Restriction map for
pFN21AB3278
(Circular) (Six-base) MAPSORT of: pFN21AB3278.seq Check: 4766 from: 1 to: 7432
pFN21AB3278 7432 bp
With 182 enzymes: SgfI *
October 14, 2009 13:44 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5242 278
Size: 53 83 186 936 3706 2468
Fragments arranged by size:
3706 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2265 2475 2697 4775 1937
Size: 328 210 222 2078 4594
Fragments arranged by size:
4594 2078 328 222 210
Acc65I G'GTAC_C
Cuts at: 3177 4753 3177
Size: 1576 5856
AclI AA'CG_TT
Cuts at: 1563 5559 5932 1563
Size: 3996 373 3063
Fragments arranged by size:
3996 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3218 3736 5495 6879 7147 1632
Size: 1586 518 1759 1384 268 1917
Fragments arranged by size:
1917 1759 1586 1384 518 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7235
AflIII A'CryG_T
Cuts at: 1203 2739 3327 4572 6331 7308 1203
Size: 1536 588 1245 1759 977 1327
Fragments arranged by size:
1759 1536 1327 1245 977 588
AgeI A'CCGG_T
Cuts at: 6263 6263
Size: 7432
AhdI GACnn_n'nnGTC
Cuts at: 6161 6161
Size: 7432
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7432
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2372 3104 3404 4304 4545 1184
Size: 1188 732 300 900 241 4071
Fragments arranged by size:
4071 1188 900 732 300 241
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3029 3061 3029
Size: 32 7400
AlwNI CAG_nnn'CTG
Cuts at: 3616 4305 5175 6747 3616
Size: 689 870 1572 4301
Fragments arranged by size:
4301 1572 870 689
ApaI G_GGCC'C
Cuts at: 3321 4633 3321
Size: 1312 6120
ApaLI G'TGCA_C
Cuts at: 2879 3431 3713 5489 6645 2879
Size: 552 282 1776 1156 3666
Fragments arranged by size:
3666 1776 1156 552 282
ApoI r'AATT_y
Cuts at: 1493 4741 4967 5119 7228 1493
Size: 3248 226 152 2109 1697
Fragments arranged by size:
3248 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5986 160
Size: 872 4954 1606
Fragments arranged by size:
4954 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7432
AvaI C'yCGr_G
Cuts at: 1870 1943 2652 2767 2804 2891 3171 4270 4757
Size: 73 709 115 37 87 280 1099 487
Cuts at: 4757 1870
Size: 4545
Fragments arranged by size:
4545 1099 709 487 280 115 87 73
37
BamHI G'GATC_C
Cuts at: 4762 5129 4762
Size: 367 7065
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3110 3177 3355 3547 3869
Size: 325 217 627 1323 67 178 192 322
Cuts at: 3869 4086 4621 4753 6208 618
Size: 217 535 132 1455 1842
Fragments arranged by size:
1842 1455 1323 627 535 325 322 217
217 192 178 132 67
BanII G_rGCy'C
Cuts at: 1391 2732 2889 3225 3321 4067 4633 4751 1391
Size: 1341 157 336 96 746 566 118 4072
Fragments arranged by size:
4072 1341 746 566 336 157 118 96
BbeI G_GCGC'C
Cuts at: 3551 3873 4090 3551
Size: 322 217 6893
Fragments arranged by size:
6893 322 217
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2957 2976 4368 4444 928
Size: 971 1058 19 1392 76 3916
Fragments arranged by size:
3916 1392 1058 971 76 19
BbvCI CC'TCA_GC
Cuts at: 4019 4019
Size: 7432
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2666 2700 5623 5657 1903
Size: 34 729 34 2923 34 3678
Fragments arranged by size:
3678 2923 729 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5324 6534 5324
Size: 1210 6222
BclI T'GATC_A
Cuts at: 1548 3927 1548
Size: 2379 5053
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3762 3956 4160
Size: 107 122 71 631 605 2090 194 204
Cuts at: 4160 6043 136
Size: 1883 1525
Fragments arranged by size:
2090 1883 1525 631 605 204 194 122
107 71
BglII A'GATC_T
Cuts at: 2763 7427 2763
Size: 4664 2768
BlpI GC'TnA_GC
Cuts at: 2102 3521 4100 4849 2102
Size: 1419 579 749 4685
Fragments arranged by size:
4685 1419 749 579
Bme1580I G_kGCm'C
Cuts at: 1790 2883 3321 3435 3717 4626 4633 5493 6649
Size: 1093 438 114 282 909 7 860 1156
Cuts at: 6649 1790
Size: 2573
Fragments arranged by size:
2573 1156 1093 909 860 438 282 114
7
BmgBI CAC'GTC
Cuts at: 1318 3118 3330 4377 1318
Size: 1800 212 1047 4373
Fragments arranged by size:
4373 1800 1047 212
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3287 5163 6121
Size: 416 53 83 399 24 1870 1876 958
Cuts at: 6121 442
Size: 1753
Fragments arranged by size:
1876 1870 1753 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7432
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2239 2564 2576 2666 4232 4272 4722 6092
Size: 1000 325 12 90 1566 40 450 1370
Cuts at: 6092 1239
Size: 2579
Fragments arranged by size:
2579 1566 1370 1000 450 325 90 40
12
Bpu10I CC'TnA_GC
Cuts at: 2408 4019 2408
Size: 1611 5821
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4319 5557 6422 6720 4319
Size: 1238 865 298 5031
Fragments arranged by size:
5031 1238 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6095 882
Size: 617 4596 2219
Fragments arranged by size:
4596 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2740 3718 5223 493
Size: 711 1536 978 1505 2702
Fragments arranged by size:
2702 1536 1505 978 711
BsaBI GATnn'nnATC
Cuts at: 4499 7426 4499
Size: 2927 4505
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2853 3548 3840 3870
Size: 53 83 186 936 1320 695 292 30
Cuts at: 3870 4087 5239 5621 6256 275
Size: 217 1152 382 635 1451
Fragments arranged by size:
1451 1320 1152 936 695 635 382 292
217 186 83 53 30
BsaWI w'CCGG_w
Cuts at: 3039 5865 6263 6537 6684 7095 3039
Size: 2826 398 274 147 411 3376
Fragments arranged by size:
3376 2826 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2237 2267 2552 2582 1178
Size: 30 165 30 834 30 285 30 6028
Fragments arranged by size:
6028 834 285 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2573 2879 3284 3800 3803 3806 3809 4001
Size: 1387 306 405 516 3 3 3 192
Cuts at: 4001 4641 1186
Size: 640 3977
Fragments arranged by size:
3977 1387 640 516 405 306 192 3
3 3
BseYI C'CCAG_C
Cuts at: 2359 4466 6635 7131 2359
Size: 2107 2169 496 2660
Fragments arranged by size:
2660 2169 2107 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3295 1163
Size: 573 1559 5300
Fragments arranged by size:
5300 1559 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3766 3983 4882 5643 6671 1540
Size: 275 186 1765 217 899 761 1028 2301
Fragments arranged by size:
2301 1765 1028 899 761 275 217 186
BsiHKAI G_wGCw'C
Cuts at: 2012 2108 2883 2889 3435 3653 3686 3717 4067
Size: 96 775 6 546 218 33 31 350
Cuts at: 4067 4106 5493 5578 6649 2012
Size: 39 1387 85 1071 2795
Fragments arranged by size:
2795 1387 1071 775 546 350 218 96
85 39 33 31 6
BsmI GAATG_Cn'
Cuts at: 4943 5036 4943
Size: 93 7339
BsmBI CGTCTCn'nnnn_
Cuts at: 2540 4324 2540
Size: 1784 5648
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2012 2108 2732 2883 2889 3225 3321
Size: 399 222 96 624 151 6 336 96
Cuts at: 3321 3435 3653 3686 3717 4067 4106 4626 4633
Size: 114 218 33 31 350 39 520 7
Cuts at: 4633 4751 5493 5578 6649 1391
Size: 118 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 624 520 399 350 336
222 218 151 118 114 96 96 85
39 33 31 7 6
BspEI T'CCGG_A
Cuts at: 7095 7095
Size: 7432
BspHI T'CATG_A
Cuts at: 3063 5321 7051 3063
Size: 2258 1730 3444
Fragments arranged by size:
3444 2258 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3078 3396 3747 4582 4596 4787 844
Size: 2234 318 351 835 14 191 3489
Fragments arranged by size:
3489 2234 835 351 318 191 14
BsrBI CCG'CTC
Cuts at: 1394 1823 2962 3469 4269 5319 1394
Size: 429 1139 507 800 1050 3507
Fragments arranged by size:
3507 1139 1050 800 507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5927 6101 65
Size: 1183 4679 174 1396
Fragments arranged by size:
4679 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2336 3765 3914 3978 4113 6076 6263
Size: 110 526 1429 149 64 135 1963 187
Cuts at: 6263 1700
Size: 2869
Fragments arranged by size:
2869 1963 1429 526 187 149 135 110
64
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7432
BssHII G'CGCG_C
Cuts at: 1925 3571 3573 3890 1925
Size: 1646 2 317 5467
Fragments arranged by size:
5467 1646 317 2
BssSI C'ACGA_G
Cuts at: 2117 2516 2882 4343 5492 6504 2117
Size: 399 366 1461 1149 1012 3045
Fragments arranged by size:
3045 1461 1149 1012 399 366
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7432
BstBI TT'CG_AA
Cuts at: 6236 6236
Size: 7432
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7432
BstXI CCAn_nnnn'nTGG
Cuts at: 2224 4349 2224
Size: 2125 5307
BstYI r'GATC_y
Cuts at: 1972 2763 4485 4762 5129 5514 5531 6983 7069
Size: 791 1722 277 367 385 17 1452 86
Cuts at: 7069 7081 7427 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1722 1452 791 385 367 346 277
86 17 12
BstZ17I GTA'TAC
Cuts at: 2698 2698
Size: 7432
BtgI C'CryG_G
Cuts at: 513 1065 2918 4050 4068 4642 4668 4714 513
Size: 552 1853 1132 18 574 26 46 3231
Fragments arranged by size:
3231 1853 1132 574 552 46 26 18
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2726 487
Size: 683 56 1500 5193
Fragments arranged by size:
5193 1500 683 56
BtsI GCAGTG_nn'
Cuts at: 4733 4948 5742 5762 4733
Size: 215 794 20 6403
Fragments arranged by size:
6403 794 215 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7432
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7397
DraI TTT'AAA
Cuts at: 4736 4736
Size: 7432
DraIII CAC_nnn'GTG
Cuts at: 3355 3439 3690 3721 3355
Size: 84 251 31 7066
Fragments arranged by size:
7066 251 84 31
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6439 809
Size: 507 5123 1802
Fragments arranged by size:
5123 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3146 3723 3763
Size: 54 1425 50 275 18 1316 577 40
Cuts at: 3763 3819 3980 4443 4479 4639 4717 4879 5768
Size: 56 161 463 36 160 78 162 889
Cuts at: 5768 7397 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1316 889 577 463 275 162
161 160 78 56 54 50 43 40
36 18
EagI C'GGCC_G
Cuts at: 1537 1812 3763 3980 4879 1537
Size: 275 1951 217 899 4090
Fragments arranged by size:
4090 1951 899 275 217
EarI CTCTTCn'nnn_
Cuts at: 1353 2969 5362 1353
Size: 1616 2393 3423
Fragments arranged by size:
3423 2393 1616
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3168 4163 4594 6019 6393 6539
Size: 1064 138 1579 995 431 1425 374 146
Cuts at: 6539 387
Size: 1280
Fragments arranged by size:
1579 1425 1280 1064 995 431 374 146
138
EcoICRI GAG'CTC
Cuts at: 2887 4065 2887
Size: 1178 6254
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2239 2564 2576 2666 3218 3736 4232
Size: 393 607 325 12 90 552 518 496
Cuts at: 4232 4272 4722 5495 6092 6879 7147 1239
Size: 40 450 773 597 787 268 1524
Fragments arranged by size:
1524 787 773 607 597 552 518 496
450 393 325 268 90 40 12
EcoNI CCTnn'n_nnAGG
Cuts at: 2894 2894
Size: 7432
EcoO109I rG'GnC_Cy
Cuts at: 2245 3613 4629 2245
Size: 1368 1016 5048
Fragments arranged by size:
5048 1368 1016
EcoRI G'AATT_C
Cuts at: 4741 5119 4741
Size: 378 7054
EcoRV GAT'ATC
Cuts at: 4075 4075
Size: 7432
FspI TGC'GCA
Cuts at: 2420 4292 5938 2420
Size: 1872 1646 3914
Fragments arranged by size:
3914 1872 1646
HaeII r_GCGC'y
Cuts at: 1678 3551 3873 4090 6579 1678
Size: 1873 322 217 2489 2531
Fragments arranged by size:
2531 2489 1873 322 217
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2237 2269 2552 2584 6073
Size: 32 135 32 665 32 283 32 3489
Cuts at: 6073 6105 6147 6179 1373
Size: 32 42 32 2626
Fragments arranged by size:
3489 2626 665 283 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2086 3283 4570 4776 5022 669
Size: 988 281 148 1197 1287 206 246 3079
Fragments arranged by size:
3079 1287 1197 988 281 246 206 148
HindIII A'AGCT_T
Cuts at: 748 3880 748
Size: 3132 4300
HpaI GTT'AAC
Cuts at: 4570 5022 4570
Size: 452 6980
KasI G'GCGC_C
Cuts at: 3547 3869 4086 3547
Size: 322 217 6893
Fragments arranged by size:
6893 322 217
KpnI G_GTAC'C
Cuts at: 3181 4757 3181
Size: 1576 5856
MfeI C'AATT_G
Cuts at: 5031 5031
Size: 7432
MluI A'CGCG_T
Cuts at: 7308 7308
Size: 7432
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6546 6730 1305
Size: 5241 184 2007
Fragments arranged by size:
5241 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3148 4641 4719 10
Size: 54 1425 343 1316 1493 78 2723
Fragments arranged by size:
2723 1493 1425 1316 343 78 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2222 2354 2375 3542 4286 4347 4527
Size: 648 1056 132 21 1167 744 61 180
Cuts at: 4527 5390 5749 5908 518
Size: 863 359 159 2042
Fragments arranged by size:
2042 1167 1056 863 744 648 359 180
159 132 61 21
MspA1I CmG'CkG
Cuts at: 1606 2481 2920 3525 4552 4848 5525 6673 6918
Size: 875 439 605 1027 296 677 1148 245
Cuts at: 6918 1606
Size: 2120
Fragments arranged by size:
2120 1148 1027 875 677 605 439 296
245
NaeI GCC'GGC
Cuts at: 2338 3916 2338
Size: 1578 5854
NarI GG'CG_CC
Cuts at: 3548 3870 4087 3548
Size: 322 217 6893
Fragments arranged by size:
6893 322 217
NcoI C'CATG_G
Cuts at: 513 1065 4714 513
Size: 552 3649 3231
Fragments arranged by size:
3649 3231 552
NdeI CA'TA_TG
Cuts at: 387 4119 387
Size: 3732 3700
NgoMIV G'CCGG_C
Cuts at: 2336 3914 2336
Size: 1578 5854
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7432
NotI GC'GGCC_GC
Cuts at: 4879 4879
Size: 7432
NspI r_CATG'y
Cuts at: 1132 1229 2380 3264 4187 4291 4790 6335 1132
Size: 97 1151 884 923 104 499 1545 2229
Fragments arranged by size:
2229 1545 1151 923 884 499 104 97
PasI CC'CwG_GG
Cuts at: 1348 2600 4626 1348
Size: 1252 2026 4154
Fragments arranged by size:
4154 2026 1252
PciI A'CATG_T
Cuts at: 6331 6331
Size: 7432
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2454 2946 1108
Size: 1346 492 5594
Fragments arranged by size:
5594 1346 492
PfoI T'CCnGG_A
Cuts at: 2168 2168
Size: 7432
PmeI GTTT'AAAC
Cuts at: 4736 4736
Size: 7432
PmlI CAC'GTG
Cuts at: 3718 3718
Size: 7432
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2111 2143 5501 5533 7014 7046 2111
Size: 32 3358 32 1481 32 2497
Fragments arranged by size:
3358 2497 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2245 2245
Size: 7432
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7432
PsiI TTA'TAA
Cuts at: 5002 5002
Size: 7432
PspOMI G'GGCC_C
Cuts at: 3317 4629 3317
Size: 1312 6120
PspXI vC'TCGA_Gb
Cuts at: 3171 3171
Size: 7432
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3407 3439 3407
Size: 32 7400
PstI C_TGCA'G
Cuts at: 830 2592 2814 3075 4593 4784 830
Size: 1762 222 261 1518 191 3478
Fragments arranged by size:
3478 1762 1518 261 222 191
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7432
PvuII CAG'CTG
Cuts at: 2481 4552 2481
Size: 2071 5361
SacI G_AGCT'C
Cuts at: 2889 4067 2889
Size: 1178 6254
SacII CC_GC'GG
Cuts at: 2921 2921
Size: 7432
SalI G'TCGA_C
Cuts at: 1936 4774 1936
Size: 2838 4594
SapI GCTCTTCn'nnn_
Cuts at: 2969 2969
Size: 7432
SbfI CC_TGCA'GG
Cuts at: 4593 4784 4593
Size: 191 7241
ScaI AGT'ACT
Cuts at: 5680 7392 5680
Size: 1712 5720
SexAI A'CCwGG_T
Cuts at: 3435 4239 3435
Size: 804 6628
SfcI C'TryA_G
Cuts at: 826 1045 2476 2588 2810 3055 3071 4589 4780
Size: 219 1431 112 222 245 16 1518 191
Cuts at: 4780 5915 6596 6787 826
Size: 1135 681 191 1471
Fragments arranged by size:
1518 1471 1431 1135 681 245 222 219
191 191 112 16
SfiI GGCCn_nnn'nGGCC
Cuts at: 4160 4160
Size: 7432
SfoI GGC'GCC
Cuts at: 3549 3871 4088 3549
Size: 322 217 6893
Fragments arranged by size:
6893 322 217
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7432
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7432
SmaI CCC'GGG
Cuts at: 1872 2654 4759 1872
Size: 782 2105 4545
Fragments arranged by size:
4545 2105 782
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2891 3171 4334 5536 6437 6699
Size: 197 926 948 280 1163 1202 901 262
Cuts at: 6699 820
Size: 1553
Fragments arranged by size:
1553 1202 1163 948 926 901 280 262
197
SnaBI TAC'GTA
Cuts at: 493 5223 493
Size: 4730 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7432
SphI G_CATG'C
Cuts at: 1132 4187 4291 4790 1132
Size: 3055 104 499 3774
Fragments arranged by size:
3774 3055 499 104
SspI AAT'ATT
Cuts at: 5 52 5356 5
Size: 47 5304 2081
Fragments arranged by size:
5304 2081 47
StuI AGG'CCT
Cuts at: 1517 4223 4617 1517
Size: 2706 394 4332
Fragments arranged by size:
4332 2706 394
StyI C'CwwG_G
Cuts at: 513 1065 2571 4714 4871 513
Size: 552 1506 2143 157 3074
Fragments arranged by size:
3074 2143 1506 552 157
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3128 5459 5476 5629 5814 6259 325
Size: 1255 1548 2331 17 153 185 445 1498
Fragments arranged by size:
2331 1548 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3579 5678 7390
Size: 274 80 33 51 1443 1602 2099 1712
Cuts at: 7390 96
Size: 138
Fragments arranged by size:
2099 1712 1602 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4361 4393 7392 7424 4361
Size: 32 2999 32 4369
Fragments arranged by size:
4369 2999 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3293 3811 1316
Size: 1977 518 4937
Fragments arranged by size:
4937 1977 518
XbaI T'CTAG_A
Cuts at: 4768 4768
Size: 7432
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3931 3931
Size: 7432
XhoI C'TCGA_G
Cuts at: 1943 2891 3171 1943
Size: 948 280 6204
Fragments arranged by size:
6204 948 280
XmaI C'CCGG_G
Cuts at: 1870 2652 4757 1870
Size: 782 2105 4545
Fragments arranged by size:
4545 2105 782
XmnI GAAnn'nnTTC
Cuts at: 4352 5561 4352
Size: 1209 6223
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5240 276
Size: 53 83 186 936 3706 2468
Fragments arranged by size:
3706 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI
PstI PvuI PvuII SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BfrBI BplI BsiWI
Bsu36I FalI FseI FspAI NruI NsiI PacI RsrII
SanDI SrfI SwaI