Restriction map for
pFN21AB3319
(Circular) (Six-base) MAPSORT of: pFN21AB3319.seq Check: 5430 from: 1 to: 7123
pFN21AB3319 7123 bp
With 182 enzymes: SgfI *
October 20, 2009 10:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2193 3442 3910 2193
Size: 1249 468 5406
Fragments arranged by size:
5406 1249 468
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4933 278
Size: 53 83 186 936 3397 2468
Fragments arranged by size:
3397 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3885 4466 1937
Size: 1948 581 4594
Fragments arranged by size:
4594 1948 581
Acc65I G'GTAC_C
Cuts at: 4444 4444
Size: 7123
AclI AA'CG_TT
Cuts at: 1563 5250 5623 1563
Size: 3687 373 3063
Fragments arranged by size:
3687 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2408 3125 3749 5186 6570 6838 1632
Size: 776 717 624 1437 1384 268 1917
Fragments arranged by size:
1917 1437 1384 776 717 624 268
AfeI AGC'GCT
Cuts at: 3356 3782 4106 3356
Size: 426 324 6373
Fragments arranged by size:
6373 426 324
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6926
AflIII A'CryG_T
Cuts at: 1203 2067 2479 3616 6022 6999 1203
Size: 864 412 1137 2406 977 1327
Fragments arranged by size:
2406 1327 1137 977 864 412
AgeI A'CCGG_T
Cuts at: 5954 5954
Size: 7123
AhdI GACnn_n'nnGTC
Cuts at: 5852 5852
Size: 7123
AleI CACnn'nnGTG
Cuts at: 1166 4059 1166
Size: 2893 4230
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7123
AlwNI CAG_nnn'CTG
Cuts at: 2135 2383 3553 4325 4866 6438 2135
Size: 248 1170 772 541 1572 2820
Fragments arranged by size:
2820 1572 1170 772 541 248
ApaI G_GGCC'C
Cuts at: 2156 3267 3688 4412 2156
Size: 1111 421 724 4867
Fragments arranged by size:
4867 1111 724 421
ApaLI G'TGCA_C
Cuts at: 2180 5180 6336 2180
Size: 3000 1156 2967
Fragments arranged by size:
3000 2967 1156
ApoI r'AATT_y
Cuts at: 1493 4432 4658 4810 6919 1493
Size: 2939 226 152 2109 1697
Fragments arranged by size:
2939 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5677 160
Size: 872 4645 1606
Fragments arranged by size:
4645 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7123
AvaI C'yCGr_G
Cuts at: 1870 1943 2298 4448 1870
Size: 73 355 2150 4545
Fragments arranged by size:
4545 2150 355 73
BamHI G'GATC_C
Cuts at: 3154 4171 4453 4820 3154
Size: 1017 282 367 5457
Fragments arranged by size:
5457 1017 367 282
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2576 2747 3893 4061 4444
Size: 325 217 627 789 171 1146 168 383
Cuts at: 4444 5899 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1146 789 627 383 325 217
171 168
BanII G_rGCy'C
Cuts at: 1391 2156 2604 3267 3290 3688 3990 4412 4442
Size: 765 448 663 23 398 302 422 30
Cuts at: 4442 1391
Size: 4072
Fragments arranged by size:
4072 765 663 448 422 398 302 30
23
BbeI G_GCGC'C
Cuts at: 3897 3897
Size: 7123
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2531 928
Size: 971 632 5520
Fragments arranged by size:
5520 971 632
BbvCI CC'TCA_GC
Cuts at: 4182 4182
Size: 7123
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3033 3067 5314 5348 1903
Size: 34 1096 34 2247 34 3678
Fragments arranged by size:
3678 2247 1096 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4255 5015 6225 4255
Size: 760 1210 5153
Fragments arranged by size:
5153 1210 760
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7123
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3755 4416 5734
Size: 107 122 71 631 605 2083 661 1318
Cuts at: 5734 136
Size: 1525
Fragments arranged by size:
2083 1525 1318 661 631 605 122 107
71
BglII A'GATC_T
Cuts at: 2262 2361 7118 2262
Size: 99 4757 2267
Fragments arranged by size:
4757 2267 99
BlpI GC'TnA_GC
Cuts at: 2270 3794 4101 4108 4540 2270
Size: 1524 307 7 432 4853
Fragments arranged by size:
4853 1524 432 307 7
Bme1580I G_kGCm'C
Cuts at: 1790 2156 2184 2244 2581 2752 2967 3267 3688
Size: 366 28 60 337 171 215 300 421
Cuts at: 3688 4366 4412 5184 6340 1790
Size: 678 46 772 1156 2573
Fragments arranged by size:
2573 1156 772 678 421 366 337 300
215 171 60 46 28
BmgBI CAC'GTC
Cuts at: 1318 2068 1318
Size: 750 6373
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2551 2620 2791
Size: 416 53 83 399 24 1134 69 171
Cuts at: 2791 4854 5812 442
Size: 2063 958 1753
Fragments arranged by size:
2063 1753 1134 958 416 399 171 83
69 53 24
BmtI G_CTAG'C
Cuts at: 1056 2868 1056
Size: 1812 5311
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3826 3826
Size: 7123
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2161 2405 2576 2654 3950 4079 4177 4265
Size: 922 244 171 78 1296 129 98 88
Cuts at: 4265 4292 4358 5783 1239
Size: 27 66 1425 2579
Fragments arranged by size:
2579 1425 1296 922 244 171 129 98
88 78 66 27
Bpu10I CC'TnA_GC
Cuts at: 4182 4229 4182
Size: 47 7076
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2344 5248 6113 6411 2344
Size: 2904 865 298 3056
Fragments arranged by size:
3056 2904 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2501 3224 3797 5786 882
Size: 617 1002 723 573 1989 2219
Fragments arranged by size:
2219 1989 1002 723 617 573
BsaAI yAC'GTr
Cuts at: 493 1204 2218 2480 3617 3913 4914 493
Size: 711 1014 262 1137 296 1001 2702
Fragments arranged by size:
2702 1137 1014 1001 711 296 262
BsaBI GATnn'nnATC
Cuts at: 7117 7117
Size: 7123
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3276 3894 4930 5312
Size: 53 83 186 936 1743 618 1036 382
Cuts at: 5312 5947 275
Size: 635 1451
Fragments arranged by size:
1743 1451 1036 936 635 618 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 5556 5954 6228 6375 6786 5556
Size: 398 274 147 411 5893
Fragments arranged by size:
5893 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3125 3155 1178
Size: 30 165 30 1722 30 5146
Fragments arranged by size:
5146 1722 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2158 2183 2738 2867 3146 3230 3365 3755
Size: 972 25 555 129 279 84 135 390
Cuts at: 3755 4385 1186
Size: 630 3924
Fragments arranged by size:
3924 972 630 555 390 279 135 129
84 25
BseYI C'CCAG_C
Cuts at: 2960 3553 3676 6326 6822 2960
Size: 593 123 2650 496 3261
Fragments arranged by size:
3261 2650 593 496 123
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2372 2396 2858 3077 3320 3545 3572
Size: 573 636 24 462 219 243 225 27
Cuts at: 3572 3935 1163
Size: 363 4351
Fragments arranged by size:
4351 636 573 462 363 243 225 219
27 24
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4573 5334 6362 1540
Size: 275 186 2572 761 1028 2301
Fragments arranged by size:
2572 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2184 2604 3375 3990 4347 5184 5269 6340 2184
Size: 420 771 615 357 837 85 1071 2967
Fragments arranged by size:
2967 1071 837 771 615 420 357 85
BsmI GAATG_Cn'
Cuts at: 4634 4727 4634
Size: 93 7030
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2156 2184 2244 2581 2604 2752 2967
Size: 399 366 28 60 337 23 148 215
Cuts at: 2967 3267 3290 3375 3688 3990 4347 4366 4412
Size: 300 23 85 313 302 357 19 46
Cuts at: 4412 4442 5184 5269 6340 1391
Size: 30 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 366 357 337 313
302 300 215 148 85 85 60 46
30 28 23 23 19
BspEI T'CCGG_A
Cuts at: 6786 6786
Size: 7123
BspHI T'CATG_A
Cuts at: 5012 6742 5012
Size: 1730 5393
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2193 3099 3442 3910 4094 4478 844
Size: 1349 906 343 468 184 384 3489
Fragments arranged by size:
3489 1349 906 468 384 343 184
BsrBI CCG'CTC
Cuts at: 1394 1823 3498 5010 1394
Size: 429 1675 1512 3507
Fragments arranged by size:
3507 1675 1512 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5618 5792 65
Size: 1183 4370 174 1396
Fragments arranged by size:
4370 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3339 3636 3749 4115 5767 5954 1700
Size: 110 1529 297 113 366 1652 187 2869
Fragments arranged by size:
2869 1652 1529 366 297 187 113 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7123
BssHII G'CGCG_C
Cuts at: 1925 2049 4074 4076 1925
Size: 124 2025 2 4972
Fragments arranged by size:
4972 2025 124 2
BssSI C'ACGA_G
Cuts at: 3074 3095 4367 5183 6195 3074
Size: 21 1272 816 1012 4002
Fragments arranged by size:
4002 1272 1012 816 21
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4325 794
Size: 3531 3592
BstBI TT'CG_AA
Cuts at: 5927 5927
Size: 7123
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7123
BstXI CCAn_nnnn'nTGG
Cuts at: 2437 2929 3055 2437
Size: 492 126 6505
Fragments arranged by size:
6505 492 126
BstYI r'GATC_y
Cuts at: 1972 2262 2361 3154 3189 4171 4453 4820 5205
Size: 290 99 793 35 982 282 367 385
Cuts at: 5205 5222 6674 6760 6772 7118 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 982 793 385 367 346 290
282 99 86 35 17 12
Bsu36I CC'TnA_GG
Cuts at: 4296 4296
Size: 7123
BtgI C'CryG_G
Cuts at: 513 1065 2004 3006 3291 3873 513
Size: 552 939 1002 285 582 3763
Fragments arranged by size:
3763 1002 939 582 552 285
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6384
Fragments arranged by size:
6384 683 56
BtsI GCAGTG_nn'
Cuts at: 2588 2859 3500 3611 4639 5433 5453 2588
Size: 271 641 111 1028 794 20 4258
Fragments arranged by size:
4258 1028 794 641 271 111 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7123
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3748 3783 529
Size: 35 3184 35 3869
Fragments arranged by size:
3869 3184 35 35
DraI TTT'AAA
Cuts at: 4427 4427
Size: 7123
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6130 809
Size: 507 4814 1802
Fragments arranged by size:
4814 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2121 2947
Size: 54 1425 50 275 18 177 114 826
Cuts at: 2947 3396 3702 3756 4044 4570 5459 7088 8
Size: 449 306 54 288 526 889 1629 43
Fragments arranged by size:
1629 1425 889 826 526 449 306 288
275 177 114 54 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4570 1537
Size: 275 2758 4090
Fragments arranged by size:
4090 2758 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2880 3029 3419 5053 1353
Size: 1527 149 390 1634 3423
Fragments arranged by size:
3423 1634 1527 390 149
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2687 5710 6084 6230 387
Size: 1064 138 1098 3023 374 146 1280
Fragments arranged by size:
3023 1280 1098 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2602 3988 2602
Size: 1386 5737
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2161 2405 2408 2576 2654 3125 3749
Size: 393 529 244 3 168 78 471 624
Cuts at: 3749 3950 4079 4177 4265 4292 4358 5186 5783
Size: 201 129 98 88 27 66 828 597
Cuts at: 5783 6570 6838 1239
Size: 787 268 1524
Fragments arranged by size:
1524 828 787 624 597 529 471 393
268 244 201 168 129 98 88 78
66 27 3
EcoNI CCTnn'n_nnAGG
Cuts at: 2774 3095 4337 2774
Size: 321 1242 5560
Fragments arranged by size:
5560 1242 321
EcoO109I rG'GnC_Cy
Cuts at: 2153 2434 2914 3100 3263 3684 3685 4287 4300
Size: 281 480 186 163 421 1 602 13
Cuts at: 4300 4409 2153
Size: 109 4867
Fragments arranged by size:
4867 602 480 421 281 186 163 109
13 1
EcoRI G'AATT_C
Cuts at: 4432 4810 4432
Size: 378 6745
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2548 2650 2548
Size: 102 7021
FspI TGC'GCA
Cuts at: 4013 5629 4013
Size: 1616 5507
HaeII r_GCGC'y
Cuts at: 1678 3301 3358 3784 3897 3950 4026 4108 6270
Size: 1623 57 426 113 53 76 82 2162
Cuts at: 6270 1678
Size: 2531
Fragments arranged by size:
2531 2162 1623 426 113 82 76 57
53
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4183 4215 5764 5796 5838
Size: 32 135 32 2611 32 1549 32 42
Cuts at: 5838 5870 1373
Size: 32 2626
Fragments arranged by size:
2626 2611 1549 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4467 4713 669
Size: 988 281 2529 246 3079
Fragments arranged by size:
3079 2529 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7123
HpaI GTT'AAC
Cuts at: 4713 4713
Size: 7123
KasI G'GCGC_C
Cuts at: 3893 3893
Size: 7123
KpnI G_GTAC'C
Cuts at: 4448 4448
Size: 7123
MfeI C'AATT_G
Cuts at: 4722 4722
Size: 7123
MluI A'CGCG_T
Cuts at: 6999 6999
Size: 7123
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3925 6237 6421 1305
Size: 2620 2312 184 2007
Fragments arranged by size:
2620 2312 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 2123 3398 3758 10
Size: 54 1425 343 177 114 1275 360 3375
Fragments arranged by size:
3375 1425 1275 360 343 177 114 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2544 3854 4059 5081 5440 5599 518
Size: 648 1378 1310 205 1022 359 159 2042
Fragments arranged by size:
2042 1378 1310 1022 648 359 205 159
MspA1I CmG'CkG
Cuts at: 1606 2206 2313 2403 3334 3553 3798 4139 4207
Size: 600 107 90 931 219 245 341 68
Cuts at: 4207 4539 5216 6364 6609 1606
Size: 332 677 1148 245 2120
Fragments arranged by size:
2120 1148 931 677 600 341 332 245
245 219 107 90 68
NaeI GCC'GGC
Cuts at: 3751 4117 3751
Size: 366 6757
NarI GG'CG_CC
Cuts at: 3894 3894
Size: 7123
NcoI C'CATG_G
Cuts at: 513 1065 2004 3006 513
Size: 552 939 1002 4630
Fragments arranged by size:
4630 1002 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7123
NgoMIV G'CCGG_C
Cuts at: 3749 4115 3749
Size: 366 6757
NheI G'CTAG_C
Cuts at: 1052 2864 1052
Size: 1812 5311
NotI GC'GGCC_GC
Cuts at: 4570 4570
Size: 7123
NspI r_CATG'y
Cuts at: 1132 1229 3085 4481 6026 1132
Size: 97 1856 1396 1545 2229
Fragments arranged by size:
2229 1856 1545 1396 97
PasI CC'CwG_GG
Cuts at: 1348 4147 4405 1348
Size: 2799 258 4066
Fragments arranged by size:
4066 2799 258
PciI A'CATG_T
Cuts at: 6022 6022
Size: 7123
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2206 2503 3553 1108
Size: 1098 297 1050 4678
Fragments arranged by size:
4678 1098 1050 297
PfoI T'CCnGG_A
Cuts at: 3630 3630
Size: 7123
PmeI GTTT'AAAC
Cuts at: 4427 4427
Size: 7123
PmlI CAC'GTG
Cuts at: 2480 3617 3913 2480
Size: 1137 296 5690
Fragments arranged by size:
5690 1137 296
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5192 5224 6705 6737 5192
Size: 32 1481 32 5578
Fragments arranged by size:
5578 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2434 2914 3100 4300 2434
Size: 480 186 1200 5257
Fragments arranged by size:
5257 1200 480 186
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7123
PsiI TTA'TAA
Cuts at: 4693 4693
Size: 7123
PspOMI G'GGCC_C
Cuts at: 2152 3263 3684 4408 2152
Size: 1111 421 724 4867
Fragments arranged by size:
4867 1111 724 421
PstI C_TGCA'G
Cuts at: 830 3606 4124 4475 830
Size: 2776 518 351 3478
Fragments arranged by size:
3478 2776 518 351
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7123
PvuII CAG'CTG
Cuts at: 2206 2313 3334 3553 4139 4207 2206
Size: 107 1021 219 586 68 5122
Fragments arranged by size:
5122 1021 586 219 107 68
SacI G_AGCT'C
Cuts at: 2604 3990 2604
Size: 1386 5737
SalI G'TCGA_C
Cuts at: 1936 4465 1936
Size: 2529 4594
SapI GCTCTTCn'nnn_
Cuts at: 2880 2880
Size: 7123
SbfI CC_TGCA'GG
Cuts at: 4475 4475
Size: 7123
ScaI AGT'ACT
Cuts at: 5371 7083 5371
Size: 1712 5411
SfcI C'TryA_G
Cuts at: 826 1045 3602 4120 4471 5606 6287 6478 826
Size: 219 2557 518 351 1135 681 191 1471
Fragments arranged by size:
2557 1471 1135 681 518 351 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 4416 4416
Size: 7123
SfoI GGC'GCC
Cuts at: 3895 3895
Size: 7123
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7123
SgrAI Cr'CCGG_yG
Cuts at: 1700 3339 1700
Size: 1639 5484
SmaI CCC'GGG
Cuts at: 1872 4450 1872
Size: 2578 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2323 5227 6128 6390 820
Size: 197 926 380 2904 901 262 1553
Fragments arranged by size:
2904 1553 926 901 380 262 197
SnaBI TAC'GTA
Cuts at: 493 4914 493
Size: 4421 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7123
SphI G_CATG'C
Cuts at: 1132 3085 4481 1132
Size: 1953 1396 3774
Fragments arranged by size:
3774 1953 1396
SspI AAT'ATT
Cuts at: 5 52 5047 5
Size: 47 4995 2081
Fragments arranged by size:
4995 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7123
StyI C'CwwG_G
Cuts at: 513 1065 2004 2061 2871 2895 3006 3516 3559
Size: 552 939 57 810 24 111 510 43
Cuts at: 3559 4414 4562 513
Size: 855 148 3074
Fragments arranged by size:
3074 939 855 810 552 510 148 111
57 43 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2735 5150 5167 5320 5505 5950 325
Size: 1255 1155 2415 17 153 185 445 1498
Fragments arranged by size:
2415 1498 1255 1155 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2172 3141 5369
Size: 274 80 33 51 1443 195 969 2228
Cuts at: 5369 7081 96
Size: 1712 138
Fragments arranged by size:
2228 1712 1443 969 274 195 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2731 2763 3423 3455 7083 7115 2731
Size: 32 660 32 3628 32 2739
Fragments arranged by size:
3628 2739 660 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2234 2783 1316
Size: 918 549 5656
Fragments arranged by size:
5656 918 549
XbaI T'CTAG_A
Cuts at: 4459 4459
Size: 7123
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2688 2688
Size: 7123
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7123
XmaI C'CCGG_G
Cuts at: 1870 4448 1870
Size: 2578 4545
XmnI GAAnn'nnTTC
Cuts at: 2633 5252 2633
Size: 2619 4504
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4931 276
Size: 53 83 186 936 3397 2468
Fragments arranged by size:
3397 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SalI SapI SbfI ScaI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I
DraIII EcoRV FseI FspAI NruI NsiI PacI PspXI
PsrI RsrII SacII SanDI SexAI SrfI SwaI