Restriction map for
pFN21AB3294
(Circular) (Six-base) MAPSORT of: pFN21AB3294.seq Check: 4414 from: 1 to: 7555
pFN21AB3294 7555 bp
With 182 enzymes: SgfI *
October 20, 2009 10:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3145 3649 3145
Size: 504 7051
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5365 278
Size: 53 83 186 936 3829 2468
Fragments arranged by size:
3829 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2742 4898 1937
Size: 805 2156 4594
Fragments arranged by size:
4594 2156 805
Acc65I G'GTAC_C
Cuts at: 4876 4876
Size: 7555
AclI AA'CG_TT
Cuts at: 1563 5682 6055 1563
Size: 4119 373 3063
Fragments arranged by size:
4119 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2386 4303 5618 7002 7270 1632
Size: 754 1917 1315 1384 268 1917
Fragments arranged by size:
1917 1917 1384 1315 754 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7358
AflIII A'CryG_T
Cuts at: 1203 3525 3816 4095 4347 6454 7431 1203
Size: 2322 291 279 252 2107 977 1327
Fragments arranged by size:
2322 2107 1327 977 291 279 252
AgeI A'CCGG_T
Cuts at: 6386 6386
Size: 7555
AhdI GACnn_n'nnGTC
Cuts at: 6284 6284
Size: 7555
AleI CACnn'nnGTG
Cuts at: 1166 4220 1166
Size: 3054 4501
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7555
AlwNI CAG_nnn'CTG
Cuts at: 2158 3571 5298 6870 2158
Size: 1413 1727 1572 2843
Fragments arranged by size:
2843 1727 1572 1413
ApaI G_GGCC'C
Cuts at: 4070 4295 4070
Size: 225 7330
ApaLI G'TGCA_C
Cuts at: 3533 4463 5612 6768 3533
Size: 930 1149 1156 4320
Fragments arranged by size:
4320 1156 1149 930
ApoI r'AATT_y
Cuts at: 1493 2263 2449 2879 2955 4378 4664 4864 5090
Size: 770 186 430 76 1423 286 200 226
Cuts at: 5090 5242 7351 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 1423 770 430 286 226 200
186 152 76
AseI AT'TA_AT
Cuts at: 160 1032 6109 160
Size: 872 5077 1606
Fragments arranged by size:
5077 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7555
AvaI C'yCGr_G
Cuts at: 1870 1943 2037 2524 3377 3708 3805 4880 1870
Size: 73 94 487 853 331 97 1075 4545
Fragments arranged by size:
4545 1075 853 487 331 97 94 73
BamHI G'GATC_C
Cuts at: 4885 5252 4885
Size: 367 7188
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2129 2182 3143 3703 3745
Size: 325 217 627 342 53 961 560 42
Cuts at: 3745 4027 4876 6331 618
Size: 282 849 1455 1842
Fragments arranged by size:
1842 1455 961 849 627 560 342 325
282 217 53 42
BanII G_rGCy'C
Cuts at: 1391 2038 3299 4070 4295 4874 1391
Size: 647 1261 771 225 579 4072
Fragments arranged by size:
4072 1261 771 647 579 225
BbeI G_GCGC'C
Cuts at: 3707 4031 3707
Size: 324 7231
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2392 4032 928
Size: 971 493 1640 4451
Fragments arranged by size:
4451 1640 971 493
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3091 3125 5746 5780 1903
Size: 34 1154 34 2621 34 3678
Fragments arranged by size:
3678 2621 1154 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4540 5447 6657 4540
Size: 907 1210 5438
Fragments arranged by size:
5438 1210 907
BclI T'GATC_A
Cuts at: 1548 2691 4104 4510 1548
Size: 1143 1413 406 4593
Fragments arranged by size:
4593 1413 1143 406
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6166 136
Size: 107 122 71 631 605 4494 1525
Fragments arranged by size:
4494 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7550 7550
Size: 7555
BlpI GC'TnA_GC
Cuts at: 2519 4972 2519
Size: 2453 5102
Bme1580I G_kGCm'C
Cuts at: 1790 3537 4070 4295 4467 5616 6772 1790
Size: 1747 533 225 172 1149 1156 2573
Fragments arranged by size:
2573 1747 1156 1149 533 225 172
BmgBI CAC'GTC
Cuts at: 1318 3181 3346 4350 1318
Size: 1863 165 1004 4523
Fragments arranged by size:
4523 1863 1004 165
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5286 6244 442
Size: 416 53 83 399 24 3869 958 1753
Fragments arranged by size:
3869 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7555
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2592 2592
Size: 7555
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2186 2249 2342 3030 3608 4213 4557 6215
Size: 947 63 93 688 578 605 344 1658
Cuts at: 6215 1239
Size: 2579
Fragments arranged by size:
2579 1658 947 688 605 578 344 93
63
Bpu10I CC'TnA_GC
Cuts at: 2151 2899 2151
Size: 748 6807
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5680 6545 6843 5680
Size: 865 298 6392
Fragments arranged by size:
6392 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4760 6218 882
Size: 617 3261 1458 2219
Fragments arranged by size:
3261 2219 1458 617
BsaAI yAC'GTr
Cuts at: 493 1204 3526 5346 493
Size: 711 2322 1820 2702
Fragments arranged by size:
2702 2322 1820 711
BsaBI GATnn'nnATC
Cuts at: 7549 7549
Size: 7555
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2204 3704 3798 4028
Size: 53 83 186 936 671 1500 94 230
Cuts at: 4028 4037 4049 5362 5744 6379 275
Size: 9 12 1313 382 635 1451
Fragments arranged by size:
1500 1451 1313 936 671 635 382 230
186 94 83 53 12 9
BsaWI w'CCGG_w
Cuts at: 2662 4640 5988 6386 6660 6807 7218 2662
Size: 1978 1348 398 274 147 411 2999
Fragments arranged by size:
2999 1978 1348 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4418 4448 1178
Size: 30 165 30 3015 30 4285
Fragments arranged by size:
4285 3015 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2054 2297 3596 4487 4778 1186
Size: 868 243 1299 891 291 3963
Fragments arranged by size:
3963 1299 891 868 291 243
BseYI C'CCAG_C
Cuts at: 6758 7254 6758
Size: 496 7059
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2329 3750 1163
Size: 573 593 1421 4968
Fragments arranged by size:
4968 1421 593 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2278 5005 5766 6794 1540
Size: 275 186 277 2727 761 1028 2301
Fragments arranged by size:
2727 2301 1028 761 277 275 186
BsiHKAI G_wGCw'C
Cuts at: 3537 4143 4175 4229 4283 4467 5616 5701 6772
Size: 606 32 54 54 184 1149 85 1071
Cuts at: 6772 3537
Size: 4320
Fragments arranged by size:
4320 1149 1071 606 184 85 54 54
32
BsmI GAATG_Cn'
Cuts at: 4482 5066 5159 4482
Size: 584 93 6878
Fragments arranged by size:
6878 584 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2038 3299 3537 4070 4143 4175 4229
Size: 399 248 1261 238 533 73 32 54
Cuts at: 4229 4283 4295 4467 4874 5616 5701 6772 1391
Size: 54 12 172 407 742 85 1071 2174
Fragments arranged by size:
2174 1261 1071 742 533 407 399 248
238 172 85 73 54 54 32 12
BspEI T'CCGG_A
Cuts at: 7218 7218
Size: 7555
BspHI T'CATG_A
Cuts at: 5444 7174 5444
Size: 1730 5825
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2338 3145 3649 4910 844
Size: 1494 807 504 1261 3489
Fragments arranged by size:
3489 1494 1261 807 504
BsrBI CCG'CTC
Cuts at: 1394 1823 2088 3546 4020 5442 1394
Size: 429 265 1458 474 1422 3507
Fragments arranged by size:
3507 1458 1422 474 429 265
BsrDI GCAATG_nn'
Cuts at: 65 1248 6050 6224 65
Size: 1183 4802 174 1396
Fragments arranged by size:
4802 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3691 3841 3973 6199 6386 1700
Size: 110 1881 150 132 2226 187 2869
Fragments arranged by size:
2869 2226 1881 187 150 132 110
BsrGI T'GTAC_A
Cuts at: 96 3882 4155 96
Size: 3786 273 3496
Fragments arranged by size:
3786 3496 273
BssHII G'CGCG_C
Cuts at: 1925 3508 3510 3612 3787 3855 3857 1925
Size: 1583 2 102 175 68 2 5623
Fragments arranged by size:
5623 1583 175 102 68 2 2
BssSI C'ACGA_G
Cuts at: 2862 3578 3906 5615 6627 2862
Size: 716 328 1709 1012 3790
Fragments arranged by size:
3790 1709 1012 716 328
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7555
BstBI TT'CG_AA
Cuts at: 6359 6359
Size: 7555
BstEII G'GTnAC_C
Cuts at: 1184 3277 1184
Size: 2093 5462
BstXI CCAn_nnnn'nTGG
Cuts at: 4135 4237 4135
Size: 102 7453
BstYI r'GATC_y
Cuts at: 1972 2041 4384 4679 4885 5252 5637 5654 7106
Size: 69 2343 295 206 367 385 17 1452
Cuts at: 7106 7192 7204 7550 1972
Size: 86 12 346 1977
Fragments arranged by size:
2343 1977 1452 385 367 346 295 206
86 69 17 12
BstZ17I GTA'TAC
Cuts at: 2743 2743
Size: 7555
BtgI C'CryG_G
Cuts at: 513 1065 2004 3315 3330 3987 513
Size: 552 939 1311 15 657 4081
Fragments arranged by size:
4081 1311 939 657 552 15
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3094 4093 487
Size: 683 56 1868 999 3949
Fragments arranged by size:
3949 1868 999 683 56
BtsI GCAGTG_nn'
Cuts at: 3560 3798 4145 4523 5071 5865 5885 3560
Size: 238 347 378 548 794 20 5230
Fragments arranged by size:
5230 794 548 378 347 238 20
ClaI AT'CG_AT
Cuts at: 1554 2274 1554
Size: 720 6835
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3982 4017 529
Size: 35 3418 35 4067
Fragments arranged by size:
4067 3418 35 35
DraI TTT'AAA
Cuts at: 4612 4859 4612
Size: 247 7308
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6562 809
Size: 507 5246 1802
Fragments arranged by size:
5246 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2089 2140 3318
Size: 54 1425 50 275 18 259 51 1178
Cuts at: 3318 3339 3990 4021 4520 5002 5891 7520 8
Size: 21 651 31 499 482 889 1629 43
Fragments arranged by size:
1629 1425 1178 889 651 499 482 275
259 54 51 50 43 31 21 18
EagI C'GGCC_G
Cuts at: 1537 1812 5002 1537
Size: 275 3190 4090
Fragments arranged by size:
4090 3190 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2508 5485 1353
Size: 1155 2977 3423
Fragments arranged by size:
3423 2977 1155
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2189 6142 6516 6662 387
Size: 1064 138 600 3953 374 146 1280
Fragments arranged by size:
3953 1280 1064 600 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2186 2249 2342 2386 3030 3608 4213
Size: 393 554 63 93 44 644 578 605
Cuts at: 4213 4303 4557 5618 6215 7002 7270 1239
Size: 90 254 1061 597 787 268 1524
Fragments arranged by size:
1524 1061 787 644 605 597 578 554
393 268 254 93 90 63 44
EcoNI CCTnn'n_nnAGG
Cuts at: 2228 3917 4472 2228
Size: 1689 555 5311
Fragments arranged by size:
5311 1689 555
EcoO109I rG'GnC_Cy
Cuts at: 2026 2155 2221 4291 4505 2026
Size: 129 66 2070 214 5076
Fragments arranged by size:
5076 2070 214 129 66
EcoRI G'AATT_C
Cuts at: 2263 2449 4864 5242 2263
Size: 186 2415 378 4576
Fragments arranged by size:
4576 2415 378 186
FspI TGC'GCA
Cuts at: 6061 6061
Size: 7555
HaeII r_GCGC'y
Cuts at: 1678 3240 3698 3707 3980 4031 4082 4166 6702
Size: 1562 458 9 273 51 51 84 2536
Cuts at: 6702 1678
Size: 2531
Fragments arranged by size:
2536 2531 1562 458 273 84 51 51
9
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2522 2554 2882 2914 2983
Size: 32 135 32 950 32 328 32 69
Cuts at: 2983 3015 3122 3154 3242 3274 3281 3313 3788
Size: 32 107 32 88 32 7 32 475
Cuts at: 3788 3820 6196 6228 6270 6302 1373
Size: 32 2376 32 42 32 2626
Fragments arranged by size:
2626 2376 950 475 328 135 107 88
69 42 32 32 32 32 32 32
32 32 32 32 32 7
HincII GTy'rAC
Cuts at: 669 1657 1938 4093 4591 4899 5145 669
Size: 988 281 2155 498 308 246 3079
Fragments arranged by size:
3079 2155 988 498 308 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7555
HpaI GTT'AAC
Cuts at: 5145 5145
Size: 7555
KasI G'GCGC_C
Cuts at: 3703 4027 3703
Size: 324 7231
KpnI G_GTAC'C
Cuts at: 4880 4880
Size: 7555
MfeI C'AATT_G
Cuts at: 5154 5154
Size: 7555
MluI A'CGCG_T
Cuts at: 3816 7431 3816
Size: 3615 3940
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3210 6669 6853 1305
Size: 1905 3459 184 2007
Fragments arranged by size:
3459 2007 1905 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4522 10
Size: 54 1425 343 2690 3043
Fragments arranged by size:
3043 2690 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3122 4220 5513 5872 6031 518
Size: 648 1956 1098 1293 359 159 2042
Fragments arranged by size:
2042 1956 1293 1098 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2344 4602 4971 5648 6796 7041 1606
Size: 738 2258 369 677 1148 245 2120
Fragments arranged by size:
2258 2120 1148 738 677 369 245
NaeI GCC'GGC
Cuts at: 3693 3843 3975 3693
Size: 150 132 7273
Fragments arranged by size:
7273 150 132
NarI GG'CG_CC
Cuts at: 3704 4028 3704
Size: 324 7231
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 6064
Fragments arranged by size:
6064 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7555
NgoMIV G'CCGG_C
Cuts at: 3691 3841 3973 3691
Size: 150 132 7273
Fragments arranged by size:
7273 150 132
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7555
NotI GC'GGCC_GC
Cuts at: 5002 5002
Size: 7555
NruI TCG'CGA
Cuts at: 2280 3249 2280
Size: 969 6586
NspI r_CATG'y
Cuts at: 1132 1229 4453 4913 6458 1132
Size: 97 3224 460 1545 2229
Fragments arranged by size:
3224 2229 1545 460 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7555
PciI A'CATG_T
Cuts at: 6454 6454
Size: 7555
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7555
PfoI T'CCnGG_A
Cuts at: 2109 3912 4498 2109
Size: 1803 586 5166
Fragments arranged by size:
5166 1803 586
PmeI GTTT'AAAC
Cuts at: 4859 4859
Size: 7555
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5624 5656 7137 7169 5624
Size: 32 1481 32 6010
Fragments arranged by size:
6010 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2155 2221 2155
Size: 66 7489
PshAI GACnn'nnGTC
Cuts at: 1613 3908 1613
Size: 2295 5260
PsiI TTA'TAA
Cuts at: 5125 5125
Size: 7555
PspOMI G'GGCC_C
Cuts at: 4066 4291 4066
Size: 225 7330
PspXI vC'TCGA_Gb
Cuts at: 3708 3708
Size: 7555
PstI C_TGCA'G
Cuts at: 830 2244 2349 3567 3600 3660 3840 4907 830
Size: 1414 105 1218 33 60 180 1067 3478
Fragments arranged by size:
3478 1414 1218 1067 180 105 60 33
PvuI CG_AT'CG
Cuts at: 2001 2278 2001
Size: 277 7278
PvuII CAG'CTG
Cuts at: 4602 4602
Size: 7555
SalI G'TCGA_C
Cuts at: 1936 4897 1936
Size: 2961 4594
SapI GCTCTTCn'nnn_
Cuts at: 2508 2508
Size: 7555
SbfI CC_TGCA'GG
Cuts at: 4907 4907
Size: 7555
ScaI AGT'ACT
Cuts at: 5803 7515 5803
Size: 1712 5843
SexAI A'CCwGG_T
Cuts at: 4851 4851
Size: 7555
SfcI C'TryA_G
Cuts at: 826 1045 2240 2345 2766 3563 3596 3656 3836
Size: 219 1195 105 421 797 33 60 180
Cuts at: 3836 4903 6038 6719 6910 826
Size: 1067 1135 681 191 1471
Fragments arranged by size:
1471 1195 1135 1067 797 681 421 219
191 180 105 60 33
SfoI GGC'GCC
Cuts at: 3705 4029 3705
Size: 324 7231
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7555
SgrAI Cr'CCGG_yG
Cuts at: 1700 3691 1700
Size: 1991 5564
SmaI CCC'GGG
Cuts at: 1872 2039 4882 1872
Size: 167 2843 4545
Fragments arranged by size:
4545 2843 167
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3708 5659 6560 6822 820
Size: 197 926 1765 1951 901 262 1553
Fragments arranged by size:
1951 1765 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5346 493
Size: 4853 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7555
SphI G_CATG'C
Cuts at: 1132 4453 4913 1132
Size: 3321 460 3774
Fragments arranged by size:
3774 3321 460
SspI AAT'ATT
Cuts at: 5 52 2424 2600 5479 5
Size: 47 2372 176 2879 2081
Fragments arranged by size:
2879 2372 2081 176 47
StuI AGG'CCT
Cuts at: 1517 4329 1517
Size: 2812 4743
StyI C'CwwG_G
Cuts at: 513 1065 2004 4994 513
Size: 552 939 2990 3074
Fragments arranged by size:
3074 2990 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3250 3704 3749 4046 4312 4319 5582
Size: 1255 1670 454 45 297 266 7 1263
Cuts at: 5582 5599 5752 5937 6382 325
Size: 17 153 185 445 1498
Fragments arranged by size:
1670 1498 1263 1255 454 445 297 266
185 153 45 17 7
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3381 3882 4155
Size: 274 80 33 51 1443 1404 501 273
Cuts at: 4155 5801 7513 96
Size: 1646 1712 138
Fragments arranged by size:
1712 1646 1443 1404 501 274 273 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2769 2801 7515 7547 2769
Size: 32 4714 32 2777
Fragments arranged by size:
4714 2777 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7555
XbaI T'CTAG_A
Cuts at: 2438 4891 2438
Size: 2453 5102
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3328 3328
Size: 7555
XhoI C'TCGA_G
Cuts at: 1943 3708 1943
Size: 1765 5790
XmaI C'CCGG_G
Cuts at: 1870 2037 4880 1870
Size: 167 2843 4545
Fragments arranged by size:
4545 2843 167
XmnI GAAnn'nnTTC
Cuts at: 5684 5684
Size: 7555
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5363 276
Size: 53 83 186 936 3829 2468
Fragments arranged by size:
3829 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PspXI PstI PvuI PvuII SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BfrBI BsiWI
BsmBI Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI
NsiI PacI PmlI PsrI RsrII SacI SacII SanDI
SfiI SrfI SwaI