Restriction map for
pFN21AB3240
(Circular) (Six-base) MAPSORT of: pFN21AB3240.seq Check: 442 from: 1 to: 7324
pFN21AB3240 7324 bp
With 182 enzymes: SgfI *
February 27, 2009 14:45 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5134 278
Size: 53 83 186 936 3598 2468
Fragments arranged by size:
3598 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4667 1937
Size: 2730 4594
Acc65I G'GTAC_C
Cuts at: 3295 4645 3295
Size: 1350 5974
AclI AA'CG_TT
Cuts at: 1563 3010 5451 5824 1563
Size: 1447 2441 373 3063
Fragments arranged by size:
3063 2441 1447 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2646 2670 3737 4394 5387 6771 7039 1632
Size: 1014 24 1067 657 993 1384 268 1917
Fragments arranged by size:
1917 1384 1067 1014 993 657 268 24
AflII C'TTAA_G
Cuts at: 820 1017 4028 4358 820
Size: 197 3011 330 3786
Fragments arranged by size:
3786 3011 330 197
AflIII A'CryG_T
Cuts at: 1203 4591 6223 7200 1203
Size: 3388 1632 977 1327
Fragments arranged by size:
3388 1632 1327 977
AgeI A'CCGG_T
Cuts at: 6155 6155
Size: 7324
AhdI GACnn_n'nnGTC
Cuts at: 6053 6053
Size: 7324
AleI CACnn'nnGTG
Cuts at: 1166 2753 1166
Size: 1587 5737
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7324
AlwNI CAG_nnn'CTG
Cuts at: 2693 5067 6639 2693
Size: 2374 1572 3378
Fragments arranged by size:
3378 2374 1572
ApaLI G'TGCA_C
Cuts at: 3966 5381 6537 3966
Size: 1415 1156 4753
Fragments arranged by size:
4753 1415 1156
ApoI r'AATT_y
Cuts at: 1493 2299 2392 2818 3565 4334 4633 4859 5011
Size: 806 93 426 747 769 299 226 152
Cuts at: 5011 7120 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 806 769 747 426 299 226
152 93
AseI AT'TA_AT
Cuts at: 160 1032 5878 160
Size: 872 4846 1606
Fragments arranged by size:
4846 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7324
AvaI C'yCGr_G
Cuts at: 1870 1943 3038 4649 1870
Size: 73 1095 1611 4545
Fragments arranged by size:
4545 1611 1095 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2787 2820 2787
Size: 33 7291
BamHI G'GATC_C
Cuts at: 4654 5021 4654
Size: 367 6957
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3295 4645 6100 618
Size: 325 217 627 1508 1350 1455 1842
Fragments arranged by size:
1842 1508 1455 1350 627 325 217
BanII G_rGCy'C
Cuts at: 1391 4643 1391
Size: 3252 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4209 4273 928
Size: 971 2310 64 3979
Fragments arranged by size:
3979 2310 971 64
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5515 5549 1903
Size: 34 3578 34 3678
Fragments arranged by size:
3678 3578 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5216 6426 5216
Size: 1210 6114
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7324
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5935 136
Size: 107 122 71 631 605 4263 1525
Fragments arranged by size:
4263 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2245 7319 2245
Size: 5074 2250
BlpI GC'TnA_GC
Cuts at: 2811 3306 4741 2811
Size: 495 1435 5394
Fragments arranged by size:
5394 1435 495
Bme1580I G_kGCm'C
Cuts at: 1790 3814 3970 5385 6541 1790
Size: 2024 156 1415 1156 2573
Fragments arranged by size:
2573 2024 1415 1156 156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7324
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4318 5055 6013
Size: 416 53 83 399 24 2901 737 958
Cuts at: 6013 442
Size: 1753
Fragments arranged by size:
2901 1753 958 737 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7324
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2158 2158
Size: 7324
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2226 3613 3957 5984 1239
Size: 987 1387 344 2027 2579
Fragments arranged by size:
2579 2027 1387 987 344
Bpu10I CC'TnA_GC
Cuts at: 3675 4011 3675
Size: 336 6988
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2090 2128 2946 3186 5449 6314 6612 2090
Size: 38 818 240 2263 865 298 2802
Fragments arranged by size:
2802 2263 865 818 298 240 38
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4237 5987 882
Size: 617 2738 1750 2219
Fragments arranged by size:
2738 2219 1750 617
BsaAI yAC'GTr
Cuts at: 493 1204 5115 493
Size: 711 3911 2702
Fragments arranged by size:
3911 2702 711
BsaBI GATnn'nnATC
Cuts at: 2889 7318 2889
Size: 4429 2895
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5131 5513 6148 275
Size: 53 83 186 936 3598 382 635 1451
Fragments arranged by size:
3598 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4044 5757 6155 6429 6576 6987 4044
Size: 1713 398 274 147 411 4381
Fragments arranged by size:
4381 1713 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3127 3157 1178
Size: 30 165 30 1724 30 5345
Fragments arranged by size:
5345 1724 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2498 3065 4526 1186
Size: 1312 567 1461 3984
Fragments arranged by size:
3984 1461 1312 567
BseYI C'CCAG_C
Cuts at: 6527 7023 6527
Size: 496 6828
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3651 4004 1163
Size: 573 1915 353 4483
Fragments arranged by size:
4483 1915 573 353
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4774 5535 6563 1540
Size: 275 186 2773 761 1028 2301
Fragments arranged by size:
2773 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3970 5385 5470 6541 3970
Size: 1415 85 1071 4753
Fragments arranged by size:
4753 1415 1071 85
BsmI GAATG_Cn'
Cuts at: 2327 2494 2859 3402 4835 4928 2327
Size: 167 365 543 1433 93 4723
Fragments arranged by size:
4723 1433 543 365 167 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3814 3970 4643 5385 5470 6541 1391
Size: 399 2024 156 673 742 85 1071 2174
Fragments arranged by size:
2174 2024 1071 742 673 399 156 85
BspEI T'CCGG_A
Cuts at: 4044 6987 4044
Size: 2943 4381
BspHI T'CATG_A
Cuts at: 2161 2553 5213 6943 2161
Size: 392 2660 1730 2542
Fragments arranged by size:
2660 2542 1730 392
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3307 3905 4432 4679 844
Size: 2463 598 527 247 3489
Fragments arranged by size:
3489 2463 598 527 247
BsrBI CCG'CTC
Cuts at: 1394 1823 5211 1394
Size: 429 3388 3507
Fragments arranged by size:
3507 3388 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5819 5993 65
Size: 1183 4571 174 1396
Fragments arranged by size:
4571 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5968 6155 1700
Size: 110 4158 187 2869
Fragments arranged by size:
4158 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7324
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7324
BssSI C'ACGA_G
Cuts at: 5384 6396 5384
Size: 1012 6312
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7324
BstBI TT'CG_AA
Cuts at: 6128 6128
Size: 7324
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7324
BstXI CCAn_nnnn'nTGG
Cuts at: 3176 3176
Size: 7324
BstYI r'GATC_y
Cuts at: 1972 2059 2245 4654 5021 5406 5423 6875 6961
Size: 87 186 2409 367 385 17 1452 86
Cuts at: 6961 6973 7319 1972
Size: 12 346 1977
Fragments arranged by size:
2409 1977 1452 385 367 346 186 87
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 4223 4223
Size: 7324
BtgI C'CryG_G
Cuts at: 513 1065 2004 3642 513
Size: 552 939 1638 4195
Fragments arranged by size:
4195 1638 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6585
Fragments arranged by size:
6585 683 56
BtsI GCAGTG_nn'
Cuts at: 4840 5634 5654 4840
Size: 794 20 6510
Fragments arranged by size:
6510 794 20
ClaI AT'CG_AT
Cuts at: 1554 3840 1554
Size: 2286 5038
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7289
DraI TTT'AAA
Cuts at: 3015 3721 3879 4339 4628 3015
Size: 706 158 460 289 5711
Fragments arranged by size:
5711 706 460 289 158
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6331 809
Size: 507 5015 1802
Fragments arranged by size:
5015 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4055 4083 4771
Size: 54 1425 50 275 18 2225 28 688
Cuts at: 4771 5660 7289 8
Size: 889 1629 43
Fragments arranged by size:
2225 1629 1425 889 688 275 54 50
43 28 18
EagI C'GGCC_G
Cuts at: 1537 1812 4771 1537
Size: 275 2959 4090
Fragments arranged by size:
4090 2959 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2148 2382 2622 3826 3879 4062 4255 4536
Size: 795 234 240 1204 53 183 193 281
Cuts at: 4536 5254 1353
Size: 718 3423
Fragments arranged by size:
3423 1204 795 718 281 240 234 193
183 53
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5911 6285 6431 387
Size: 1064 138 4322 374 146 1280
Fragments arranged by size:
4322 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2226 2646 2670 3613 3737 3957 4394
Size: 393 594 420 24 943 124 220 437
Cuts at: 4394 5387 5984 6771 7039 1239
Size: 993 597 787 268 1524
Fragments arranged by size:
1524 993 943 787 597 594 437 420
393 268 220 124 24
EcoNI CCTnn'n_nnAGG
Cuts at: 3975 3975
Size: 7324
EcoO109I rG'GnC_Cy
Cuts at: 2096 2096
Size: 7324
EcoRI G'AATT_C
Cuts at: 2818 4633 5011 2818
Size: 1815 378 5131
Fragments arranged by size:
5131 1815 378
EcoRV GAT'ATC
Cuts at: 2079 2079
Size: 7324
FspI TGC'GCA
Cuts at: 5830 5830
Size: 7324
HaeII r_GCGC'y
Cuts at: 1678 4191 6471 1678
Size: 2513 2280 2531
Fragments arranged by size:
2531 2513 2280
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2063 2095 4311 4343 5965
Size: 32 135 32 491 32 2216 32 1622
Cuts at: 5965 5997 6039 6071 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2216 1622 491 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2403 4417 4668 4914 669
Size: 988 281 465 2014 251 246 3079
Fragments arranged by size:
3079 2014 988 465 281 251 246
HindIII A'AGCT_T
Cuts at: 748 3873 748
Size: 3125 4199
HpaI GTT'AAC
Cuts at: 4417 4914 4417
Size: 497 6827
KpnI G_GTAC'C
Cuts at: 3299 4649 3299
Size: 1350 5974
MfeI C'AATT_G
Cuts at: 4923 4923
Size: 7324
MluI A'CGCG_T
Cuts at: 7200 7200
Size: 7324
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3011 3371 6438 6622 1305
Size: 1706 360 3067 184 2007
Fragments arranged by size:
3067 2007 1706 360 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4057 4085 10
Size: 54 1425 343 2225 28 3249
Fragments arranged by size:
3249 2225 1425 343 54 28
MslI CAynn'nnrTG
Cuts at: 518 1166 2286 2558 2753 5282 5641 5800 518
Size: 648 1120 272 195 2529 359 159 2042
Fragments arranged by size:
2529 2042 1120 648 359 272 195 159
MspA1I CmG'CkG
Cuts at: 1606 4018 4740 5417 6565 6810 1606
Size: 2412 722 677 1148 245 2120
Fragments arranged by size:
2412 2120 1148 722 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5833
Fragments arranged by size:
5833 939 552
NdeI CA'TA_TG
Cuts at: 387 4428 387
Size: 4041 3283
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7324
NotI GC'GGCC_GC
Cuts at: 4771 4771
Size: 7324
NspI r_CATG'y
Cuts at: 1132 1229 3168 4027 4595 4682 6227 1132
Size: 97 1939 859 568 87 1545 2229
Fragments arranged by size:
2229 1939 1545 859 568 97 87
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7324
PciI A'CATG_T
Cuts at: 4591 6223 4591
Size: 1632 5692
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3659 1108
Size: 2551 4773
PfoI T'CCnGG_A
Cuts at: 2776 3683 2776
Size: 907 6417
PmeI GTTT'AAAC
Cuts at: 4628 4628
Size: 7324
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5393 5425 6906 6938 5393
Size: 32 1481 32 5779
Fragments arranged by size:
5779 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2096 2096
Size: 7324
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7324
PsiI TTA'TAA
Cuts at: 4894 4894
Size: 7324
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3070 3102 3070
Size: 32 7292
PstI C_TGCA'G
Cuts at: 830 3319 3736 3955 4676 830
Size: 2489 417 219 721 3478
Fragments arranged by size:
3478 2489 721 417 219
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7324
PvuII CAG'CTG
Cuts at: 4018 4018
Size: 7324
SalI G'TCGA_C
Cuts at: 1936 4666 1936
Size: 2730 4594
SbfI CC_TGCA'GG
Cuts at: 4676 4676
Size: 7324
ScaI AGT'ACT
Cuts at: 2710 2740 5572 7284 2710
Size: 30 2832 1712 2750
Fragments arranged by size:
2832 2750 1712 30
SfcI C'TryA_G
Cuts at: 826 1045 2115 2508 3240 3315 3732 3951 4672
Size: 219 1070 393 732 75 417 219 721
Cuts at: 4672 5807 6488 6679 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1135 1070 732 721 681 417 393
219 219 191 75
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7324
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7324
SmaI CCC'GGG
Cuts at: 1872 4651 1872
Size: 2779 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2069 2143 2961 3201 4028 4358
Size: 197 926 126 74 818 240 827 330
Cuts at: 4358 5428 6329 6591 820
Size: 1070 901 262 1553
Fragments arranged by size:
1553 1070 926 901 827 818 330 262
240 197 126 74
SnaBI TAC'GTA
Cuts at: 493 5115 493
Size: 4622 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7324
SphI G_CATG'C
Cuts at: 1132 4682 1132
Size: 3550 3774
SspI AAT'ATT
Cuts at: 5 52 2225 5248 5
Size: 47 2173 3023 2081
Fragments arranged by size:
3023 2173 2081 47
StuI AGG'CCT
Cuts at: 1517 3510 1517
Size: 1993 5331
StyI C'CwwG_G
Cuts at: 513 1065 2004 4763 513
Size: 552 939 2759 3074
Fragments arranged by size:
3074 2759 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2394 5351 5368 5521 5706 6151 325
Size: 1255 814 2957 17 153 185 445 1498
Fragments arranged by size:
2957 1498 1255 814 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2228 2708 2738
Size: 274 80 33 51 1443 251 480 30
Cuts at: 2738 3778 3983 5570 7282 96
Size: 1040 205 1587 1712 138
Fragments arranged by size:
1712 1587 1443 1040 480 274 251 205
138 80 51 33 30
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7284 7316 7284
Size: 32 7292
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7324
XbaI T'CTAG_A
Cuts at: 4660 4660
Size: 7324
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7324
XmaI C'CCGG_G
Cuts at: 1870 4649 1870
Size: 2779 4545
XmnI GAAnn'nnTTC
Cuts at: 2157 3068 5453 2157
Size: 911 2385 4028
Fragments arranged by size:
4028 2385 911
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5132 276
Size: 53 83 186 936 3598 2468
Fragments arranged by size:
3598 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PsrI PstI PvuI PvuII SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
BfrBI BsiWI BsmBI BstZ17I DraIII EcoICRI FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PspOMI PspXI RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI XcmI