Restriction map for
pFN21AB3314
(Circular) (Six-base) MAPSORT of: pFN21AB3314.seq Check: 7291 from: 1 to: 6832
pFN21AB3314 6832 bp
With 182 enzymes: SgfI *
October 20, 2009 10:36 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2523 3710 4642 278
Size: 53 83 186 936 987 1187 932 2468
Fragments arranged by size:
2468 1187 987 936 932 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4175 1937
Size: 2238 4594
Acc65I G'GTAC_C
Cuts at: 3577 4153 3577
Size: 576 6256
AclI AA'CG_TT
Cuts at: 1563 4959 5332 1563
Size: 3396 373 3063
Fragments arranged by size:
3396 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2157 3184 3693 4011 4895 6279 6547 1632
Size: 525 1027 509 318 884 1384 268 1917
Fragments arranged by size:
1917 1384 1027 884 525 509 318 268
AflII C'TTAA_G
Cuts at: 820 1017 2186 820
Size: 197 1169 5466
Fragments arranged by size:
5466 1169 197
AflIII A'CryG_T
Cuts at: 1203 2585 3944 5731 6708 1203
Size: 1382 1359 1787 977 1327
Fragments arranged by size:
1787 1382 1359 1327 977
AgeI A'CCGG_T
Cuts at: 5663 5663
Size: 6832
AhdI GACnn_n'nnGTC
Cuts at: 2284 2509 3259 5561 2284
Size: 225 750 2302 3555
Fragments arranged by size:
3555 2302 750 225
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6832
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6832
AlwNI CAG_nnn'CTG
Cuts at: 2294 4575 6147 2294
Size: 2281 1572 2979
Fragments arranged by size:
2979 2281 1572
ApaLI G'TGCA_C
Cuts at: 3545 4889 6045 3545
Size: 1344 1156 4332
Fragments arranged by size:
4332 1344 1156
ApoI r'AATT_y
Cuts at: 1493 2033 2194 2561 3775 4141 4367 4519 6628
Size: 540 161 367 1214 366 226 152 2109
Cuts at: 6628 1493
Size: 1697
Fragments arranged by size:
2109 1697 1214 540 367 366 226 161
152
AseI AT'TA_AT
Cuts at: 160 1032 3362 5386 160
Size: 872 2330 2024 1606
Fragments arranged by size:
2330 2024 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6832
AvaI C'yCGr_G
Cuts at: 1870 1943 4157 1870
Size: 73 2214 4545
Fragments arranged by size:
4545 2214 73
BamHI G'GATC_C
Cuts at: 4162 4529 4162
Size: 367 6465
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3577 4153 5608 618
Size: 325 217 627 1790 576 1455 1842
Fragments arranged by size:
1842 1790 1455 627 576 325 217
BanII G_rGCy'C
Cuts at: 1391 2274 4151 1391
Size: 883 1877 4072
Fragments arranged by size:
4072 1877 883
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3068 928
Size: 971 1169 4692
Fragments arranged by size:
4692 1169 971
BbvCI CC'TCA_GC
Cuts at: 2105 2105
Size: 6832
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3536 3570 5023 5057 1903
Size: 34 1599 34 1453 34 3678
Fragments arranged by size:
3678 1599 1453 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2251 2654 4724 5934 2251
Size: 403 2070 1210 3149
Fragments arranged by size:
3149 2070 1210 403
BclI T'GATC_A
Cuts at: 1548 2598 1548
Size: 1050 5782
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5443 136
Size: 107 122 71 631 605 3771 1525
Fragments arranged by size:
3771 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2182 2775 2885 6827 2182
Size: 593 110 3942 2187
Fragments arranged by size:
3942 2187 593 110
BlpI GC'TnA_GC
Cuts at: 4249 4249
Size: 6832
Bme1580I G_kGCm'C
Cuts at: 1790 3481 3549 4893 6049 1790
Size: 1691 68 1344 1156 2573
Fragments arranged by size:
2573 1691 1344 1156 68
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6832
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4563 5521 442
Size: 416 53 83 399 24 3146 958 1753
Fragments arranged by size:
3146 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6832
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2062 2064 2062
Size: 2 6830
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2792 2915 3001 3209 3732 5492 1239
Size: 1553 123 86 208 523 1760 2579
Fragments arranged by size:
2579 1760 1553 523 208 123 86
Bpu10I CC'TnA_GC
Cuts at: 2105 2357 2105
Size: 252 6580
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4957 5822 6120 4957
Size: 865 298 5669
Fragments arranged by size:
5669 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5495 882
Size: 617 3996 2219
Fragments arranged by size:
3996 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3945 4623 493
Size: 711 2741 678 2702
Fragments arranged by size:
2741 2702 711 678
BsaBI GATnn'nnATC
Cuts at: 4050 6826 4050
Size: 2776 4056
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2520 3707 4639 5021
Size: 53 83 186 936 987 1187 932 382
Cuts at: 5021 5656 275
Size: 635 1451
Fragments arranged by size:
1451 1187 987 936 932 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2065 2822 5265 5663 5937 6084 6495 2065
Size: 757 2443 398 274 147 411 2402
Fragments arranged by size:
2443 2402 757 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2417 2447 1178
Size: 30 165 30 1014 30 5563
Fragments arranged by size:
5563 1014 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2377 2723 3206 3234 3602 3650 1186
Size: 1191 346 483 28 368 48 4368
Fragments arranged by size:
4368 1191 483 368 346 48 28
BseYI C'CCAG_C
Cuts at: 2411 3226 6035 6531 2411
Size: 815 2809 496 2712
Fragments arranged by size:
2809 2712 815 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2426 2610 2934 1163
Size: 573 690 184 324 5061
Fragments arranged by size:
5061 690 573 324 184
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4282 5043 6071 1540
Size: 275 186 2281 761 1028 2301
Fragments arranged by size:
2301 2281 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2274 3549 4893 4978 6049 2274
Size: 1275 1344 85 1071 3057
Fragments arranged by size:
3057 1344 1275 1071 85
BsmI GAATG_Cn'
Cuts at: 4343 4436 4343
Size: 93 6739
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2274 3481 3549 4151 4893 4978 6049
Size: 399 484 1207 68 602 742 85 1071
Cuts at: 6049 1391
Size: 2174
Fragments arranged by size:
2174 1207 1071 742 602 484 399 85
68
BspEI T'CCGG_A
Cuts at: 6495 6495
Size: 6832
BspHI T'CATG_A
Cuts at: 4721 6451 4721
Size: 1730 5102
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2067 4187 844
Size: 1223 2120 3489
Fragments arranged by size:
3489 2120 1223
BsrBI CCG'CTC
Cuts at: 1394 1823 4719 1394
Size: 429 2896 3507
Fragments arranged by size:
3507 2896 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3063 5327 5501 65
Size: 1183 1815 2264 174 1396
Fragments arranged by size:
2264 1815 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5476 5663 1700
Size: 110 3666 187 2869
Fragments arranged by size:
3666 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6832
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6832
BssSI C'ACGA_G
Cuts at: 3765 4892 5904 3765
Size: 1127 1012 4693
Fragments arranged by size:
4693 1127 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6832
BstBI TT'CG_AA
Cuts at: 2992 5636 2992
Size: 2644 4188
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6832
BstYI r'GATC_y
Cuts at: 1972 2182 2613 2775 2885 4162 4529 4914 4931
Size: 210 431 162 110 1277 367 385 17
Cuts at: 4931 6383 6469 6481 6827 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1277 431 385 367 346 210
162 110 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2098 513
Size: 552 939 94 5247
Fragments arranged by size:
5247 939 552 94
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3152 487
Size: 683 56 1926 4167
Fragments arranged by size:
4167 1926 683 56
BtsI GCAGTG_nn'
Cuts at: 2409 4348 5142 5162 2409
Size: 1939 794 20 4079
Fragments arranged by size:
4079 1939 794 20
ClaI AT'CG_AT
Cuts at: 1554 3366 1554
Size: 1812 5020
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2200 2235 3253 3288 3748 3783 529
Size: 35 1636 35 1018 35 460 35 3578
Fragments arranged by size:
3578 1636 1018 460 35 35 35 35
DraI TTT'AAA
Cuts at: 3523 3991 4136 3523
Size: 468 145 6219
Fragments arranged by size:
6219 468 145
DraIII CAC_nnn'GTG
Cuts at: 2404 2404
Size: 6832
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5839 809
Size: 507 4523 1802
Fragments arranged by size:
4523 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3018 3627 4279
Size: 54 1425 50 275 18 1188 609 652
Cuts at: 4279 5168 6797 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1188 889 652 609 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4279 1537
Size: 275 2467 4090
Fragments arranged by size:
4090 2467 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4762 1353
Size: 3409 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5419 5793 5939 387
Size: 1064 138 3830 374 146 1280
Fragments arranged by size:
3830 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2272 2272
Size: 6832
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2157 2792 2915 3001 3184 3209 3693
Size: 393 525 635 123 86 183 25 484
Cuts at: 3693 3732 4011 4895 5492 6279 6547 1239
Size: 39 279 884 597 787 268 1524
Fragments arranged by size:
1524 884 787 635 597 525 484 393
279 268 183 123 86 39 25
EcoNI CCTnn'n_nnAGG
Cuts at: 2534 2969 2534
Size: 435 6397
EcoO109I rG'GnC_Cy
Cuts at: 2647 3157 2647
Size: 510 6322
EcoRI G'AATT_C
Cuts at: 4141 4519 4141
Size: 378 6454
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2530 2934 2530
Size: 404 6428
FspI TGC'GCA
Cuts at: 5338 5338
Size: 6832
HaeII r_GCGC'y
Cuts at: 1678 5979 1678
Size: 4301 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2270 2302 2495 2527 3245
Size: 32 135 32 698 32 193 32 718
Cuts at: 3245 3277 3340 3359 3372 3391 3435 3467 3779
Size: 32 63 19 13 19 44 32 312
Cuts at: 3779 3811 4060 4092 5473 5505 5547 5579 1373
Size: 32 249 32 1381 32 42 32 2626
Fragments arranged by size:
2626 1381 718 698 312 249 193 135
63 44 42 32 32 32 32 32
32 32 32 32 32 19 19 13
HincII GTy'rAC
Cuts at: 669 1657 1938 3508 4176 4422 669
Size: 988 281 1570 668 246 3079
Fragments arranged by size:
3079 1570 988 668 281 246
HindIII A'AGCT_T
Cuts at: 748 2169 748
Size: 1421 5411
HpaI GTT'AAC
Cuts at: 4422 4422
Size: 6832
KpnI G_GTAC'C
Cuts at: 3581 4157 3581
Size: 576 6256
MfeI C'AATT_G
Cuts at: 4431 4431
Size: 6832
MluI A'CGCG_T
Cuts at: 6708 6708
Size: 6832
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2437 3067 5946 6130 1305
Size: 1132 630 2879 184 2007
Fragments arranged by size:
2879 2007 1132 630 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3020 10
Size: 54 1425 343 1188 3822
Fragments arranged by size:
3822 1425 1188 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2910 3026 4790 5149 5308 518
Size: 648 1744 116 1764 359 159 2042
Fragments arranged by size:
2042 1764 1744 648 359 159 116
MspA1I CmG'CkG
Cuts at: 1606 2100 2477 2798 4248 4925 6073 6318 1606
Size: 494 377 321 1450 677 1148 245 2120
Fragments arranged by size:
2120 1450 1148 677 494 377 321 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5341
Fragments arranged by size:
5341 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6832
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6832
NotI GC'GGCC_GC
Cuts at: 4279 4279
Size: 6832
NspI r_CATG'y
Cuts at: 1132 1229 4190 5735 1132
Size: 97 2961 1545 2229
Fragments arranged by size:
2961 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6832
PciI A'CATG_T
Cuts at: 5731 5731
Size: 6832
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6832
PfoI T'CCnGG_A
Cuts at: 2253 3186 2253
Size: 933 5899
PmeI GTTT'AAAC
Cuts at: 4136 4136
Size: 6832
PmlI CAC'GTG
Cuts at: 3945 3945
Size: 6832
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4901 4933 6414 6446 4901
Size: 32 1481 32 5287
Fragments arranged by size:
5287 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3157 3157
Size: 6832
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6832
PsiI TTA'TAA
Cuts at: 4402 4402
Size: 6832
PstI C_TGCA'G
Cuts at: 830 3075 4184 830
Size: 2245 1109 3478
Fragments arranged by size:
3478 2245 1109
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6832
PvuII CAG'CTG
Cuts at: 2477 2798 2477
Size: 321 6511
SacI G_AGCT'C
Cuts at: 2274 2274
Size: 6832
SacII CC_GC'GG
Cuts at: 2101 2101
Size: 6832
SalI G'TCGA_C
Cuts at: 1936 4174 1936
Size: 2238 4594
SbfI CC_TGCA'GG
Cuts at: 4184 4184
Size: 6832
ScaI AGT'ACT
Cuts at: 5080 6792 5080
Size: 1712 5120
SfcI C'TryA_G
Cuts at: 826 1045 3071 4180 5315 5996 6187 826
Size: 219 2026 1109 1135 681 191 1471
Fragments arranged by size:
2026 1471 1135 1109 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6832
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6832
SmaI CCC'GGG
Cuts at: 1872 4159 1872
Size: 2287 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2186 4936 5837 6099 820
Size: 197 926 243 2750 901 262 1553
Fragments arranged by size:
2750 1553 926 901 262 243 197
SnaBI TAC'GTA
Cuts at: 493 4623 493
Size: 4130 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6832
SphI G_CATG'C
Cuts at: 1132 4190 1132
Size: 3058 3774
SspI AAT'ATT
Cuts at: 5 52 2953 4756 5
Size: 47 2901 1803 2081
Fragments arranged by size:
2901 2081 1803 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6832
StyI C'CwwG_G
Cuts at: 513 1065 2004 2019 2997 3573 4271 513
Size: 552 939 15 978 576 698 3074
Fragments arranged by size:
3074 978 939 698 576 552 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2844 3270 3356 4859 4876 5029 5214
Size: 1255 1264 426 86 1503 17 153 185
Cuts at: 5214 5659 325
Size: 445 1498
Fragments arranged by size:
1503 1498 1264 1255 445 426 185 153
86 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2890 5078 6790
Size: 274 80 33 51 1443 913 2188 1712
Cuts at: 6790 96
Size: 138
Fragments arranged by size:
2188 1712 1443 913 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6792 6824 6792
Size: 32 6800
Tth111I GACn'n_nGTC
Cuts at: 1316 2854 3178 1316
Size: 1538 324 4970
Fragments arranged by size:
4970 1538 324
XbaI T'CTAG_A
Cuts at: 4168 4168
Size: 6832
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3150 3150
Size: 6832
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6832
XmaI C'CCGG_G
Cuts at: 1870 4157 1870
Size: 2287 4545
XmnI GAAnn'nnTTC
Cuts at: 3877 4961 3877
Size: 1084 5748
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2521 3708 4640 276
Size: 53 83 186 936 987 1187 932 2468
Fragments arranged by size:
2468 1187 987 936 932 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI
SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PspOMI PspXI PsrI RsrII SanDI SapI SexAI SfiI
SfoI SrfI SwaI