Restriction map for
pFN21AB3244
(Circular) (Six-base) MAPSORT of: pFN21AB3244.seq Check: 9350 from: 1 to: 11191
pFN21AB3244 11191 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 5279 6243 7047 7487 5279
Size: 964 804 440 8983
Fragments arranged by size:
8983 964 804 440
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 7875 9001 278
Size: 53 83 186 936 6339 1126 2468
Fragments arranged by size:
6339 2468 1126 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2069 2361 3774 4641 8534 1937
Size: 132 292 1413 867 3893 4594
Fragments arranged by size:
4594 3893 1413 867 292 132
Acc65I G'GTAC_C
Cuts at: 2479 2611 3651 5061 7216 8512 2479
Size: 132 1040 1410 2155 1296 5158
Fragments arranged by size:
5158 2155 1410 1296 1040 132
AclI AA'CG_TT
Cuts at: 1563 9318 9691 1563
Size: 7755 373 3063
Fragments arranged by size:
7755 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2980 3018 3293 3806 4860 4953 6258 6500
Size: 1348 38 275 513 1054 93 1305 242
Cuts at: 6500 7715 8078 9254 10638 10906 1632
Size: 1215 363 1176 1384 268 1917
Fragments arranged by size:
1917 1384 1348 1305 1215 1176 1054 513
363 275 268 242 93 38
AfeI AGC'GCT
Cuts at: 5141 6638 5141
Size: 1497 9694
AflII C'TTAA_G
Cuts at: 820 1017 2659 820
Size: 197 1642 9352
Fragments arranged by size:
9352 1642 197
AflIII A'CryG_T
Cuts at: 1203 4767 5467 10090 11067 1203
Size: 3564 700 4623 977 1327
Fragments arranged by size:
4623 3564 1327 977 700
AgeI A'CCGG_T
Cuts at: 10022 10022
Size: 11191
AhdI GACnn_n'nnGTC
Cuts at: 5029 9920 5029
Size: 4891 6300
AleI CACnn'nnGTG
Cuts at: 1166 2298 3518 6311 7061 8045 8234 1166
Size: 1132 1220 2793 750 984 189 4123
Fragments arranged by size:
4123 2793 1220 1132 984 750 189
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3974 4076 5442 1184
Size: 2790 102 1366 6933
Fragments arranged by size:
6933 2790 1366 102
AlwNI CAG_nnn'CTG
Cuts at: 2295 3340 3638 4495 6287 6379 6389 7213 7289
Size: 1045 298 857 1792 92 10 824 76
Cuts at: 7289 7525 7984 8934 10506 2295
Size: 236 459 950 1572 2980
Fragments arranged by size:
2980 1792 1572 1045 950 857 824 459
298 236 92 76 10
ApaLI G'TGCA_C
Cuts at: 2975 6109 6230 6644 9248 10404 2975
Size: 3134 121 414 2604 1156 3762
Fragments arranged by size:
3762 3134 2604 1156 414 121
ApoI r'AATT_y
Cuts at: 1493 3236 3610 4664 8198 8500 8726 8878 10987
Size: 1743 374 1054 3534 302 226 152 2109
Cuts at: 10987 1493
Size: 1697
Fragments arranged by size:
3534 2109 1743 1697 1054 374 302 226
152
AseI AT'TA_AT
Cuts at: 160 1032 9745 160
Size: 872 8713 1606
Fragments arranged by size:
8713 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 11191
AvaI C'yCGr_G
Cuts at: 1870 1943 2114 2239 2367 3247 3595 4829 5027
Size: 73 171 125 128 880 348 1234 198
Cuts at: 5027 7222 8334 8516 1870
Size: 2195 1112 182 4545
Fragments arranged by size:
4545 2195 1234 1112 880 348 198 182
171 128 125 73
AvrII C'CTAG_G
Cuts at: 6133 6133
Size: 11191
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2595 2628 5053 5086 2595
Size: 33 2425 33 8700
Fragments arranged by size:
8700 2425 33 33
BamHI G'GATC_C
Cuts at: 2535 8407 8521 8888 2535
Size: 5872 114 367 4838
Fragments arranged by size:
5872 4838 367 114
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2479 2525 2611 3651 4583
Size: 325 217 627 692 46 86 1040 932
Cuts at: 4583 4922 5061 5602 6392 6452 7157 7216 7475
Size: 339 139 541 790 60 705 59 259
Cuts at: 7475 7887 8128 8183 8512 9967 618
Size: 412 241 55 329 1455 1842
Fragments arranged by size:
1842 1455 1040 932 790 705 692 627
541 412 339 329 325 259 241 217
139 86 60 59 55 46
BanII G_rGCy'C
Cuts at: 1391 2858 2932 3206 3254 3698 3726 3969 4302
Size: 1467 74 274 48 444 28 243 333
Cuts at: 4302 4830 5019 8510 1391
Size: 528 189 3491 4072
Fragments arranged by size:
4072 3491 1467 528 444 333 274 243
189 74 48 28
BbeI G_GCGC'C
Cuts at: 6396 6396
Size: 11191
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 10220
BbvCI CC'TCA_GC
Cuts at: 2598 7269 7282 7410 2598
Size: 4671 13 128 6379
Fragments arranged by size:
6379 4671 128 13
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2565 2599 6845 6879 9382 9416 1903
Size: 34 628 34 4246 34 2503 34 3678
Fragments arranged by size:
4246 3678 2503 628 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2713 9083 10293 2713
Size: 6370 1210 3611
Fragments arranged by size:
6370 3611 1210
BclI T'GATC_A
Cuts at: 1548 3300 4186 7941 1548
Size: 1752 886 3755 4798
Fragments arranged by size:
4798 3755 1752 886
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2792 3569
Size: 107 122 71 631 605 337 783 777
Cuts at: 3569 3902 8171 9802 136
Size: 333 4269 1631 1525
Fragments arranged by size:
4269 1631 1525 783 777 631 605 337
333 122 107 71
BglII A'GATC_T
Cuts at: 2148 4794 11186 2148
Size: 2646 6392 2153
Fragments arranged by size:
6392 2646 2153
BlpI GC'TnA_GC
Cuts at: 5093 5726 6624 6983 7790 8608 5093
Size: 633 898 359 807 818 7676
Fragments arranged by size:
7676 898 818 807 633 359
Bme1580I G_kGCm'C
Cuts at: 1790 2530 2979 3515 4586 4925 5939 6090 6113
Size: 740 449 536 1071 339 1014 151 23
Cuts at: 6113 6234 6455 6648 7160 9252 10408 1790
Size: 121 221 193 512 2092 1156 2573
Fragments arranged by size:
2573 2092 1156 1071 1014 740 536 512
449 339 221 193 151 121 23
BmgBI CAC'GTC
Cuts at: 1318 5224 5641 1318
Size: 3906 417 6868
Fragments arranged by size:
6868 3906 417
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2688 2935 4505
Size: 416 53 83 399 24 1271 247 1570
Cuts at: 4505 5513 5679 8095 8922 9880 442
Size: 1008 166 2416 827 958 1753
Fragments arranged by size:
2416 1753 1570 1271 1008 958 827 416
399 247 166 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 3944 5969 1056
Size: 2888 2025 6278
Fragments arranged by size:
6278 2888 2025
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2926 3742 6520 6826 7447 2926
Size: 816 2778 306 621 6670
Fragments arranged by size:
6670 2778 816 621 306
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2317 3252 5156 5372 5604 5840 5978 6785
Size: 1078 935 1904 216 232 236 138 807
Cuts at: 6785 7097 7920 9851 1239
Size: 312 823 1931 2579
Fragments arranged by size:
2579 1931 1904 1078 935 823 807 312
236 232 216 138
Bpu10I CC'TnA_GC
Cuts at: 2598 2782 3205 3963 3968 4049 5147 5211 6749
Size: 184 423 758 5 81 1098 64 1538
Cuts at: 6749 6801 7269 7282 7410 2598
Size: 52 468 13 128 6379
Fragments arranged by size:
6379 1538 1098 758 468 423 184 128
81 64 52 13 5
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2543 5699 9316 10181 10479 2543
Size: 3156 3617 865 298 3255
Fragments arranged by size:
3617 3255 3156 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2222 2406 2494 2657 3518 5124 6491
Size: 617 723 184 88 163 861 1606 1367
Cuts at: 6491 6932 9854 882
Size: 441 2922 2219
Fragments arranged by size:
2922 2219 1606 1367 861 723 617 441
184 163 88
BsaAI yAC'GTr
Cuts at: 493 1204 2251 3398 3846 5468 6658 8125 8982
Size: 711 1047 1147 448 1622 1190 1467 857
Cuts at: 8982 493
Size: 2702
Fragments arranged by size:
2702 1622 1467 1190 1147 1047 857 711
448
BsaBI GATnn'nnATC
Cuts at: 4817 11185 4817
Size: 6368 4823
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3825 4638 5883 6025
Size: 53 83 186 936 2292 813 1245 142
Cuts at: 6025 6351 6393 7872 8998 9380 10015 275
Size: 326 42 1479 1126 382 635 1451
Fragments arranged by size:
2292 1479 1451 1245 1126 936 813 635
382 326 186 142 83 53 42
BsaWI w'CCGG_w
Cuts at: 9624 10022 10296 10443 10854 9624
Size: 398 274 147 411 9961
Fragments arranged by size:
9961 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2015 2045 2669 2699 3573
Size: 30 165 30 612 30 624 30 874
Cuts at: 3573 3603 3791 3821 5586 5616 6884 6914 7070
Size: 30 188 30 1765 30 1268 30 156
Cuts at: 7070 7100 7770 7800 1178
Size: 30 670 30 4569
Fragments arranged by size:
4569 1765 1268 874 670 624 612 188
165 156 30 30 30 30 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2922 3734 4148 5930 6686 6908 7427 8024
Size: 1736 812 414 1782 756 222 519 597
Cuts at: 8024 8228 1186
Size: 204 4149
Fragments arranged by size:
4149 1782 1736 812 756 597 519 414
222 204
BseYI C'CCAG_C
Cuts at: 2288 2407 3089 3646 3742 4007 4356 4918 6014
Size: 119 682 557 96 265 349 562 1096
Cuts at: 6014 6296 7097 7774 7893 7949 8014 8135 8479
Size: 282 801 677 119 56 65 121 344
Cuts at: 8479 10394 10890 2288
Size: 1915 496 2589
Fragments arranged by size:
2589 1915 1096 801 682 677 562 557
496 349 344 282 265 121 119 119
96 65 56
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3344 3690 4310 7529 7553 7688 7868
Size: 573 1608 346 620 3219 24 135 180
Cuts at: 7868 1163
Size: 4486
Fragments arranged by size:
4486 3219 1608 620 573 346 180 135
24
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4315 8641 9402 10430 1540
Size: 275 186 2314 4326 761 1028 2301
Fragments arranged by size:
4326 2314 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2160 2979 3021 3726 3969 4302 4463 4830 5019
Size: 819 42 705 243 333 161 367 189
Cuts at: 5019 6113 6234 6648 7736 7791 7884 7971 8241
Size: 1094 121 414 1088 55 93 87 270
Cuts at: 8241 9252 9337 10408 2160
Size: 1011 85 1071 2943
Fragments arranged by size:
2943 1094 1088 1071 1011 819 705 414
367 333 270 243 189 161 121 93
87 85 55 42
BsiWI C'GTAC_G
Cuts at: 5464 5464
Size: 11191
BsmI GAATG_Cn'
Cuts at: 3871 5290 5296 5462 8702 8795 3871
Size: 1419 6 166 3240 93 6267
Fragments arranged by size:
6267 3240 1419 166 93 6
BsmBI CGTCTCn'nnnn_
Cuts at: 3816 5170 6032 3816
Size: 1354 862 8975
Fragments arranged by size:
8975 1354 862
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2160 2530 2858 2932 2979 3021 3206
Size: 399 370 370 328 74 47 42 185
Cuts at: 3206 3254 3515 3698 3726 3969 4302 4463 4586
Size: 48 261 183 28 243 333 161 123
Cuts at: 4586 4830 4925 5019 5939 6090 6113 6234 6455
Size: 244 95 94 920 151 23 121 221
Cuts at: 6455 6648 7160 7736 7791 7884 7971 8241 8510
Size: 193 512 576 55 93 87 270 269
Cuts at: 8510 9252 9337 10408 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 920 742 576 512 399 370
370 333 328 270 269 261 244 243
221 193 185 183 161 151 123 121
95 94 93 87 85 74 55 48
47 42 28 23
BspEI T'CCGG_A
Cuts at: 10854 10854
Size: 11191
BspHI T'CATG_A
Cuts at: 8328 9080 10810 8328
Size: 752 1730 8709
Fragments arranged by size:
8709 1730 752
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4407 5279 6243 6728 7005 7047 7198 7487
Size: 3563 872 964 485 277 42 151 289
Cuts at: 7487 8546 844
Size: 1059 3489
Fragments arranged by size:
3563 3489 1059 964 872 485 289 277
151 42
BsrBI CCG'CTC
Cuts at: 1394 1823 3121 5097 5356 7678 9078 1394
Size: 429 1298 1976 259 2322 1400 3507
Fragments arranged by size:
3507 2322 1976 1400 1298 429 259
BsrDI GCAATG_nn'
Cuts at: 65 1248 5424 9686 9860 65
Size: 1183 4176 4262 174 1396
Fragments arranged by size:
4262 4176 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2096 5681 6021 6978 9835 10022 1700
Size: 110 286 3585 340 957 2857 187 2869
Fragments arranged by size:
3585 2869 2857 957 340 286 187 110
BsrGI T'GTAC_A
Cuts at: 96 8448 96
Size: 8352 2839
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 11191
BssSI C'ACGA_G
Cuts at: 3734 9251 10263 3734
Size: 5517 1012 4662
Fragments arranged by size:
5517 4662 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3677 7009 8368 794
Size: 2883 3332 1359 3617
Fragments arranged by size:
3617 3332 2883 1359
BstBI TT'CG_AA
Cuts at: 4606 9995 4606
Size: 5389 5802
BstEII G'GTnAC_C
Cuts at: 1184 2675 5317 8051 1184
Size: 1491 2642 2734 4324
Fragments arranged by size:
4324 2734 2642 1491
BstXI CCAn_nnnn'nTGG
Cuts at: 2054 2888 2925 4882 5350 5503 8479 2054
Size: 834 37 1957 468 153 2976 4766
Fragments arranged by size:
4766 2976 1957 834 468 153 37
BstYI r'GATC_y
Cuts at: 1972 2148 2535 2571 2754 4500 4794 5742 6675
Size: 176 387 36 183 1746 294 948 933
Cuts at: 6675 7072 8407 8521 8888 9273 9290 10742 10828
Size: 397 1335 114 367 385 17 1452 86
Cuts at: 10828 10840 11186 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1746 1452 1335 948 933 397 387
385 367 346 294 183 176 114 86
36 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2504 2887 4375 4857 4887 5055
Size: 552 939 500 383 1488 482 30 168
Cuts at: 5055 5181 5232 6309 6456 6600 7330 7764 8179
Size: 126 51 1077 147 144 730 434 415
Cuts at: 8179 513
Size: 3525
Fragments arranged by size:
3525 1488 1077 939 730 552 500 482
434 415 383 168 147 144 126 51
30
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4330 5245 487
Size: 683 56 3104 915 6433
Fragments arranged by size:
6433 3104 915 683 56
BtsI GCAGTG_nn'
Cuts at: 2912 4518 4913 5337 7652 7911 7924 8707 9501
Size: 1606 395 424 2315 259 13 783 794
Cuts at: 9501 9521 2912
Size: 20 4582
Fragments arranged by size:
4582 2315 1606 794 783 424 395 259
20 13
ClaI AT'CG_AT
Cuts at: 1554 6099 7200 1554
Size: 4545 1101 5545
Fragments arranged by size:
5545 4545 1101
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 11156
DraI TTT'AAA
Cuts at: 8495 8495
Size: 11191
DraIII CAC_nnn'GTG
Cuts at: 4195 5507 4195
Size: 1312 9879
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2033 6527 10198 809
Size: 507 717 4494 3671 1802
Fragments arranged by size:
4494 3671 1802 717 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3223 3342 3894
Size: 54 1425 50 275 18 1393 119 552
Cuts at: 3894 4544 4884 5526 5679 6069 6459 7250 7354
Size: 650 340 642 153 390 390 791 104
Cuts at: 7354 7914 8638 9527 11156 8
Size: 560 724 889 1629 43
Fragments arranged by size:
1629 1425 1393 889 791 724 650 642
560 552 390 390 340 275 153 119
104 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 8638 1537
Size: 275 6826 4090
Fragments arranged by size:
6826 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2660 2942 4088 6037 9121 1353
Size: 1307 282 1146 1949 3084 3423
Fragments arranged by size:
3423 3084 1949 1307 1146 282
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 7154 8417 9778 10152 10298 387
Size: 1064 138 5565 1263 1361 374 146 1280
Fragments arranged by size:
5565 1361 1280 1263 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3724 3967 4300 4828 5017 3724
Size: 243 333 528 189 9898
Fragments arranged by size:
9898 528 333 243 189
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2317 2980 3018 3252 3293 3806 4860
Size: 393 685 663 38 234 41 513 1054
Cuts at: 4860 4953 5156 5372 5604 5840 5978 6258 6500
Size: 93 203 216 232 236 138 280 242
Cuts at: 6500 6785 7097 7715 7920 8078 9254 9851 10638
Size: 285 312 618 205 158 1176 597 787
Cuts at: 10638 10906 1239
Size: 268 1524
Fragments arranged by size:
1524 1176 1054 787 685 663 618 597
513 393 312 285 280 268 242 236
234 232 216 205 203 158 138 93
41 38
EcoNI CCTnn'n_nnAGG
Cuts at: 3275 3712 7137 3275
Size: 437 3425 7329
Fragments arranged by size:
7329 3425 437
EcoO109I rG'GnC_Cy
Cuts at: 2119 3211 3268 3505 4045 4150 5405 6130 6423
Size: 1092 57 237 540 105 1255 725 293
Cuts at: 6423 6776 6912 7530 7981 2119
Size: 353 136 618 451 5329
Fragments arranged by size:
5329 1255 1092 725 618 540 451 353
293 237 136 105 57
EcoRI G'AATT_C
Cuts at: 8198 8500 8878 8198
Size: 302 378 10511
Fragments arranged by size:
10511 378 302
EcoRV GAT'ATC
Cuts at: 3058 5335 6103 3058
Size: 2277 768 8146
Fragments arranged by size:
8146 2277 768
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 6283 6283
Size: 11191
FspI TGC'GCA
Cuts at: 4157 8144 9697 4157
Size: 3987 1553 5651
Fragments arranged by size:
5651 3987 1553
HaeII r_GCGC'y
Cuts at: 1678 2866 3136 5143 6396 6640 6772 8222 10338
Size: 1188 270 2007 1253 244 132 1450 2116
Cuts at: 10338 1678
Size: 2531
Fragments arranged by size:
2531 2116 2007 1450 1253 1188 270 244
132
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2015 2047 2669 2701 3142
Size: 32 135 32 443 32 622 32 441
Cuts at: 3142 3174 3529 3561 4781 4813 5015 5047 7610
Size: 32 355 32 1220 32 202 32 2563
Cuts at: 7610 7642 9832 9864 9906 9938 1373
Size: 32 2190 32 42 32 2626
Fragments arranged by size:
2626 2563 2190 1220 622 443 441 355
202 135 42 32 32 32 32 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2070 6175 8380 8535 8781 669
Size: 988 281 132 4105 2205 155 246 3079
Fragments arranged by size:
4105 3079 2205 988 281 246 155 132
HindIII A'AGCT_T
Cuts at: 748 6194 6482 8292 748
Size: 5446 288 1810 3647
Fragments arranged by size:
5446 3647 1810 288
HpaI GTT'AAC
Cuts at: 8781 8781
Size: 11191
KasI G'GCGC_C
Cuts at: 6392 6392
Size: 11191
KpnI G_GTAC'C
Cuts at: 2483 2615 3655 5065 7220 8516 2483
Size: 132 1040 1410 2155 1296 5158
Fragments arranged by size:
5158 2155 1410 1296 1040 132
MfeI C'AATT_G
Cuts at: 8790 8790
Size: 11191
MluI A'CGCG_T
Cuts at: 11067 11067
Size: 11191
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2001 4200 4253 7416 7942 10305 10489 1305
Size: 696 2199 53 3163 526 2363 184 2007
Fragments arranged by size:
3163 2363 2199 2007 696 526 184 53
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3225 3896 4546 4886 5528
Size: 54 1425 343 1393 671 650 340 642
Cuts at: 5528 7252 10
Size: 1724 3949
Fragments arranged by size:
3949 1724 1425 1393 671 650 642 343
340 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2298 3518 3860 4727 4886 5301 6311
Size: 648 1132 1220 342 867 159 415 1010
Cuts at: 6311 7061 8045 8234 8456 9149 9508 9667 518
Size: 750 984 189 222 693 359 159 2042
Fragments arranged by size:
2042 1220 1132 1010 984 867 750 693
648 415 359 342 222 189 159 159
MspA1I CmG'CkG
Cuts at: 1606 3089 3638 4259 4317 4360 4538 4870 5443
Size: 1483 549 621 58 43 178 332 573
Cuts at: 5443 5700 5861 5897 5947 6074 6287 7409 8181
Size: 257 161 36 50 127 213 1122 772
Cuts at: 8181 8607 9284 10432 10677 1606
Size: 426 677 1148 245 2120
Fragments arranged by size:
2120 1483 1148 1122 772 677 621 573
549 426 332 257 245 213 178 161
127 58 50 43 36
NaeI GCC'GGC
Cuts at: 5683 6023 6980 5683
Size: 340 957 9894
Fragments arranged by size:
9894 957 340
NarI GG'CG_CC
Cuts at: 6393 6393
Size: 11191
NcoI C'CATG_G
Cuts at: 513 1065 2004 2887 4857 4887 6309 7764 513
Size: 552 939 883 1970 30 1422 1455 3940
Fragments arranged by size:
3940 1970 1455 1422 939 883 552 30
NdeI CA'TA_TG
Cuts at: 387 6650 387
Size: 6263 4928
NgoMIV G'CCGG_C
Cuts at: 5681 6021 6978 5681
Size: 340 957 9894
Fragments arranged by size:
9894 957 340
NheI G'CTAG_C
Cuts at: 1052 3940 5965 1052
Size: 2888 2025 6278
Fragments arranged by size:
6278 2888 2025
NotI GC'GGCC_GC
Cuts at: 8638 8638
Size: 11191
NspI r_CATG'y
Cuts at: 1132 1229 3590 4355 4771 5279 5378 6173 7108
Size: 97 2361 765 416 508 99 795 935
Cuts at: 7108 8149 8449 8549 10094 1132
Size: 1041 300 100 1545 2229
Fragments arranged by size:
2361 2229 1545 1041 935 795 765 508
416 300 100 99 97
PasI CC'CwG_GG
Cuts at: 1348 2616 3098 3146 3334 3682 6929 7340 1348
Size: 1268 482 48 188 348 3247 411 5199
Fragments arranged by size:
5199 3247 1268 482 411 348 188 48
PciI A'CATG_T
Cuts at: 4767 10090 4767
Size: 5323 5868
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2295 2776 3340 5056 6379 6538 6823 6889
Size: 1187 481 564 1716 1323 159 285 66
Cuts at: 6889 7525 1108
Size: 636 4774
Fragments arranged by size:
4774 1716 1323 1187 636 564 481 285
159 66
PfoI T'CCnGG_A
Cuts at: 5202 5955 5202
Size: 753 10438
PmeI GTTT'AAAC
Cuts at: 8495 8495
Size: 11191
PmlI CAC'GTG
Cuts at: 6658 6658
Size: 11191
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 9260 9292 10773 10805 9260
Size: 32 1481 32 9646
Fragments arranged by size:
9646 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3505 4045 5405 7530 3505
Size: 540 1360 2125 7166
Fragments arranged by size:
7166 2125 1360 540
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 11191
PsiI TTA'TAA
Cuts at: 8761 8761
Size: 11191
PspXI vC'TCGA_Gb
Cuts at: 5027 5027
Size: 11191
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 8432 8464 8432
Size: 32 11159
PstI C_TGCA'G
Cuts at: 830 2331 5421 6472 7005 8543 830
Size: 1501 3090 1051 533 1538 3478
Fragments arranged by size:
3478 3090 1538 1501 1051 533
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 11191
PvuII CAG'CTG
Cuts at: 3089 4259 4360 4538 4870 5443 5861 5897 7409
Size: 1170 101 178 332 573 418 36 1512
Cuts at: 7409 3089
Size: 6871
Fragments arranged by size:
6871 1512 1170 573 418 332 178 101
36
SacI G_AGCT'C
Cuts at: 3726 3969 4302 4830 5019 3726
Size: 243 333 528 189 9898
Fragments arranged by size:
9898 528 333 243 189
SacII CC_GC'GG
Cuts at: 8182 8182
Size: 11191
SalI G'TCGA_C
Cuts at: 1936 2068 8533 1936
Size: 132 6465 4594
Fragments arranged by size:
6465 4594 132
SanDI GG'GwC_CC
Cuts at: 7530 7530
Size: 11191
SapI GCTCTTCn'nnn_
Cuts at: 4088 4088
Size: 11191
SbfI CC_TGCA'GG
Cuts at: 8543 8543
Size: 11191
ScaI AGT'ACT
Cuts at: 5668 9439 11151 5668
Size: 3771 1712 5708
Fragments arranged by size:
5708 3771 1712
SexAI A'CCwGG_T
Cuts at: 6244 6244
Size: 11191
SfcI C'TryA_G
Cuts at: 826 1045 2327 4680 5393 5417 5833 6468 7001
Size: 219 1282 2353 713 24 416 635 533
Cuts at: 7001 8539 9674 10355 10546 826
Size: 1538 1135 681 191 1471
Fragments arranged by size:
2353 1538 1471 1282 1135 713 681 635
533 416 219 191 24
SfiI GGCCn_nnn'nGGCC
Cuts at: 3902 8171 3902
Size: 4269 6922
SfoI GGC'GCC
Cuts at: 6394 6394
Size: 11191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 11191
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 11191
SmaI CCC'GGG
Cuts at: 1872 7224 8336 8518 1872
Size: 5352 1112 182 4545
Fragments arranged by size:
5352 4545 1112 182
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2558 2659 4829 5027 5714 9295
Size: 197 926 615 101 2170 198 687 3581
Cuts at: 9295 10196 10458 820
Size: 901 262 1553
Fragments arranged by size:
3581 2170 1553 926 901 687 615 262
198 197 101
SnaBI TAC'GTA
Cuts at: 493 3398 8982 493
Size: 2905 5584 2702
Fragments arranged by size:
5584 2905 2702
SpeI A'CTAG_T
Cuts at: 152 7295 152
Size: 7143 4048
SphI G_CATG'C
Cuts at: 1132 5378 8149 8549 1132
Size: 4246 2771 400 3774
Fragments arranged by size:
4246 3774 2771 400
SspI AAT'ATT
Cuts at: 5 52 3313 9115 5
Size: 47 3261 5802 2081
Fragments arranged by size:
5802 3261 2081 47
StuI AGG'CCT
Cuts at: 1517 4139 1517
Size: 2622 8569
StyI C'CwwG_G
Cuts at: 513 1065 2004 2887 3471 4627 4857 4887 5430
Size: 552 939 883 584 1156 230 30 543
Cuts at: 5430 5523 6045 6133 6309 6418 6447 6888 7116
Size: 93 522 88 176 109 29 441 228
Cuts at: 7116 7614 7764 8630 513
Size: 498 150 866 3074
Fragments arranged by size:
3074 1156 939 883 866 584 552 543
522 498 441 230 228 176 150 109
93 88 30 29
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2023 2204 3027 3554 3674 4604 4850
Size: 1255 443 181 823 527 120 930 246
Cuts at: 4850 5495 5639 7991 8039 8114 8167 9218 9235
Size: 645 144 2352 48 75 53 1051 17
Cuts at: 9235 9388 9573 10018 325
Size: 153 185 445 1498
Fragments arranged by size:
2352 1498 1255 1051 930 823 645 527
445 443 246 185 181 153 144 120
75 53 48 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5666 8074 8448
Size: 274 80 33 51 1443 3689 2408 374
Cuts at: 8448 9437 11149 96
Size: 989 1712 138
Fragments arranged by size:
3689 2408 1712 1443 989 374 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3573 3605 3898 3930 4602 4634 5586 5618 5918
Size: 32 293 32 672 32 952 32 300
Cuts at: 5918 5950 6818 6850 6884 6909 6916 6941 7070
Size: 32 868 32 34 25 7 25 129
Cuts at: 7070 7102 7770 7802 11151 11183 3573
Size: 32 668 32 3349 32 3581
Fragments arranged by size:
3581 3349 952 868 672 668 300 293
129 34 32 32 32 32 32 32
32 32 32 25 25 7
Tth111I GACn'n_nGTC
Cuts at: 1316 3350 7874 1316
Size: 2034 4524 4633
Fragments arranged by size:
4633 4524 2034
XbaI T'CTAG_A
Cuts at: 8527 8527
Size: 11191
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3943 6121 7771 8347 3943
Size: 2178 1650 576 6787
Fragments arranged by size:
6787 2178 1650 576
XhoI C'TCGA_G
Cuts at: 1943 4829 5027 1943
Size: 2886 198 8107
Fragments arranged by size:
8107 2886 198
XmaI C'CCGG_G
Cuts at: 1870 7222 8334 8516 1870
Size: 5352 1112 182 4545
Fragments arranged by size:
5352 4545 1112 182
XmnI GAAnn'nnTTC
Cuts at: 4958 9320 4958
Size: 4362 6829
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 7873 8999 276
Size: 53 83 186 936 6339 1126 2468
Fragments arranged by size:
6339 2468 1126 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspXI PsrI PstI PvuI PvuII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI BfrBI BstZ17I Bsu36I FseI FspAI
NruI NsiI PacI PspOMI RsrII SrfI SwaI