Restriction map for
pFN21AB3164
(Circular) (Six-base) MAPSORT of: pFN21AB3164.seq Check: 7535 from: 1 to: 7468
pFN21AB3164 7468 bp
With 182 enzymes: SgfI *
April 15, 2010 09:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2610 5278 278
Size: 53 83 186 936 1074 2668 2468
Fragments arranged by size:
2668 2468 1074 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2604 4811 1937
Size: 667 2207 4594
Fragments arranged by size:
4594 2207 667
Acc65I G'GTAC_C
Cuts at: 4789 4789
Size: 7468
AclI AA'CG_TT
Cuts at: 1563 5595 5968 1563
Size: 4032 373 3063
Fragments arranged by size:
4032 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2056 3000 3311 4019 4535 4589 5531 6915
Size: 424 944 311 708 516 54 942 1384
Cuts at: 6915 7183 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 944 942 708 516 424 311
268 54
AflII C'TTAA_G
Cuts at: 820 1017 2831 820
Size: 197 1814 5457
Fragments arranged by size:
5457 1814 197
AflIII A'CryG_T
Cuts at: 1203 2539 6367 7344 1203
Size: 1336 3828 977 1327
Fragments arranged by size:
3828 1336 1327 977
AgeI A'CCGG_T
Cuts at: 4284 6299 4284
Size: 2015 5453
AhdI GACnn_n'nnGTC
Cuts at: 6197 6197
Size: 7468
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7468
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2133 2792 1184
Size: 949 659 5860
Fragments arranged by size:
5860 949 659
AlwNI CAG_nnn'CTG
Cuts at: 2199 2380 3055 4480 4513 4612 5211 6783 2199
Size: 181 675 1425 33 99 599 1572 2884
Fragments arranged by size:
2884 1572 1425 675 599 181 99 33
ApaLI G'TGCA_C
Cuts at: 4729 5525 6681 4729
Size: 796 1156 5516
Fragments arranged by size:
5516 1156 796
ApoI r'AATT_y
Cuts at: 1493 2279 2894 2951 3249 3609 4777 5003 5155
Size: 786 615 57 298 360 1168 226 152
Cuts at: 5155 7264 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1168 786 615 360 298 226
152 57
AseI AT'TA_AT
Cuts at: 160 1032 6022 160
Size: 872 4990 1606
Fragments arranged by size:
4990 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7468
AvaI C'yCGr_G
Cuts at: 1870 1943 4793 1870
Size: 73 2850 4545
Fragments arranged by size:
4545 2850 73
BamHI G'GATC_C
Cuts at: 2782 2971 4798 5165 2782
Size: 189 1827 367 5085
Fragments arranged by size:
5085 1827 367 189
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2470 2563 4789 6244 618
Size: 325 217 627 683 93 2226 1455 1842
Fragments arranged by size:
2226 1842 1455 683 627 325 217 93
BanII G_rGCy'C
Cuts at: 1391 4725 4787 1391
Size: 3334 62 4072
Fragments arranged by size:
4072 3334 62
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3009 3177 3720 4076 4252 928
Size: 971 1110 168 543 356 176 4144
Fragments arranged by size:
4144 1110 971 543 356 176 168
BbvCI CC'TCA_GC
Cuts at: 2888 2888
Size: 7468
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4035 4069 5659 5693 1903
Size: 34 2098 34 1590 34 3678
Fragments arranged by size:
3678 2098 1590 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5360 6570 5360
Size: 1210 6258
BclI T'GATC_A
Cuts at: 1548 3961 1548
Size: 2413 5055
BfrBI ATG'CAT
Cuts at: 3562 3562
Size: 7468
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6079 136
Size: 107 122 71 631 605 4407 1525
Fragments arranged by size:
4407 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2176 2296 3157 3846 4159 7463 2176
Size: 120 861 689 313 3304 2181
Fragments arranged by size:
3304 2181 861 689 313 120
BlpI GC'TnA_GC
Cuts at: 4885 4885
Size: 7468
Bme1580I G_kGCm'C
Cuts at: 1790 4733 5529 6685 1790
Size: 2943 796 1156 2573
Fragments arranged by size:
2943 2573 1156 796
BmgBI CAC'GTC
Cuts at: 1318 2020 2540 4498 1318
Size: 702 520 1958 4288
Fragments arranged by size:
4288 1958 702 520
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2115 5199 6157
Size: 416 53 83 399 24 698 3084 958
Cuts at: 6157 442
Size: 1753
Fragments arranged by size:
3084 1753 958 698 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7468
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2553 3405 4469 4563 4727 6128 1239
Size: 1314 852 1064 94 164 1401 2579
Fragments arranged by size:
2579 1401 1314 1064 852 164 94
Bpu10I CC'TnA_GC
Cuts at: 2888 3671 2888
Size: 783 6685
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5593 6458 6756 5593
Size: 865 298 6305
Fragments arranged by size:
6305 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4019 4445 6131 882
Size: 617 2520 426 1686 2219
Fragments arranged by size:
2520 2219 1686 617 426
BsaAI yAC'GTr
Cuts at: 493 1204 5259 493
Size: 711 4055 2702
Fragments arranged by size:
4055 2702 711
BsaBI GATnn'nnATC
Cuts at: 3138 7462 3138
Size: 4324 3144
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2607 5275 5657 6292
Size: 53 83 186 936 1074 2668 382 635
Cuts at: 6292 275
Size: 1451
Fragments arranged by size:
2668 1451 1074 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4284 4458 5901 6299 6573 6720 7131 4284
Size: 174 1443 398 274 147 411 4621
Fragments arranged by size:
4621 1443 411 398 274 174 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2526 2556 2996 3026 3373
Size: 30 165 30 1123 30 440 30 347
Cuts at: 3373 3403 4303 4333 4561 4591 1178
Size: 30 900 30 228 30 4055
Fragments arranged by size:
4055 1123 900 440 347 228 165 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3004 3378 1186
Size: 1818 374 5276
Fragments arranged by size:
5276 1818 374
BseYI C'CCAG_C
Cuts at: 3772 6671 7167 3772
Size: 2899 496 4073
Fragments arranged by size:
4073 2899 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2943 4548 1163
Size: 573 1207 1605 4083
Fragments arranged by size:
4083 1605 1207 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4918 5679 6707 1540
Size: 275 186 2917 761 1028 2301
Fragments arranged by size:
2917 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3862 4725 4733 5529 5614 6685 3862
Size: 863 8 796 85 1071 4645
Fragments arranged by size:
4645 1071 863 796 85 8
BsmI GAATG_Cn'
Cuts at: 2265 3915 4196 4979 5072 2265
Size: 1650 281 783 93 4661
Fragments arranged by size:
4661 1650 783 281 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3862 4725 4733 4787 5529 5614 6685
Size: 399 2072 863 8 54 742 85 1071
Cuts at: 6685 1391
Size: 2174
Fragments arranged by size:
2174 2072 1071 863 742 399 85 54
8
BspEI T'CCGG_A
Cuts at: 4458 7131 4458
Size: 2673 4795
BspHI T'CATG_A
Cuts at: 4366 5357 7087 4366
Size: 991 1730 4747
Fragments arranged by size:
4747 1730 991
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4001 4823 844
Size: 3157 822 3489
Fragments arranged by size:
3489 3157 822
BsrBI CCG'CTC
Cuts at: 1394 1823 2839 5355 1394
Size: 429 1016 2516 3507
Fragments arranged by size:
3507 2516 1016 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4056 4557 5963 6137 65
Size: 1183 2808 501 1406 174 1396
Fragments arranged by size:
2808 1406 1396 1183 501 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4284 6112 6299 1700
Size: 110 2474 1828 187 2869
Fragments arranged by size:
2869 2474 1828 187 110
BsrGI T'GTAC_A
Cuts at: 96 2340 96
Size: 2244 5224
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7468
BssSI C'ACGA_G
Cuts at: 4421 4469 4724 5528 6540 4421
Size: 48 255 804 1012 5349
Fragments arranged by size:
5349 1012 804 255 48
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3667 4538 794
Size: 2873 871 3724
Fragments arranged by size:
3724 2873 871
BstBI TT'CG_AA
Cuts at: 2277 6272 2277
Size: 3995 3473
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7468
BstXI CCAn_nnnn'nTGG
Cuts at: 2727 2727
Size: 7468
BstYI r'GATC_y
Cuts at: 1972 2176 2296 2782 2971 3157 3846 4159 4464
Size: 204 120 486 189 186 689 313 305
Cuts at: 4464 4798 5165 5550 5567 7019 7105 7117 7463
Size: 334 367 385 17 1452 86 12 346
Cuts at: 7463 1972
Size: 1977
Fragments arranged by size:
1977 1452 689 486 385 367 346 334
313 305 204 189 186 120 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 3016 4506 3016
Size: 1490 5978
BtgI C'CryG_G
Cuts at: 513 1065 2004 2825 4062 513
Size: 552 939 821 1237 3919
Fragments arranged by size:
3919 1237 939 821 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3228 4445 487
Size: 683 56 2002 1217 3510
Fragments arranged by size:
3510 2002 1217 683 56
BtsI GCAGTG_nn'
Cuts at: 2732 3100 4984 5778 5798 2732
Size: 368 1884 794 20 4402
Fragments arranged by size:
4402 1884 794 368 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7468
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7433
DraI TTT'AAA
Cuts at: 3408 3519 4587 4772 3408
Size: 111 1068 185 6104
Fragments arranged by size:
6104 1068 185 111
DraIII CAC_nnn'GTG
Cuts at: 4235 4235
Size: 7468
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6475 809
Size: 507 5159 1802
Fragments arranged by size:
5159 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 4915 5804
Size: 54 1425 50 275 18 177 2908 889
Cuts at: 5804 7433 8
Size: 1629 43
Fragments arranged by size:
2908 1629 1425 889 275 177 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4915 1537
Size: 275 3103 4090
Fragments arranged by size:
4090 3103 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2976 3063 4608 5398 1353
Size: 1623 87 1545 790 3423
Fragments arranged by size:
3423 1623 1545 790 87
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6055 6429 6575 387
Size: 1064 138 4466 374 146 1280
Fragments arranged by size:
4466 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4723 4723
Size: 7468
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2056 2553 3000 3311 3405 4019 4469
Size: 393 424 497 447 311 94 614 450
Cuts at: 4469 4535 4563 4589 4727 5531 6128 6915 7183
Size: 66 28 26 138 804 597 787 268
Cuts at: 7183 1239
Size: 1524
Fragments arranged by size:
1524 804 787 614 597 497 450 447
424 393 311 268 138 94 66 28
26
EcoO109I rG'GnC_Cy
Cuts at: 2066 3103 4519 4747 2066
Size: 1037 1416 228 4787
Fragments arranged by size:
4787 1416 1037 228
EcoRI G'AATT_C
Cuts at: 4777 5155 4777
Size: 378 7090
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2086 2604 3608 2086
Size: 518 1004 5946
Fragments arranged by size:
5946 1004 518
FspI TGC'GCA
Cuts at: 5974 5974
Size: 7468
HaeII r_GCGC'y
Cuts at: 1678 6615 1678
Size: 4937 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2076 2108 2767 2799 2996
Size: 32 135 32 504 32 659 32 197
Cuts at: 2996 3028 3803 3831 3835 3863 4583 4615 6109
Size: 32 775 28 4 28 720 32 1494
Cuts at: 6109 6141 6183 6215 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1494 775 720 659 504 197 135
42 32 32 32 32 32 32 32
32 28 28 4
HincII GTy'rAC
Cuts at: 669 1657 1938 4812 5058 669
Size: 988 281 2874 246 3079
Fragments arranged by size:
3079 2874 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7468
HpaI GTT'AAC
Cuts at: 5058 5058
Size: 7468
KpnI G_GTAC'C
Cuts at: 4793 4793
Size: 7468
MfeI C'AATT_G
Cuts at: 5067 5067
Size: 7468
MluI A'CGCG_T
Cuts at: 7344 7344
Size: 7468
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2524 6582 6766 1305
Size: 1219 4058 184 2007
Fragments arranged by size:
4058 2007 1219 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5646
Fragments arranged by size:
5646 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2424 2774 3446 3600 3632 4061 4425
Size: 648 1258 350 672 154 32 429 364
Cuts at: 4425 5426 5785 5944 518
Size: 1001 359 159 2042
Fragments arranged by size:
2042 1258 1001 672 648 429 364 359
350 159 154 32
MspA1I CmG'CkG
Cuts at: 1606 2789 2887 2936 4095 4884 5561 6709 6954
Size: 1183 98 49 1159 789 677 1148 245
Cuts at: 6954 1606
Size: 2120
Fragments arranged by size:
2120 1183 1159 1148 789 677 245 98
49
NcoI C'CATG_G
Cuts at: 513 1065 2004 2825 4062 513
Size: 552 939 821 1237 3919
Fragments arranged by size:
3919 1237 939 821 552
NdeI CA'TA_TG
Cuts at: 387 2205 4171 4645 387
Size: 1818 1966 474 3210
Fragments arranged by size:
3210 1966 1818 474
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7468
NotI GC'GGCC_GC
Cuts at: 4915 4915
Size: 7468
NsiI A_TGCA'T
Cuts at: 3564 3564
Size: 7468
NspI r_CATG'y
Cuts at: 1132 1229 2773 4826 6371 1132
Size: 97 1544 2053 1545 2229
Fragments arranged by size:
2229 2053 1545 1544 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7468
PciI A'CATG_T
Cuts at: 6367 6367
Size: 7468
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3649 3779 1108
Size: 2541 130 4797
Fragments arranged by size:
4797 2541 130
PfoI T'CCnGG_A
Cuts at: 3129 3129
Size: 7468
PmeI GTTT'AAAC
Cuts at: 4772 4772
Size: 7468
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5537 5569 7050 7082 5537
Size: 32 1481 32 5923
Fragments arranged by size:
5923 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2066 3103 4519 4747 2066
Size: 1037 1416 228 4787
Fragments arranged by size:
4787 1416 1037 228
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7468
PsiI TTA'TAA
Cuts at: 5038 5038
Size: 7468
PstI C_TGCA'G
Cuts at: 830 3095 4012 4534 4617 4820 830
Size: 2265 917 522 83 203 3478
Fragments arranged by size:
3478 2265 917 522 203 83
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7468
PvuII CAG'CTG
Cuts at: 2789 2887 4095 2789
Size: 98 1208 6162
Fragments arranged by size:
6162 1208 98
SacI G_AGCT'C
Cuts at: 4725 4725
Size: 7468
SalI G'TCGA_C
Cuts at: 1936 4810 1936
Size: 2874 4594
SanDI GG'GwC_CC
Cuts at: 2066 4747 2066
Size: 2681 4787
SbfI CC_TGCA'GG
Cuts at: 4820 4820
Size: 7468
ScaI AGT'ACT
Cuts at: 2928 4403 5716 7428 2928
Size: 1475 1313 1712 2968
Fragments arranged by size:
2968 1712 1475 1313
SfcI C'TryA_G
Cuts at: 826 1045 2352 2601 3091 4008 4530 4613 4816
Size: 219 1307 249 490 917 522 83 203
Cuts at: 4816 5951 6632 6823 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1307 1135 917 681 522 490 249
219 203 191 83
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7468
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7468
SmaI CCC'GGG
Cuts at: 1872 4795 1872
Size: 2923 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2831 5572 6473 6735 820
Size: 197 926 888 2741 901 262 1553
Fragments arranged by size:
2741 1553 926 901 888 262 197
SnaBI TAC'GTA
Cuts at: 493 5259 493
Size: 4766 2702
SpeI A'CTAG_T
Cuts at: 152 4213 152
Size: 4061 3407
SphI G_CATG'C
Cuts at: 1132 2773 4826 1132
Size: 1641 2053 3774
Fragments arranged by size:
3774 2053 1641
SspI AAT'ATT
Cuts at: 5 52 4139 5392 5
Size: 47 4087 1253 2081
Fragments arranged by size:
4087 2081 1253 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7468
StyI C'CwwG_G
Cuts at: 513 1065 2004 2670 2720 2825 3882 4062 4907
Size: 552 939 666 50 105 1057 180 845
Cuts at: 4907 513
Size: 3074
Fragments arranged by size:
3074 1057 939 845 666 552 180 105
50
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2838 4293 5495 5512 5665 5850 6295
Size: 1255 1258 1455 1202 17 153 185 445
Cuts at: 6295 325
Size: 1498
Fragments arranged by size:
1498 1455 1258 1255 1202 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2168 2340 2926
Size: 274 80 33 51 1443 191 172 586
Cuts at: 2926 3220 4097 4125 4401 5714 7426 96
Size: 294 877 28 276 1313 1712 138
Fragments arranged by size:
1712 1443 1313 877 586 294 276 274
191 172 138 80 51 33 28
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2140 2172 3373 3405 4503 4535 4731 4763 7428
Size: 32 1201 32 1098 32 196 32 2665
Cuts at: 7428 7460 2140
Size: 32 2148
Fragments arranged by size:
2665 2148 1201 1098 196 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7468
XbaI T'CTAG_A
Cuts at: 4804 4804
Size: 7468
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3238 3469 3238
Size: 231 7237
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7468
XmaI C'CCGG_G
Cuts at: 1870 4793 1870
Size: 2923 4545
XmnI GAAnn'nnTTC
Cuts at: 3072 4602 5597 3072
Size: 1530 995 4943
Fragments arranged by size:
4943 1530 995
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2608 5276 276
Size: 53 83 186 936 1074 2668 2468
Fragments arranged by size:
2668 2468 1074 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NsiI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SacI SalI SanDI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BplI BsiWI BsmBI BstZ17I EcoNI EcoRV FseI FspAI
KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI
PspXI PsrI RsrII SacII SapI SexAI SfiI SfoI
SrfI SwaI