Restriction map for
pFN21AB3246
(Circular) (Six-base) MAPSORT of: pFN21AB3246.seq Check: 7908 from: 1 to: 7831
pFN21AB3246 7831 bp
With 182 enzymes: SgfI *
February 11, 2011 18:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2963 2963
Size: 7831
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3950 5641 278
Size: 53 83 186 936 2414 1691 2468
Fragments arranged by size:
2468 2414 1691 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5174 1937
Size: 3237 4594
Acc65I G'GTAC_C
Cuts at: 5152 5152
Size: 7831
AclI AA'CG_TT
Cuts at: 1563 5958 6331 1563
Size: 4395 373 3063
Fragments arranged by size:
4395 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2154 2735 2949 3576 4058 4154 5894 7278
Size: 522 581 214 627 482 96 1740 1384
Cuts at: 7278 7546 1632
Size: 268 1917
Fragments arranged by size:
1917 1740 1384 627 581 522 482 268
214 96
AflII C'TTAA_G
Cuts at: 820 1017 2099 2360 820
Size: 197 1082 261 6291
Fragments arranged by size:
6291 1082 261 197
AflIII A'CryG_T
Cuts at: 1203 6730 7707 1203
Size: 5527 977 1327
Fragments arranged by size:
5527 1327 977
AgeI A'CCGG_T
Cuts at: 6662 6662
Size: 7831
AhdI GACnn_n'nnGTC
Cuts at: 3478 3946 6560 3478
Size: 468 2614 4749
Fragments arranged by size:
4749 2614 468
AleI CACnn'nnGTG
Cuts at: 1166 3896 4175 4229 1166
Size: 2730 279 54 4768
Fragments arranged by size:
4768 2730 279 54
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3430 1184
Size: 2246 5585
AlwNI CAG_nnn'CTG
Cuts at: 3332 4903 5574 7146 3332
Size: 1571 671 1572 4017
Fragments arranged by size:
4017 1572 1571 671
ApaLI G'TGCA_C
Cuts at: 3574 5888 7044 3574
Size: 2314 1156 4361
Fragments arranged by size:
4361 2314 1156
ApoI r'AATT_y
Cuts at: 1493 2768 3449 4067 5140 5366 5518 7627 1493
Size: 1275 681 618 1073 226 152 2109 1697
Fragments arranged by size:
2109 1697 1275 1073 681 618 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6385 160
Size: 872 5353 1606
Fragments arranged by size:
5353 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7831
AvaI C'yCGr_G
Cuts at: 1870 1943 4032 4352 5156 1870
Size: 73 2089 320 804 4545
Fragments arranged by size:
4545 2089 804 320 73
BamHI G'GATC_C
Cuts at: 3802 5161 5528 3802
Size: 1359 367 6105
Fragments arranged by size:
6105 1359 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 5152 6607 618
Size: 325 217 627 3365 1455 1842
Fragments arranged by size:
3365 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2117 4166 4808 5150 1391
Size: 726 2049 642 342 4072
Fragments arranged by size:
4072 2049 726 642 342
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2184 928
Size: 971 285 6575
Fragments arranged by size:
6575 971 285
BbvCI CC'TCA_GC
Cuts at: 4769 4769
Size: 7831
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3990 4024 4109 4143 6022 6056 1903
Size: 34 2053 34 85 34 1879 34 3678
Fragments arranged by size:
3678 2053 1879 85 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5723 6933 5723
Size: 1210 6621
BclI T'GATC_A
Cuts at: 1548 3870 4485 1548
Size: 2322 615 4894
Fragments arranged by size:
4894 2322 615
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2052 3918 6442
Size: 107 122 71 631 605 380 1866 2524
Cuts at: 6442 136
Size: 1525
Fragments arranged by size:
2524 1866 1525 631 605 380 122 107
71
BglII A'GATC_T
Cuts at: 7826 7826
Size: 7831
BlpI GC'TnA_GC
Cuts at: 2075 5248 2075
Size: 3173 4658
Bme1580I G_kGCm'C
Cuts at: 1790 2838 3578 3893 5892 7048 1790
Size: 1048 740 315 1999 1156 2573
Fragments arranged by size:
2573 1999 1156 1048 740 315
BmgBI CAC'GTC
Cuts at: 1318 3943 1318
Size: 2625 5206
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3885 4430 4891
Size: 416 53 83 399 24 2468 545 461
Cuts at: 4891 5562 6520 442
Size: 671 958 1753
Fragments arranged by size:
2468 1753 958 671 545 461 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7831
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3841 3841
Size: 7831
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2703 3639 6491 1239
Size: 1464 936 2852 2579
Fragments arranged by size:
2852 2579 1464 936
Bpu10I CC'TnA_GC
Cuts at: 4769 4769
Size: 7831
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3755 4937 5956 6821 7119 3755
Size: 1182 1019 865 298 4467
Fragments arranged by size:
4467 1182 1019 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3758 6494 882
Size: 617 2259 2736 2219
Fragments arranged by size:
2736 2259 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3470 5622 493
Size: 711 2266 2152 2702
Fragments arranged by size:
2702 2266 2152 711
BsaBI GATnn'nnATC
Cuts at: 7825 7825
Size: 7831
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2055 3947 4005 5638
Size: 53 83 186 936 522 1892 58 1633
Cuts at: 5638 6020 6655 275
Size: 382 635 1451
Fragments arranged by size:
1892 1633 1451 936 635 522 382 186
83 58 53
BsaWI w'CCGG_w
Cuts at: 6264 6662 6936 7083 7494 6264
Size: 398 274 147 411 6601
Fragments arranged by size:
6601 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2658 2688 4205 4235 1178
Size: 30 165 30 1255 30 1517 30 4774
Fragments arranged by size:
4774 1517 1255 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2048 3817 4259 1186
Size: 862 1769 442 4758
Fragments arranged by size:
4758 1769 862 442
BseYI C'CCAG_C
Cuts at: 2041 2651 3996 7034 7530 2041
Size: 610 1345 3038 496 2342
Fragments arranged by size:
3038 2342 1345 610 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2435 4401 5018 1163
Size: 573 699 1966 617 3976
Fragments arranged by size:
3976 1966 699 617 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5281 6042 7070 1540
Size: 275 186 3280 761 1028 2301
Fragments arranged by size:
3280 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3259 3553 3578 5892 5977 7048 3259
Size: 294 25 2314 85 1071 4042
Fragments arranged by size:
4042 2314 1071 294 85 25
BsmI GAATG_Cn'
Cuts at: 5342 5435 5342
Size: 93 7738
BsmBI CGTCTCn'nnnn_
Cuts at: 3996 4940 3996
Size: 944 6887
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2117 2838 3259 3553 3578 3893 4166
Size: 399 327 721 421 294 25 315 273
Cuts at: 4166 4808 5150 5892 5977 7048 1391
Size: 642 342 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 721 642 421 399 342
327 315 294 273 85 25
BspEI T'CCGG_A
Cuts at: 7494 7494
Size: 7831
BspHI T'CATG_A
Cuts at: 3700 3867 4449 5720 7450 3700
Size: 167 582 1271 1730 4081
Fragments arranged by size:
4081 1730 1271 582 167
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2963 5186 844
Size: 2119 2223 3489
Fragments arranged by size:
3489 2223 2119
BsrBI CCG'CTC
Cuts at: 1394 1823 5718 1394
Size: 429 3895 3507
Fragments arranged by size:
3895 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2643 2808 4152 4569 6326 6500 65
Size: 1183 1395 165 1344 417 1757 174 1396
Fragments arranged by size:
1757 1396 1395 1344 1183 417 174 165
BsrFI r'CCGG_y
Cuts at: 1700 1810 2128 3843 6475 6662 1700
Size: 110 318 1715 2632 187 2869
Fragments arranged by size:
2869 2632 1715 318 187 110
BsrGI T'GTAC_A
Cuts at: 96 3497 96
Size: 3401 4430
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7831
BssSI C'ACGA_G
Cuts at: 2595 4799 5891 6903 2595
Size: 2204 1092 1012 3523
Fragments arranged by size:
3523 2204 1092 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7831
BstBI TT'CG_AA
Cuts at: 3004 6635 3004
Size: 3631 4200
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7831
BstXI CCAn_nnnn'nTGG
Cuts at: 2029 2959 3227 4231 4420 2029
Size: 930 268 1004 189 5440
Fragments arranged by size:
5440 1004 930 268 189
BstYI r'GATC_y
Cuts at: 1972 2706 3802 5161 5528 5913 5930 7382 7468
Size: 734 1096 1359 367 385 17 1452 86
Cuts at: 7468 7480 7826 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1359 1096 734 385 367 346
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 4545 513
Size: 552 939 2541 3799
Fragments arranged by size:
3799 2541 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3694 4122 487
Size: 683 56 2468 428 4196
Fragments arranged by size:
4196 2468 683 428 56
BtsI GCAGTG_nn'
Cuts at: 2907 3674 3891 4170 4413 5347 6141 6161 2907
Size: 767 217 279 243 934 794 20 4577
Fragments arranged by size:
4577 934 794 767 279 243 217 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7831
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7796
DraI TTT'AAA
Cuts at: 2893 5135 2893
Size: 2242 5589
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6838 809
Size: 507 5522 1802
Fragments arranged by size:
5522 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3693 3900 5051
Size: 54 1425 50 275 18 1863 207 1151
Cuts at: 5051 5278 6167 7796 8
Size: 227 889 1629 43
Fragments arranged by size:
1863 1629 1425 1151 889 275 227 207
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5278 1537
Size: 275 3466 4090
Fragments arranged by size:
4090 3466 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3712 5761 1353
Size: 2359 2049 3423
Fragments arranged by size:
3423 2359 2049
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3982 4223 6418 6792 6938 387
Size: 1064 138 2393 241 2195 374 146 1280
Fragments arranged by size:
2393 2195 1280 1064 374 241 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2154 2703 2735 2949 3576 3639 4058
Size: 393 522 549 32 214 627 63 419
Cuts at: 4058 4154 5894 6491 7278 7546 1239
Size: 96 1740 597 787 268 1524
Fragments arranged by size:
1740 1524 787 627 597 549 522 419
393 268 214 96 63 32
EcoNI CCTnn'n_nnAGG
Cuts at: 2084 4768 2084
Size: 2684 5147
EcoO109I rG'GnC_Cy
Cuts at: 2862 2862
Size: 7831
EcoRI G'AATT_C
Cuts at: 3449 5140 5518 3449
Size: 1691 378 5762
Fragments arranged by size:
5762 1691 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2517 2517
Size: 7831
FspI TGC'GCA
Cuts at: 6337 6337
Size: 7831
HaeII r_GCGC'y
Cuts at: 1678 4539 4703 6978 1678
Size: 2861 164 2275 2531
Fragments arranged by size:
2861 2531 2275 164
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3464 3496 3932 3964 4205
Size: 32 135 32 1892 32 436 32 241
Cuts at: 4205 4237 6472 6504 6546 6578 1373
Size: 32 2235 32 42 32 2626
Fragments arranged by size:
2626 2235 1892 436 241 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2059 2499 2530 2605 2689 5175
Size: 988 281 121 440 31 75 84 2486
Cuts at: 5175 5421 669
Size: 246 3079
Fragments arranged by size:
3079 2486 988 440 281 246 121 84
75 31
HindIII A'AGCT_T
Cuts at: 748 2096 3954 748
Size: 1348 1858 4625
Fragments arranged by size:
4625 1858 1348
HpaI GTT'AAC
Cuts at: 2499 2689 5421 2499
Size: 190 2732 4909
Fragments arranged by size:
4909 2732 190
KpnI G_GTAC'C
Cuts at: 5156 5156
Size: 7831
MfeI C'AATT_G
Cuts at: 2310 5430 2310
Size: 3120 4711
MluI A'CGCG_T
Cuts at: 7707 7707
Size: 7831
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2142 3295 3571 4326 6945 7129 1305
Size: 837 1153 276 755 2619 184 2007
Fragments arranged by size:
2619 2007 1153 837 755 276 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3695 3902 10
Size: 54 1425 343 1863 207 3939
Fragments arranged by size:
3939 1863 1425 343 207 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2841 3195 3222 3679 3896 4157 4175
Size: 648 1675 354 27 457 217 261 18
Cuts at: 4175 4229 5789 6148 6307 518
Size: 54 1560 359 159 2042
Fragments arranged by size:
2042 1675 1560 648 457 359 354 261
217 159 54 27 18
MspA1I CmG'CkG
Cuts at: 1606 2419 3436 4023 4375 4491 4609 4885 5247
Size: 813 1017 587 352 116 118 276 362
Cuts at: 5247 5924 7072 7317 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 1017 813 677 587 362 352
276 245 118 116
NaeI GCC'GGC
Cuts at: 3845 3845
Size: 7831
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 6340
Fragments arranged by size:
6340 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7831
NgoMIV G'CCGG_C
Cuts at: 3843 3843
Size: 7831
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7831
NotI GC'GGCC_GC
Cuts at: 5278 5278
Size: 7831
NspI r_CATG'y
Cuts at: 1132 1229 2214 2626 3799 5034 5189 6734 1132
Size: 97 985 412 1173 1235 155 1545 2229
Fragments arranged by size:
2229 1545 1235 1173 985 412 155 97
PasI CC'CwG_GG
Cuts at: 1348 4782 1348
Size: 3434 4397
PciI A'CATG_T
Cuts at: 6730 6730
Size: 7831
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7831
PfoI T'CCnGG_A
Cuts at: 2709 2864 2709
Size: 155 7676
PmeI GTTT'AAAC
Cuts at: 5135 5135
Size: 7831
PmlI CAC'GTG
Cuts at: 3470 3470
Size: 7831
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5900 5932 7413 7445 5900
Size: 32 1481 32 6286
Fragments arranged by size:
6286 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2862 2862
Size: 7831
PshAI GACnn'nnGTC
Cuts at: 1613 4584 1613
Size: 2971 4860
PsiI TTA'TAA
Cuts at: 5401 5401
Size: 7831
PspXI vC'TCGA_Gb
Cuts at: 4032 4032
Size: 7831
PstI C_TGCA'G
Cuts at: 830 2944 3098 5183 830
Size: 2114 154 2085 3478
Fragments arranged by size:
3478 2114 2085 154
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7831
PvuII CAG'CTG
Cuts at: 3436 4375 4609 3436
Size: 939 234 6658
Fragments arranged by size:
6658 939 234
SalI G'TCGA_C
Cuts at: 1936 5173 1936
Size: 3237 4594
SbfI CC_TGCA'GG
Cuts at: 5183 5183
Size: 7831
ScaI AGT'ACT
Cuts at: 3598 6079 7791 3598
Size: 2481 1712 3638
Fragments arranged by size:
3638 2481 1712
SexAI A'CCwGG_T
Cuts at: 4981 4981
Size: 7831
SfcI C'TryA_G
Cuts at: 826 1045 2913 2940 3094 3324 4622 4719 5092
Size: 219 1868 27 154 230 1298 97 373
Cuts at: 5092 5179 6314 6995 7186 826
Size: 87 1135 681 191 1471
Fragments arranged by size:
1868 1471 1298 1135 681 373 230 219
191 154 97 87 27
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7831
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7831
SmaI CCC'GGG
Cuts at: 1872 5158 1872
Size: 3286 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2099 2360 3734 4032 4952 5935
Size: 197 926 156 261 1374 298 920 983
Cuts at: 5935 6836 7098 820
Size: 901 262 1553
Fragments arranged by size:
1553 1374 983 926 920 901 298 262
261 197 156
SnaBI TAC'GTA
Cuts at: 493 5622 493
Size: 5129 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7831
SphI G_CATG'C
Cuts at: 1132 3799 5034 5189 1132
Size: 2667 1235 155 3774
Fragments arranged by size:
3774 2667 1235 155
SspI AAT'ATT
Cuts at: 5 52 2830 5755 5
Size: 47 2778 2925 2081
Fragments arranged by size:
2925 2778 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7831
StyI C'CwwG_G
Cuts at: 513 1065 2004 3028 4342 5270 513
Size: 552 939 1024 1314 928 3074
Fragments arranged by size:
3074 1314 1024 939 928 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2306 3593 4538 5858 5875 6028 6213
Size: 1255 726 1287 945 1320 17 153 185
Cuts at: 6213 6658 325
Size: 445 1498
Fragments arranged by size:
1498 1320 1287 1255 945 726 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3035 3497 3596
Size: 274 80 33 51 1443 1058 462 99
Cuts at: 3596 6077 7789 96
Size: 2481 1712 138
Fragments arranged by size:
2481 1712 1443 1058 462 274 138 99
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2860 2892 4818 4850 4854 4886 7791 7823 2860
Size: 32 1926 32 4 32 2905 32 2868
Fragments arranged by size:
2905 2868 1926 32 32 32 32 4
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7831
XbaI T'CTAG_A
Cuts at: 3457 5167 3457
Size: 1710 6121
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2311 3587 2311
Size: 1276 6555
XhoI C'TCGA_G
Cuts at: 1943 4032 1943
Size: 2089 5742
XmaI C'CCGG_G
Cuts at: 1870 5156 1870
Size: 3286 4545
XmnI GAAnn'nnTTC
Cuts at: 4071 4277 5960 4071
Size: 206 1683 5942
Fragments arranged by size:
5942 1683 206
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3948 5639 276
Size: 53 83 186 936 2414 1691 2468
Fragments arranged by size:
2468 2414 1691 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI
PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI
KasI NarI NruI NsiI PacI PspOMI PsrI RsrII
SacI SacII SanDI SapI SfiI SfoI SrfI SwaI