n_nnAGG
Cuts at: 2273 2918 4625 2273
Size: 645 1707 5122
Fragments arranged by size:
5122 1707 645
EcoO109I rG'GnC_Cy
Cuts at: 2639 3950 3997 4079 2639
Size: 1311 47 82 6034
Fragments arranged by size:
6034 1311 82 47
EcoRI G'AATT_C
Cuts at: 4783 5161 4783
Size: 378 7096
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3043 3043
Size: 7474
FspI TGC'GCA
Cuts at: 5980 5980
Size: 7474
HaeII r_GCGC'y
Cuts at: 1678 3123 3300 4123 6621 1678
Size: 1445 177 823 2498 2531
Fragments arranged by size:
2531 2498 1445 823 177
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2424 2456 4398 4430 6115
Size: 32 135 32 852 32 1942 32 1685
Cuts at: 6115 6147 6189 6221 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1942 1685 852 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2878 3720 3904 4818 5064 669
Size: 988 281 940 842 184 914 246 3079
Fragments arranged by size:
3079 988 940 914 842 281 246 184
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7474
HpaI GTT'AAC
Cuts at: 5064 5064
Size: 7474
KasI G'GCGC_C
Cuts at: 3119 3119
Size: 7474
KpnI G_GTAC'C
Cuts at: 4799 4799
Size: 7474
MfeI C'AATT_G
Cuts at: 5073 5073
Size: 7474
MluI A'CGCG_T
Cuts at: 7350 7350
Size: 7474
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2778 3186 3543 6588 6772 1305
Size: 1473 408 357 3045 184 2007
Fragments arranged by size:
3045 2007 1473 408 357 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2020 2333 3461 3538 3866
Size: 54 1425 343 188 313 1128 77 328
Cuts at: 3866 4160 4541 10
Size: 294 381 2943
Fragments arranged by size:
2943 1425 1128 381 343 328 313 294
188 77 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 3330 4499 5432 5791 5950 518
Size: 648 843 1321 1169 933 359 159 2042
Fragments arranged by size:
2042 1321 1169 933 843 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2083 2176 2314 2635 2941 3657 3730 3946
Size: 477 93 138 321 306 716 73 216
Cuts at: 3946 4012 4204 4717 4738 4890 5567 6715 6960
Size: 66 192 513 21 152 677 1148 245
Cuts at: 6960 1606
Size: 2120
Fragments arranged by size:
2120 1148 716 677 513 477 321 306
245 216 192 152 138 93 73 66
21
NaeI GCC'GGC
Cuts at: 3124 3701 3124
Size: 577 6897
NarI GG'CG_CC
Cuts at: 3120 3120
Size: 7474
NcoI C'CATG_G
Cuts at: 513 1065 2890 3213 513
Size: 552 1825 323 4774
Fragments arranged by size:
4774 1825 552 323
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7474
NgoMIV G'CCGG_C
Cuts at: 3122 3699 3122
Size: 577 6897
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7474
NotI GC'GGCC_GC
Cuts at: 4921 4921
Size: 7474
NsiI A_TGCA'T
Cuts at: 3586 3586
Size: 7474
NspI r_CATG'y
Cuts at: 1132 1229 4659 4832 6377 1132
Size: 97 3430 173 1545 2229
Fragments arranged by size:
3430 2229 1545 173 97
PasI CC'CwG_GG
Cuts at: 1348 2428 1348
Size: 1080 6394
PciI A'CATG_T
Cuts at: 6373 6373
Size: 7474
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2461 3421 3562 4054 4698 1108
Size: 1353 960 141 492 644 3884
Fragments arranged by size:
3884 1353 960 644 492 141
PfoI T'CCnGG_A
Cuts at: 2447 2526 2447
Size: 79 7395
PmeI GTTT'AAAC
Cuts at: 4778 4778
Size: 7474
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5543 5575 7056 7088 5543
Size: 32 1481 32 5929
Fragments arranged by size:
5929 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3997 3997
Size: 7474
PshAI GACnn'nnGTC
Cuts at: 1613 2997 1613
Size: 1384 6090
PsiI TTA'TAA
Cuts at: 5044 5044
Size: 7474
PspOMI G'GGCC_C
Cuts at: 2561 3950 4078 4197 2561
Size: 1389 128 119 5838
Fragments arranged by size:
5838 1389 128 119
PstI C_TGCA'G
Cuts at: 830 3729 3826 3846 4149 4826 830
Size: 2899 97 20 303 677 3478
Fragments arranged by size:
3478 2899 677 303 97 20
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7474
PvuII CAG'CTG
Cuts at: 2176 2314 2941 3657 3730 3946 4012 4204 4717
Size: 138 627 716 73 216 66 192 513
Cuts at: 4717 2176
Size: 4933
Fragments arranged by size:
4933 716 627 513 216 192 138 73
66
SacII CC_GC'GG
Cuts at: 2084 2084
Size: 7474
SalI G'TCGA_C
Cuts at: 1936 4816 1936
Size: 2880 4594
SapI GCTCTTCn'nnn_
Cuts at: 2691 2691
Size: 7474
SbfI CC_TGCA'GG
Cuts at: 4826 4826
Size: 7474
ScaI AGT'ACT
Cuts at: 4151 5722 7434 4151
Size: 1571 1712 4191
Fragments arranged by size:
4191 1712 1571
SexAI A'CCwGG_T
Cuts at: 3489 3981 4050 3489
Size: 492 69 6913
Fragments arranged by size:
6913 492 69
SfcI C'TryA_G
Cuts at: 826 1045 3725 3822 3842 4145 4822 5957 6638
Size: 219 2680 97 20 303 677 1135 681
Cuts at: 6638 6829 826
Size: 191 1471
Fragments arranged by size:
2680 1471 1135 681 677 303 219 191
97 20
SfoI GGC'GCC
Cuts at: 3121 3121
Size: 7474
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7474
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7474
SmaI CCC'GGG
Cuts at: 1872 2560 2966 4196 4801 1872
Size: 688 406 1230 605 4545
Fragments arranged by size:
4545 1230 688 605 406
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4238 4364 5578 6479 6741 820
Size: 197 926 2295 126 1214 901 262 1553
Fragments arranged by size:
2295 1553 1214 926 901 262 197 126
SnaBI TAC'GTA
Cuts at: 493 5265 493
Size: 4772 2702
SpeI A'CTAG_T
Cuts at: 152 4498 152
Size: 4346 3128
SphI G_CATG'C
Cuts at: 1132 4832 1132
Size: 3700 3774
SspI AAT'ATT
Cuts at: 5 52 5398 5
Size: 47 5346 2081
Fragments arranged by size:
5346 2081 47
StuI AGG'CCT
Cuts at: 1517 2279 1517
Size: 762 6712
StyI C'CwwG_G
Cuts at: 513 1065 2890 3213 3792 3993 4913 513
Size: 552 1825 323 579 201 920 3074
Fragments arranged by size:
3074 1825 920 579 552 323 201
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2547 3409 4144 5501 5518 5671 5856
Size: 1255 967 862 735 1357 17 153 185
Cuts at: 5856 6301 325
Size: 445 1498
Fragments arranged by size:
1498 1357 1255 967 862 735 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4149 4353 5720
Size: 274 80 33 51 1443 2172 204 1367
Cuts at: 5720 7432 96
Size: 1712 138
Fragments arranged by size:
2172 1712 1443 1367 274 204 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4669 4701 7434 7466 4669
Size: 32 2733 32 4677
Fragments arranged by size:
4677 2733 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7474
XbaI T'CTAG_A
Cuts at: 4810 4810
Size: 7474
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4111 4695 4111
Size: 584 6890
XhoI C'TCGA_G
Cuts at: 1943 4364 1943
Size: 2421 5053
XmaI C'CCGG_G
Cuts at: 1870 2558 2964 4194 4799 1870
Size: 688 406 1230 605 4545
Fragments arranged by size:
4545 1230 688 605 406
XmnI GAAnn'nnTTC
Cuts at: 5603 5603
Size: 7474
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5282 276
Size: 53 83 186 936 3748 2468
Fragments arranged by size:
3748 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SacII SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BsiWI EcoICRI EcoRV FseI
FspAI NruI PacI PmlI PspXI PsrI RsrII SacI
SanDI SfiI SrfI SwaI