Restriction map for
pFN21AB3305
(Circular) (Six-base) MAPSORT of: pFN21AB3305.seq Check: 6697 from: 1 to: 6436
pFN21AB3305 6436 bp
With 182 enzymes: SgfI *
October 20, 2009 10:44 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2705 3220 2705
Size: 515 5921
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4246 278
Size: 53 83 186 936 2710 2468
Fragments arranged by size:
2710 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3779 1937
Size: 1842 4594
Acc65I G'GTAC_C
Cuts at: 3757 3757
Size: 6436
AclI AA'CG_TT
Cuts at: 1563 4563 4936 1563
Size: 3000 373 3063
Fragments arranged by size:
3063 3000 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2471 2861 2921 4499 5883 6151 1632
Size: 839 390 60 1578 1384 268 1917
Fragments arranged by size:
1917 1578 1384 839 390 268 60
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6239
AflIII A'CryG_T
Cuts at: 1203 3481 5335 6312 1203
Size: 2278 1854 977 1327
Fragments arranged by size:
2278 1854 1327 977
AgeI A'CCGG_T
Cuts at: 5267 5267
Size: 6436
AhdI GACnn_n'nnGTC
Cuts at: 5165 5165
Size: 6436
AleI CACnn'nnGTG
Cuts at: 1166 3480 1166
Size: 2314 4122
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6436
AlwNI CAG_nnn'CTG
Cuts at: 3500 4179 5751 3500
Size: 679 1572 4185
Fragments arranged by size:
4185 1572 679
ApaI G_GGCC'C
Cuts at: 2566 2566
Size: 6436
ApaLI G'TGCA_C
Cuts at: 2741 4493 5649 2741
Size: 1752 1156 3528
Fragments arranged by size:
3528 1752 1156
ApoI r'AATT_y
Cuts at: 1493 2250 3547 3745 3971 4123 6232 1493
Size: 757 1297 198 226 152 2109 1697
Fragments arranged by size:
2109 1697 1297 757 226 198 152
AseI AT'TA_AT
Cuts at: 160 1032 4990 160
Size: 872 3958 1606
Fragments arranged by size:
3958 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6436
AvaI C'yCGr_G
Cuts at: 1870 1943 2523 3212 3761 1870
Size: 73 580 689 549 4545
Fragments arranged by size:
4545 689 580 549 73
BamHI G'GATC_C
Cuts at: 3766 4133 3766
Size: 367 6069
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2224 2502 2608 2627 3257
Size: 325 217 627 437 278 106 19 630
Cuts at: 3257 3757 5212 618
Size: 500 1455 1842
Fragments arranged by size:
1842 1455 630 627 500 437 325 278
217 106 19
BanII G_rGCy'C
Cuts at: 1391 2546 2566 3755 1391
Size: 1155 20 1189 4072
Fragments arranged by size:
4072 1189 1155 20
BbeI G_GCGC'C
Cuts at: 2506 3261 2506
Size: 755 5681
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5465
BbvCI CC'TCA_GC
Cuts at: 3561 3561
Size: 6436
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4627 4661 1903
Size: 34 2690 34 3678
Fragments arranged by size:
3678 2690 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4328 5538 4328
Size: 1210 5226
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6436
BfrBI ATG'CAT
Cuts at: 2797 2797
Size: 6436
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2511 5047 136
Size: 107 122 71 631 605 839 2536 1525
Fragments arranged by size:
2536 1525 839 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2082 6431 2082
Size: 4349 2087
BlpI GC'TnA_GC
Cuts at: 3853 3853
Size: 6436
Bme1580I G_kGCm'C
Cuts at: 1790 2229 2566 2613 2745 4497 5653 1790
Size: 439 337 47 132 1752 1156 2573
Fragments arranged by size:
2573 1752 1156 439 337 132 47
BmgBI CAC'GTC
Cuts at: 1318 2695 1318
Size: 1377 5059
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2158 2611 2674
Size: 416 53 83 399 24 741 453 63
Cuts at: 2674 3186 4167 5125 442
Size: 512 981 958 1753
Fragments arranged by size:
1753 981 958 741 512 453 416 399
83 63 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6436
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2779 2779
Size: 6436
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3488 5096 1239
Size: 2249 1608 2579
Fragments arranged by size:
2579 2249 1608
Bpu10I CC'TnA_GC
Cuts at: 2808 3402 3561 2808
Size: 594 159 5683
Fragments arranged by size:
5683 594 159
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3410 4561 5426 5724 3410
Size: 1151 865 298 4122
Fragments arranged by size:
4122 1151 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2461 3464 3573 5099 882
Size: 617 962 1003 109 1526 2219
Fragments arranged by size:
2219 1526 1003 962 617 109
BsaAI yAC'GTr
Cuts at: 493 1204 3482 4227 493
Size: 711 2278 745 2702
Fragments arranged by size:
2702 2278 745 711
BsaBI GATnn'nnATC
Cuts at: 6430 6430
Size: 6436
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2503 2872 3258 4243
Size: 53 83 186 936 970 369 386 985
Cuts at: 4243 4625 5260 275
Size: 382 635 1451
Fragments arranged by size:
1451 985 970 936 635 386 382 369
186 83 53
BsaWI w'CCGG_w
Cuts at: 4869 5267 5541 5688 6099 4869
Size: 398 274 147 411 5206
Fragments arranged by size:
5206 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2051 2069 2081 2099 2262
Size: 30 165 30 648 18 12 18 163
Cuts at: 2262 2292 1178
Size: 30 5322
Fragments arranged by size:
5322 648 165 163 30 30 30 18
18 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2755 2932 3446 1186
Size: 1569 177 514 4176
Fragments arranged by size:
4176 1569 514 177
BseYI C'CCAG_C
Cuts at: 2274 2566 3133 3164 5639 6135 2274
Size: 292 567 31 2475 496 2575
Fragments arranged by size:
2575 2475 567 496 292 31
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2739 3248 1163
Size: 573 1003 509 4351
Fragments arranged by size:
4351 1003 573 509
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3886 4647 5675 1540
Size: 275 186 1885 761 1028 2301
Fragments arranged by size:
2301 1885 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2079 2148 2745 4497 4582 5653 2079
Size: 69 597 1752 85 1071 2862
Fragments arranged by size:
2862 1752 1071 597 85 69
BsmI GAATG_Cn'
Cuts at: 3947 4040 3947
Size: 93 6343
BsmBI CGTCTCn'nnnn_
Cuts at: 2351 2879 2351
Size: 528 5908
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2079 2148 2229 2546 2566 2613 2745
Size: 399 289 69 81 317 20 47 132
Cuts at: 2745 3755 4497 4582 5653 1391
Size: 1010 742 85 1071 2174
Fragments arranged by size:
2174 1071 1010 742 399 317 289 132
85 81 69 47 20
BspEI T'CCGG_A
Cuts at: 6099 6099
Size: 6436
BspHI T'CATG_A
Cuts at: 2412 4325 6055 2412
Size: 1913 1730 2793
Fragments arranged by size:
2793 1913 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2705 3220 3791 844
Size: 1861 515 571 3489
Fragments arranged by size:
3489 1861 571 515
BsrBI CCG'CTC
Cuts at: 1394 1823 4323 1394
Size: 429 2500 3507
Fragments arranged by size:
3507 2500 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4931 5105 65
Size: 1183 3683 174 1396
Fragments arranged by size:
3683 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5080 5267 1700
Size: 110 3270 187 2869
Fragments arranged by size:
3270 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6436
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6436
BssSI C'ACGA_G
Cuts at: 4496 5508 4496
Size: 1012 5424
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6436
BstBI TT'CG_AA
Cuts at: 5240 5240
Size: 6436
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6436
BstXI CCAn_nnnn'nTGG
Cuts at: 3646 3646
Size: 6436
BstYI r'GATC_y
Cuts at: 1972 2082 2844 3660 3766 4133 4518 4535 5987
Size: 110 762 816 106 367 385 17 1452
Cuts at: 5987 6073 6085 6431 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 816 762 385 367 346 110
106 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2754 2754
Size: 6436
BtgI C'CryG_G
Cuts at: 513 1065 2049 513
Size: 552 984 4900
Fragments arranged by size:
4900 984 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2168 487
Size: 683 56 942 4755
Fragments arranged by size:
4755 942 683 56
BtsI GCAGTG_nn'
Cuts at: 2370 3258 3952 4746 4766 2370
Size: 888 694 794 20 4040
Fragments arranged by size:
4040 888 794 694 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6436
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6401
DraI TTT'AAA
Cuts at: 3740 3740
Size: 6436
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5443 809
Size: 507 4127 1802
Fragments arranged by size:
4127 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2095 2425 3502
Size: 54 1425 50 275 18 265 330 1077
Cuts at: 3502 3883 4772 6401 8
Size: 381 889 1629 43
Fragments arranged by size:
1629 1425 1077 889 381 330 275 265
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3883 1537
Size: 275 2071 4090
Fragments arranged by size:
4090 2071 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4366 1353
Size: 3013 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5023 5397 5543 387
Size: 1064 138 3434 374 146 1280
Fragments arranged by size:
3434 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2471 2861 2921 3488 4499 5096 5883
Size: 393 839 390 60 567 1011 597 787
Cuts at: 5883 6151 1239
Size: 268 1524
Fragments arranged by size:
1524 1011 839 787 597 567 393 390
268 60
EcoNI CCTnn'n_nnAGG
Cuts at: 2903 3677 2903
Size: 774 5662
EcoO109I rG'GnC_Cy
Cuts at: 2489 2562 2563 2489
Size: 73 1 6362
Fragments arranged by size:
6362 73 1
EcoRI G'AATT_C
Cuts at: 3745 4123 3745
Size: 378 6058
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2800 2800
Size: 6436
FspI TGC'GCA
Cuts at: 4942 4942
Size: 6436
HaeII r_GCGC'y
Cuts at: 1678 2506 3261 3315 5583 1678
Size: 828 755 54 2268 2531
Fragments arranged by size:
2531 2268 828 755 54
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 5077 5109 5151 5183 1373
Size: 32 135 32 3505 32 42 32 2626
Fragments arranged by size:
3505 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3780 4026 669
Size: 988 281 1842 246 3079
Fragments arranged by size:
3079 1842 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2012 748
Size: 1264 5172
HpaI GTT'AAC
Cuts at: 4026 4026
Size: 6436
KasI G'GCGC_C
Cuts at: 2502 3257 2502
Size: 755 5681
KpnI G_GTAC'C
Cuts at: 3761 3761
Size: 6436
MfeI C'AATT_G
Cuts at: 4035 4035
Size: 6436
MluI A'CGCG_T
Cuts at: 6312 6312
Size: 6436
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2409 2415 5550 5734 1305
Size: 1104 6 3135 184 2007
Fragments arranged by size:
3135 2007 1104 184 6
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2427 10
Size: 54 1425 343 595 4019
Fragments arranged by size:
4019 1425 595 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2738 3480 3482 3633 4394 4753 4912
Size: 648 1572 742 2 151 761 359 159
Cuts at: 4912 518
Size: 2042
Fragments arranged by size:
2042 1572 761 742 648 359 159 151
2
MspA1I CmG'CkG
Cuts at: 1606 2051 2094 2558 2723 3500 3560 3852 4529
Size: 445 43 464 165 777 60 292 677
Cuts at: 4529 5677 5922 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 777 677 464 445 292 245
165 60 43
NarI GG'CG_CC
Cuts at: 2503 3258 2503
Size: 755 5681
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5884
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6436
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6436
NotI GC'GGCC_GC
Cuts at: 3883 3883
Size: 6436
NsiI A_TGCA'T
Cuts at: 2799 2799
Size: 6436
NspI r_CATG'y
Cuts at: 1132 1229 3794 5339 1132
Size: 97 2565 1545 2229
Fragments arranged by size:
2565 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6436
PciI A'CATG_T
Cuts at: 5335 5335
Size: 6436
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2104 2434 2623 3253 1108
Size: 996 330 189 630 4291
Fragments arranged by size:
4291 996 630 330 189
PfoI T'CCnGG_A
Cuts at: 2177 2177
Size: 6436
PmeI GTTT'AAAC
Cuts at: 3740 3740
Size: 6436
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4505 4537 6018 6050 4505
Size: 32 1481 32 4891
Fragments arranged by size:
4891 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2489 2489
Size: 6436
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6436
PsiI TTA'TAA
Cuts at: 4006 4006
Size: 6436
PspOMI G'GGCC_C
Cuts at: 2562 2562
Size: 6436
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2000 2032 2000
Size: 32 6404
PstI C_TGCA'G
Cuts at: 830 3087 3788 830
Size: 2257 701 3478
Fragments arranged by size:
3478 2257 701
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6436
PvuII CAG'CTG
Cuts at: 2723 3500 3560 2723
Size: 777 60 5599
Fragments arranged by size:
5599 777 60
SacII CC_GC'GG
Cuts at: 2052 2052
Size: 6436
SalI G'TCGA_C
Cuts at: 1936 3778 1936
Size: 1842 4594
SbfI CC_TGCA'GG
Cuts at: 3788 3788
Size: 6436
ScaI AGT'ACT
Cuts at: 2292 4684 6396 2292
Size: 2392 1712 2332
Fragments arranged by size:
2392 2332 1712
SexAI A'CCwGG_T
Cuts at: 3603 3603
Size: 6436
SfcI C'TryA_G
Cuts at: 826 1045 2089 3083 3120 3190 3784 4919 5600
Size: 219 1044 994 37 70 594 1135 681
Cuts at: 5600 5791 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1044 994 681 594 219 191
70 37
SfoI GGC'GCC
Cuts at: 2504 3259 2504
Size: 755 5681
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6436
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6436
SmaI CCC'GGG
Cuts at: 1872 3763 1872
Size: 1891 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3425 4540 5441 5703 820
Size: 197 926 1482 1115 901 262 1553
Fragments arranged by size:
1553 1482 1115 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4227 493
Size: 3734 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6436
SphI G_CATG'C
Cuts at: 1132 3794 1132
Size: 2662 3774
SspI AAT'ATT
Cuts at: 5 52 2266 4360 5
Size: 47 2214 2094 2081
Fragments arranged by size:
2214 2094 2081 47
StuI AGG'CCT
Cuts at: 1517 3053 1517
Size: 1536 4900
StyI C'CwwG_G
Cuts at: 513 1065 3450 3875 513
Size: 552 2385 425 3074
Fragments arranged by size:
3074 2385 552 425
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2615 2630 4463 4480 4633 4818 5263
Size: 1255 1035 15 1833 17 153 185 445
Cuts at: 5263 325
Size: 1498
Fragments arranged by size:
1833 1498 1255 1035 445 185 153 17
15
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2290 4682 6394
Size: 274 80 33 51 1443 313 2392 1712
Cuts at: 6394 96
Size: 138
Fragments arranged by size:
2392 1712 1443 313 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2069 2101 2262 2294 3516 3548 6396 6428 2069
Size: 32 161 32 1222 32 2848 32 2077
Fragments arranged by size:
2848 2077 1222 161 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2680 3605 1316
Size: 1364 925 4147
Fragments arranged by size:
4147 1364 925
XbaI T'CTAG_A
Cuts at: 3772 3772
Size: 6436
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2620 3358 2620
Size: 738 5698
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6436
XmaI C'CCGG_G
Cuts at: 1870 3761 1870
Size: 1891 4545
XmnI GAAnn'nnTTC
Cuts at: 4565 4565
Size: 6436
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4244 276
Size: 53 83 186 936 2710 2468
Fragments arranged by size:
2710 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII
SacII SalI SbfI ScaI SexAI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BsiWI BstZ17I DraIII
EcoICRI EcoRV FseI FspAI NaeI NgoMIV NruI PacI
PmlI PspXI RsrII SacI SanDI SapI SfiI SrfI
SwaI