Restriction map for
pFN21AB3253
(Circular) (Six-base) MAPSORT of: pFN21AB3253.seq Check: 6308 from: 1 to: 6568
pFN21AB3253 6568 bp
With 182 enzymes: SgfI *
February 27, 2009 14:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4378 278
Size: 53 83 186 936 2842 2468
Fragments arranged by size:
2842 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3911 1937
Size: 1974 4594
Acc65I G'GTAC_C
Cuts at: 3889 3889
Size: 6568
AclI AA'CG_TT
Cuts at: 1563 4695 5068 1563
Size: 3132 373 3063
Fragments arranged by size:
3132 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2212 2831 3257 4631 6015 6283 1632
Size: 580 619 426 1374 1384 268 1917
Fragments arranged by size:
1917 1384 1374 619 580 426 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6371
AflIII A'CryG_T
Cuts at: 1203 3495 5467 6444 1203
Size: 2292 1972 977 1327
Fragments arranged by size:
2292 1972 1327 977
AgeI A'CCGG_T
Cuts at: 5399 5399
Size: 6568
AhdI GACnn_n'nnGTC
Cuts at: 5297 5297
Size: 6568
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6568
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6568
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3508 3540 3508
Size: 32 6536
AlwNI CAG_nnn'CTG
Cuts at: 3727 3824 4311 5883 3727
Size: 97 487 1572 4412
Fragments arranged by size:
4412 1572 487 97
ApaLI G'TGCA_C
Cuts at: 4625 5781 4625
Size: 1156 5412
ApoI r'AATT_y
Cuts at: 1493 3137 3251 3877 4103 4255 6364 1493
Size: 1644 114 626 226 152 2109 1697
Fragments arranged by size:
2109 1697 1644 626 226 152 114
AseI AT'TA_AT
Cuts at: 160 1032 5122 160
Size: 872 4090 1606
Fragments arranged by size:
4090 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6568
AvaI C'yCGr_G
Cuts at: 1870 1943 2917 3893 1870
Size: 73 974 976 4545
Fragments arranged by size:
4545 976 974 73
AvrII C'CTAG_G
Cuts at: 3527 3527
Size: 6568
BamHI G'GATC_C
Cuts at: 3898 4265 3898
Size: 367 6201
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2643 3889 5344 618
Size: 325 217 627 856 1246 1455 1842
Fragments arranged by size:
1842 1455 1246 856 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2918 3348 3887 1391
Size: 1527 430 539 4072
Fragments arranged by size:
4072 1527 539 430
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2784 2953 3368 3399 928
Size: 971 885 169 415 31 4097
Fragments arranged by size:
4097 971 885 415 169 31
BbvCI CC'TCA_GC
Cuts at: 2397 2397
Size: 6568
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4759 4793 1903
Size: 34 2822 34 3678
Fragments arranged by size:
3678 2822 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2710 4460 5670 2710
Size: 1750 1210 3608
Fragments arranged by size:
3608 1750 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6568
BfrBI ATG'CAT
Cuts at: 2330 2330
Size: 6568
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5179 136
Size: 107 122 71 631 605 3507 1525
Fragments arranged by size:
3507 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2320 6563 2320
Size: 4243 2325
BlpI GC'TnA_GC
Cuts at: 3985 3985
Size: 6568
Bme1580I G_kGCm'C
Cuts at: 1790 4629 5785 1790
Size: 2839 1156 2573
Fragments arranged by size:
2839 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6568
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2558 4299 5257
Size: 416 53 83 399 24 1141 1741 958
Cuts at: 5257 442
Size: 1753
Fragments arranged by size:
1753 1741 1141 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6568
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3362 3362
Size: 6568
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2669 5228 1239
Size: 1430 2559 2579
Fragments arranged by size:
2579 2559 1430
Bpu10I CC'TnA_GC
Cuts at: 2397 2397
Size: 6568
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3332 4693 5558 5856 3332
Size: 1361 865 298 4044
Fragments arranged by size:
4044 1361 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5231 882
Size: 617 3732 2219
Fragments arranged by size:
3732 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4359 493
Size: 711 3155 2702
Fragments arranged by size:
3155 2702 711
BsaBI GATnn'nnATC
Cuts at: 6562 6562
Size: 6568
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4375 4757 5392 275
Size: 53 83 186 936 2842 382 635 1451
Fragments arranged by size:
2842 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2016 2544 2903 5001 5399 5673 5820 6231 2016
Size: 528 359 2098 398 274 147 411 2353
Fragments arranged by size:
2353 2098 528 411 398 359 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6343
Fragments arranged by size:
6343 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6568
BseYI C'CCAG_C
Cuts at: 5771 6267 5771
Size: 496 6072
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5995
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4018 4779 5807 1540
Size: 275 186 2017 761 1028 2301
Fragments arranged by size:
2301 2017 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2918 3348 4629 4714 5785 2918
Size: 430 1281 85 1071 3701
Fragments arranged by size:
3701 1281 1071 430 85
BsmI GAATG_Cn'
Cuts at: 2740 4079 4172 2740
Size: 1339 93 5136
Fragments arranged by size:
5136 1339 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2918 3348 3887 4629 4714 5785 1391
Size: 399 1128 430 539 742 85 1071 2174
Fragments arranged by size:
2174 1128 1071 742 539 430 399 85
BspEI T'CCGG_A
Cuts at: 2016 2544 6231 2016
Size: 528 3687 2353
Fragments arranged by size:
3687 2353 528
BspHI T'CATG_A
Cuts at: 2832 4457 6187 2832
Size: 1625 1730 3213
Fragments arranged by size:
3213 1730 1625
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3832 3923 844
Size: 2988 91 3489
Fragments arranged by size:
3489 2988 91
BsrBI CCG'CTC
Cuts at: 1394 1823 4455 1394
Size: 429 2632 3507
Fragments arranged by size:
3507 2632 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2745 5063 5237 65
Size: 1183 1497 2318 174 1396
Fragments arranged by size:
2318 1497 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5212 5399 1700
Size: 110 3402 187 2869
Fragments arranged by size:
3402 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6568
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6568
BssSI C'ACGA_G
Cuts at: 4628 5640 4628
Size: 1012 5556
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3727 794
Size: 2933 3635
BstBI TT'CG_AA
Cuts at: 3751 5372 3751
Size: 1621 4947
BstEII G'GTnAC_C
Cuts at: 1184 3167 1184
Size: 1983 4585
BstXI CCAn_nnnn'nTGG
Cuts at: 2647 3164 2647
Size: 517 6051
BstYI r'GATC_y
Cuts at: 1972 2320 3120 3276 3898 4265 4650 4667 6119
Size: 348 800 156 622 367 385 17 1452
Cuts at: 6119 6205 6217 6563 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 800 622 385 367 348 346
156 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5077
Fragments arranged by size:
5077 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5829
Fragments arranged by size:
5829 683 56
BtsI GCAGTG_nn'
Cuts at: 3518 4084 4878 4898 3518
Size: 566 794 20 5188
Fragments arranged by size:
5188 794 566 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6568
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6533
DraI TTT'AAA
Cuts at: 3715 3872 3715
Size: 157 6411
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5575 809
Size: 507 4259 1802
Fragments arranged by size:
4259 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2742 2967 3154
Size: 54 1425 50 275 18 912 225 187
Cuts at: 3154 3377 4015 4904 6533 8
Size: 223 638 889 1629 43
Fragments arranged by size:
1629 1425 912 889 638 275 225 223
187 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4015 1537
Size: 275 2203 4090
Fragments arranged by size:
4090 2203 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3336 3753 3798 4498 1353
Size: 1983 417 45 700 3423
Fragments arranged by size:
3423 1983 700 417 45
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5155 5529 5675 387
Size: 1064 138 3566 374 146 1280
Fragments arranged by size:
3566 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2916 3346 2916
Size: 430 6138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2212 2669 2831 3257 4631 5228 6015
Size: 393 580 457 162 426 1374 597 787
Cuts at: 6015 6283 1239
Size: 268 1524
Fragments arranged by size:
1524 1374 787 597 580 457 426 393
268 162
EcoNI CCTnn'n_nnAGG
Cuts at: 3076 3653 3076
Size: 577 5991
EcoRI G'AATT_C
Cuts at: 3251 3877 4255 3251
Size: 626 378 5564
Fragments arranged by size:
5564 626 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3284 3424 3879 3284
Size: 140 455 5973
Fragments arranged by size:
5973 455 140
FspI TGC'GCA
Cuts at: 5074 5074
Size: 6568
HaeII r_GCGC'y
Cuts at: 1678 5715 1678
Size: 4037 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2885 2917 3107 3139 3590
Size: 32 135 32 1313 32 190 32 451
Cuts at: 3590 3622 5209 5241 5283 5315 1373
Size: 32 1587 32 42 32 2626
Fragments arranged by size:
2626 1587 1313 451 190 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3912 4158 669
Size: 988 281 1974 246 3079
Fragments arranged by size:
3079 1974 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6568
HpaI GTT'AAC
Cuts at: 4158 4158
Size: 6568
KpnI G_GTAC'C
Cuts at: 3893 3893
Size: 6568
MfeI C'AATT_G
Cuts at: 4167 4167
Size: 6568
MluI A'CGCG_T
Cuts at: 6444 6444
Size: 6568
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2791 3141 5682 5866 1305
Size: 1486 350 2541 184 2007
Fragments arranged by size:
2541 2007 1486 350 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2744 2969 3156 3379 10
Size: 54 1425 343 912 225 187 223 3199
Fragments arranged by size:
3199 1425 912 343 225 223 187 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4526 4885 5044 518
Size: 648 3360 359 159 2042
Fragments arranged by size:
3360 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2756 3984 4661 5809 6054 1606
Size: 1150 1228 677 1148 245 2120
Fragments arranged by size:
2120 1228 1150 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5077
Fragments arranged by size:
5077 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6568
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6568
NotI GC'GGCC_GC
Cuts at: 4015 4015
Size: 6568
NsiI A_TGCA'T
Cuts at: 2332 2332
Size: 6568
NspI r_CATG'y
Cuts at: 1132 1229 3499 3926 5471 1132
Size: 97 2270 427 1545 2229
Fragments arranged by size:
2270 2229 1545 427 97
PasI CC'CwG_GG
Cuts at: 1348 3049 1348
Size: 1701 4867
PciI A'CATG_T
Cuts at: 3495 5467 3495
Size: 1972 4596
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2627 1108
Size: 1519 5049
PmeI GTTT'AAAC
Cuts at: 3872 3872
Size: 6568
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4637 4669 6150 6182 4637
Size: 32 1481 32 5023
Fragments arranged by size:
5023 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2027 1613
Size: 414 6154
PsiI TTA'TAA
Cuts at: 2340 4138 2340
Size: 1798 4770
PstI C_TGCA'G
Cuts at: 830 2959 3920 830
Size: 2129 961 3478
Fragments arranged by size:
3478 2129 961
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6568
PvuII CAG'CTG
Cuts at: 2756 2756
Size: 6568
SacI G_AGCT'C
Cuts at: 2918 3348 2918
Size: 430 6138
SalI G'TCGA_C
Cuts at: 1936 3910 1936
Size: 1974 4594
SapI GCTCTTCn'nnn_
Cuts at: 3336 3753 3336
Size: 417 6151
SbfI CC_TGCA'GG
Cuts at: 3920 3920
Size: 6568
ScaI AGT'ACT
Cuts at: 3407 4816 6528 3407
Size: 1409 1712 3447
Fragments arranged by size:
3447 1712 1409
SfcI C'TryA_G
Cuts at: 826 1045 2955 3076 3421 3756 3916 5051 5732
Size: 219 1910 121 345 335 160 1135 681
Cuts at: 5732 5923 826
Size: 191 1471
Fragments arranged by size:
1910 1471 1135 681 345 335 219 191
160 121
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6568
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6568
SmaI CCC'GGG
Cuts at: 1872 3895 1872
Size: 2023 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3347 4672 5573 5835 820
Size: 197 926 1404 1325 901 262 1553
Fragments arranged by size:
1553 1404 1325 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4359 493
Size: 3866 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6568
SphI G_CATG'C
Cuts at: 1132 3926 1132
Size: 2794 3774
SspI AAT'ATT
Cuts at: 5 52 2794 2824 4492 5
Size: 47 2742 30 1668 2081
Fragments arranged by size:
2742 2081 1668 47 30
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6568
StyI C'CwwG_G
Cuts at: 513 1065 2004 2025 3316 3527 3654 4007 513
Size: 552 939 21 1291 211 127 353 3074
Fragments arranged by size:
3074 1291 939 552 353 211 127 21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2067 3242 4595 4612 4765 4950 5395
Size: 1255 487 1175 1353 17 153 185 445
Cuts at: 5395 325
Size: 1498
Fragments arranged by size:
1498 1353 1255 1175 487 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3405 4814 6526
Size: 274 80 33 51 1443 1428 1409 1712
Cuts at: 6526 96
Size: 138
Fragments arranged by size:
1712 1443 1428 1409 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3200 3232 6528 6560 3200
Size: 32 3296 32 3208
Fragments arranged by size:
3296 3208 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6568
XbaI T'CTAG_A
Cuts at: 3904 3904
Size: 6568
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2430 2430
Size: 6568
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6568
XmaI C'CCGG_G
Cuts at: 1870 3893 1870
Size: 2023 4545
XmnI GAAnn'nnTTC
Cuts at: 2823 3234 3264 4697 2823
Size: 411 30 1433 4694
Fragments arranged by size:
4694 1433 411 30
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4376 276
Size: 53 83 186 936 2842 2468
Fragments arranged by size:
2842 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI
SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI BaeI BbeI BsiWI BsmBI
BstZ17I Bsu36I DraIII EcoO109I EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI
PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI
SfoI SrfI SwaI