Restriction map for
pFN21AB3247
(Circular) (Six-base) MAPSORT of: pFN21AB3247.seq Check: 3887 from: 1 to: 5788
pFN21AB3247 5788 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2087 2087
Size: 5788
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3598 278
Size: 53 83 186 936 2062 2468
Fragments arranged by size:
2468 2062 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3131 1937
Size: 1194 4594
Acc65I G'GTAC_C
Cuts at: 3109 3109
Size: 5788
AclI AA'CG_TT
Cuts at: 1563 3915 4288 1563
Size: 2352 373 3063
Fragments arranged by size:
3063 2352 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2352 2400 2520 2556 3851 5235 5503 1632
Size: 720 48 120 36 1295 1384 268 1917
Fragments arranged by size:
1917 1384 1295 720 268 120 48 36
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5591
AflIII A'CryG_T
Cuts at: 1203 4687 5664 1203
Size: 3484 977 1327
Fragments arranged by size:
3484 1327 977
AgeI A'CCGG_T
Cuts at: 4619 4619
Size: 5788
AhdI GACnn_n'nnGTC
Cuts at: 4517 4517
Size: 5788
AleI CACnn'nnGTG
Cuts at: 1166 2312 2694 1166
Size: 1146 382 4260
Fragments arranged by size:
4260 1146 382
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5788
AlwNI CAG_nnn'CTG
Cuts at: 2634 3531 5103 2634
Size: 897 1572 3319
Fragments arranged by size:
3319 1572 897
ApaLI G'TGCA_C
Cuts at: 3845 5001 3845
Size: 1156 4632
ApoI r'AATT_y
Cuts at: 1493 2799 3097 3323 3475 5584 1493
Size: 1306 298 226 152 2109 1697
Fragments arranged by size:
2109 1697 1306 298 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4342 160
Size: 872 3310 1606
Fragments arranged by size:
3310 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5788
AvaI C'yCGr_G
Cuts at: 1870 1943 3113 1870
Size: 73 1170 4545
Fragments arranged by size:
4545 1170 73
BamHI G'GATC_C
Cuts at: 2274 3118 3485 2274
Size: 844 367 4577
Fragments arranged by size:
4577 844 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2459 3109 4564 618
Size: 325 217 627 672 650 1455 1842
Fragments arranged by size:
1842 1455 672 650 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3107 1391
Size: 1716 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2508 2631 928
Size: 971 609 123 4085
Fragments arranged by size:
4085 971 609 123
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3979 4013 1903
Size: 34 2042 34 3678
Fragments arranged by size:
3678 2042 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3680 4890 3680
Size: 1210 4578
BclI T'GATC_A
Cuts at: 1548 2814 2910 1548
Size: 1266 96 4426
Fragments arranged by size:
4426 1266 96
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4399 136
Size: 107 122 71 631 605 2727 1525
Fragments arranged by size:
2727 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5783 5783
Size: 5788
BlpI GC'TnA_GC
Cuts at: 2054 3205 2054
Size: 1151 4637
Bme1580I G_kGCm'C
Cuts at: 1790 2259 3849 5005 1790
Size: 469 1590 1156 2573
Fragments arranged by size:
2573 1590 1156 469
BmgBI CAC'GTC
Cuts at: 1318 2536 1318
Size: 1218 4570
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2335 2688 3519
Size: 416 53 83 399 24 918 353 831
Cuts at: 3519 4477 442
Size: 958 1753
Fragments arranged by size:
1753 958 918 831 416 399 353 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5788
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2536 3006 4448 1239
Size: 1297 470 1442 2579
Fragments arranged by size:
2579 1442 1297 470
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3913 4778 5076 3913
Size: 865 298 4625
Fragments arranged by size:
4625 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2222 2667 4451 882
Size: 617 723 445 1784 2219
Fragments arranged by size:
2219 1784 723 617 445
BsaAI yAC'GTr
Cuts at: 493 1204 2110 2260 3579 493
Size: 711 906 150 1319 2702
Fragments arranged by size:
2702 1319 906 711 150
BsaBI GATnn'nnATC
Cuts at: 2725 5782 2725
Size: 3057 2731
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3595 3977 4612 275
Size: 53 83 186 936 2062 382 635 1451
Fragments arranged by size:
2062 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4221 4619 4893 5040 5451 4221
Size: 398 274 147 411 4558
Fragments arranged by size:
4558 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2270 2300 1178
Size: 30 165 30 867 30 4666
Fragments arranged by size:
4666 867 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2539 1186
Size: 1353 4435
BseYI C'CCAG_C
Cuts at: 2513 4991 5487 2513
Size: 2478 496 2814
Fragments arranged by size:
2814 2478 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2316 1163
Size: 573 580 4635
Fragments arranged by size:
4635 580 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3238 3999 5027 1540
Size: 275 186 1237 761 1028 2301
Fragments arranged by size:
2301 1237 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3849 3934 5005 3849
Size: 85 1071 4632
Fragments arranged by size:
4632 1071 85
BsmI GAATG_Cn'
Cuts at: 3299 3392 3299
Size: 93 5695
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2259 3107 3849 3934 5005 1391
Size: 399 469 848 742 85 1071 2174
Fragments arranged by size:
2174 1071 848 742 469 399 85
BspEI T'CCGG_A
Cuts at: 5451 5451
Size: 5788
BspHI T'CATG_A
Cuts at: 3677 5407 3677
Size: 1730 4058
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2087 2093 2226 2240 2415 3066 3143 844
Size: 1243 6 133 14 175 651 77 3489
Fragments arranged by size:
3489 1243 651 175 133 77 14 6
BsrBI CCG'CTC
Cuts at: 1394 1823 3675 1394
Size: 429 1852 3507
Fragments arranged by size:
3507 1852 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2862 2929 4283 4457 65
Size: 1183 1614 67 1354 174 1396
Fragments arranged by size:
1614 1396 1354 1183 174 67
BsrFI r'CCGG_y
Cuts at: 1700 1810 4432 4619 1700
Size: 110 2622 187 2869
Fragments arranged by size:
2869 2622 187 110
BsrGI T'GTAC_A
Cuts at: 96 2316 2390 2605 96
Size: 2220 74 215 3279
Fragments arranged by size:
3279 2220 215 74
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5788
BssSI C'ACGA_G
Cuts at: 2045 3848 4860 2045
Size: 1803 1012 2973
Fragments arranged by size:
2973 1803 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5788
BstBI TT'CG_AA
Cuts at: 4592 4592
Size: 5788
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5788
BstXI CCAn_nnnn'nTGG
Cuts at: 2696 2696
Size: 5788
BstYI r'GATC_y
Cuts at: 1972 2274 2720 3118 3485 3870 3887 5339 5425
Size: 302 446 398 367 385 17 1452 86
Cuts at: 5425 5437 5783 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 446 398 385 367 346 302
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4297
Fragments arranged by size:
4297 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5049
Fragments arranged by size:
5049 683 56
BtsI GCAGTG_nn'
Cuts at: 3304 4098 4118 3304
Size: 794 20 4974
Fragments arranged by size:
4974 794 20
ClaI AT'CG_AT
Cuts at: 1554 2070 1554
Size: 516 5272
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5753
DraI TTT'AAA
Cuts at: 3092 3092
Size: 5788
DraIII CAC_nnn'GTG
Cuts at: 2263 2263
Size: 5788
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4795 809
Size: 507 3479 1802
Fragments arranged by size:
3479 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3235 4124 5753
Size: 54 1425 50 275 18 1405 889 1629
Cuts at: 5753 8
Size: 43
Fragments arranged by size:
1629 1425 1405 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3235 1537
Size: 275 1423 4090
Fragments arranged by size:
4090 1423 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3718 1353
Size: 2365 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4375 4749 4895 387
Size: 1064 138 2786 374 146 1280
Fragments arranged by size:
2786 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2352 2400 2520 2536 2556 3006 3851
Size: 393 720 48 120 16 20 450 845
Cuts at: 3851 4448 5235 5503 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 845 787 720 597 450 393 268
120 48 20 16
EcoNI CCTnn'n_nnAGG
Cuts at: 2162 2162
Size: 5788
EcoO109I rG'GnC_Cy
Cuts at: 2703 2703
Size: 5788
EcoRI G'AATT_C
Cuts at: 3097 3475 3097
Size: 378 5410
FspI TGC'GCA
Cuts at: 4294 4294
Size: 5788
HaeII r_GCGC'y
Cuts at: 1678 4935 1678
Size: 3257 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2028 2060 2378 2410 2568
Size: 32 135 32 456 32 318 32 158
Cuts at: 2568 2600 2723 2755 4429 4461 4503 4535 1373
Size: 32 123 32 1674 32 42 32 2626
Fragments arranged by size:
2626 1674 456 318 158 135 123 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2449 2872 3132 3378 669
Size: 988 281 511 423 260 246 3079
Fragments arranged by size:
3079 988 511 423 281 260 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5788
HpaI GTT'AAC
Cuts at: 2449 3378 2449
Size: 929 4859
KpnI G_GTAC'C
Cuts at: 3113 3113
Size: 5788
MfeI C'AATT_G
Cuts at: 3387 3387
Size: 5788
MluI A'CGCG_T
Cuts at: 5664 5664
Size: 5788
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2355 4902 5086 1305
Size: 1050 2547 184 2007
Fragments arranged by size:
2547 2007 1050 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3966
Fragments arranged by size:
3966 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2312 2694 2742 3746 4105 4264 518
Size: 648 1146 382 48 1004 359 159 2042
Fragments arranged by size:
2042 1146 1004 648 382 359 159 48
MspA1I CmG'CkG
Cuts at: 1606 2053 2153 2544 3041 3204 3881 5029 5274
Size: 447 100 391 497 163 677 1148 245
Cuts at: 5274 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 497 447 391 245 163
100
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4297
Fragments arranged by size:
4297 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5788
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5788
NotI GC'GGCC_GC
Cuts at: 3235 3235
Size: 5788
NspI r_CATG'y
Cuts at: 1132 1229 3146 4691 1132
Size: 97 1917 1545 2229
Fragments arranged by size:
2229 1917 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5788
PciI A'CATG_T
Cuts at: 4687 4687
Size: 5788
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5788
PmeI GTTT'AAAC
Cuts at: 3092 3092
Size: 5788
PmlI CAC'GTG
Cuts at: 2110 2260 2110
Size: 150 5638
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3857 3889 5370 5402 3857
Size: 32 1481 32 4243
Fragments arranged by size:
4243 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2703 2703
Size: 5788
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5788
PsiI TTA'TAA
Cuts at: 3358 3358
Size: 5788
PstI C_TGCA'G
Cuts at: 830 2237 3063 3140 830
Size: 1407 826 77 3478
Fragments arranged by size:
3478 1407 826 77
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5788
PvuII CAG'CTG
Cuts at: 3041 3041
Size: 5788
SalI G'TCGA_C
Cuts at: 1936 3130 1936
Size: 1194 4594
SanDI GG'GwC_CC
Cuts at: 2703 2703
Size: 5788
SbfI CC_TGCA'GG
Cuts at: 2237 3140 2237
Size: 903 4885
ScaI AGT'ACT
Cuts at: 4036 5748 4036
Size: 1712 4076
SfcI C'TryA_G
Cuts at: 826 1045 2128 2233 3059 3136 4271 4952 5143
Size: 219 1083 105 826 77 1135 681 191
Cuts at: 5143 826
Size: 1471
Fragments arranged by size:
1471 1135 1083 826 681 219 191 105
77
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5788
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5788
SmaI CCC'GGG
Cuts at: 1872 3115 1872
Size: 1243 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3892 4793 5055 820
Size: 197 926 1949 901 262 1553
Fragments arranged by size:
1949 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3579 493
Size: 3086 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5788
SphI G_CATG'C
Cuts at: 1132 3146 1132
Size: 2014 3774
SspI AAT'ATT
Cuts at: 5 52 3712 5
Size: 47 3660 2081
Fragments arranged by size:
3660 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5788
StyI C'CwwG_G
Cuts at: 513 1065 2004 2838 3227 513
Size: 552 939 834 389 3074
Fragments arranged by size:
3074 939 834 552 389
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3815 3832 3985 4170 4615 325
Size: 1255 2235 17 153 185 445 1498
Fragments arranged by size:
2235 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2316 2390 2605
Size: 274 80 33 51 1443 339 74 215
Cuts at: 2605 4034 5746 96
Size: 1429 1712 138
Fragments arranged by size:
1712 1443 1429 339 274 215 138 80
74 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2270 2302 5748 5780 2270
Size: 32 3446 32 2278
Fragments arranged by size:
3446 2278 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5788
XbaI T'CTAG_A
Cuts at: 3124 3124
Size: 5788
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2714 2714
Size: 5788
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5788
XmaI C'CCGG_G
Cuts at: 1870 3113 1870
Size: 1243 4545
XmnI GAAnn'nnTTC
Cuts at: 3917 3917
Size: 5788
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3596 276
Size: 53 83 186 936 2062 2468
Fragments arranged by size:
2468 2062 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PspOMI PspXI PsrI RsrII
SacI SacII SapI SexAI SfiI SfoI SrfI SwaI