Restriction map for
pFN21AB3312
(Circular) (Six-base) MAPSORT of: pFN21AB3312.seq Check: 6199 from: 1 to: 7177
pFN21AB3312 7177 bp
With 182 enzymes: SgfI *
October 20, 2009 10:33 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4987 278
Size: 53 83 186 936 3451 2468
Fragments arranged by size:
3451 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4520 1937
Size: 2583 4594
Acc65I G'GTAC_C
Cuts at: 4498 4498
Size: 7177
AclI AA'CG_TT
Cuts at: 1563 5304 5677 1563
Size: 3741 373 3063
Fragments arranged by size:
3741 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3867 5240 6624 6892 1632
Size: 2235 1373 1384 268 1917
Fragments arranged by size:
2235 1917 1384 1373 268
AfeI AGC'GCT
Cuts at: 3987 3987
Size: 7177
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6980
AflIII A'CryG_T
Cuts at: 1203 2371 6076 7053 1203
Size: 1168 3705 977 1327
Fragments arranged by size:
3705 1327 1168 977
AgeI A'CCGG_T
Cuts at: 6008 6008
Size: 7177
AhdI GACnn_n'nnGTC
Cuts at: 5906 5906
Size: 7177
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7177
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2240 2558 1184
Size: 1056 318 5803
Fragments arranged by size:
5803 1056 318
AlwNI CAG_nnn'CTG
Cuts at: 2495 4920 6492 2495
Size: 2425 1572 3180
Fragments arranged by size:
3180 2425 1572
ApaLI G'TGCA_C
Cuts at: 5234 6390 5234
Size: 1156 6021
ApoI r'AATT_y
Cuts at: 1493 2510 2650 3697 3767 3953 4121 4279 4472
Size: 1017 140 1047 70 186 168 158 193
Cuts at: 4472 4486 4712 4864 6973 1493
Size: 14 226 152 2109 1697
Fragments arranged by size:
2109 1697 1047 1017 226 193 186 168
158 152 140 70 14
AseI AT'TA_AT
Cuts at: 160 1032 5731 160
Size: 872 4699 1606
Fragments arranged by size:
4699 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7177
AvaI C'yCGr_G
Cuts at: 1870 1943 4502 1870
Size: 73 2559 4545
Fragments arranged by size:
4545 2559 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2550 2583 2550
Size: 33 7144
BamHI G'GATC_C
Cuts at: 2888 4507 4874 2888
Size: 1619 367 5191
Fragments arranged by size:
5191 1619 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4498 5953 618
Size: 325 217 627 2711 1455 1842
Fragments arranged by size:
2711 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2950 3098 4496 1391
Size: 1559 148 1398 4072
Fragments arranged by size:
4072 1559 1398 148
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2992 928
Size: 971 1093 5113
Fragments arranged by size:
5113 1093 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5368 5402 1903
Size: 34 3431 34 3678
Fragments arranged by size:
3678 3431 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4066 5069 6279 4066
Size: 1003 1210 4964
Fragments arranged by size:
4964 1210 1003
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7177
BfrBI ATG'CAT
Cuts at: 3914 3973 3914
Size: 59 7118
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5788 136
Size: 107 122 71 631 605 4116 1525
Fragments arranged by size:
4116 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3615 7172 3615
Size: 3557 3620
BlpI GC'TnA_GC
Cuts at: 4594 4594
Size: 7177
Bme1580I G_kGCm'C
Cuts at: 1790 5238 6394 1790
Size: 3448 1156 2573
Fragments arranged by size:
3448 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7177
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2753 4908 5866
Size: 416 53 83 399 24 1336 2155 958
Cuts at: 5866 442
Size: 1753
Fragments arranged by size:
2155 1753 1336 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7177
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3637 3637
Size: 7177
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2901 5837 1239
Size: 1662 2936 2579
Fragments arranged by size:
2936 2579 1662
Bpu10I CC'TnA_GC
Cuts at: 2155 2155
Size: 7177
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5302 6167 6465 5302
Size: 865 298 6014
Fragments arranged by size:
6014 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2457 5840 882
Size: 617 958 3383 2219
Fragments arranged by size:
3383 2219 958 617
BsaAI yAC'GTr
Cuts at: 493 1204 4968 493
Size: 711 3764 2702
Fragments arranged by size:
3764 2702 711
BsaBI GATnn'nnATC
Cuts at: 4302 7171 4302
Size: 2869 4308
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4984 5366 6001 275
Size: 53 83 186 936 3451 382 635 1451
Fragments arranged by size:
3451 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2942 5610 6008 6282 6429 6840 2942
Size: 2668 398 274 147 411 3279
Fragments arranged by size:
3279 2668 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6952
Fragments arranged by size:
6952 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 4382 1186
Size: 3196 3981
BseYI C'CCAG_C
Cuts at: 6380 6876 6380
Size: 496 6681
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6604
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4627 5388 6416 1540
Size: 275 186 2626 761 1028 2301
Fragments arranged by size:
2626 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 5238 5323 6394 5238
Size: 85 1071 6021
Fragments arranged by size:
6021 1071 85
BsmI GAATG_Cn'
Cuts at: 4688 4781 4688
Size: 93 7084
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2950 3098 4496 5238 5323 6394 1391
Size: 399 1160 148 1398 742 85 1071 2174
Fragments arranged by size:
2174 1398 1160 1071 742 399 148 85
BspEI T'CCGG_A
Cuts at: 6840 6840
Size: 7177
BspHI T'CATG_A
Cuts at: 5066 6796 5066
Size: 1730 5447
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2232 4532 844
Size: 1388 2300 3489
Fragments arranged by size:
3489 2300 1388
BsrBI CCG'CTC
Cuts at: 1394 1823 2154 5064 1394
Size: 429 331 2910 3507
Fragments arranged by size:
3507 2910 429 331
BsrDI GCAATG_nn'
Cuts at: 65 1248 2219 5672 5846 65
Size: 1183 971 3453 174 1396
Fragments arranged by size:
3453 1396 1183 971 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5821 6008 1700
Size: 110 4011 187 2869
Fragments arranged by size:
4011 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7177
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7177
BssSI C'ACGA_G
Cuts at: 5237 6249 5237
Size: 1012 6165
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7177
BstBI TT'CG_AA
Cuts at: 3877 5981 3877
Size: 2104 5073
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7177
BstXI CCAn_nnnn'nTGG
Cuts at: 3711 3711
Size: 7177
BstYI r'GATC_y
Cuts at: 1972 2491 2888 3615 4303 4507 4874 5259 5276
Size: 519 397 727 688 204 367 385 17
Cuts at: 5276 6728 6814 6826 7172 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 727 688 519 397 385 367
346 204 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3084 3084
Size: 7177
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6625
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6438
Fragments arranged by size:
6438 683 56
BtsI GCAGTG_nn'
Cuts at: 3122 4693 5487 5507 3122
Size: 1571 794 20 4792
Fragments arranged by size:
4792 1571 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7177
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7142
DraI TTT'AAA
Cuts at: 2436 2509 4481 2436
Size: 73 1972 5132
Fragments arranged by size:
5132 1972 73
DraIII CAC_nnn'GTG
Cuts at: 3011 3011
Size: 7177
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6184 809
Size: 507 4868 1802
Fragments arranged by size:
4868 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2977 4624 5513
Size: 54 1425 50 275 18 1147 1647 889
Cuts at: 5513 7142 8
Size: 1629 43
Fragments arranged by size:
1647 1629 1425 1147 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4624 1537
Size: 275 2812 4090
Fragments arranged by size:
4090 2812 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3310 5107 1353
Size: 1957 1797 3423
Fragments arranged by size:
3423 1957 1797
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5764 6138 6284 387
Size: 1064 138 4175 374 146 1280
Fragments arranged by size:
4175 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2901 3867 5240 5837 6624 6892 1239
Size: 393 1269 966 1373 597 787 268 1524
Fragments arranged by size:
1524 1373 1269 966 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 3525 3525
Size: 7177
EcoRI G'AATT_C
Cuts at: 4486 4864 4486
Size: 378 6799
EcoRV GAT'ATC
Cuts at: 3994 3994
Size: 7177
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2695 3016 2695
Size: 321 6856
FspI TGC'GCA
Cuts at: 5683 5683
Size: 7177
HaeII r_GCGC'y
Cuts at: 1678 3989 6324 1678
Size: 2311 2335 2531
Fragments arranged by size:
2531 2335 2311
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2921 2953 5818 5850 5892
Size: 32 135 32 1349 32 2865 32 42
Cuts at: 5892 5924 1373
Size: 32 2626
Fragments arranged by size:
2865 2626 1349 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4521 4767 669
Size: 988 281 2583 246 3079
Fragments arranged by size:
3079 2583 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2255 748
Size: 1507 5670
HpaI GTT'AAC
Cuts at: 4767 4767
Size: 7177
KpnI G_GTAC'C
Cuts at: 4502 4502
Size: 7177
MfeI C'AATT_G
Cuts at: 2631 4338 4776 2631
Size: 1707 438 5032
Fragments arranged by size:
5032 1707 438
MluI A'CGCG_T
Cuts at: 7053 7053
Size: 7177
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6291 6475 1305
Size: 4986 184 2007
Fragments arranged by size:
4986 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5355
Fragments arranged by size:
5355 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2212 3909 5135 5494 5653 518
Size: 648 843 203 1697 1226 359 159 2042
Fragments arranged by size:
2042 1697 1226 843 648 359 203 159
MspA1I CmG'CkG
Cuts at: 1606 2717 4460 4593 5270 6418 6663 1606
Size: 1111 1743 133 677 1148 245 2120
Fragments arranged by size:
2120 1743 1148 1111 677 245 133
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6625
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7177
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7177
NotI GC'GGCC_GC
Cuts at: 4624 4624
Size: 7177
NsiI A_TGCA'T
Cuts at: 3916 3975 3916
Size: 59 7118
NspI r_CATG'y
Cuts at: 1132 1229 2375 3553 4046 4535 6080 1132
Size: 97 1146 1178 493 489 1545 2229
Fragments arranged by size:
2229 1545 1178 1146 493 489 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7177
PciI A'CATG_T
Cuts at: 2371 6076 2371
Size: 3705 3472
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2795 2849 2879 1108
Size: 1687 54 30 5406
Fragments arranged by size:
5406 1687 54 30
PfoI T'CCnGG_A
Cuts at: 2878 2878
Size: 7177
PmeI GTTT'AAAC
Cuts at: 4481 4481
Size: 7177
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5246 5278 6759 6791 5246
Size: 32 1481 32 5632
Fragments arranged by size:
5632 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7177
PsiI TTA'TAA
Cuts at: 3909 4747 3909
Size: 838 6339
PstI C_TGCA'G
Cuts at: 830 2019 3724 4529 830
Size: 1189 1705 805 3478
Fragments arranged by size:
3478 1705 1189 805
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7177
PvuII CAG'CTG
Cuts at: 2717 4460 2717
Size: 1743 5434
SalI G'TCGA_C
Cuts at: 1936 4519 1936
Size: 2583 4594
SbfI CC_TGCA'GG
Cuts at: 4529 4529
Size: 7177
ScaI AGT'ACT
Cuts at: 2675 5425 7137 2675
Size: 2750 1712 2715
Fragments arranged by size:
2750 2715 1712
SfcI C'TryA_G
Cuts at: 826 1045 2015 3720 4525 5660 6341 6532 826
Size: 219 970 1705 805 1135 681 191 1471
Fragments arranged by size:
1705 1471 1135 970 805 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7177
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7177
SmaI CCC'GGG
Cuts at: 1872 4504 1872
Size: 2632 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5281 6182 6444 820
Size: 197 926 3338 901 262 1553
Fragments arranged by size:
3338 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4968 493
Size: 4475 2702
SpeI A'CTAG_T
Cuts at: 152 3152 152
Size: 3000 4177
SphI G_CATG'C
Cuts at: 1132 4535 1132
Size: 3403 3774
SspI AAT'ATT
Cuts at: 5 52 3463 3567 5101 5
Size: 47 3411 104 1534 2081
Fragments arranged by size:
3411 2081 1534 104 47
StuI AGG'CCT
Cuts at: 1517 3356 3710 1517
Size: 1839 354 4984
Fragments arranged by size:
4984 1839 354
StyI C'CwwG_G
Cuts at: 513 1065 3171 4616 513
Size: 552 2106 1445 3074
Fragments arranged by size:
3074 2106 1445 552
SwaI ATTT'AAAT
Cuts at: 2509 2509
Size: 7177
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5204 5221 5374 5559 6004 325
Size: 1255 3624 17 153 185 445 1498
Fragments arranged by size:
3624 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2107 2201 2673
Size: 274 80 33 51 1443 130 94 472
Cuts at: 2673 3074 3546 5423 7135 96
Size: 401 472 1877 1712 138
Fragments arranged by size:
1877 1712 1443 472 472 401 274 138
130 94 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3198 3230 7137 7169 3198
Size: 32 3907 32 3206
Fragments arranged by size:
3907 3206 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7177
XbaI T'CTAG_A
Cuts at: 4513 4513
Size: 7177
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3494 3494
Size: 7177
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7177
XmaI C'CCGG_G
Cuts at: 1870 4502 1870
Size: 2632 4545
XmnI GAAnn'nnTTC
Cuts at: 3136 3731 5306 3136
Size: 595 1575 5007
Fragments arranged by size:
5007 1575 595
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4985 276
Size: 53 83 186 936 3451 2468
Fragments arranged by size:
3451 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI
PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI SwaI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BbeI BbvCI BsiWI
BsmBI BstZ17I EcoICRI EcoNI FseI FspAI KasI NaeI
NarI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI