Restriction map for
pFN21AB3224
(Circular) (Six-base) MAPSORT of: pFN21AB3224.seq Check: 2568 from: 1 to: 6946
pFN21AB3224 6946 bp
With 182 enzymes: SgfI *
February 27, 2009 14:34 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4756 278
Size: 53 83 186 936 3220 2468
Fragments arranged by size:
3220 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2207 4289 1937
Size: 270 2082 4594
Fragments arranged by size:
4594 2082 270
Acc65I G'GTAC_C
Cuts at: 4267 4267
Size: 6946
AclI AA'CG_TT
Cuts at: 1563 5073 5446 1563
Size: 3510 373 3063
Fragments arranged by size:
3510 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2438 2499 2528 2706 2853 3045 3876 4143
Size: 806 61 29 178 147 192 831 267
Cuts at: 4143 5009 6393 6661 1632
Size: 866 1384 268 1917
Fragments arranged by size:
1917 1384 866 831 806 268 267 192
178 147 61 29
AflII C'TTAA_G
Cuts at: 820 1017 3134 820
Size: 197 2117 4632
Fragments arranged by size:
4632 2117 197
AflIII A'CryG_T
Cuts at: 1203 3059 5845 6822 1203
Size: 1856 2786 977 1327
Fragments arranged by size:
2786 1856 1327 977
AgeI A'CCGG_T
Cuts at: 5777 5777
Size: 6946
AhdI GACnn_n'nnGTC
Cuts at: 3220 5675 3220
Size: 2455 4491
AleI CACnn'nnGTG
Cuts at: 1166 2078 1166
Size: 912 6034
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3009 1184
Size: 1825 5121
AlwNI CAG_nnn'CTG
Cuts at: 2103 2336 3002 4689 6261 2103
Size: 233 666 1687 1572 2788
Fragments arranged by size:
2788 1687 1572 666 233
ApaLI G'TGCA_C
Cuts at: 5003 6159 5003
Size: 1156 5790
ApoI r'AATT_y
Cuts at: 1493 4255 4481 4633 6742 1493
Size: 2762 226 152 2109 1697
Fragments arranged by size:
2762 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5500 160
Size: 872 4468 1606
Fragments arranged by size:
4468 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6946
AvaI C'yCGr_G
Cuts at: 1870 1943 4271 1870
Size: 73 2328 4545
Fragments arranged by size:
4545 2328 73
BamHI G'GATC_C
Cuts at: 4012 4276 4643 4012
Size: 264 367 6315
Fragments arranged by size:
6315 367 264
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4267 5722 618
Size: 325 217 627 2480 1455 1842
Fragments arranged by size:
2480 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2861 4265 1391
Size: 1470 1404 4072
Fragments arranged by size:
4072 1470 1404
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5975
BbvCI CC'TCA_GC
Cuts at: 3267 3267
Size: 6946
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2703 2737 5137 5171 1903
Size: 34 50 34 682 34 2400 34 3678
Fragments arranged by size:
3678 2400 682 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3049 3671 4838 6048 3049
Size: 622 1167 1210 3947
Fragments arranged by size:
3947 1210 1167 622
BclI T'GATC_A
Cuts at: 1548 2170 2953 1548
Size: 622 783 5541
Fragments arranged by size:
5541 783 622
BfrBI ATG'CAT
Cuts at: 2843 2843
Size: 6946
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2142 5557 136
Size: 107 122 71 631 605 470 3415 1525
Fragments arranged by size:
3415 1525 631 605 470 122 107 71
BglII A'GATC_T
Cuts at: 6941 6941
Size: 6946
BlpI GC'TnA_GC
Cuts at: 3522 4363 3522
Size: 841 6105
Bme1580I G_kGCm'C
Cuts at: 1790 5007 6163 1790
Size: 3217 1156 2573
Fragments arranged by size:
3217 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6946
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2232 2343 2449
Size: 416 53 83 399 24 815 111 106
Cuts at: 2449 3761 4677 5635 442
Size: 1312 916 958 1753
Fragments arranged by size:
1753 1312 958 916 815 416 399 111
106 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6946
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2081 2628 2813 3528 4022 5606 1239
Size: 842 547 185 715 494 1584 2579
Fragments arranged by size:
2579 1584 842 715 547 494 185
Bpu10I CC'TnA_GC
Cuts at: 3267 3267
Size: 6946
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3941 5071 5936 6234 3941
Size: 1130 865 298 4653
Fragments arranged by size:
4653 1130 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2557 3988 5609 882
Size: 617 1058 1431 1621 2219
Fragments arranged by size:
2219 1621 1431 1058 617
BsaAI yAC'GTr
Cuts at: 493 1204 4737 493
Size: 711 3533 2702
Fragments arranged by size:
3533 2702 711
BsaBI GATnn'nnATC
Cuts at: 6940 6940
Size: 6946
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4753 5135 5770 275
Size: 53 83 186 936 3220 382 635 1451
Fragments arranged by size:
3220 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5379 5777 6051 6198 6609 5379
Size: 398 274 147 411 5716
Fragments arranged by size:
5716 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2554 2584 1178
Size: 30 165 30 1151 30 5540
Fragments arranged by size:
5540 1151 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2493 2575 1186
Size: 1307 82 5557
Fragments arranged by size:
5557 1307 82
BseYI C'CCAG_C
Cuts at: 2261 3139 4191 6149 6645 2261
Size: 878 1052 1958 496 2562
Fragments arranged by size:
2562 1958 1052 878 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6373
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4396 5157 6185 1540
Size: 275 186 2395 761 1028 2301
Fragments arranged by size:
2395 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 5007 5092 6163 5007
Size: 85 1071 5790
Fragments arranged by size:
5790 1071 85
BsmI GAATG_Cn'
Cuts at: 2726 4457 4550 2726
Size: 1731 93 5122
Fragments arranged by size:
5122 1731 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2861 4265 5007 5092 6163 1391
Size: 399 1071 1404 742 85 1071 2174
Fragments arranged by size:
2174 1404 1071 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 6609 6609
Size: 6946
BspHI T'CATG_A
Cuts at: 3313 4835 6565 3313
Size: 1522 1730 3694
Fragments arranged by size:
3694 1730 1522
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3517 4301 844
Size: 2673 784 3489
Fragments arranged by size:
3489 2673 784
BsrBI CCG'CTC
Cuts at: 1394 1823 4833 1394
Size: 429 3010 3507
Fragments arranged by size:
3507 3010 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2861 3333 3943 5441 5615 65
Size: 1183 1613 472 610 1498 174 1396
Fragments arranged by size:
1613 1498 1396 1183 610 472 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2965 5590 5777 1700
Size: 110 1155 2625 187 2869
Fragments arranged by size:
2869 2625 1155 187 110
BsrGI T'GTAC_A
Cuts at: 96 2462 3569 96
Size: 2366 1107 3473
Fragments arranged by size:
3473 2366 1107
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6946
BssSI C'ACGA_G
Cuts at: 5006 6018 5006
Size: 1012 5934
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6946
BstBI TT'CG_AA
Cuts at: 5750 5750
Size: 6946
BstEII G'GTnAC_C
Cuts at: 1184 2080 1184
Size: 896 6050
BstXI CCAn_nnnn'nTGG
Cuts at: 3883 3883
Size: 6946
BstYI r'GATC_y
Cuts at: 1972 2761 4012 4276 4643 5028 5045 6497 6583
Size: 789 1251 264 367 385 17 1452 86
Cuts at: 6583 6595 6941 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1251 789 385 367 346 264
86 17 12
BstZ17I GTA'TAC
Cuts at: 2208 2208
Size: 6946
BtgI C'CryG_G
Cuts at: 513 1065 2137 2787 513
Size: 552 1072 650 4672
Fragments arranged by size:
4672 1072 650 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6207
Fragments arranged by size:
6207 683 56
BtsI GCAGTG_nn'
Cuts at: 2152 3981 4462 5256 5276 2152
Size: 1829 481 794 20 3822
Fragments arranged by size:
3822 1829 794 481 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6946
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3936 3971 529
Size: 35 3372 35 3504
Fragments arranged by size:
3504 3372 35 35
DraI TTT'AAA
Cuts at: 2250 4250 2250
Size: 2000 4946
DraIII CAC_nnn'GTG
Cuts at: 2226 2351 2226
Size: 125 6821
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5953 809
Size: 507 4637 1802
Fragments arranged by size:
4637 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2737 4393 5282
Size: 54 1425 50 275 18 907 1656 889
Cuts at: 5282 6911 8
Size: 1629 43
Fragments arranged by size:
1656 1629 1425 907 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4393 1537
Size: 275 2581 4090
Fragments arranged by size:
4090 2581 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2976 3681 4876 1353
Size: 1623 705 1195 3423
Fragments arranged by size:
3423 1623 1195 705
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5533 5907 6053 387
Size: 1064 138 3944 374 146 1280
Fragments arranged by size:
3944 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2081 2438 2499 2528 2628 2706 2813
Size: 393 449 357 61 29 100 78 107
Cuts at: 2813 2853 3045 3528 3876 4022 4143 5009 5606
Size: 40 192 483 348 146 121 866 597
Cuts at: 5606 6393 6661 1239
Size: 787 268 1524
Fragments arranged by size:
1524 866 787 597 483 449 393 357
348 268 192 146 121 107 100 78
61 40 29
EcoRI G'AATT_C
Cuts at: 4255 4633 4255
Size: 378 6568
EcoRV GAT'ATC
Cuts at: 2904 2904
Size: 6946
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3211 3680 3211
Size: 469 6477
FspI TGC'GCA
Cuts at: 3597 5452 3597
Size: 1855 5091
FspAI rTGC'GCAy
Cuts at: 3597 3597
Size: 6946
HaeII r_GCGC'y
Cuts at: 1678 6093 1678
Size: 4415 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2100 2132 2554 2586 3206
Size: 32 135 32 528 32 422 32 620
Cuts at: 3206 3238 3395 3427 3645 3677 5587 5619 5661
Size: 32 157 32 218 32 1910 32 42
Cuts at: 5661 5693 1373
Size: 32 2626
Fragments arranged by size:
2626 1910 620 528 422 218 157 135
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3772 4290 4536 669
Size: 988 281 1834 518 246 3079
Fragments arranged by size:
3079 1834 988 518 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6946
HpaI GTT'AAC
Cuts at: 4536 4536
Size: 6946
KpnI G_GTAC'C
Cuts at: 4271 4271
Size: 6946
MfeI C'AATT_G
Cuts at: 4545 4545
Size: 6946
MluI A'CGCG_T
Cuts at: 6822 6822
Size: 6946
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2250 2578 3631 3654 4250 6060 6244 1305
Size: 945 328 1053 23 596 1810 184 2007
Fragments arranged by size:
2007 1810 1053 945 596 328 184 23
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2739 10
Size: 54 1425 343 907 4217
Fragments arranged by size:
4217 1425 907 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2078 4904 5263 5422 518
Size: 648 912 2826 359 159 2042
Fragments arranged by size:
2826 2042 912 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2078 2103 2139 3266 3854 4362 5039 6187
Size: 472 25 36 1127 588 508 677 1148
Cuts at: 6187 6432 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 1127 677 588 508 472 245
36 25
NcoI C'CATG_G
Cuts at: 513 1065 2787 513
Size: 552 1722 4672
Fragments arranged by size:
4672 1722 552
NdeI CA'TA_TG
Cuts at: 387 3715 387
Size: 3328 3618
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6946
NotI GC'GGCC_GC
Cuts at: 4393 4393
Size: 6946
NsiI A_TGCA'T
Cuts at: 2845 2845
Size: 6946
NspI r_CATG'y
Cuts at: 1132 1229 2843 3063 3602 3631 4304 5849 1132
Size: 97 1614 220 539 29 673 1545 2229
Fragments arranged by size:
2229 1614 1545 673 539 220 97 29
PasI CC'CwG_GG
Cuts at: 1348 3439 1348
Size: 2091 4855
PciI A'CATG_T
Cuts at: 3059 5845 3059
Size: 2786 4160
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2656 3827 1108
Size: 1548 1171 4227
Fragments arranged by size:
4227 1548 1171
PfoI T'CCnGG_A
Cuts at: 3383 3383
Size: 6946
PmeI GTTT'AAAC
Cuts at: 4250 4250
Size: 6946
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5015 5047 6528 6560 5015
Size: 32 1481 32 5401
Fragments arranged by size:
5401 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6946
PsiI TTA'TAA
Cuts at: 4516 4516
Size: 6946
PstI C_TGCA'G
Cuts at: 830 4298 830
Size: 3468 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6946
PvuII CAG'CTG
Cuts at: 2103 3266 3854 2103
Size: 1163 588 5195
Fragments arranged by size:
5195 1163 588
SacII CC_GC'GG
Cuts at: 2140 2140
Size: 6946
SalI G'TCGA_C
Cuts at: 1936 4288 1936
Size: 2352 4594
SapI GCTCTTCn'nnn_
Cuts at: 2976 2976
Size: 6946
SbfI CC_TGCA'GG
Cuts at: 4298 4298
Size: 6946
ScaI AGT'ACT
Cuts at: 2276 3296 5194 6906 2276
Size: 1020 1898 1712 2316
Fragments arranged by size:
2316 1898 1712 1020
SfcI C'TryA_G
Cuts at: 826 1045 2415 3090 4294 5429 6110 6301 826
Size: 219 1370 675 1204 1135 681 191 1471
Fragments arranged by size:
1471 1370 1204 1135 681 675 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6946
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6946
SmaI CCC'GGG
Cuts at: 1872 4273 1872
Size: 2401 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3134 3920 5050 5951 6213 820
Size: 197 926 1191 786 1130 901 262 1553
Fragments arranged by size:
1553 1191 1130 926 901 786 262 197
SnaBI TAC'GTA
Cuts at: 493 4737 493
Size: 4244 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6946
SphI G_CATG'C
Cuts at: 1132 2843 4304 1132
Size: 1711 1461 3774
Fragments arranged by size:
3774 1711 1461
SspI AAT'ATT
Cuts at: 5 52 4169 4870 5
Size: 47 4117 701 2081
Fragments arranged by size:
4117 2081 701 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6946
StyI C'CwwG_G
Cuts at: 513 1065 2751 2787 3740 4154 4385 513
Size: 552 1686 36 953 414 231 3074
Fragments arranged by size:
3074 1686 953 552 414 231 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2214 2362 3452 3711 3743 4973 4990
Size: 1255 634 148 1090 259 32 1230 17
Cuts at: 4990 5143 5328 5773 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1255 1230 1090 634 445 259 185
153 148 32 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2163 2232 2274
Size: 274 80 33 51 1443 186 69 42
Cuts at: 2274 2462 3294 3569 3690 5192 6904 96
Size: 188 832 275 121 1502 1712 138
Fragments arranged by size:
1712 1502 1443 832 275 274 188 186
138 121 80 69 51 42 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3571 3603 6906 6938 3571
Size: 32 3303 32 3579
Fragments arranged by size:
3579 3303 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2568 1316
Size: 1252 5694
XbaI T'CTAG_A
Cuts at: 2294 4282 2294
Size: 1988 4958
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2924 2924
Size: 6946
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6946
XmaI C'CCGG_G
Cuts at: 1870 4271 1870
Size: 2401 4545
XmnI GAAnn'nnTTC
Cuts at: 2724 3372 5075 2724
Size: 648 1703 4595
Fragments arranged by size:
4595 1703 648
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4754 276
Size: 53 83 186 936 3220 2468
Fragments arranged by size:
3220 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoRI EcoRV FalI
FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NsiI NspI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PstI PvuI PvuII SacII
SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BplI BsiWI BsmBI Bsu36I EcoICRI EcoNI EcoO109I FseI
KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI
PspOMI PspXI PsrI RsrII SacI SanDI SexAI SfiI
SfoI SrfI SwaI