Restriction map for
pFN21AB3205
(Circular) (Six-base) MAPSORT of: pFN21AB3205.seq Check: 7243 from: 1 to: 6340
pFN21AB3205 6340 bp
With 182 enzymes: SgfI *
October 20, 2009 10:53 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4150 278
Size: 53 83 186 936 2614 2468
Fragments arranged by size:
2614 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2436 3388 3683 1937
Size: 499 952 295 4594
Fragments arranged by size:
4594 952 499 295
Acc65I G'GTAC_C
Cuts at: 3661 3661
Size: 6340
AclI AA'CG_TT
Cuts at: 1563 4467 4840 1563
Size: 2904 373 3063
Fragments arranged by size:
3063 2904 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2879 4403 5787 6055 1632
Size: 1247 1524 1384 268 1917
Fragments arranged by size:
1917 1524 1384 1247 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6143
AflIII A'CryG_T
Cuts at: 1203 2120 5239 6216 1203
Size: 917 3119 977 1327
Fragments arranged by size:
3119 1327 977 917
AgeI A'CCGG_T
Cuts at: 5171 5171
Size: 6340
AhdI GACnn_n'nnGTC
Cuts at: 5069 5069
Size: 6340
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6340
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2738 1184
Size: 1554 4786
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2052 2084 3159 3191 2052
Size: 32 1075 32 5201
Fragments arranged by size:
5201 1075 32 32
AlwNI CAG_nnn'CTG
Cuts at: 4083 5655 4083
Size: 1572 4768
ApaI G_GGCC'C
Cuts at: 2992 2992
Size: 6340
ApaLI G'TGCA_C
Cuts at: 4397 5553 4397
Size: 1156 5184
ApoI r'AATT_y
Cuts at: 1493 2802 3649 3875 4027 6136 1493
Size: 1309 847 226 152 2109 1697
Fragments arranged by size:
2109 1697 1309 847 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4894 160
Size: 872 3862 1606
Fragments arranged by size:
3862 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6340
AvaI C'yCGr_G
Cuts at: 1870 1943 3665 1870
Size: 73 1722 4545
Fragments arranged by size:
4545 1722 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3450 3483 3450
Size: 33 6307
BamHI G'GATC_C
Cuts at: 3670 4037 3670
Size: 367 5973
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3226 3661 5116 618
Size: 325 217 627 1439 435 1455 1842
Fragments arranged by size:
1842 1455 1439 627 435 325 217
BanII G_rGCy'C
Cuts at: 1391 2992 3622 3659 1391
Size: 1601 630 37 4072
Fragments arranged by size:
4072 1601 630 37
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2640 2960 928
Size: 971 741 320 4308
Fragments arranged by size:
4308 971 741 320
BbvCI CC'TCA_GC
Cuts at: 3219 3219
Size: 6340
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3179 3213 4531 4565 1903
Size: 34 1242 34 1318 34 3678
Fragments arranged by size:
3678 1318 1242 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2438 4232 5442 2438
Size: 1794 1210 3336
Fragments arranged by size:
3336 1794 1210
BclI T'GATC_A
Cuts at: 1548 2370 2403 1548
Size: 822 33 5485
Fragments arranged by size:
5485 822 33
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2987 3612 4951
Size: 107 122 71 631 605 1315 625 1339
Cuts at: 4951 136
Size: 1525
Fragments arranged by size:
1525 1339 1315 631 625 605 122 107
71
BglII A'GATC_T
Cuts at: 6335 6335
Size: 6340
BlpI GC'TnA_GC
Cuts at: 2033 2331 2904 3757 2033
Size: 298 573 853 4616
Fragments arranged by size:
4616 853 573 298
Bme1580I G_kGCm'C
Cuts at: 1790 2992 4401 5557 1790
Size: 1202 1409 1156 2573
Fragments arranged by size:
2573 1409 1202 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6340
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2426 3491 4071
Size: 416 53 83 399 24 1009 1065 580
Cuts at: 4071 5029 442
Size: 958 1753
Fragments arranged by size:
1753 1065 1009 958 580 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 3144 1056
Size: 2088 4252
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3172 3172
Size: 6340
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2363 2640 2792 3161 5000 1239
Size: 1124 277 152 369 1839 2579
Fragments arranged by size:
2579 1839 1124 369 277 152
Bpu10I CC'TnA_GC
Cuts at: 3219 3219
Size: 6340
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4465 5330 5628 4465
Size: 865 298 5177
Fragments arranged by size:
5177 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3425 5003 882
Size: 617 1926 1578 2219
Fragments arranged by size:
2219 1926 1578 617
BsaAI yAC'GTr
Cuts at: 493 1204 2687 4131 493
Size: 711 1483 1444 2702
Fragments arranged by size:
2702 1483 1444 711
BsaBI GATnn'nnATC
Cuts at: 6334 6334
Size: 6340
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4147 4529 5164 275
Size: 53 83 186 936 2614 382 635 1451
Fragments arranged by size:
2614 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2057 2949 4773 5171 5445 5592 6003 2057
Size: 892 1824 398 274 147 411 2394
Fragments arranged by size:
2394 1824 892 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2160 2190 2807 2837 3159
Size: 30 165 30 757 30 617 30 322
Cuts at: 3159 3189 1178
Size: 30 4329
Fragments arranged by size:
4329 757 617 322 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2576 2801 3119 1186
Size: 1390 225 318 4407
Fragments arranged by size:
4407 1390 318 225
BseYI C'CCAG_C
Cuts at: 5543 6039 5543
Size: 496 5844
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3103 1163
Size: 573 1367 4400
Fragments arranged by size:
4400 1367 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3790 4551 5579 1540
Size: 275 186 1789 761 1028 2301
Fragments arranged by size:
2301 1789 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2028 2034 2337 4401 4486 5557 2028
Size: 6 303 2064 85 1071 2811
Fragments arranged by size:
2811 2064 1071 303 85 6
BsmI GAATG_Cn'
Cuts at: 3851 3944 3851
Size: 93 6247
BsmBI CGTCTCn'nnnn_
Cuts at: 3446 3530 3446
Size: 84 6256
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2028 2034 2337 2992 3622 3659 4401
Size: 399 238 6 303 655 630 37 742
Cuts at: 4401 4486 5557 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 655 630 399 303 238
85 37 6
BspEI T'CCGG_A
Cuts at: 2057 2949 6003 2057
Size: 892 3054 2394
Fragments arranged by size:
3054 2394 892
BspHI T'CATG_A
Cuts at: 2658 4229 5959 2658
Size: 1571 1730 3039
Fragments arranged by size:
3039 1730 1571
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3695 844
Size: 2851 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4227 1394
Size: 429 2404 3507
Fragments arranged by size:
3507 2404 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2200 2526 2970 3208 4835 5009 65
Size: 1183 952 326 444 238 1627 174 1396
Fragments arranged by size:
1627 1396 1183 952 444 326 238 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3606 4984 5171 1700
Size: 110 1796 1378 187 2869
Fragments arranged by size:
2869 1796 1378 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6340
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6340
BssSI C'ACGA_G
Cuts at: 2027 2781 4400 5412 2027
Size: 754 1619 1012 2955
Fragments arranged by size:
2955 1619 1012 754
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2353 794
Size: 1559 4781
BstBI TT'CG_AA
Cuts at: 5144 5144
Size: 6340
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6340
BstXI CCAn_nnnn'nTGG
Cuts at: 3368 3368
Size: 6340
BstYI r'GATC_y
Cuts at: 1972 3670 4037 4422 4439 5891 5977 5989 6335
Size: 1698 367 385 17 1452 86 12 346
Cuts at: 6335 1972
Size: 1977
Fragments arranged by size:
1977 1698 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5788
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3609 487
Size: 683 56 2383 3218
Fragments arranged by size:
3218 2383 683 56
BtsI GCAGTG_nn'
Cuts at: 3537 3856 4650 4670 3537
Size: 319 794 20 5207
Fragments arranged by size:
5207 794 319 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6340
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6305
DraI TTT'AAA
Cuts at: 3644 3644
Size: 6340
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2642 5347 809
Size: 507 1326 2705 1802
Fragments arranged by size:
2705 1802 1326 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2232 3308 3631
Size: 54 1425 50 275 18 402 1076 323
Cuts at: 3631 3787 4676 6305 8
Size: 156 889 1629 43
Fragments arranged by size:
1629 1425 1076 889 402 323 275 156
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3787 1537
Size: 275 1975 4090
Fragments arranged by size:
4090 1975 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2265 2814 4270 1353
Size: 912 549 1456 3423
Fragments arranged by size:
3423 1456 912 549
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4927 5301 5447 387
Size: 1064 138 3338 374 146 1280
Fragments arranged by size:
3338 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2363 2640 2792 2879 3161 4403 5000
Size: 393 731 277 152 87 282 1242 597
Cuts at: 5000 5787 6055 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1242 787 731 597 393 282 277
268 152 87
EcoO109I rG'GnC_Cy
Cuts at: 2129 2988 3240 3331 2129
Size: 859 252 91 5138
Fragments arranged by size:
5138 859 252 91
EcoRI G'AATT_C
Cuts at: 2802 3649 4027 2802
Size: 847 378 5115
Fragments arranged by size:
5115 847 378
EcoRV GAT'ATC
Cuts at: 2110 2110
Size: 6340
FspI TGC'GCA
Cuts at: 4846 4846
Size: 6340
HaeII r_GCGC'y
Cuts at: 1678 2698 5487 1678
Size: 1020 2789 2531
Fragments arranged by size:
2789 2531 1020
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 1999 2031 4981 5013 5055
Size: 32 135 32 427 32 2950 32 42
Cuts at: 5055 5087 1373
Size: 32 2626
Fragments arranged by size:
2950 2626 427 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2437 3684 3930 669
Size: 988 281 499 1247 246 3079
Fragments arranged by size:
3079 1247 988 499 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6340
HpaI GTT'AAC
Cuts at: 3930 3930
Size: 6340
KpnI G_GTAC'C
Cuts at: 3665 3665
Size: 6340
MfeI C'AATT_G
Cuts at: 3939 3939
Size: 6340
MluI A'CGCG_T
Cuts at: 6216 6216
Size: 6340
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2257 2552 2878 3013 3033 5454 5638 1305
Size: 952 295 326 135 20 2421 184 2007
Fragments arranged by size:
2421 2007 952 326 295 184 135 20
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3310 3633 10
Size: 54 1425 343 1478 323 2717
Fragments arranged by size:
2717 1478 1425 343 323 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2177 3191 4298 4657 4816 518
Size: 648 1011 1014 1107 359 159 2042
Fragments arranged by size:
2042 1107 1014 1011 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2231 3223 3756 4433 5581 5826 1606
Size: 625 992 533 677 1148 245 2120
Fragments arranged by size:
2120 1148 992 677 625 533 245
NaeI GCC'GGC
Cuts at: 3608 3608
Size: 6340
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5788
NdeI CA'TA_TG
Cuts at: 387 3488 387
Size: 3101 3239
NgoMIV G'CCGG_C
Cuts at: 3606 3606
Size: 6340
NheI G'CTAG_C
Cuts at: 1052 3140 1052
Size: 2088 4252
NotI GC'GGCC_GC
Cuts at: 3787 3787
Size: 6340
NspI r_CATG'y
Cuts at: 1132 1229 2124 3698 5243 1132
Size: 97 895 1574 1545 2229
Fragments arranged by size:
2229 1574 1545 895 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6340
PciI A'CATG_T
Cuts at: 2120 5239 2120
Size: 3119 3221
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3236 1108
Size: 2128 4212
PfoI T'CCnGG_A
Cuts at: 2340 3177 3210 2340
Size: 837 33 5470
Fragments arranged by size:
5470 837 33
PmeI GTTT'AAAC
Cuts at: 3644 3644
Size: 6340
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3159 3191 4409 4441 5922 5954 3159
Size: 32 1218 32 1481 32 3545
Fragments arranged by size:
3545 1481 1218 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2129 2129
Size: 6340
PshAI GACnn'nnGTC
Cuts at: 1613 2134 1613
Size: 521 5819
PsiI TTA'TAA
Cuts at: 3522 3910 3522
Size: 388 5952
PspOMI G'GGCC_C
Cuts at: 2988 2988
Size: 6340
PstI C_TGCA'G
Cuts at: 830 3516 3692 830
Size: 2686 176 3478
Fragments arranged by size:
3478 2686 176
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6340
PvuII CAG'CTG
Cuts at: 3223 3223
Size: 6340
SalI G'TCGA_C
Cuts at: 1936 2435 3682 1936
Size: 499 1247 4594
Fragments arranged by size:
4594 1247 499
SanDI GG'GwC_CC
Cuts at: 2129 2129
Size: 6340
SbfI CC_TGCA'GG
Cuts at: 3692 3692
Size: 6340
ScaI AGT'ACT
Cuts at: 4588 6300 4588
Size: 1712 4628
SfcI C'TryA_G
Cuts at: 826 1045 2325 3512 3688 4823 5504 5695 826
Size: 219 1280 1187 176 1135 681 191 1471
Fragments arranged by size:
1471 1280 1187 1135 681 219 191 176
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6340
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6340
SmaI CCC'GGG
Cuts at: 1872 3667 1872
Size: 1795 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4444 5345 5607 820
Size: 197 926 2501 901 262 1553
Fragments arranged by size:
2501 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4131 493
Size: 3638 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6340
SphI G_CATG'C
Cuts at: 1132 3698 1132
Size: 2566 3774
SspI AAT'ATT
Cuts at: 5 52 4264 5
Size: 47 4212 2081
Fragments arranged by size:
4212 2081 47
StuI AGG'CCT
Cuts at: 1517 3048 3116 1517
Size: 1531 68 4741
Fragments arranged by size:
4741 1531 68
StyI C'CwwG_G
Cuts at: 513 1065 3111 3282 3779 513
Size: 552 2046 171 497 3074
Fragments arranged by size:
3074 2046 552 497 171
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2373 3431 4367 4384 4537 4722 5167
Size: 1255 793 1058 936 17 153 185 445
Cuts at: 5167 325
Size: 1498
Fragments arranged by size:
1498 1255 1058 936 793 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2823 4586 6298
Size: 274 80 33 51 1443 846 1763 1712
Cuts at: 6298 96
Size: 138
Fragments arranged by size:
1763 1712 1443 846 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2160 2192 6300 6332 2160
Size: 32 4108 32 2168
Fragments arranged by size:
4108 2168 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6340
XbaI T'CTAG_A
Cuts at: 3676 3676
Size: 6340
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3295 3295
Size: 6340
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6340
XmaI C'CCGG_G
Cuts at: 1870 3665 1870
Size: 1795 4545
XmnI GAAnn'nnTTC
Cuts at: 4469 4469
Size: 6340
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4148 276
Size: 53 83 186 936 2614 2468
Fragments arranged by size:
2614 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I
EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BbeI BfrBI BsiWI BstZ17I
Bsu36I DraIII EcoICRI EcoNI FalI FseI FspAI KasI
NarI NruI NsiI PacI PmlI PspXI PsrI RsrII
SacI SacII SapI SexAI SfiI SfoI SrfI SwaI