Restriction map for
pFN21AB3232
(Circular) (Six-base) MAPSORT of: pFN21AB3232.seq Check: 2255 from: 1 to: 6844
pFN21AB3232 6844 bp
With 182 enzymes: SgfI *
February 27, 2009 14:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3012 3412 3012
Size: 400 6444
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4654 278
Size: 53 83 186 936 3118 2468
Fragments arranged by size:
3118 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2126 4187 1937
Size: 189 2061 4594
Fragments arranged by size:
4594 2061 189
Acc65I G'GTAC_C
Cuts at: 2071 3423 4165 2071
Size: 1352 742 4750
Fragments arranged by size:
4750 1352 742
AclI AA'CG_TT
Cuts at: 1563 4971 5344 1563
Size: 3408 373 3063
Fragments arranged by size:
3408 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3752 4907 6291 6559 1632
Size: 2120 1155 1384 268 1917
Fragments arranged by size:
2120 1917 1384 1155 268
AflII C'TTAA_G
Cuts at: 820 1017 2864 820
Size: 197 1847 4800
Fragments arranged by size:
4800 1847 197
AflIII A'CryG_T
Cuts at: 1203 5743 6720 1203
Size: 4540 977 1327
Fragments arranged by size:
4540 1327 977
AgeI A'CCGG_T
Cuts at: 5675 5675
Size: 6844
AhdI GACnn_n'nnGTC
Cuts at: 5573 5573
Size: 6844
AleI CACnn'nnGTG
Cuts at: 1166 3494 1166
Size: 2328 4516
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2522 2693 3347 1184
Size: 1338 171 654 4681
Fragments arranged by size:
4681 1338 654 171
AlwNI CAG_nnn'CTG
Cuts at: 2224 2362 2553 3508 3589 4587 6159 2224
Size: 138 191 955 81 998 1572 2909
Fragments arranged by size:
2909 1572 998 955 191 138 81
ApaLI G'TGCA_C
Cuts at: 4901 6057 4901
Size: 1156 5688
ApoI r'AATT_y
Cuts at: 1493 4138 4153 4379 4531 6640 1493
Size: 2645 15 226 152 2109 1697
Fragments arranged by size:
2645 2109 1697 226 152 15
AseI AT'TA_AT
Cuts at: 160 1032 5398 160
Size: 872 4366 1606
Fragments arranged by size:
4366 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6844
AvaI C'yCGr_G
Cuts at: 1870 1943 2328 2754 2786 2933 3362 3437 3732
Size: 73 385 426 32 147 429 75 295
Cuts at: 3732 4169 1870
Size: 437 4545
Fragments arranged by size:
4545 437 429 426 385 295 147 75
73 32
BamHI G'GATC_C
Cuts at: 4174 4541 4174
Size: 367 6477
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2048 2071 2758 2782 3155
Size: 325 217 627 261 23 687 24 373
Cuts at: 3155 3235 3423 4165 5620 618
Size: 80 188 742 1455 1842
Fragments arranged by size:
1842 1455 742 687 627 373 325 261
217 188 80 24 23
BanII G_rGCy'C
Cuts at: 1391 2793 3597 3897 4163 1391
Size: 1402 804 300 266 4072
Fragments arranged by size:
4072 1402 804 300 266
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5873
BbvCI CC'TCA_GC
Cuts at: 2495 2495
Size: 6844
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2897 2931 5035 5069 1903
Size: 34 960 34 2104 34 3678
Fragments arranged by size:
3678 2104 960 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4736 5946 4736
Size: 1210 5634
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6844
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2480 3908 5455
Size: 107 122 71 631 605 808 1428 1547
Cuts at: 5455 136
Size: 1525
Fragments arranged by size:
1547 1525 1428 808 631 605 122 107
71
BglII A'GATC_T
Cuts at: 2685 3366 6839 2685
Size: 681 3473 2690
Fragments arranged by size:
3473 2690 681
BlpI GC'TnA_GC
Cuts at: 2363 4261 2363
Size: 1898 4946
Bme1580I G_kGCm'C
Cuts at: 1790 2067 2763 2773 2787 3158 3240 3346 4033
Size: 277 696 10 14 371 82 106 687
Cuts at: 4033 4905 6061 1790
Size: 872 1156 2573
Fragments arranged by size:
2573 1156 872 696 687 371 277 106
82 14 10
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6844
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3382 3793 3817
Size: 416 53 83 399 24 1965 411 24
Cuts at: 3817 4575 5533 442
Size: 758 958 1753
Fragments arranged by size:
1965 1753 958 758 416 411 399 83
53 24 24
BmtI G_CTAG'C
Cuts at: 1056 2036 2666 3944 1056
Size: 980 630 1278 3956
Fragments arranged by size:
3956 1278 980 630
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2746 2917 4011 2746
Size: 171 1094 5579
Fragments arranged by size:
5579 1094 171
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2420 2723 2741 2807 3197 3287 3366 3470
Size: 1181 303 18 66 390 90 79 104
Cuts at: 3470 3545 3611 3987 4045 5504 1239
Size: 75 66 376 58 1459 2579
Fragments arranged by size:
2579 1459 1181 390 376 303 104 90
79 75 66 66 58 18
Bpu10I CC'TnA_GC
Cuts at: 2495 2495
Size: 6844
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2777 4969 5834 6132 2777
Size: 2192 865 298 3489
Fragments arranged by size:
3489 2192 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2905 3011 3104 3602 5507 882
Size: 617 1406 106 93 498 1905 2219
Fragments arranged by size:
2219 1905 1406 617 498 106 93
BsaAI yAC'GTr
Cuts at: 493 1204 4635 493
Size: 711 3431 2702
Fragments arranged by size:
3431 2702 711
BsaBI GATnn'nnATC
Cuts at: 6838 6838
Size: 6844
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4651 5033 5668 275
Size: 53 83 186 936 3118 382 635 1451
Fragments arranged by size:
3118 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3205 5277 5675 5949 6096 6507 3205
Size: 2072 398 274 147 411 3542
Fragments arranged by size:
3542 2072 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2507 2537 4103 4133 1178
Size: 30 165 30 1104 30 1566 30 3889
Fragments arranged by size:
3889 1566 1104 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2393 2906 3548 3679 1186
Size: 1207 513 642 131 4351
Fragments arranged by size:
4351 1207 642 513 131
BseYI C'CCAG_C
Cuts at: 2269 2355 2872 3138 3386 3935 6047 6543 2269
Size: 86 517 266 248 549 2112 496 2570
Fragments arranged by size:
2570 2112 549 517 496 266 248 86
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2354 2498 2843 3623 1163
Size: 573 618 144 345 780 4384
Fragments arranged by size:
4384 780 618 573 345 144
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4294 5055 6083 1540
Size: 275 186 2293 761 1028 2301
Fragments arranged by size:
2301 2293 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3556 3597 4905 4990 6061 3556
Size: 41 1308 85 1071 4339
Fragments arranged by size:
4339 1308 1071 85 41
BsmI GAATG_Cn'
Cuts at: 4355 4448 4355
Size: 93 6751
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2067 2763 2773 2787 2793 3158 3240
Size: 399 277 696 10 14 6 365 82
Cuts at: 3240 3346 3556 3597 3897 4033 4163 4905 4990
Size: 106 210 41 300 136 130 742 85
Cuts at: 4990 6061 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 696 399 365 300 277
210 136 130 106 85 82 41 14
10 6
BspEI T'CCGG_A
Cuts at: 3205 6507 3205
Size: 3302 3542
BspHI T'CATG_A
Cuts at: 2586 4733 6463 2586
Size: 2147 1730 2967
Fragments arranged by size:
2967 2147 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2655 2685 3012 3093 3412 4199 844
Size: 1811 30 327 81 319 787 3489
Fragments arranged by size:
3489 1811 787 327 319 81 30
BsrBI CCG'CTC
Cuts at: 1394 1823 2136 2821 4731 1394
Size: 429 313 685 1910 3507
Fragments arranged by size:
3507 1910 685 429 313
BsrDI GCAATG_nn'
Cuts at: 65 1248 2176 3334 3897 5339 5513 65
Size: 1183 928 1158 563 1442 174 1396
Fragments arranged by size:
1442 1396 1183 1158 928 563 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5488 5675 1700
Size: 110 3678 187 2869
Fragments arranged by size:
3678 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6844
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6844
BssSI C'ACGA_G
Cuts at: 2015 4904 5916 2015
Size: 2889 1012 2943
Fragments arranged by size:
2943 2889 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6844
BstBI TT'CG_AA
Cuts at: 5648 5648
Size: 6844
BstEII G'GTnAC_C
Cuts at: 1184 3640 1184
Size: 2456 4388
BstXI CCAn_nnnn'nTGG
Cuts at: 3718 3718
Size: 6844
BstYI r'GATC_y
Cuts at: 1972 2112 2685 3366 4097 4174 4541 4926 4943
Size: 140 573 681 731 77 367 385 17
Cuts at: 4943 6395 6481 6493 6839 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 731 681 573 385 367 346
140 86 77 17 12
BstZ17I GTA'TAC
Cuts at: 2127 2127
Size: 6844
Bsu36I CC'TnA_GG
Cuts at: 2534 3877 2534
Size: 1343 5501
BtgI C'CryG_G
Cuts at: 513 1065 2067 2966 3103 3150 3774 513
Size: 552 1002 899 137 47 624 3583
Fragments arranged by size:
3583 1002 899 624 552 137 47
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2455 487
Size: 683 56 1229 4876
Fragments arranged by size:
4876 1229 683 56
BtsI GCAGTG_nn'
Cuts at: 2052 4360 5154 5174 2052
Size: 2308 794 20 3722
Fragments arranged by size:
3722 2308 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6844
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6809
DraI TTT'AAA
Cuts at: 4148 4148
Size: 6844
DraIII CAC_nnn'GTG
Cuts at: 3192 3192
Size: 6844
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3070 5851 809
Size: 507 1754 2781 1802
Fragments arranged by size:
2781 1802 1754 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2280 2424 2640
Size: 54 1425 50 275 18 450 144 216
Cuts at: 2640 3348 4291 5180 6809 8
Size: 708 943 889 1629 43
Fragments arranged by size:
1629 1425 943 889 708 450 275 216
144 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4291 1537
Size: 275 2479 4090
Fragments arranged by size:
4090 2479 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3468 4774 1353
Size: 2115 1306 3423
Fragments arranged by size:
3423 2115 1306
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5431 5805 5951 387
Size: 1064 138 3842 374 146 1280
Fragments arranged by size:
3842 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3595 3595
Size: 6844
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2420 2723 2741 2807 3197 3287 3366
Size: 393 788 303 18 66 390 90 79
Cuts at: 3366 3470 3545 3611 3752 3987 4045 4907 5504
Size: 104 75 66 141 235 58 862 597
Cuts at: 5504 6291 6559 1239
Size: 787 268 1524
Fragments arranged by size:
1524 862 788 787 597 393 390 303
268 235 141 104 90 79 75 66
66 58 18
EcoNI CCTnn'n_nnAGG
Cuts at: 3089 3767 3089
Size: 678 6166
EcoO109I rG'GnC_Cy
Cuts at: 2481 3444 2481
Size: 963 5881
EcoRI G'AATT_C
Cuts at: 4153 4531 4153
Size: 378 6466
FspI TGC'GCA
Cuts at: 5350 5350
Size: 6844
HaeII r_GCGC'y
Cuts at: 1678 3220 5991 1678
Size: 1542 2771 2531
Fragments arranged by size:
2771 2531 1542
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3167 3199 5485 5517 5559
Size: 32 135 32 1595 32 2286 32 42
Cuts at: 5559 5591 1373
Size: 32 2626
Fragments arranged by size:
2626 2286 1595 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4188 4434 669
Size: 988 281 2250 246 3079
Fragments arranged by size:
3079 2250 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3286 748
Size: 2538 4306
HpaI GTT'AAC
Cuts at: 4434 4434
Size: 6844
KpnI G_GTAC'C
Cuts at: 2075 3427 4169 2075
Size: 1352 742 4750
Fragments arranged by size:
4750 1352 742
MfeI C'AATT_G
Cuts at: 4443 4443
Size: 6844
MluI A'CGCG_T
Cuts at: 6720 6720
Size: 6844
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2550 2715 5958 6142 1305
Size: 1245 165 3243 184 2007
Fragments arranged by size:
3243 2007 1245 184 165
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2282 2426 2642 10
Size: 54 1425 343 450 144 216 4212
Fragments arranged by size:
4212 1425 450 343 216 144 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3494 4802 5161 5320 518
Size: 648 2328 1308 359 159 2042
Fragments arranged by size:
2328 2042 1308 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2359 2389 2553 2749 2827 3120 3138 3386
Size: 753 30 164 196 78 293 18 248
Cuts at: 3386 3935 4260 4937 6085 6330 1606
Size: 549 325 677 1148 245 2120
Fragments arranged by size:
2120 1148 753 677 549 325 293 248
245 196 164 78 30 18
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6292
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6844
NheI G'CTAG_C
Cuts at: 1052 2032 2662 3940 1052
Size: 980 630 1278 3956
Fragments arranged by size:
3956 1278 980 630
NotI GC'GGCC_GC
Cuts at: 4291 4291
Size: 6844
NspI r_CATG'y
Cuts at: 1132 1229 2084 2857 4202 5747 1132
Size: 97 855 773 1345 1545 2229
Fragments arranged by size:
2229 1545 1345 855 773 97
PasI CC'CwG_GG
Cuts at: 1348 2478 1348
Size: 1130 5714
PciI A'CATG_T
Cuts at: 5743 5743
Size: 6844
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3508 1108
Size: 2400 4444
PfoI T'CCnGG_A
Cuts at: 2616 3243 2616
Size: 627 6217
PmeI GTTT'AAAC
Cuts at: 4148 4148
Size: 6844
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2430 2462 4913 4945 6426 6458 2430
Size: 32 2451 32 1481 32 2816
Fragments arranged by size:
2816 2451 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3444 3444
Size: 6844
PshAI GACnn'nnGTC
Cuts at: 1613 2189 1613
Size: 576 6268
PsiI TTA'TAA
Cuts at: 4414 4414
Size: 6844
PstI C_TGCA'G
Cuts at: 830 2235 3090 4196 830
Size: 1405 855 1106 3478
Fragments arranged by size:
3478 1405 1106 855
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6844
PvuII CAG'CTG
Cuts at: 2359 2389 2749 3386 3935 2359
Size: 30 360 637 549 5268
Fragments arranged by size:
5268 637 549 360 30
RsrII CG'GwC_CG
Cuts at: 3226 3226
Size: 6844
SacI G_AGCT'C
Cuts at: 3597 3597
Size: 6844
SalI G'TCGA_C
Cuts at: 1936 4186 1936
Size: 2250 4594
SanDI GG'GwC_CC
Cuts at: 3444 3444
Size: 6844
SbfI CC_TGCA'GG
Cuts at: 3090 4196 3090
Size: 1106 5738
ScaI AGT'ACT
Cuts at: 3782 5092 6804 3782
Size: 1310 1712 3822
Fragments arranged by size:
3822 1712 1310
SexAI A'CCwGG_T
Cuts at: 3507 3674 3507
Size: 167 6677
SfcI C'TryA_G
Cuts at: 826 1045 2231 2432 3086 4192 5327 6008 6199
Size: 219 1186 201 654 1106 1135 681 191
Cuts at: 6199 826
Size: 1471
Fragments arranged by size:
1471 1186 1135 1106 681 654 219 201
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6844
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6844
SmaI CCC'GGG
Cuts at: 1872 2756 2788 4171 1872
Size: 884 32 1383 4545
Fragments arranged by size:
4545 1383 884 32
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2792 2864 4948 5849 6111 820
Size: 197 926 849 72 2084 901 262 1553
Fragments arranged by size:
2084 1553 926 901 849 262 197 72
SnaBI TAC'GTA
Cuts at: 493 4635 493
Size: 4142 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6844
SphI G_CATG'C
Cuts at: 1132 4202 1132
Size: 3070 3774
SrfI GCCC'GGGC
Cuts at: 2788 2788
Size: 6844
SspI AAT'ATT
Cuts at: 5 52 4768 5
Size: 47 4716 2081
Fragments arranged by size:
4716 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6844
StyI C'CwwG_G
Cuts at: 513 1065 3952 4283 513
Size: 552 2887 331 3074
Fragments arranged by size:
3074 2887 552 331
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2029 3664 3836 3971 4871 4888 5041
Size: 1255 449 1635 172 135 900 17 153
Cuts at: 5041 5226 5671 325
Size: 185 445 1498
Fragments arranged by size:
1635 1498 1255 900 449 445 185 172
153 135 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3780 5090 6802
Size: 274 80 33 51 1443 1803 1310 1712
Cuts at: 6802 96
Size: 138
Fragments arranged by size:
1803 1712 1443 1310 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2507 2539 3273 3305 6804 6836 2507
Size: 32 734 32 3499 32 2515
Fragments arranged by size:
3499 2515 734 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3128 1316
Size: 1812 5032
XbaI T'CTAG_A
Cuts at: 4180 4180
Size: 6844
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2065 2770 3311 3976 2065
Size: 705 541 665 4933
Fragments arranged by size:
4933 705 665 541
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6844
XmaI C'CCGG_G
Cuts at: 1870 2754 2786 4169 1870
Size: 884 32 1383 4545
Fragments arranged by size:
4545 1383 884 32
XmnI GAAnn'nnTTC
Cuts at: 4973 4973
Size: 6844
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4652 276
Size: 53 83 186 936 3118 2468
Fragments arranged by size:
3118 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII RsrII SacI SalI SanDI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BsiWI BsmBI EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI
PsrI SacII SapI SfiI SfoI SwaI