Restriction map for
pFN21AB3219
(Circular) (Six-base) MAPSORT of: pFN21AB3219.seq Check: 5827 from: 1 to: 6712
pFN21AB3219 6712 bp
With 182 enzymes: SgfI *
February 27, 2009 14:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3513 3513
Size: 6712
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4522 278
Size: 53 83 186 936 2986 2468
Fragments arranged by size:
2986 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3570 4055 1937
Size: 1633 485 4594
Fragments arranged by size:
4594 1633 485
Acc65I G'GTAC_C
Cuts at: 3890 4033 3890
Size: 143 6569
AclI AA'CG_TT
Cuts at: 1563 4839 5212 1563
Size: 3276 373 3063
Fragments arranged by size:
3276 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3326 3575 4775 6159 6427 1632
Size: 1694 249 1200 1384 268 1917
Fragments arranged by size:
1917 1694 1384 1200 268 249
AfeI AGC'GCT
Cuts at: 3227 3227
Size: 6712
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6515
AflIII A'CryG_T
Cuts at: 1203 2457 5611 6588 1203
Size: 1254 3154 977 1327
Fragments arranged by size:
3154 1327 1254 977
AgeI A'CCGG_T
Cuts at: 2190 5543 2190
Size: 3353 3359
AhdI GACnn_n'nnGTC
Cuts at: 5441 5441
Size: 6712
AleI CACnn'nnGTG
Cuts at: 1166 2021 3650 1166
Size: 855 1629 4228
Fragments arranged by size:
4228 1629 855
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3945 1184
Size: 2761 3951
AlwNI CAG_nnn'CTG
Cuts at: 2068 2608 3127 4455 6027 2068
Size: 540 519 1328 1572 2753
Fragments arranged by size:
2753 1572 1328 540 519
ApaI G_GGCC'C
Cuts at: 2959 3439 3820 3943 2959
Size: 480 381 123 5728
Fragments arranged by size:
5728 480 381 123
ApaLI G'TGCA_C
Cuts at: 2225 3532 4769 5925 2225
Size: 1307 1237 1156 3012
Fragments arranged by size:
3012 1307 1237 1156
ApoI r'AATT_y
Cuts at: 1493 4021 4247 4399 6508 1493
Size: 2528 226 152 2109 1697
Fragments arranged by size:
2528 2109 1697 226 152
AscI GG'CGCG_CC
Cuts at: 2253 2253
Size: 6712
AseI AT'TA_AT
Cuts at: 160 1032 5266 160
Size: 872 4234 1606
Fragments arranged by size:
4234 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6712
AvaI C'yCGr_G
Cuts at: 1870 1943 3211 3936 4037 1870
Size: 73 1268 725 101 4545
Fragments arranged by size:
4545 1268 725 101 73
BamHI G'GATC_C
Cuts at: 4042 4409 4042
Size: 367 6345
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2522 3194 3371 3890 4033
Size: 325 217 627 735 672 177 519 143
Cuts at: 4033 5488 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 735 672 627 519 325 217
177 143
BanII G_rGCy'C
Cuts at: 1391 2815 2959 3027 3290 3439 3459 3820 3943
Size: 1424 144 68 263 149 20 361 123
Cuts at: 3943 4031 1391
Size: 88 4072
Fragments arranged by size:
4072 1424 361 263 149 144 123 88
68 20
BbeI G_GCGC'C
Cuts at: 2526 2526
Size: 6712
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2778 3854 928
Size: 971 879 1076 3786
Fragments arranged by size:
3786 1076 971 879
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3361 3395 4903 4937 1903
Size: 34 1424 34 1508 34 3678
Fragments arranged by size:
3678 1508 1424 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4604 5814 4604
Size: 1210 5502
BclI T'GATC_A
Cuts at: 1548 3093 1548
Size: 1545 5167
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5323 136
Size: 107 122 71 631 605 3651 1525
Fragments arranged by size:
3651 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2944 3985 6707 2944
Size: 1041 2722 2949
Fragments arranged by size:
2949 2722 1041
BlpI GC'TnA_GC
Cuts at: 2072 2751 4129 2072
Size: 679 1378 4655
Fragments arranged by size:
4655 1378 679
Bme1580I G_kGCm'C
Cuts at: 1790 2229 2959 3374 3439 3536 3820 3943 4773
Size: 439 730 415 65 97 284 123 830
Cuts at: 4773 5929 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 830 730 439 415 284 123
97 65
BmgBI CAC'GTC
Cuts at: 1318 2170 2296 1318
Size: 852 126 5734
Fragments arranged by size:
5734 852 126
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3466 4443 5401
Size: 416 53 83 399 24 2049 977 958
Cuts at: 5401 442
Size: 1753
Fragments arranged by size:
2049 1753 977 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6712
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2045 2411 2453 2534 2612 2989 3104 3296
Size: 806 366 42 81 78 377 115 192
Cuts at: 3296 3539 4010 5372 1239
Size: 243 471 1362 2579
Fragments arranged by size:
2579 1362 806 471 377 366 243 192
115 81 78 42
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3566 4837 5702 6000 3566
Size: 1271 865 298 4278
Fragments arranged by size:
4278 1271 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2234 5375 882
Size: 617 735 3141 2219
Fragments arranged by size:
3141 2219 735 617
BsaAI yAC'GTr
Cuts at: 493 1204 2460 2925 3337 3691 3913 4503 493
Size: 711 1256 465 412 354 222 590 2702
Fragments arranged by size:
2702 1256 711 590 465 412 354 222
BsaBI GATnn'nnATC
Cuts at: 6706 6706
Size: 6712
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2523 3828 4519 4901
Size: 53 83 186 936 990 1305 691 382
Cuts at: 4901 5536 275
Size: 635 1451
Fragments arranged by size:
1451 1305 990 936 691 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2190 3019 5145 5543 5817 5964 6375 2190
Size: 829 2126 398 274 147 411 2527
Fragments arranged by size:
2527 2126 829 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6487
Fragments arranged by size:
6487 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2818 3092 1186
Size: 1632 274 4806
Fragments arranged by size:
4806 1632 274
BseYI C'CCAG_C
Cuts at: 2611 2914 3539 5915 6411 2611
Size: 303 625 2376 496 2912
Fragments arranged by size:
2912 2376 625 496 303
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3605 3671 3992 1163
Size: 573 1869 66 321 3883
Fragments arranged by size:
3883 1869 573 321 66
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2080 4162 4923 5951 1540
Size: 275 186 79 2082 761 1028 2301
Fragments arranged by size:
2301 2082 1028 761 275 186 79
BsiHKAI G_wGCw'C
Cuts at: 2229 2421 3536 3552 4773 4858 5929 2229
Size: 192 1115 16 1221 85 1071 3012
Fragments arranged by size:
3012 1221 1115 1071 192 85 16
BsmI GAATG_Cn'
Cuts at: 3802 4223 4316 3802
Size: 421 93 6198
Fragments arranged by size:
6198 421 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2229 2421 2815 2959 3027 3290 3374
Size: 399 439 192 394 144 68 263 84
Cuts at: 3374 3439 3459 3536 3552 3820 3943 4031 4773
Size: 65 20 77 16 268 123 88 742
Cuts at: 4773 4858 5929 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 439 399 394 268 263
192 144 123 88 85 84 77 68
65 20 16
BspEI T'CCGG_A
Cuts at: 6375 6375
Size: 6712
BspHI T'CATG_A
Cuts at: 2466 3792 4601 6331 2466
Size: 1326 809 1730 2847
Fragments arranged by size:
2847 1730 1326 809
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2517 2722 3018 3513 3522 4067 844
Size: 1673 205 296 495 9 545 3489
Fragments arranged by size:
3489 1673 545 495 296 205 9
BsrBI CCG'CTC
Cuts at: 1394 1823 4599 1394
Size: 429 2776 3507
Fragments arranged by size:
3507 2776 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5207 5381 65
Size: 1183 3959 174 1396
Fragments arranged by size:
3959 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2120 2190 2213 2282 2445 2535 5356
Size: 110 310 70 23 69 163 90 2821
Cuts at: 5356 5543 1700
Size: 187 2869
Fragments arranged by size:
2869 2821 310 187 163 110 90 70
69 23
BsrGI T'GTAC_A
Cuts at: 96 2025 96
Size: 1929 4783
BssHII G'CGCG_C
Cuts at: 1925 2253 3588 1925
Size: 328 1335 5049
Fragments arranged by size:
5049 1335 328
BssSI C'ACGA_G
Cuts at: 2473 4772 5784 2473
Size: 2299 1012 3401
Fragments arranged by size:
3401 2299 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2608 794
Size: 1814 4898
BstBI TT'CG_AA
Cuts at: 5516 5516
Size: 6712
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6712
BstXI CCAn_nnnn'nTGG
Cuts at: 2695 3487 3652 2695
Size: 792 165 5755
Fragments arranged by size:
5755 792 165
BstYI r'GATC_y
Cuts at: 1972 2595 2944 3957 3985 4042 4409 4794 4811
Size: 623 349 1013 28 57 367 385 17
Cuts at: 4811 6263 6349 6361 6707 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1013 623 385 367 349 346
86 57 28 17 12
Bsu36I CC'TnA_GG
Cuts at: 2805 2805
Size: 6712
BtgI C'CryG_G
Cuts at: 513 1065 2004 2317 2895 2979 3666 513
Size: 552 939 313 578 84 687 3559
Fragments arranged by size:
3559 939 687 578 552 313 84
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2128 487
Size: 683 56 902 5071
Fragments arranged by size:
5071 902 683 56
BtsI GCAGTG_nn'
Cuts at: 3929 4228 5022 5042 3929
Size: 299 794 20 5599
Fragments arranged by size:
5599 794 299 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6712
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6677
DraI TTT'AAA
Cuts at: 4016 4016
Size: 6712
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5719 809
Size: 507 4403 1802
Fragments arranged by size:
4403 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2077 2118 2158
Size: 54 1425 50 275 18 247 41 40
Cuts at: 2158 2284 2383 2439 2452 2667 2722 3489 3786
Size: 126 99 56 13 215 55 767 297
Cuts at: 3786 3882 4159 5048 6677 8
Size: 96 277 889 1629 43
Fragments arranged by size:
1629 1425 889 767 297 277 275 247
215 126 99 96 56 55 54 50
43 41 40 18 13
EagI C'GGCC_G
Cuts at: 1537 1812 2077 4159 1537
Size: 275 265 2082 4090
Fragments arranged by size:
4090 2082 275 265
EarI CTCTTCn'nnn_
Cuts at: 1353 3332 4642 1353
Size: 1979 1310 3423
Fragments arranged by size:
3423 1979 1310
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5299 5673 5819 387
Size: 1064 138 3710 374 146 1280
Fragments arranged by size:
3710 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2045 2411 2453 2534 2612 2989 3104
Size: 393 413 366 42 81 78 377 115
Cuts at: 3104 3296 3326 3539 3575 4010 4775 5372 6159
Size: 192 30 213 36 435 765 597 787
Cuts at: 6159 6427 1239
Size: 268 1524
Fragments arranged by size:
1524 787 765 597 435 413 393 377
366 268 213 192 115 81 78 42
36 30
EcoNI CCTnn'n_nnAGG
Cuts at: 2708 2708
Size: 6712
EcoO109I rG'GnC_Cy
Cuts at: 2322 2955 2956 3124 3436 3816 3940 2322
Size: 633 1 168 312 380 124 5094
Fragments arranged by size:
5094 633 380 312 168 124 1
EcoRI G'AATT_C
Cuts at: 4021 4399 4021
Size: 378 6334
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3625 3625
Size: 6712
FspI TGC'GCA
Cuts at: 2799 3116 5218 2799
Size: 317 2102 4293
Fragments arranged by size:
4293 2102 317
FspAI rTGC'GCAy
Cuts at: 3116 3116
Size: 6712
HaeII r_GCGC'y
Cuts at: 1678 2526 2547 3092 3229 3488 5859 1678
Size: 848 21 545 137 259 2371 2531
Fragments arranged by size:
2531 2371 848 545 259 137 21
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 5353 5385 5427 5459 1373
Size: 32 135 32 3781 32 42 32 2626
Fragments arranged by size:
3781 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3067 4056 4302 669
Size: 988 281 1129 989 246 3079
Fragments arranged by size:
3079 1129 989 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6712
HpaI GTT'AAC
Cuts at: 4302 4302
Size: 6712
KasI G'GCGC_C
Cuts at: 2522 2522
Size: 6712
KpnI G_GTAC'C
Cuts at: 3894 4037 3894
Size: 143 6569
MfeI C'AATT_G
Cuts at: 4311 4311
Size: 6712
MluI A'CGCG_T
Cuts at: 6588 6588
Size: 6712
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5826 6010 1305
Size: 4521 184 2007
Fragments arranged by size:
4521 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2441 3491 3884 10
Size: 54 1425 343 609 1050 393 2838
Fragments arranged by size:
2838 1425 1050 609 393 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2021 2232 3629 3650 4670 5029 5188
Size: 648 855 211 1397 21 1020 359 159
Cuts at: 5188 518
Size: 2042
Fragments arranged by size:
2042 1397 1020 855 648 359 211 159
21
MspA1I CmG'CkG
Cuts at: 1606 2056 2076 2263 2319 2605 2659 3034 3445
Size: 450 20 187 56 286 54 375 411
Cuts at: 3445 4128 4805 5953 6198 1606
Size: 683 677 1148 245 2120
Fragments arranged by size:
2120 1148 683 677 450 411 375 286
245 187 56 54 20
NaeI GCC'GGC
Cuts at: 2122 2284 2122
Size: 162 6550
NarI GG'CG_CC
Cuts at: 2523 2523
Size: 6712
NcoI C'CATG_G
Cuts at: 513 1065 2004 2979 513
Size: 552 939 975 4246
Fragments arranged by size:
4246 975 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6712
NgoMIV G'CCGG_C
Cuts at: 2120 2282 2120
Size: 162 6550
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6712
NotI GC'GGCC_GC
Cuts at: 4159 4159
Size: 6712
NspI r_CATG'y
Cuts at: 1132 1229 3121 3262 4070 5615 1132
Size: 97 1892 141 808 1545 2229
Fragments arranged by size:
2229 1892 1545 808 141 97
PasI CC'CwG_GG
Cuts at: 1348 3450 3594 1348
Size: 2102 144 4466
Fragments arranged by size:
4466 2102 144
PciI A'CATG_T
Cuts at: 5611 5611
Size: 6712
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6712
PfoI T'CCnGG_A
Cuts at: 2709 3135 3420 3978 2709
Size: 426 285 558 5443
Fragments arranged by size:
5443 558 426 285
PmeI GTTT'AAAC
Cuts at: 4016 4016
Size: 6712
PmlI CAC'GTG
Cuts at: 2460 3691 2460
Size: 1231 5481
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4781 4813 6294 6326 4781
Size: 32 1481 32 5167
Fragments arranged by size:
5167 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6712
PsiI TTA'TAA
Cuts at: 4282 4282
Size: 6712
PspOMI G'GGCC_C
Cuts at: 2955 3435 3816 3939 2955
Size: 480 381 123 5728
Fragments arranged by size:
5728 480 381 123
PstI C_TGCA'G
Cuts at: 830 2055 2604 2825 3444 3924 4064 830
Size: 1225 549 221 619 480 140 3478
Fragments arranged by size:
3478 1225 619 549 480 221 140
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6712
PvuII CAG'CTG
Cuts at: 2056 2605 3034 3445 2056
Size: 549 429 411 5323
Fragments arranged by size:
5323 549 429 411
SacII CC_GC'GG
Cuts at: 2320 2320
Size: 6712
SalI G'TCGA_C
Cuts at: 1936 4054 1936
Size: 2118 4594
SbfI CC_TGCA'GG
Cuts at: 4064 4064
Size: 6712
ScaI AGT'ACT
Cuts at: 4960 6672 4960
Size: 1712 5000
SexAI A'CCwGG_T
Cuts at: 2181 2406 2181
Size: 225 6487
SfcI C'TryA_G
Cuts at: 826 1045 2051 2600 2821 3440 3920 4060 5195
Size: 219 1006 549 221 619 480 140 1135
Cuts at: 5195 5876 6067 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 1006 681 619 549 480 221
219 191 140
SfoI GGC'GCC
Cuts at: 2524 2524
Size: 6712
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6712
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6712
SmaI CCC'GGG
Cuts at: 1872 3938 4039 1872
Size: 2066 101 4545
Fragments arranged by size:
4545 2066 101
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3581 4816 5717 5979 820
Size: 197 926 1638 1235 901 262 1553
Fragments arranged by size:
1638 1553 1235 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2925 4503 493
Size: 2432 1578 2702
Fragments arranged by size:
2702 2432 1578
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6712
SphI G_CATG'C
Cuts at: 1132 3121 4070 1132
Size: 1989 949 3774
Fragments arranged by size:
3774 1989 949
SrfI GCCC'GGGC
Cuts at: 3938 3938
Size: 6712
SspI AAT'ATT
Cuts at: 5 52 4636 5
Size: 47 4584 2081
Fragments arranged by size:
4584 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6712
StyI C'CwwG_G
Cuts at: 513 1065 2004 2547 2904 2979 3480 4151 513
Size: 552 939 543 357 75 501 671 3074
Fragments arranged by size:
3074 939 671 552 543 501 357 75
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3140 3389 4739 4756 4909 5094 5539
Size: 1255 1560 249 1350 17 153 185 445
Cuts at: 5539 325
Size: 1498
Fragments arranged by size:
1560 1498 1350 1255 445 249 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2025 4958 6670
Size: 274 80 33 51 1443 48 2933 1712
Cuts at: 6670 96
Size: 138
Fragments arranged by size:
2933 1712 1443 274 138 80 51 48
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3021 3053 6672 6704 3021
Size: 32 3619 32 3029
Fragments arranged by size:
3619 3029 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6712
XbaI T'CTAG_A
Cuts at: 4048 4048
Size: 6712
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3079 3364 3079
Size: 285 6427
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6712
XmaI C'CCGG_G
Cuts at: 1870 3936 4037 1870
Size: 2066 101 4545
Fragments arranged by size:
4545 2066 101
XmnI GAAnn'nnTTC
Cuts at: 3608 4841 3608
Size: 1233 5479
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4520 276
Size: 53 83 186 936 2986 2468
Fragments arranged by size:
2986 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AscI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AloI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI
BsmBI BstZ17I DraIII EcoICRI EcoRV FseI NruI NsiI
PacI PpuMI PspXI PsrI RsrII SacI SanDI SapI
SfiI SwaI