Restriction map for
pFN21AB3241
(Circular) (Six-base) MAPSORT of: pFN21AB3241.seq Check: 2764 from: 1 to: 7582
pFN21AB3241 7582 bp
With 182 enzymes: SgfI *
October 20, 2009 10:33 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5392 278
Size: 53 83 186 936 3856 2468
Fragments arranged by size:
3856 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4863 4925 1937
Size: 2926 62 4594
Fragments arranged by size:
4594 2926 62
Acc65I G'GTAC_C
Cuts at: 4903 4903
Size: 7582
AclI AA'CG_TT
Cuts at: 1563 5709 6082 1563
Size: 4146 373 3063
Fragments arranged by size:
4146 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2306 2843 3308 3600 3681 5645 7029 7297
Size: 674 537 465 292 81 1964 1384 268
Cuts at: 7297 1632
Size: 1917
Fragments arranged by size:
1964 1917 1384 674 537 465 292 268
81
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7385
AflIII A'CryG_T
Cuts at: 1203 6481 7458 1203
Size: 5278 977 1327
Fragments arranged by size:
5278 1327 977
AgeI A'CCGG_T
Cuts at: 6413 6413
Size: 7582
AhdI GACnn_n'nnGTC
Cuts at: 2420 6311 2420
Size: 3891 3691
AleI CACnn'nnGTG
Cuts at: 1166 3885 1166
Size: 2719 4863
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2855 1184
Size: 1671 5911
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4510 4542 4510
Size: 32 7550
AlwNI CAG_nnn'CTG
Cuts at: 3358 3551 5325 6897 3358
Size: 193 1774 1572 4043
Fragments arranged by size:
4043 1774 1572 193
ApaLI G'TGCA_C
Cuts at: 2725 5639 6795 2725
Size: 2914 1156 3512
Fragments arranged by size:
3512 2914 1156
ApoI r'AATT_y
Cuts at: 1493 2845 3137 3209 4138 4393 4669 4766 4891
Size: 1352 292 72 929 255 276 97 125
Cuts at: 4891 5117 5269 7378 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 1352 929 292 276 255 226
152 125 97 72
AseI AT'TA_AT
Cuts at: 160 1032 6136 160
Size: 872 5104 1606
Fragments arranged by size:
5104 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7582
AvaI C'yCGr_G
Cuts at: 1870 1943 4907 1870
Size: 73 2964 4545
Fragments arranged by size:
4545 2964 73
BamHI G'GATC_C
Cuts at: 4912 5279 4912
Size: 367 7215
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2365 3030 4903 6358 618
Size: 325 217 627 578 665 1873 1455 1842
Fragments arranged by size:
1873 1842 1455 665 627 578 325 217
BanII G_rGCy'C
Cuts at: 1391 2151 3912 4584 4901 1391
Size: 760 1761 672 317 4072
Fragments arranged by size:
4072 1761 760 672 317
BbeI G_GCGC'C
Cuts at: 3034 3034
Size: 7582
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3669 4068 928
Size: 971 1770 399 4442
Fragments arranged by size:
4442 1770 971 399
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3940 3974 5773 5807 1903
Size: 34 2003 34 1799 34 3678
Fragments arranged by size:
3678 2003 1799 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3977 5474 6684 3977
Size: 1497 1210 4875
Fragments arranged by size:
4875 1497 1210
BclI T'GATC_A
Cuts at: 1548 3433 1548
Size: 1885 5697
BfrBI ATG'CAT
Cuts at: 2516 4053 2516
Size: 1537 6045
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6193 136
Size: 107 122 71 631 605 4521 1525
Fragments arranged by size:
4521 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2794 4609 4718 7577 2794
Size: 1815 109 2859 2799
Fragments arranged by size:
2859 2799 1815 109
BlpI GC'TnA_GC
Cuts at: 4999 4999
Size: 7582
Bme1580I G_kGCm'C
Cuts at: 1790 2729 5643 6799 1790
Size: 939 2914 1156 2573
Fragments arranged by size:
2914 2573 1156 939
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7582
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4022 4837 5313
Size: 416 53 83 399 24 2605 815 476
Cuts at: 5313 6271 442
Size: 958 1753
Fragments arranged by size:
2605 1753 958 815 476 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 3047 1056
Size: 1991 5591
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2141 4697 6242 1239
Size: 902 2556 1545 2579
Fragments arranged by size:
2579 2556 1545 902
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3823 5707 6572 6870 3823
Size: 1884 865 298 4535
Fragments arranged by size:
4535 1884 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2527 4513 6245 882
Size: 617 1028 1986 1732 2219
Fragments arranged by size:
2219 1986 1732 1028 617
BsaAI yAC'GTr
Cuts at: 493 1204 5373 493
Size: 711 4169 2702
Fragments arranged by size:
4169 2702 711
BsaBI GATnn'nnATC
Cuts at: 4196 4791 7576 4196
Size: 595 2785 4202
Fragments arranged by size:
4202 2785 595
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3031 3742 4629 5389
Size: 53 83 186 936 1498 711 887 760
Cuts at: 5389 5771 6406 275
Size: 382 635 1451
Fragments arranged by size:
1498 1451 936 887 760 711 635 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 2176 6015 6413 6687 6834 7245 2176
Size: 3839 398 274 147 411 2513
Fragments arranged by size:
3839 2513 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2035 2065 4510 4540 1178
Size: 30 165 30 632 30 2445 30 4220
Fragments arranged by size:
4220 2445 632 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2062 3575 1186
Size: 876 1513 5193
Fragments arranged by size:
5193 1513 876
BseYI C'CCAG_C
Cuts at: 2211 6785 7281 2211
Size: 4574 496 2512
Fragments arranged by size:
4574 2512 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2808 3147 1163
Size: 573 1072 339 5598
Fragments arranged by size:
5598 1072 573 339
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5032 5793 6821 1540
Size: 275 186 3031 761 1028 2301
Fragments arranged by size:
3031 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2475 2729 3585 3912 4584 5643 5728 6799 2475
Size: 254 856 327 672 1059 85 1071 3258
Fragments arranged by size:
3258 1071 1059 856 672 327 254 85
BsmI GAATG_Cn'
Cuts at: 3103 3235 3385 4055 5093 5186 3103
Size: 132 150 670 1038 93 5499
Fragments arranged by size:
5499 1038 670 150 132 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4720 4720
Size: 7582
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2151 2475 2729 3585 3912 4584 4901
Size: 399 361 324 254 856 327 672 317
Cuts at: 4901 5643 5728 6799 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 856 742 672 399 361 327
324 317 254 85
BspEI T'CCGG_A
Cuts at: 7245 7245
Size: 7582
BspHI T'CATG_A
Cuts at: 5471 7201 5471
Size: 1730 5852
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4937 844
Size: 4093 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3865 5469 1394
Size: 429 2042 1604 3507
Fragments arranged by size:
3507 2042 1604 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2803 6077 6251 65
Size: 1183 1555 3274 174 1396
Fragments arranged by size:
3274 1555 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3033 4452 6226 6413 1700
Size: 110 1223 1419 1774 187 2869
Fragments arranged by size:
2869 1774 1419 1223 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7582
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7582
BssSI C'ACGA_G
Cuts at: 5642 6654 5642
Size: 1012 6570
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4353 794
Size: 3559 4023
BstBI TT'CG_AA
Cuts at: 4837 6386 4837
Size: 1549 6033
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7582
BstYI r'GATC_y
Cuts at: 1972 2794 4594 4609 4718 4912 5279 5664 5681
Size: 822 1800 15 109 194 367 385 17
Cuts at: 5681 7133 7219 7231 7577 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1800 1452 822 385 367 346 194
109 86 17 15 12
BtgI C'CryG_G
Cuts at: 513 1065 2395 2681 513
Size: 552 1330 286 5414
Fragments arranged by size:
5414 1330 552 286
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6843
Fragments arranged by size:
6843 683 56
BtsI GCAGTG_nn'
Cuts at: 3060 4354 5098 5892 5912 3060
Size: 1294 744 794 20 4730
Fragments arranged by size:
4730 1294 794 744 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7582
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2802 2837 529
Size: 35 2238 35 5274
Fragments arranged by size:
5274 2238 35 35
DraI TTT'AAA
Cuts at: 2307 4774 4886 2307
Size: 2467 112 5003
Fragments arranged by size:
5003 2467 112
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2933 6589 809
Size: 507 1617 3656 1802
Fragments arranged by size:
3656 1802 1617 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2734 4640 5029
Size: 54 1425 50 275 18 904 1906 389
Cuts at: 5029 5918 7547 8
Size: 889 1629 43
Fragments arranged by size:
1906 1629 1425 904 889 389 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5029 1537
Size: 275 3217 4090
Fragments arranged by size:
4090 3217 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2080 2463 2766 5512 1353
Size: 727 383 303 2746 3423
Fragments arranged by size:
3423 2746 727 383 303
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6169 6543 6689 387
Size: 1064 138 4580 374 146 1280
Fragments arranged by size:
4580 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3910 4582 3910
Size: 672 6910
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2141 2306 2843 3308 3600 3681 4697
Size: 393 509 165 537 465 292 81 1016
Cuts at: 4697 5645 6242 7029 7297 1239
Size: 948 597 787 268 1524
Fragments arranged by size:
1524 1016 948 787 597 537 509 465
393 292 268 165 81
EcoNI CCTnn'n_nnAGG
Cuts at: 2089 2195 2307 3887 3917 2089
Size: 106 112 1580 30 5754
Fragments arranged by size:
5754 1580 112 106 30
EcoO109I rG'GnC_Cy
Cuts at: 3726 4843 3726
Size: 1117 6465
EcoRI G'AATT_C
Cuts at: 4393 4891 5269 4393
Size: 498 378 6706
Fragments arranged by size:
6706 498 378
EcoRV GAT'ATC
Cuts at: 4159 4159
Size: 7582
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4631 4631
Size: 7582
FspI TGC'GCA
Cuts at: 6088 6088
Size: 7582
HaeII r_GCGC'y
Cuts at: 1678 3034 6729 1678
Size: 1356 3695 2531
Fragments arranged by size:
3695 2531 1356
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2406 2438 3611 3641 3643
Size: 32 135 32 834 32 1173 30 2
Cuts at: 3643 3673 6223 6255 6297 6329 1373
Size: 30 2550 32 42 32 2626
Fragments arranged by size:
2626 2550 1173 834 135 42 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 669 1657 1938 4926 5172 669
Size: 988 281 2988 246 3079
Fragments arranged by size:
3079 2988 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7582
HpaI GTT'AAC
Cuts at: 5172 5172
Size: 7582
KasI G'GCGC_C
Cuts at: 3030 3030
Size: 7582
KpnI G_GTAC'C
Cuts at: 4907 4907
Size: 7582
MfeI C'AATT_G
Cuts at: 2165 5181 2165
Size: 3016 4566
MluI A'CGCG_T
Cuts at: 7458 7458
Size: 7582
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2078 3135 3373 4189 6696 6880 1305
Size: 773 1057 238 816 2507 184 2007
Fragments arranged by size:
2507 2007 1057 816 773 238 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2736 10
Size: 54 1425 343 904 4856
Fragments arranged by size:
4856 1425 904 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2064 2408 3335 3885 5540 5899 6058
Size: 648 898 344 927 550 1655 359 159
Cuts at: 6058 518
Size: 2042
Fragments arranged by size:
2042 1655 927 898 648 550 359 344
159
MspA1I CmG'CkG
Cuts at: 1606 2211 2618 2633 4998 5675 6823 7068 1606
Size: 605 407 15 2365 677 1148 245 2120
Fragments arranged by size:
2365 2120 1148 677 605 407 245 15
NaeI GCC'GGC
Cuts at: 3035 3035
Size: 7582
NarI GG'CG_CC
Cuts at: 3031 3031
Size: 7582
NcoI C'CATG_G
Cuts at: 513 1065 2395 513
Size: 552 1330 5700
Fragments arranged by size:
5700 1330 552
NdeI CA'TA_TG
Cuts at: 387 3943 387
Size: 3556 4026
NgoMIV G'CCGG_C
Cuts at: 3033 3033
Size: 7582
NheI G'CTAG_C
Cuts at: 1052 3043 1052
Size: 1991 5591
NotI GC'GGCC_GC
Cuts at: 5029 5029
Size: 7582
NsiI A_TGCA'T
Cuts at: 2518 4055 2518
Size: 1537 6045
NspI r_CATG'y
Cuts at: 1132 1229 2896 3753 4940 6485 1132
Size: 97 1667 857 1187 1545 2229
Fragments arranged by size:
2229 1667 1545 1187 857 97
PasI CC'CwG_GG
Cuts at: 1348 4847 1348
Size: 3499 4083
PciI A'CATG_T
Cuts at: 6481 6481
Size: 7582
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4034 4238 4420 1108
Size: 2926 204 182 4270
Fragments arranged by size:
4270 2926 204 182
PfoI T'CCnGG_A
Cuts at: 2334 3912 2334
Size: 1578 6004
PmeI GTTT'AAAC
Cuts at: 4886 4886
Size: 7582
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4510 4542 5651 5683 7164 7196 4510
Size: 32 1109 32 1481 32 4896
Fragments arranged by size:
4896 1481 1109 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 4843 4843
Size: 7582
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7582
PsiI TTA'TAA
Cuts at: 5152 5152
Size: 7582
PstI C_TGCA'G
Cuts at: 830 2991 3879 4684 4934 830
Size: 2161 888 805 250 3478
Fragments arranged by size:
3478 2161 888 805 250
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7582
PvuII CAG'CTG
Cuts at: 2211 2618 2633 2211
Size: 407 15 7160
Fragments arranged by size:
7160 407 15
SacI G_AGCT'C
Cuts at: 3912 4584 3912
Size: 672 6910
SalI G'TCGA_C
Cuts at: 1936 4924 1936
Size: 2988 4594
SapI GCTCTTCn'nnn_
Cuts at: 2463 2463
Size: 7582
SbfI CC_TGCA'GG
Cuts at: 4934 4934
Size: 7582
ScaI AGT'ACT
Cuts at: 4602 5830 7542 4602
Size: 1228 1712 4642
Fragments arranged by size:
4642 1712 1228
SexAI A'CCwGG_T
Cuts at: 4522 4522
Size: 7582
SfcI C'TryA_G
Cuts at: 826 1045 2987 3270 3875 4680 4930 6065 6746
Size: 219 1942 283 605 805 250 1135 681
Cuts at: 6746 6937 826
Size: 191 1471
Fragments arranged by size:
1942 1471 1135 805 681 605 283 250
219 191
SfoI GGC'GCC
Cuts at: 3032 3032
Size: 7582
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7582
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7582
SmaI CCC'GGG
Cuts at: 1872 4909 1872
Size: 3037 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3802 5686 6587 6849 820
Size: 197 926 1859 1884 901 262 1553
Fragments arranged by size:
1884 1859 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5373 493
Size: 4880 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7582
SphI G_CATG'C
Cuts at: 1132 3753 4940 1132
Size: 2621 1187 3774
Fragments arranged by size:
3774 2621 1187
SspI AAT'ATT
Cuts at: 5 52 3454 3688 5506 5
Size: 47 3402 234 1818 2081
Fragments arranged by size:
3402 2081 1818 234 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7582
StyI C'CwwG_G
Cuts at: 513 1065 2196 2395 4033 5021 513
Size: 552 1131 199 1638 988 3074
Fragments arranged by size:
3074 1638 1131 988 552 199
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4123 5609 5626 5779 5964 6409 325
Size: 1255 2543 1486 17 153 185 445 1498
Fragments arranged by size:
2543 1498 1486 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4551 4600 5828
Size: 274 80 33 51 1443 2574 49 1228
Cuts at: 5828 7540 96
Size: 1712 138
Fragments arranged by size:
2574 1712 1443 1228 274 138 80 51
49 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7542 7574 7542
Size: 32 7550
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7582
XbaI T'CTAG_A
Cuts at: 4918 4918
Size: 7582
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7582
XmaI C'CCGG_G
Cuts at: 1870 4907 1870
Size: 3037 4545
XmnI GAAnn'nnTTC
Cuts at: 2856 3412 5711 2856
Size: 556 2299 4727
Fragments arranged by size:
4727 2299 556
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5390 276
Size: 53 83 186 936 3856 2468
Fragments arranged by size:
3856 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SacI SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbvCI BplI
Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII FseI FspAI
NruI PacI PmlI PspOMI PspXI PsrI RsrII SacII
SanDI SfiI SrfI SwaI XcmI