Restriction map for
pFN21AB3179
(Circular) (Six-base) MAPSORT of: pFN21AB3179.seq Check: 8544 from: 1 to: 9301
pFN21AB3179 9301 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 5194 5194
Size: 9301
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 7111 278
Size: 53 83 186 936 5575 2468
Fragments arranged by size:
5575 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2144 2579 2655 5332 6644 1937
Size: 207 435 76 2677 1312 4594
Fragments arranged by size:
4594 2677 1312 435 207 76
Acc65I G'GTAC_C
Cuts at: 5390 6622 5390
Size: 1232 8069
AclI AA'CG_TT
Cuts at: 1563 7428 7801 1563
Size: 5865 373 3063
Fragments arranged by size:
5865 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2056 2375 4041 4071 4971 5009 6024 7364
Size: 424 319 1666 30 900 38 1015 1340
Cuts at: 7364 8748 9016 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1666 1384 1340 1015 900 424 319
268 38 30
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 9104
AflIII A'CryG_T
Cuts at: 1203 2344 3618 8200 9177 1203
Size: 1141 1274 4582 977 1327
Fragments arranged by size:
4582 1327 1274 1141 977
AgeI A'CCGG_T
Cuts at: 8132 8132
Size: 9301
AhdI GACnn_n'nnGTC
Cuts at: 8030 8030
Size: 9301
AleI CACnn'nnGTG
Cuts at: 1166 6078 1166
Size: 4912 4389
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3214 4307 1184
Size: 2030 1093 6178
Fragments arranged by size:
6178 2030 1093
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3801 3833 3801
Size: 32 9269
AlwNI CAG_nnn'CTG
Cuts at: 7044 8616 7044
Size: 1572 7729
ApaI G_GGCC'C
Cuts at: 4488 6416 4488
Size: 1928 7373
ApaLI G'TGCA_C
Cuts at: 7358 8514 7358
Size: 1156 8145
ApoI r'AATT_y
Cuts at: 1493 2028 2186 2722 2848 2903 3148 3637 4384
Size: 535 158 536 126 55 245 489 747
Cuts at: 4384 4511 4587 4671 5159 5433 6282 6610 6836
Size: 127 76 84 488 274 849 328 226
Cuts at: 6836 6988 9097 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 849 747 536 535 489 488
328 274 245 226 158 152 127 126
84 76 55
AseI AT'TA_AT
Cuts at: 160 1032 5036 7855 160
Size: 872 4004 2819 1606
Fragments arranged by size:
4004 2819 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9301
AvaI C'yCGr_G
Cuts at: 1870 1943 6626 1870
Size: 73 4683 4545
Fragments arranged by size:
4683 4545 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 5437 5470 5437
Size: 33 9268
BamHI G'GATC_C
Cuts at: 2685 3809 6631 6998 2685
Size: 1124 2822 367 4988
Fragments arranged by size:
4988 2822 1124 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 5390 6622 8077 618
Size: 325 217 627 3603 1232 1455 1842
Fragments arranged by size:
3603 1842 1455 1232 627 325 217
BanII G_rGCy'C
Cuts at: 1391 4488 6177 6416 6620 1391
Size: 3097 1689 239 204 4072
Fragments arranged by size:
4072 3097 1689 239 204
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2953 4191 4802 6039 928
Size: 971 1054 1238 611 1237 4190
Fragments arranged by size:
4190 1238 1237 1054 971 611
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 7492 7526 1903
Size: 34 5555 34 3678
Fragments arranged by size:
5555 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5056 5302 5495 5562 7193 8403 5056
Size: 246 193 67 1631 1210 5954
Fragments arranged by size:
5954 1631 1210 246 193 67
BclI T'GATC_A
Cuts at: 1548 2836 1548
Size: 1288 8013
BfrBI ATG'CAT
Cuts at: 6296 6296
Size: 9301
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 7912 136
Size: 107 122 71 631 605 6240 1525
Fragments arranged by size:
6240 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3688 4886 9296 3688
Size: 1198 4410 3693
Fragments arranged by size:
4410 3693 1198
BlpI GC'TnA_GC
Cuts at: 4710 6718 4710
Size: 2008 7293
Bme1580I G_kGCm'C
Cuts at: 1790 3526 4488 5537 6416 7362 8518 1790
Size: 1736 962 1049 879 946 1156 2573
Fragments arranged by size:
2573 1736 1156 1049 962 946 879
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 9301
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3725 5432 5531
Size: 416 53 83 399 24 2308 1707 99
Cuts at: 5531 6523 7032 7990 442
Size: 992 509 958 1753
Fragments arranged by size:
2308 1753 1707 992 958 509 416 399
99 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9301
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2184 4488 4512 4678 4911 7961 1239
Size: 945 2304 24 166 233 3050 2579
Fragments arranged by size:
3050 2579 2304 945 233 166 24
Bpu10I CC'TnA_GC
Cuts at: 6134 6171 6134
Size: 37 9264
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5513 6500 6602 7426 8291 8589 5513
Size: 987 102 824 865 298 6225
Fragments arranged by size:
6225 987 865 824 298 102
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5411 5974 7964 882
Size: 617 3912 563 1990 2219
Fragments arranged by size:
3912 2219 1990 617 563
BsaAI yAC'GTr
Cuts at: 493 1204 2345 3607 7092 493
Size: 711 1141 1262 3485 2702
Fragments arranged by size:
3485 2702 1262 1141 711
BsaBI GATnn'nnATC
Cuts at: 4738 9295 4738
Size: 4557 4744
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 7108 7490 8125 275
Size: 53 83 186 936 5575 382 635 1451
Fragments arranged by size:
5575 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 7734 8132 8406 8553 8964 7734
Size: 398 274 147 411 8071
Fragments arranged by size:
8071 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3459 3489 3789 3819 1178
Size: 30 165 30 2056 30 300 30 6660
Fragments arranged by size:
6660 2056 300 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3810 1186
Size: 2624 6677
BseYI C'CCAG_C
Cuts at: 3462 5298 5827 8504 9000 3462
Size: 1836 529 2677 496 3763
Fragments arranged by size:
3763 2677 1836 529 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 8728
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6751 7512 8540 1540
Size: 275 186 4750 761 1028 2301
Fragments arranged by size:
4750 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2764 6177 7362 7447 8518 2764
Size: 3413 1185 85 1071 3547
Fragments arranged by size:
3547 3413 1185 1071 85
BsmI GAATG_Cn'
Cuts at: 3116 5671 6529 6812 6905 3116
Size: 2555 858 283 93 5512
Fragments arranged by size:
5512 2555 858 283 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2764 3526 4488 5537 6177 6416 6620
Size: 399 974 762 962 1049 640 239 204
Cuts at: 6620 7362 7447 8518 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 1049 974 962 762 742 640
399 239 204 85
BspEI T'CCGG_A
Cuts at: 8964 8964
Size: 9301
BspHI T'CATG_A
Cuts at: 3763 5929 7190 8920 3763
Size: 2166 1261 1730 4144
Fragments arranged by size:
4144 2166 1730 1261
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2749 2827 4796 5194 6656 844
Size: 1905 78 1969 398 1462 3489
Fragments arranged by size:
3489 1969 1905 1462 398 78
BsrBI CCG'CTC
Cuts at: 1394 1823 7188 1394
Size: 429 5365 3507
Fragments arranged by size:
5365 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2489 3057 3750 4055 5246 5631 6162
Size: 1183 1241 568 693 305 1191 385 531
Cuts at: 6162 7796 7970 65
Size: 1634 174 1396
Fragments arranged by size:
1634 1396 1241 1191 1183 693 568 531
385 305 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4920 7945 8132 1700
Size: 110 3110 3025 187 2869
Fragments arranged by size:
3110 3025 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 9301
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9301
BssSI C'ACGA_G
Cuts at: 7361 8373 7361
Size: 1012 8289
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3749 3959 794
Size: 2955 210 6136
Fragments arranged by size:
6136 2955 210
BstBI TT'CG_AA
Cuts at: 2026 3409 4594 8105 2026
Size: 1383 1185 3511 3222
Fragments arranged by size:
3511 3222 1383 1185
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 9301
BstXI CCAn_nnnn'nTGG
Cuts at: 5059 6157 6409 5059
Size: 1098 252 7951
Fragments arranged by size:
7951 1098 252
BstYI r'GATC_y
Cuts at: 1972 2685 3016 3688 3809 4657 4722 4886 4995
Size: 713 331 672 121 848 65 164 109
Cuts at: 4995 6256 6485 6631 6998 7383 7400 8852 8938
Size: 1261 229 146 367 385 17 1452 86
Cuts at: 8938 8950 9296 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1261 848 713 672 385 367
346 331 229 164 146 121 109 86
65 17 12
BstZ17I GTA'TAC
Cuts at: 2145 2580 5333 2145
Size: 435 2753 6113
Fragments arranged by size:
6113 2753 435
Bsu36I CC'TnA_GG
Cuts at: 3443 5396 3443
Size: 1953 7348
BtgI C'CryG_G
Cuts at: 513 1065 2004 3238 3324 513
Size: 552 939 1234 86 6490
Fragments arranged by size:
6490 1234 939 552 86
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 8562
Fragments arranged by size:
8562 683 56
BtsI GCAGTG_nn'
Cuts at: 5486 6817 7611 7631 5486
Size: 1331 794 20 7156
Fragments arranged by size:
7156 1331 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 9301
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 6144 6179 529
Size: 35 5580 35 3651
Fragments arranged by size:
5580 3651 35 35
DraI TTT'AAA
Cuts at: 4627 6048 6605 4627
Size: 1421 557 7323
Fragments arranged by size:
7323 1421 557
DraIII CAC_nnn'GTG
Cuts at: 4041 4041
Size: 9301
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 8308 809
Size: 507 6992 1802
Fragments arranged by size:
6992 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3235 6748 7637
Size: 54 1425 50 275 18 1405 3513 889
Cuts at: 7637 9266 8
Size: 1629 43
Fragments arranged by size:
3513 1629 1425 1405 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6748 1537
Size: 275 4936 4090
Fragments arranged by size:
4936 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2075 2394 3303 3411 3606 3762 3930 5061
Size: 722 319 909 108 195 156 168 1131
Cuts at: 5061 7231 1353
Size: 2170 3423
Fragments arranged by size:
3423 2170 1131 909 722 319 195 168
156 108
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 7888 8262 8408 387
Size: 1064 138 6299 374 146 1280
Fragments arranged by size:
6299 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 6175 6175
Size: 9301
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2056 2184 2375 4041 4071 4488 4512
Size: 393 424 128 191 1666 30 417 24
Cuts at: 4512 4678 4911 4971 5009 6024 7364 7961 8748
Size: 166 233 60 38 1015 1340 597 787
Cuts at: 8748 9016 1239
Size: 268 1524
Fragments arranged by size:
1666 1524 1340 1015 787 597 424 417
393 268 233 191 166 128 60 38
30 24
EcoNI CCTnn'n_nnAGG
Cuts at: 3829 6495 3829
Size: 2666 6635
EcoO109I rG'GnC_Cy
Cuts at: 2417 3254 4484 6347 2417
Size: 837 1230 1863 5371
Fragments arranged by size:
5371 1863 1230 837
EcoRI G'AATT_C
Cuts at: 2722 2848 4384 4587 6610 6988 2722
Size: 126 1536 203 2023 378 5035
Fragments arranged by size:
5035 2023 1536 378 203 126
EcoRV GAT'ATC
Cuts at: 4408 4408
Size: 9301
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2086 4349 4873 5328 2086
Size: 2263 524 455 6059
Fragments arranged by size:
6059 2263 524 455
FspI TGC'GCA
Cuts at: 7807 7807
Size: 9301
HaeII r_GCGC'y
Cuts at: 1678 8448 1678
Size: 6770 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 5389 5421 7942 7974 8016
Size: 32 135 32 3817 32 2521 32 42
Cuts at: 8016 8048 1373
Size: 32 2626
Fragments arranged by size:
3817 2626 2521 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2923 4648 6645 6891 669
Size: 988 281 985 1725 1997 246 3079
Fragments arranged by size:
3079 1997 1725 988 985 281 246
HindIII A'AGCT_T
Cuts at: 748 2888 3357 5086 6201 748
Size: 2140 469 1729 1115 3848
Fragments arranged by size:
3848 2140 1729 1115 469
HpaI GTT'AAC
Cuts at: 6891 6891
Size: 9301
KpnI G_GTAC'C
Cuts at: 5394 6626 5394
Size: 1232 8069
MfeI C'AATT_G
Cuts at: 3102 3939 5449 5767 6567 6900 3102
Size: 837 1510 318 800 333 5503
Fragments arranged by size:
5503 1510 837 800 333 318
MluI A'CGCG_T
Cuts at: 9177 9177
Size: 9301
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2237 2575 3603 3787 4564 8415 8599 1305
Size: 932 338 1028 184 777 3851 184 2007
Fragments arranged by size:
3851 2007 1028 932 777 338 184 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3237 10
Size: 54 1425 343 1405 6074
Fragments arranged by size:
6074 1425 1405 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2327 3885 5780 6078 6155 7259 7618
Size: 648 1161 1558 1895 298 77 1104 359
Cuts at: 7618 7777 518
Size: 159 2042
Fragments arranged by size:
2042 1895 1558 1161 1104 648 359 298
159 77
MspA1I CmG'CkG
Cuts at: 1606 5295 6546 6717 7394 8542 8787 1606
Size: 3689 1251 171 677 1148 245 2120
Fragments arranged by size:
3689 2120 1251 1148 677 245 171
NcoI C'CATG_G
Cuts at: 513 1065 2004 3238 3324 513
Size: 552 939 1234 86 6490
Fragments arranged by size:
6490 1234 939 552 86
NdeI CA'TA_TG
Cuts at: 387 2844 5974 387
Size: 2457 3130 3714
Fragments arranged by size:
3714 3130 2457
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9301
NotI GC'GGCC_GC
Cuts at: 6748 6748
Size: 9301
NsiI A_TGCA'T
Cuts at: 6298 6298
Size: 9301
NspI r_CATG'y
Cuts at: 1132 1229 3622 3860 3957 4571 5641 6659 8204
Size: 97 2393 238 97 614 1070 1018 1545
Cuts at: 8204 1132
Size: 2229
Fragments arranged by size:
2393 2229 1545 1070 1018 614 238 97
97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 9301
PciI A'CATG_T
Cuts at: 3618 8200 3618
Size: 4582 4719
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2162 5843 1108
Size: 1054 3681 4566
Fragments arranged by size:
4566 3681 1054
PfoI T'CCnGG_A
Cuts at: 2161 2688 4528 5487 2161
Size: 527 1840 959 5975
Fragments arranged by size:
5975 1840 959 527
PmeI GTTT'AAAC
Cuts at: 6605 6605
Size: 9301
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2084 2116 7370 7402 8883 8915 2084
Size: 32 5254 32 1481 32 2470
Fragments arranged by size:
5254 2470 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2417 3254 6347 2417
Size: 837 3093 5371
Fragments arranged by size:
5371 3093 837
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 9301
PsiI TTA'TAA
Cuts at: 6871 6871
Size: 9301
PspOMI G'GGCC_C
Cuts at: 4484 6412 4484
Size: 1928 7373
PstI C_TGCA'G
Cuts at: 830 5263 5481 5722 6653 830
Size: 4433 218 241 931 3478
Fragments arranged by size:
4433 3478 931 241 218
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9301
PvuII CAG'CTG
Cuts at: 6546 6546
Size: 9301
SacI G_AGCT'C
Cuts at: 6177 6177
Size: 9301
SalI G'TCGA_C
Cuts at: 1936 6643 1936
Size: 4707 4594
SapI GCTCTTCn'nnn_
Cuts at: 3930 3930
Size: 9301
SbfI CC_TGCA'GG
Cuts at: 6653 6653
Size: 9301
ScaI AGT'ACT
Cuts at: 3934 6533 7549 9261 3934
Size: 2599 1016 1712 3974
Fragments arranged by size:
3974 2599 1712 1016
SexAI A'CCwGG_T
Cuts at: 3284 3284
Size: 9301
SfcI C'TryA_G
Cuts at: 826 1045 2096 3045 5259 5477 5556 5718 6183
Size: 219 1051 949 2214 218 79 162 465
Cuts at: 6183 6435 6649 7784 8465 8656 826
Size: 252 214 1135 681 191 1471
Fragments arranged by size:
2214 1471 1135 1051 949 681 465 252
219 218 214 191 162 79
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9301
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9301
SmaI CCC'GGG
Cuts at: 1872 6628 1872
Size: 4756 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5528 6479 6581 7405 8306 8568
Size: 197 926 3585 951 102 824 901 262
Cuts at: 8568 820
Size: 1553
Fragments arranged by size:
3585 1553 951 926 901 824 262 197
102
SnaBI TAC'GTA
Cuts at: 493 7092 493
Size: 6599 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9301
SphI G_CATG'C
Cuts at: 1132 6659 1132
Size: 5527 3774
SspI AAT'ATT
Cuts at: 5 52 5371 5917 7225 5
Size: 47 5319 546 1308 2081
Fragments arranged by size:
5319 2081 1308 546 47
StuI AGG'CCT
Cuts at: 1517 3835 6139 1517
Size: 2318 2304 4679
Fragments arranged by size:
4679 2318 2304
StyI C'CwwG_G
Cuts at: 513 1065 2004 2373 3238 3324 4146 6116 6238
Size: 552 939 369 865 86 822 1970 122
Cuts at: 6238 6343 6491 6740 513
Size: 105 148 249 3074
Fragments arranged by size:
3074 1970 939 865 822 552 369 249
148 122 105 86
SwaI ATTT'AAAT
Cuts at: 6048 6048
Size: 9301
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5414 7328 7345 7498 7683 8128 325
Size: 1255 3834 1914 17 153 185 445 1498
Fragments arranged by size:
3834 1914 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3041 3615 3932
Size: 274 80 33 51 1443 1064 574 317
Cuts at: 3932 3971 6531 7547 9259 96
Size: 39 2560 1016 1712 138
Fragments arranged by size:
2560 1712 1443 1064 1016 574 317 274
138 80 51 39 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2589 2621 4203 4235 5450 5482 9261 9293 2589
Size: 32 1582 32 1215 32 3779 32 2597
Fragments arranged by size:
3779 2597 1582 1215 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3698 4910 1316
Size: 2382 1212 5707
Fragments arranged by size:
5707 2382 1212
XbaI T'CTAG_A
Cuts at: 4244 5518 6637 4244
Size: 1274 1119 6908
Fragments arranged by size:
6908 1274 1119
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4702 4702
Size: 9301
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 9301
XmaI C'CCGG_G
Cuts at: 1870 6626 1870
Size: 4756 4545
XmnI GAAnn'nnTTC
Cuts at: 4329 4431 4497 7430 4329
Size: 102 66 2933 6200
Fragments arranged by size:
6200 2933 102 66
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 7109 276
Size: 53 83 186 936 5575 2468
Fragments arranged by size:
5575 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BbeI BbvCI BplI BsiWI BsmBI
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PmlI PspXI PsrI RsrII SacII SanDI SfiI SfoI
SrfI