Restriction map for
pFN21AB3282
(Circular) (Six-base) MAPSORT of: pFN21AB3282.seq Check: 8686 from: 1 to: 6925
pFN21AB3282 6925 bp
With 182 enzymes: SgfI *
February 27, 2009 14:46 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4735 278
Size: 53 83 186 936 3199 2468
Fragments arranged by size:
3199 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2544 4268 1937
Size: 607 1724 4594
Fragments arranged by size:
4594 1724 607
Acc65I G'GTAC_C
Cuts at: 2499 4246 2499
Size: 1747 5178
AclI AA'CG_TT
Cuts at: 1563 3013 5052 5425 1563
Size: 1450 2039 373 3063
Fragments arranged by size:
3063 2039 1450 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2512 2945 3550 4988 6372 6640 1632
Size: 880 433 605 1438 1384 268 1917
Fragments arranged by size:
1917 1438 1384 880 605 433 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6728
AflIII A'CryG_T
Cuts at: 1203 5824 6801 1203
Size: 4621 977 1327
Fragments arranged by size:
4621 1327 977
AgeI A'CCGG_T
Cuts at: 5756 5756
Size: 6925
AhdI GACnn_n'nnGTC
Cuts at: 5654 5654
Size: 6925
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6925
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6925
AlwNI CAG_nnn'CTG
Cuts at: 2030 3043 4668 6240 2030
Size: 1013 1625 1572 2715
Fragments arranged by size:
2715 1625 1572 1013
ApaI G_GGCC'C
Cuts at: 2772 2772
Size: 6925
ApaLI G'TGCA_C
Cuts at: 2888 4982 6138 2888
Size: 2094 1156 3675
Fragments arranged by size:
3675 2094 1156
ApoI r'AATT_y
Cuts at: 1493 2283 2834 3184 3570 3969 4131 4170 4234
Size: 790 551 350 386 399 162 39 64
Cuts at: 4234 4460 4612 6721 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 790 551 399 386 350 226
162 152 64 39
AseI AT'TA_AT
Cuts at: 160 1032 2444 5479 160
Size: 872 1412 3035 1606
Fragments arranged by size:
3035 1606 1412 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6925
AvaI C'yCGr_G
Cuts at: 1870 1943 2893 3267 4250 1870
Size: 73 950 374 983 4545
Fragments arranged by size:
4545 983 950 374 73
BamHI G'GATC_C
Cuts at: 4255 4622 4255
Size: 367 6558
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2499 4246 5701 618
Size: 325 217 627 712 1747 1455 1842
Fragments arranged by size:
1842 1747 1455 712 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2772 3585 4244 1391
Size: 1381 813 659 4072
Fragments arranged by size:
4072 1381 813 659
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3219 3665 4188 928
Size: 971 1320 446 523 3665
Fragments arranged by size:
3665 1320 971 523 446
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3913 3947 5116 5150 1903
Size: 34 1976 34 1169 34 3678
Fragments arranged by size:
3678 1976 1169 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3761 4063 4817 6027 3761
Size: 302 754 1210 4659
Fragments arranged by size:
4659 1210 754 302
BclI T'GATC_A
Cuts at: 1548 2592 1548
Size: 1044 5881
BfrBI ATG'CAT
Cuts at: 3520 3715 3520
Size: 195 6730
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5536 136
Size: 107 122 71 631 605 3864 1525
Fragments arranged by size:
3864 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3739 6920 3739
Size: 3181 3744
BlpI GC'TnA_GC
Cuts at: 4342 4342
Size: 6925
Bme1580I G_kGCm'C
Cuts at: 1790 2772 2892 4986 6142 1790
Size: 982 120 2094 1156 2573
Fragments arranged by size:
2573 2094 1156 982 120
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6925
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4656 5614 442
Size: 416 53 83 399 24 3239 958 1753
Fragments arranged by size:
3239 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 4127 1056
Size: 3071 3854
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2259 3219 5585 1239
Size: 1020 960 2366 2579
Fragments arranged by size:
2579 2366 1020 960
Bpu10I CC'TnA_GC
Cuts at: 3369 3369
Size: 6925
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2335 5050 5915 6213 2335
Size: 2715 865 298 3047
Fragments arranged by size:
3047 2715 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2207 5588 882
Size: 617 708 3381 2219
Fragments arranged by size:
3381 2219 708 617
BsaAI yAC'GTr
Cuts at: 493 1204 4716 493
Size: 711 3512 2702
Fragments arranged by size:
3512 2702 711
BsaBI GATnn'nnATC
Cuts at: 2465 3426 6919 2465
Size: 961 3493 2471
Fragments arranged by size:
3493 2471 961
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4732 5114 5749 275
Size: 53 83 186 936 3199 382 635 1451
Fragments arranged by size:
3199 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5358 5756 6030 6177 6588 5358
Size: 398 274 147 411 5695
Fragments arranged by size:
5695 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3052 3082 3357 3387 3465
Size: 30 165 30 1649 30 275 30 78
Cuts at: 3465 3495 1178
Size: 30 4608
Fragments arranged by size:
4608 1649 275 165 78 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2766 3612 1186
Size: 1580 846 4499
Fragments arranged by size:
4499 1580 846
BseYI C'CCAG_C
Cuts at: 6128 6624 6128
Size: 496 6429
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2315 1163
Size: 573 579 5773
Fragments arranged by size:
5773 579 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4375 5136 6164 1540
Size: 275 186 2374 761 1028 2301
Fragments arranged by size:
2374 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2892 4986 5071 6142 2892
Size: 2094 85 1071 3675
Fragments arranged by size:
3675 2094 1071 85
BsmI GAATG_Cn'
Cuts at: 2251 2513 3522 4436 4529 2251
Size: 262 1009 914 93 4647
Fragments arranged by size:
4647 1009 914 262 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2772 2892 3585 4244 4986 5071 6142
Size: 399 982 120 693 659 742 85 1071
Cuts at: 6142 1391
Size: 2174
Fragments arranged by size:
2174 1071 982 742 693 659 399 120
85
BspEI T'CCGG_A
Cuts at: 6588 6588
Size: 6925
BspHI T'CATG_A
Cuts at: 3027 4814 6544 3027
Size: 1787 1730 3408
Fragments arranged by size:
3408 1787 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4280 844
Size: 3436 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4812 1394
Size: 429 2989 3507
Fragments arranged by size:
3507 2989 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2203 5420 5594 65
Size: 1183 955 3217 174 1396
Fragments arranged by size:
3217 1396 1183 955 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5569 5756 1700
Size: 110 3759 187 2869
Fragments arranged by size:
3759 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3273 96
Size: 3177 3748
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6925
BssSI C'ACGA_G
Cuts at: 4985 5997 4985
Size: 1012 5913
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6925
BstBI TT'CG_AA
Cuts at: 5729 5729
Size: 6925
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6925
BstYI r'GATC_y
Cuts at: 1972 3739 4255 4622 5007 5024 6476 6562 6574
Size: 1767 516 367 385 17 1452 86 12
Cuts at: 6574 6920 1972
Size: 346 1977
Fragments arranged by size:
1977 1767 1452 516 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2149 513
Size: 552 1084 5289
Fragments arranged by size:
5289 1084 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6186
Fragments arranged by size:
6186 683 56
BtsI GCAGTG_nn'
Cuts at: 2802 3073 3099 3174 3261 4441 5235 5255 2802
Size: 271 26 75 87 1180 794 20 4472
Fragments arranged by size:
4472 1180 794 271 87 75 26 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6925
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3529 3564 529
Size: 35 2965 35 3890
Fragments arranged by size:
3890 2965 35 35
DraI TTT'AAA
Cuts at: 2136 4229 2136
Size: 2093 4832
DraIII CAC_nnn'GTG
Cuts at: 2338 2338
Size: 6925
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5932 809
Size: 507 4616 1802
Fragments arranged by size:
4616 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2146 3130 4372
Size: 54 1425 50 275 18 316 984 1242
Cuts at: 4372 5261 6890 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1242 984 889 316 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4372 1537
Size: 275 2560 4090
Fragments arranged by size:
4090 2560 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3569 4855 1353
Size: 2216 1286 3423
Fragments arranged by size:
3423 2216 1286
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5512 5886 6032 387
Size: 1064 138 3923 374 146 1280
Fragments arranged by size:
3923 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2259 2512 2945 3219 3550 4988 5585
Size: 393 627 253 433 274 331 1438 597
Cuts at: 5585 6372 6640 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1438 787 627 597 433 393 331
274 268 253
EcoRI G'AATT_C
Cuts at: 3570 4131 4234 4612 3570
Size: 561 103 378 5883
Fragments arranged by size:
5883 561 378 103
FspI TGC'GCA
Cuts at: 5431 5431
Size: 6925
HaeII r_GCGC'y
Cuts at: 1678 4057 6072 1678
Size: 2379 2015 2531
Fragments arranged by size:
2531 2379 2015
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3520 3552 3815 3847 4205
Size: 32 135 32 1948 32 263 32 358
Cuts at: 4205 4237 5566 5598 5640 5672 1373
Size: 32 1329 32 42 32 2626
Fragments arranged by size:
2626 1948 1329 358 263 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2485 4269 4515 669
Size: 988 281 547 1784 246 3079
Fragments arranged by size:
3079 1784 988 547 281 246
HindIII A'AGCT_T
Cuts at: 748 2976 748
Size: 2228 4697
HpaI GTT'AAC
Cuts at: 4515 4515
Size: 6925
KpnI G_GTAC'C
Cuts at: 2503 4250 2503
Size: 1747 5178
MfeI C'AATT_G
Cuts at: 2763 4064 4524 2763
Size: 1301 460 5164
Fragments arranged by size:
5164 1301 460
MluI A'CGCG_T
Cuts at: 6801 6801
Size: 6925
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6039 6223 1305
Size: 4734 184 2007
Fragments arranged by size:
4734 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2148 3132 10
Size: 54 1425 343 316 984 3803
Fragments arranged by size:
3803 1425 984 343 316 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2553 4883 5242 5401 518
Size: 648 1387 2330 359 159 2042
Fragments arranged by size:
2330 2042 1387 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2030 4341 5018 6166 6411 1606
Size: 424 2311 677 1148 245 2120
Fragments arranged by size:
2311 2120 1148 677 424 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6373
NdeI CA'TA_TG
Cuts at: 387 2013 2095 3630 387
Size: 1626 82 1535 3682
Fragments arranged by size:
3682 1626 1535 82
NheI G'CTAG_C
Cuts at: 1052 4123 1052
Size: 3071 3854
NotI GC'GGCC_GC
Cuts at: 4372 4372
Size: 6925
NsiI A_TGCA'T
Cuts at: 3522 3717 3522
Size: 195 6730
NspI r_CATG'y
Cuts at: 1132 1229 4283 5828 1132
Size: 97 3054 1545 2229
Fragments arranged by size:
3054 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6925
PciI A'CATG_T
Cuts at: 5824 5824
Size: 6925
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2665 3742 1108
Size: 1557 1077 4291
Fragments arranged by size:
4291 1557 1077
PmeI GTTT'AAAC
Cuts at: 4229 4229
Size: 6925
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4994 5026 6507 6539 4994
Size: 32 1481 32 5380
Fragments arranged by size:
5380 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6925
PsiI TTA'TAA
Cuts at: 2586 3318 4495 2586
Size: 732 1177 5016
Fragments arranged by size:
5016 1177 732
PspOMI G'GGCC_C
Cuts at: 2768 2768
Size: 6925
PspXI vC'TCGA_Gb
Cuts at: 3267 3267
Size: 6925
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3738 3770 3738
Size: 32 6893
PstI C_TGCA'G
Cuts at: 830 2185 4277 830
Size: 1355 2092 3478
Fragments arranged by size:
3478 2092 1355
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6925
PvuII CAG'CTG
Cuts at: 2030 2030
Size: 6925
SalI G'TCGA_C
Cuts at: 1936 4267 1936
Size: 2331 4594
SbfI CC_TGCA'GG
Cuts at: 4277 4277
Size: 6925
ScaI AGT'ACT
Cuts at: 5173 6885 5173
Size: 1712 5213
SfcI C'TryA_G
Cuts at: 826 1045 2181 4273 5408 6089 6280 826
Size: 219 1136 2092 1135 681 191 1471
Fragments arranged by size:
2092 1471 1136 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6925
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6925
SmaI CCC'GGG
Cuts at: 1872 4252 1872
Size: 2380 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2350 2893 3267 5029 5930 6192
Size: 197 926 407 543 374 1762 901 262
Cuts at: 6192 820
Size: 1553
Fragments arranged by size:
1762 1553 926 901 543 407 374 262
197
SnaBI TAC'GTA
Cuts at: 493 4716 493
Size: 4223 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6925
SphI G_CATG'C
Cuts at: 1132 4283 1132
Size: 3151 3774
SspI AAT'ATT
Cuts at: 5 52 4849 5
Size: 47 4797 2081
Fragments arranged by size:
4797 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6925
StyI C'CwwG_G
Cuts at: 513 1065 4364 513
Size: 552 3299 3074
Fragments arranged by size:
3299 3074 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4952 4969 5122 5307 5752 325
Size: 1255 3372 17 153 185 445 1498
Fragments arranged by size:
3372 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3273 5171 6883
Size: 274 80 33 51 1443 1296 1898 1712
Cuts at: 6883 96
Size: 138
Fragments arranged by size:
1898 1712 1443 1296 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2382 2414 2663 2695 2778 2810 3052 3084 3465
Size: 32 249 32 83 32 242 32 381
Cuts at: 3465 3497 6885 6917 2382
Size: 32 3388 32 2390
Fragments arranged by size:
3388 2390 381 249 242 83 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6925
XbaI T'CTAG_A
Cuts at: 4261 4261
Size: 6925
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3359 3359
Size: 6925
XhoI C'TCGA_G
Cuts at: 1943 2893 3267 1943
Size: 950 374 5601
Fragments arranged by size:
5601 950 374
XmaI C'CCGG_G
Cuts at: 1870 4250 1870
Size: 2380 4545
XmnI GAAnn'nnTTC
Cuts at: 3450 5054 3450
Size: 1604 5321
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4733 276
Size: 53 83 186 936 3199 2468
Fragments arranged by size:
3199 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI PvuII SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI
EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI PacI PfoI PmlI PpuMI RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI