Restriction map for
pFN21AB3281
(Circular) (Six-base) MAPSORT of: pFN21AB3281.seq Check: 6716 from: 1 to: 7066
pFN21AB3281 7066 bp
With 182 enzymes: SgfI *
February 27, 2009 14:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2617 2617
Size: 7066
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4876 278
Size: 53 83 186 936 3340 2468
Fragments arranged by size:
3340 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4038 4409 1937
Size: 2101 371 4594
Fragments arranged by size:
4594 2101 371
Acc65I G'GTAC_C
Cuts at: 4387 4387
Size: 7066
AclI AA'CG_TT
Cuts at: 1563 5193 5566 1563
Size: 3630 373 3063
Fragments arranged by size:
3630 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2273 3050 3235 5129 6513 6781 1632
Size: 641 777 185 1894 1384 268 1917
Fragments arranged by size:
1917 1894 1384 777 641 268 185
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6869
AflIII A'CryG_T
Cuts at: 1203 2292 4008 4227 5965 6942 1203
Size: 1089 1716 219 1738 977 1327
Fragments arranged by size:
1738 1716 1327 1089 977 219
AgeI A'CCGG_T
Cuts at: 5897 5897
Size: 7066
AhdI GACnn_n'nnGTC
Cuts at: 5795 5795
Size: 7066
AleI CACnn'nnGTG
Cuts at: 1166 3523 1166
Size: 2357 4709
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7066
AlwNI CAG_nnn'CTG
Cuts at: 2185 2206 2668 2722 2785 2911 3028 3056 3476
Size: 21 462 54 63 126 117 28 420
Cuts at: 3476 3613 4809 6381 2185
Size: 137 1196 1572 2870
Fragments arranged by size:
2870 1572 1196 462 420 137 126 117
63 54 28 21
ApaI G_GGCC'C
Cuts at: 2805 2805
Size: 7066
ApaLI G'TGCA_C
Cuts at: 3089 5123 6279 3089
Size: 2034 1156 3876
Fragments arranged by size:
3876 2034 1156
ApoI r'AATT_y
Cuts at: 1493 3320 3545 3688 4375 4601 4753 6862 1493
Size: 1827 225 143 687 226 152 2109 1697
Fragments arranged by size:
2109 1827 1697 687 226 225 152 143
AseI AT'TA_AT
Cuts at: 160 1032 5620 160
Size: 872 4588 1606
Fragments arranged by size:
4588 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7066
AvaI C'yCGr_G
Cuts at: 1870 1943 2807 4391 1870
Size: 73 864 1584 4545
Fragments arranged by size:
4545 1584 864 73
BamHI G'GATC_C
Cuts at: 2547 4347 4396 4763 2547
Size: 1800 49 367 4850
Fragments arranged by size:
4850 1800 367 49
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3233 3484 4387 5842 618
Size: 325 217 627 1446 251 903 1455 1842
Fragments arranged by size:
1842 1455 1446 903 627 325 251 217
BanII G_rGCy'C
Cuts at: 1391 2585 2805 4320 4385 1391
Size: 1194 220 1515 65 4072
Fragments arranged by size:
4072 1515 1194 220 65
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2714 3038 928
Size: 971 815 324 4956
Fragments arranged by size:
4956 971 815 324
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5257 5291 1903
Size: 34 3320 34 3678
Fragments arranged by size:
3678 3320 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2010 4091 4958 6168 2010
Size: 2081 867 1210 2908
Fragments arranged by size:
2908 2081 1210 867
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7066
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5677 136
Size: 107 122 71 631 605 4005 1525
Fragments arranged by size:
4005 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2143 3189 7061 2143
Size: 1046 3872 2148
Fragments arranged by size:
3872 2148 1046
BlpI GC'TnA_GC
Cuts at: 2025 2579 2892 4483 2025
Size: 554 313 1591 4608
Fragments arranged by size:
4608 1591 554 313
Bme1580I G_kGCm'C
Cuts at: 1790 2271 2805 3093 3247 5127 6283 1790
Size: 481 534 288 154 1880 1156 2573
Fragments arranged by size:
2573 1880 1156 534 481 288 154
BmgBI CAC'GTC
Cuts at: 1318 2824 1318
Size: 1506 5560
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2104 2921 3337
Size: 416 53 83 399 24 687 817 416
Cuts at: 3337 3667 4797 5755 442
Size: 330 1130 958 1753
Fragments arranged by size:
1753 1130 958 817 687 416 416 399
330 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7066
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2207 2270 2373 2414 2600 2657 3659 4301
Size: 968 63 103 41 186 57 1002 642
Cuts at: 4301 5726 1239
Size: 1425 2579
Fragments arranged by size:
2579 1425 1002 968 642 186 103 63
57 41
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3137 3743 5191 6056 6354 3137
Size: 606 1448 865 298 3849
Fragments arranged by size:
3849 1448 865 606 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5729 882
Size: 617 4230 2219
Fragments arranged by size:
4230 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2295 4009 4102 4176 4857 493
Size: 711 1091 1714 93 74 681 2702
Fragments arranged by size:
2702 1714 1091 711 681 93 74
BsaBI GATnn'nnATC
Cuts at: 7060 7060
Size: 7066
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4873 5255 5890 275
Size: 53 83 186 936 3340 382 635 1451
Fragments arranged by size:
3340 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5499 5897 6171 6318 6729 5499
Size: 398 274 147 411 5836
Fragments arranged by size:
5836 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2630 2660 1178
Size: 30 165 30 1227 30 5584
Fragments arranged by size:
5584 1227 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2465 2948 3009 4328 1186
Size: 1279 483 61 1319 3924
Fragments arranged by size:
3924 1319 1279 483 61
BseYI C'CCAG_C
Cuts at: 2178 2263 2574 4198 6269 6765 2178
Size: 85 311 1624 2071 496 2479
Fragments arranged by size:
2479 2071 1624 496 311 85
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2219 3581 1163
Size: 573 483 1362 4648
Fragments arranged by size:
4648 1362 573 483
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3979 4516 5277 6305 1540
Size: 275 186 1978 537 761 1028 2301
Fragments arranged by size:
2301 1978 1028 761 537 275 186
BsiHKAI G_wGCw'C
Cuts at: 2226 2898 3093 4320 5127 5212 6283 2226
Size: 672 195 1227 807 85 1071 3009
Fragments arranged by size:
3009 1227 1071 807 672 195 85
BsmI GAATG_Cn'
Cuts at: 4577 4670 4577
Size: 93 6973
BsmBI CGTCTCn'nnnn_
Cuts at: 2600 2600
Size: 7066
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2226 2271 2585 2805 2898 3093 3247
Size: 399 436 45 314 220 93 195 154
Cuts at: 3247 4320 4385 5127 5212 6283 1391
Size: 1073 65 742 85 1071 2174
Fragments arranged by size:
2174 1073 1071 742 436 399 314 220
195 154 93 85 65 45
BspEI T'CCGG_A
Cuts at: 6729 6729
Size: 7066
BspHI T'CATG_A
Cuts at: 4955 6685 4955
Size: 1730 5336
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2617 4421 844
Size: 1773 1804 3489
Fragments arranged by size:
3489 1804 1773
BsrBI CCG'CTC
Cuts at: 1394 1823 4953 1394
Size: 429 3130 3507
Fragments arranged by size:
3507 3130 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3178 3725 3888 5561 5735 65
Size: 1183 1930 547 163 1673 174 1396
Fragments arranged by size:
1930 1673 1396 1183 547 174 163
BsrFI r'CCGG_y
Cuts at: 1700 1810 2871 4326 5710 5897 1700
Size: 110 1061 1455 1384 187 2869
Fragments arranged by size:
2869 1455 1384 1061 187 110
BsrGI T'GTAC_A
Cuts at: 96 2343 96
Size: 2247 4819
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7066
BssSI C'ACGA_G
Cuts at: 3593 5126 6138 3593
Size: 1533 1012 4521
Fragments arranged by size:
4521 1533 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2206 2722 794
Size: 1412 516 5138
Fragments arranged by size:
5138 1412 516
BstBI TT'CG_AA
Cuts at: 5870 5870
Size: 7066
BstEII G'GTnAC_C
Cuts at: 1184 2986 1184
Size: 1802 5264
BstXI CCAn_nnnn'nTGG
Cuts at: 2317 2746 4180 2317
Size: 429 1434 5203
Fragments arranged by size:
5203 1434 429
BstYI r'GATC_y
Cuts at: 1972 2143 2547 2880 3189 4347 4396 4763 5148
Size: 171 404 333 309 1158 49 367 385
Cuts at: 5148 5165 6617 6703 6715 7061 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1158 404 385 367 346 333
309 171 86 49 17 12
BstZ17I GTA'TAC
Cuts at: 4039 4039
Size: 7066
Bsu36I CC'TnA_GG
Cuts at: 2543 3021 2543
Size: 478 6588
BtgI C'CryG_G
Cuts at: 513 1065 2004 2310 513
Size: 552 939 306 5269
Fragments arranged by size:
5269 939 552 306
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6327
Fragments arranged by size:
6327 683 56
BtsI GCAGTG_nn'
Cuts at: 2666 4582 5376 5396 2666
Size: 1916 794 20 4336
Fragments arranged by size:
4336 1916 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7066
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7031
DraI TTT'AAA
Cuts at: 4370 4370
Size: 7066
DraIII CAC_nnn'GTG
Cuts at: 2230 2230
Size: 7066
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6073 809
Size: 507 4757 1802
Fragments arranged by size:
4757 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2071 2691 2787
Size: 54 1425 50 275 18 241 620 96
Cuts at: 2787 2850 2943 3441 3515 4022 4328 4513 5402
Size: 63 93 498 74 507 306 185 889
Cuts at: 5402 7031 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 620 507 498 306 275
241 185 96 93 74 63 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4513 1537
Size: 275 2701 4090
Fragments arranged by size:
4090 2701 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4996 1353
Size: 3643 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2321 5653 6027 6173 387
Size: 1064 138 732 3332 374 146 1280
Fragments arranged by size:
3332 1280 1064 732 374 146 138
EcoICRI GAG'CTC
Cuts at: 4318 4318
Size: 7066
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2207 2270 2273 2373 2414 2600 2657
Size: 393 575 63 3 100 41 186 57
Cuts at: 2657 3050 3235 3659 4301 5129 5726 6513 6781
Size: 393 185 424 642 828 597 787 268
Cuts at: 6781 1239
Size: 1524
Fragments arranged by size:
1524 828 787 642 597 575 424 393
393 268 186 185 100 63 57 41
3
EcoNI CCTnn'n_nnAGG
Cuts at: 2198 3581 2198
Size: 1383 5683
EcoO109I rG'GnC_Cy
Cuts at: 2191 2802 3025 3574 3843 4189 2191
Size: 611 223 549 269 346 5068
Fragments arranged by size:
5068 611 549 346 269 223
EcoRI G'AATT_C
Cuts at: 4375 4753 4375
Size: 378 6688
FseI GG_CCGG'CC
Cuts at: 4330 4330
Size: 7066
FspI TGC'GCA
Cuts at: 5572 5572
Size: 7066
HaeII r_GCGC'y
Cuts at: 1678 3536 4173 6213 1678
Size: 1858 637 2040 2531
Fragments arranged by size:
2531 2040 1858 637
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2008 2040 2399 2431 2539
Size: 32 135 32 436 32 359 32 108
Cuts at: 2539 2571 2960 2992 5707 5739 5781 5813 1373
Size: 32 389 32 2715 32 42 32 2626
Fragments arranged by size:
2715 2626 436 389 359 135 108 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2953 4410 4656 669
Size: 988 281 1015 1457 246 3079
Fragments arranged by size:
3079 1457 1015 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7066
HpaI GTT'AAC
Cuts at: 4656 4656
Size: 7066
KpnI G_GTAC'C
Cuts at: 4391 4391
Size: 7066
MfeI C'AATT_G
Cuts at: 4665 4665
Size: 7066
MluI A'CGCG_T
Cuts at: 6942 6942
Size: 7066
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6180 6364 1305
Size: 4875 184 2007
Fragments arranged by size:
4875 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2073 3517 10
Size: 54 1425 343 241 1444 3559
Fragments arranged by size:
3559 1444 1425 343 241 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2090 3523 3771 4178 5024 5383
Size: 648 843 81 1433 248 407 846 359
Cuts at: 5383 5542 518
Size: 159 2042
Fragments arranged by size:
2042 1433 846 843 648 407 359 248
159 81
MspA1I CmG'CkG
Cuts at: 1606 2304 2325 2393 2569 2578 2719 2785 2911
Size: 698 21 68 176 9 141 66 126
Cuts at: 2911 3523 4202 4482 5159 6307 6552 1606
Size: 612 679 280 677 1148 245 2120
Fragments arranged by size:
2120 1148 698 679 677 612 280 245
176 141 126 68 66 21 9
NaeI GCC'GGC
Cuts at: 4328 4328
Size: 7066
NcoI C'CATG_G
Cuts at: 513 1065 2004 2310 513
Size: 552 939 306 5269
Fragments arranged by size:
5269 939 552 306
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7066
NgoMIV G'CCGG_C
Cuts at: 4326 4326
Size: 7066
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7066
NotI GC'GGCC_GC
Cuts at: 4513 4513
Size: 7066
NspI r_CATG'y
Cuts at: 1132 1229 4231 4424 5969 1132
Size: 97 3002 193 1545 2229
Fragments arranged by size:
3002 2229 1545 193 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7066
PciI A'CATG_T
Cuts at: 4227 5965 4227
Size: 1738 5328
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2185 3894 1108
Size: 1077 1709 4280
Fragments arranged by size:
4280 1709 1077
PfoI T'CCnGG_A
Cuts at: 2184 2745 2184
Size: 561 6505
PmeI GTTT'AAAC
Cuts at: 4370 4370
Size: 7066
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4232 4264 5135 5167 6648 6680 4232
Size: 32 871 32 1481 32 4618
Fragments arranged by size:
4618 1481 871 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3025 3843 3025
Size: 818 6248
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7066
PsiI TTA'TAA
Cuts at: 4636 4636
Size: 7066
PspOMI G'GGCC_C
Cuts at: 2801 2801
Size: 7066
PstI C_TGCA'G
Cuts at: 830 2211 2502 2673 2718 4153 4418 830
Size: 1381 291 171 45 1435 265 3478
Fragments arranged by size:
3478 1435 1381 291 265 171 45
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7066
PvuII CAG'CTG
Cuts at: 2393 2578 2719 2785 2911 3523 4202 2393
Size: 185 141 66 126 612 679 5257
Fragments arranged by size:
5257 679 612 185 141 126 66
SacI G_AGCT'C
Cuts at: 4320 4320
Size: 7066
SalI G'TCGA_C
Cuts at: 1936 4408 1936
Size: 2472 4594
SbfI CC_TGCA'GG
Cuts at: 4418 4418
Size: 7066
ScaI AGT'ACT
Cuts at: 2516 5314 7026 2516
Size: 2798 1712 2556
Fragments arranged by size:
2798 2556 1712
SexAI A'CCwGG_T
Cuts at: 2226 2226
Size: 7066
SfcI C'TryA_G
Cuts at: 826 1045 2207 2498 2669 2714 2828 3646 4149
Size: 219 1162 291 171 45 114 818 503
Cuts at: 4149 4414 5549 6230 6421 826
Size: 265 1135 681 191 1471
Fragments arranged by size:
1471 1162 1135 818 681 503 291 265
219 191 171 114 45
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7066
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7066
SmaI CCC'GGG
Cuts at: 1872 4393 1872
Size: 2521 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3152 3758 5170 6071 6333 820
Size: 197 926 1209 606 1412 901 262 1553
Fragments arranged by size:
1553 1412 1209 926 901 606 262 197
SnaBI TAC'GTA
Cuts at: 493 4857 493
Size: 4364 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7066
SphI G_CATG'C
Cuts at: 1132 4424 1132
Size: 3292 3774
SspI AAT'ATT
Cuts at: 5 52 4990 5
Size: 47 4938 2081
Fragments arranged by size:
4938 2081 47
StuI AGG'CCT
Cuts at: 1517 2642 1517
Size: 1125 5941
StyI C'CwwG_G
Cuts at: 513 1065 2004 2035 2310 3582 4505 513
Size: 552 939 31 275 1272 923 3074
Fragments arranged by size:
3074 1272 939 923 552 275 31
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2192 3954 5093 5110 5263 5448 5893
Size: 1255 612 1762 1139 17 153 185 445
Cuts at: 5893 325
Size: 1498
Fragments arranged by size:
1762 1498 1255 1139 612 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2343 2514 5312
Size: 274 80 33 51 1443 366 171 2798
Cuts at: 5312 7024 96
Size: 1712 138
Fragments arranged by size:
2798 1712 1443 366 274 171 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4045 4077 4328 4360 7026 7058 4045
Size: 32 251 32 2666 32 4053
Fragments arranged by size:
4053 2666 251 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3388 4250 1316
Size: 2072 862 4132
Fragments arranged by size:
4132 2072 862
XbaI T'CTAG_A
Cuts at: 4402 4402
Size: 7066
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2182 2558 3350 2182
Size: 376 792 5898
Fragments arranged by size:
5898 792 376
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7066
XmaI C'CCGG_G
Cuts at: 1870 4391 1870
Size: 2521 4545
XmnI GAAnn'nnTTC
Cuts at: 2046 3614 5195 2046
Size: 1568 1581 3917
Fragments arranged by size:
3917 1581 1568
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4874 276
Size: 53 83 186 936 3340 2468
Fragments arranged by size:
3340 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI EcoRV FalI FspAI KasI NarI
NruI NsiI PacI PmlI PspXI PsrI RsrII SacII
SanDI SapI SfiI SfoI SrfI SwaI