Restriction map for
pFN21AB3129
(Circular) (Six-base) MAPSORT of: pFN21AB3129.seq Check: 7835 from: 1 to: 5422
pFN21AB3129 5422 bp
With 182 enzymes: SgfI *
May 7, 2009 14:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2565 2565
Size: 5422
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3232 278
Size: 53 83 186 936 1696 2468
Fragments arranged by size:
2468 1696 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2765 1937
Size: 828 4594
Acc65I G'GTAC_C
Cuts at: 2743 2743
Size: 5422
AclI AA'CG_TT
Cuts at: 1563 3549 3922 1563
Size: 1986 373 3063
Fragments arranged by size:
3063 1986 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2401 2675 3485 4869 5137 1632
Size: 769 274 810 1384 268 1917
Fragments arranged by size:
1917 1384 810 769 274 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5225
AflIII A'CryG_T
Cuts at: 1203 4321 5298 1203
Size: 3118 977 1327
Fragments arranged by size:
3118 1327 977
AgeI A'CCGG_T
Cuts at: 4253 4253
Size: 5422
AhdI GACnn_n'nnGTC
Cuts at: 4151 4151
Size: 5422
AleI CACnn'nnGTG
Cuts at: 1166 2402 1166
Size: 1236 4186
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5422
AlwNI CAG_nnn'CTG
Cuts at: 3165 4737 3165
Size: 1572 3850
ApaLI G'TGCA_C
Cuts at: 3479 4635 3479
Size: 1156 4266
ApoI r'AATT_y
Cuts at: 1493 2731 2957 3109 5218 1493
Size: 1238 226 152 2109 1697
Fragments arranged by size:
2109 1697 1238 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3976 160
Size: 872 2944 1606
Fragments arranged by size:
2944 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5422
AvaI C'yCGr_G
Cuts at: 1870 1943 2122 2162 2190 2747 1870
Size: 73 179 40 28 557 4545
Fragments arranged by size:
4545 557 179 73 40 28
BamHI G'GATC_C
Cuts at: 2568 2752 3119 2568
Size: 184 367 4871
Fragments arranged by size:
4871 367 184
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2111 2240 2269 2553 2702
Size: 325 217 627 324 129 29 284 149
Cuts at: 2702 2743 4198 618
Size: 41 1455 1842
Fragments arranged by size:
1842 1455 627 325 324 284 217 149
129 41 29
BanII G_rGCy'C
Cuts at: 1391 2415 2741 1391
Size: 1024 326 4072
Fragments arranged by size:
4072 1024 326
BbeI G_GCGC'C
Cuts at: 2115 2244 2115
Size: 129 5293
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2663 928
Size: 971 764 3687
Fragments arranged by size:
3687 971 764
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2078 2112 3613 3647 1903
Size: 34 141 34 1501 34 3678
Fragments arranged by size:
3678 1501 141 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3314 4524 3314
Size: 1210 4212
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5422
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2304 4033 136
Size: 107 122 71 631 605 632 1729 1525
Fragments arranged by size:
1729 1525 632 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5417 5417
Size: 5422
BlpI GC'TnA_GC
Cuts at: 2261 2839 2261
Size: 578 4844
Bme1580I G_kGCm'C
Cuts at: 1790 2169 3483 4639 1790
Size: 379 1314 1156 2573
Fragments arranged by size:
2573 1314 1156 379
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5422
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2443 3153 4111
Size: 416 53 83 399 24 1026 710 958
Cuts at: 4111 442
Size: 1753
Fragments arranged by size:
1753 1026 958 710 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5422
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4082 1239
Size: 2843 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3547 4412 4710 3547
Size: 865 298 4259
Fragments arranged by size:
4259 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4085 882
Size: 617 2586 2219
Fragments arranged by size:
2586 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3213 493
Size: 711 2009 2702
Fragments arranged by size:
2702 2009 711
BsaBI GATnn'nnATC
Cuts at: 5416 5416
Size: 5422
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2112 2241 2369 3229
Size: 53 83 186 936 579 129 128 860
Cuts at: 3229 3611 4246 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 860 635 579 382 186 129
128 83 53
BsaWI w'CCGG_w
Cuts at: 3855 4253 4527 4674 5085 3855
Size: 398 274 147 411 4192
Fragments arranged by size:
4192 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2300 2330 1178
Size: 30 165 30 897 30 4270
Fragments arranged by size:
4270 897 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2143 2423 1186
Size: 957 280 4185
Fragments arranged by size:
4185 957 280
BseYI C'CCAG_C
Cuts at: 2015 2157 2586 2615 4625 5121 2015
Size: 142 429 29 2010 496 2316
Fragments arranged by size:
2316 2010 496 429 142 29
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2345 2543 1163
Size: 573 609 198 4042
Fragments arranged by size:
4042 609 573 198
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2202 2872 3633 4661 1540
Size: 275 186 201 670 761 1028 2301
Fragments arranged by size:
2301 1028 761 670 275 201 186
BsiHKAI G_wGCw'C
Cuts at: 2415 3483 3568 4639 2415
Size: 1068 85 1071 3198
Fragments arranged by size:
3198 1071 1068 85
BsmI GAATG_Cn'
Cuts at: 2933 3026 2933
Size: 93 5329
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2169 2415 2741 3483 3568 4639 1391
Size: 399 379 246 326 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 379 326 246 85
BspEI T'CCGG_A
Cuts at: 5085 5085
Size: 5422
BspHI T'CATG_A
Cuts at: 3311 5041 3311
Size: 1730 3692
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2565 2777 844
Size: 1721 212 3489
Fragments arranged by size:
3489 1721 212
BsrBI CCG'CTC
Cuts at: 1394 1823 3309 1394
Size: 429 1486 3507
Fragments arranged by size:
3507 1486 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3917 4091 65
Size: 1183 2669 174 1396
Fragments arranged by size:
2669 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2303 2550 4066 4253 1700
Size: 110 493 247 1516 187 2869
Fragments arranged by size:
2869 1516 493 247 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5422
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5422
BssSI C'ACGA_G
Cuts at: 3482 4494 3482
Size: 1012 4410
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5422
BstBI TT'CG_AA
Cuts at: 4226 4226
Size: 5422
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5422
BstXI CCAn_nnnn'nTGG
Cuts at: 2404 2404
Size: 5422
BstYI r'GATC_y
Cuts at: 1972 2568 2752 3119 3504 3521 4973 5059 5071
Size: 596 184 367 385 17 1452 86 12
Cuts at: 5071 5417 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 596 385 367 346 184 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2247 2397 513
Size: 552 939 243 150 3538
Fragments arranged by size:
3538 939 552 243 150
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2506 487
Size: 683 56 1280 3403
Fragments arranged by size:
3403 1280 683 56
BtsI GCAGTG_nn'
Cuts at: 2938 3732 3752 2938
Size: 794 20 4608
Fragments arranged by size:
4608 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5422
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5387
DraI TTT'AAA
Cuts at: 2726 2726
Size: 5422
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4429 809
Size: 507 3113 1802
Fragments arranged by size:
3113 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2869 3758 5387
Size: 54 1425 50 275 18 1039 889 1629
Cuts at: 5387 8
Size: 43
Fragments arranged by size:
1629 1425 1039 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2869 1537
Size: 275 1057 4090
Fragments arranged by size:
4090 1057 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2420 3352 1353
Size: 1067 932 3423
Fragments arranged by size:
3423 1067 932
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2285 4009 4383 4529 387
Size: 1064 138 696 1724 374 146 1280
Fragments arranged by size:
1724 1280 1064 696 374 146 138
EcoICRI GAG'CTC
Cuts at: 2413 2413
Size: 5422
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2401 2675 3485 4082 4869 5137 1239
Size: 393 769 274 810 597 787 268 1524
Fragments arranged by size:
1524 810 787 769 597 393 274 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2297 2297
Size: 5422
EcoO109I rG'GnC_Cy
Cuts at: 2194 2208 2252 2194
Size: 14 44 5364
Fragments arranged by size:
5364 44 14
EcoRI G'AATT_C
Cuts at: 2731 3109 2731
Size: 378 5044
FspI TGC'GCA
Cuts at: 3928 3928
Size: 5422
HaeII r_GCGC'y
Cuts at: 1678 2115 2244 4569 1678
Size: 437 129 2325 2531
Fragments arranged by size:
2531 2325 437 129
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2300 2332 2676 2708 4063
Size: 32 135 32 728 32 344 32 1355
Cuts at: 4063 4095 4137 4169 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1355 728 344 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2491 2766 3012 669
Size: 988 281 553 275 246 3079
Fragments arranged by size:
3079 988 553 281 275 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5422
HpaI GTT'AAC
Cuts at: 3012 3012
Size: 5422
KasI G'GCGC_C
Cuts at: 2111 2240 2111
Size: 129 5293
KpnI G_GTAC'C
Cuts at: 2747 2747
Size: 5422
MfeI C'AATT_G
Cuts at: 3021 3021
Size: 5422
MluI A'CGCG_T
Cuts at: 5298 5298
Size: 5422
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4536 4720 1305
Size: 3231 184 2007
Fragments arranged by size:
3231 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3600
Fragments arranged by size:
3600 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2402 2477 3380 3739 3898 518
Size: 648 1236 75 903 359 159 2042
Fragments arranged by size:
2042 1236 903 648 359 159 75
MspA1I CmG'CkG
Cuts at: 1606 2218 2249 2359 2838 3515 4663 4908 1606
Size: 612 31 110 479 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 612 479 245 110 31
NaeI GCC'GGC
Cuts at: 2305 2305
Size: 5422
NarI GG'CG_CC
Cuts at: 2112 2241 2112
Size: 129 5293
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3931
Fragments arranged by size:
3931 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5422
NgoMIV G'CCGG_C
Cuts at: 2303 2303
Size: 5422
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5422
NotI GC'GGCC_GC
Cuts at: 2869 2869
Size: 5422
NspI r_CATG'y
Cuts at: 1132 1229 2623 2780 4325 1132
Size: 97 1394 157 1545 2229
Fragments arranged by size:
2229 1545 1394 157 97
PasI CC'CwG_GG
Cuts at: 1348 2698 1348
Size: 1350 4072
PciI A'CATG_T
Cuts at: 4321 4321
Size: 5422
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5422
PfoI T'CCnGG_A
Cuts at: 2571 2571
Size: 5422
PmeI GTTT'AAAC
Cuts at: 2726 2726
Size: 5422
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3491 3523 5004 5036 3491
Size: 32 1481 32 3877
Fragments arranged by size:
3877 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2194 2208 2194
Size: 14 5408
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5422
PsiI TTA'TAA
Cuts at: 2067 2992 2067
Size: 925 4497
PstI C_TGCA'G
Cuts at: 830 2223 2774 830
Size: 1393 551 3478
Fragments arranged by size:
3478 1393 551
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5422
PvuII CAG'CTG
Cuts at: 2359 2359
Size: 5422
SacI G_AGCT'C
Cuts at: 2415 2415
Size: 5422
SacII CC_GC'GG
Cuts at: 2250 2250
Size: 5422
SalI G'TCGA_C
Cuts at: 1936 2764 1936
Size: 828 4594
SanDI GG'GwC_CC
Cuts at: 2194 2194
Size: 5422
SapI GCTCTTCn'nnn_
Cuts at: 2420 2420
Size: 5422
SbfI CC_TGCA'GG
Cuts at: 2774 2774
Size: 5422
ScaI AGT'ACT
Cuts at: 3670 5382 3670
Size: 1712 3710
SfcI C'TryA_G
Cuts at: 826 1045 2219 2770 3905 4586 4777 826
Size: 219 1174 551 1135 681 191 1471
Fragments arranged by size:
1471 1174 1135 681 551 219 191
SfoI GGC'GCC
Cuts at: 2113 2242 2113
Size: 129 5293
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5422
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5422
SmaI CCC'GGG
Cuts at: 1872 2124 2164 2192 2749 1872
Size: 252 40 28 557 4545
Fragments arranged by size:
4545 557 252 40 28
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3526 4427 4689 820
Size: 197 926 1583 901 262 1553
Fragments arranged by size:
1583 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3213 493
Size: 2720 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5422
SphI G_CATG'C
Cuts at: 1132 2623 2780 1132
Size: 1491 157 3774
Fragments arranged by size:
3774 1491 157
SrfI GCCC'GGGC
Cuts at: 2164 2164
Size: 5422
SspI AAT'ATT
Cuts at: 5 52 3346 5
Size: 47 3294 2081
Fragments arranged by size:
3294 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5422
StyI C'CwwG_G
Cuts at: 513 1065 2004 2861 513
Size: 552 939 857 3074
Fragments arranged by size:
3074 939 857 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2690 3449 3466 3619 3804 4249 325
Size: 1255 1110 759 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1110 759 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2055 3668 5380
Size: 274 80 33 51 1443 78 1613 1712
Cuts at: 5380 96
Size: 138
Fragments arranged by size:
1712 1613 1443 274 138 80 78 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2161 2193 2265 2297 5382 5414 2161
Size: 32 72 32 3085 32 2169
Fragments arranged by size:
3085 2169 72 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2198 1316
Size: 882 4540
XbaI T'CTAG_A
Cuts at: 2758 2758
Size: 5422
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5422
XmaI C'CCGG_G
Cuts at: 1870 2122 2162 2190 2747 1870
Size: 252 40 28 557 4545
Fragments arranged by size:
4545 557 252 40 28
XmnI GAAnn'nnTTC
Cuts at: 3551 3551
Size: 5422
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3230 276
Size: 53 83 186 936 1696 2468
Fragments arranged by size:
2468 1696 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SacI SacII SalI SanDI SapI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV
FalI FseI FspAI NruI NsiI PacI PmlI PspOMI
PspXI PsrI RsrII SexAI SfiI SwaI XcmI