Restriction map for
pFN21AB3126
(Circular) (Six-base) MAPSORT of: pFN21AB3126.seq Check: 1798 from: 1 to: 7609
pFN21AB3126 7609 bp
With 182 enzymes: SgfI *
February 27, 2009 14:45 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2334 2334
Size: 7609
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4035 5419 278
Size: 53 83 186 936 2499 1384 2468
Fragments arranged by size:
2499 2468 1384 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2425 4952 1937
Size: 488 2527 4594
Fragments arranged by size:
4594 2527 488
Acc65I G'GTAC_C
Cuts at: 4930 4930
Size: 7609
AclI AA'CG_TT
Cuts at: 1563 5736 6109 1563
Size: 4173 373 3063
Fragments arranged by size:
4173 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3354 4461 5672 7056 7324 1632
Size: 1722 1107 1211 1384 268 1917
Fragments arranged by size:
1917 1722 1384 1211 1107 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7412
AflIII A'CryG_T
Cuts at: 1203 6508 7485 1203
Size: 5305 977 1327
Fragments arranged by size:
5305 1327 977
AgeI A'CCGG_T
Cuts at: 6440 6440
Size: 7609
AhdI GACnn_n'nnGTC
Cuts at: 6338 6338
Size: 7609
AleI CACnn'nnGTG
Cuts at: 1166 3614 1166
Size: 2448 5161
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3561 3885 4010 4085 1184
Size: 2377 324 125 75 4708
Fragments arranged by size:
4708 2377 324 125 75
AlwNI CAG_nnn'CTG
Cuts at: 2987 4776 5352 6924 2987
Size: 1789 576 1572 3672
Fragments arranged by size:
3672 1789 1572 576
ApaLI G'TGCA_C
Cuts at: 2516 5666 6822 2516
Size: 3150 1156 3303
Fragments arranged by size:
3303 3150 1156
ApoI r'AATT_y
Cuts at: 1493 2805 4476 4918 5144 5296 7405 1493
Size: 1312 1671 442 226 152 2109 1697
Fragments arranged by size:
2109 1697 1671 1312 442 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2967 6163 160
Size: 872 1935 3196 1606
Fragments arranged by size:
3196 1935 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7609
AvaI C'yCGr_G
Cuts at: 1870 1943 4934 1870
Size: 73 2991 4545
Fragments arranged by size:
4545 2991 73
BamHI G'GATC_C
Cuts at: 2056 4939 5306 2056
Size: 2883 367 4359
Fragments arranged by size:
4359 2883 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2216 2691 3140 4838 4930
Size: 325 217 627 429 475 449 1698 92
Cuts at: 4930 6385 618
Size: 1455 1842
Fragments arranged by size:
1842 1698 1455 627 475 449 429 325
217 92
BanII G_rGCy'C
Cuts at: 1391 3417 4758 4928 1391
Size: 2026 1341 170 4072
Fragments arranged by size:
4072 2026 1341 170
BbeI G_GCGC'C
Cuts at: 2695 2695
Size: 7609
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2932 4697 928
Size: 971 1033 1765 3840
Fragments arranged by size:
3840 1765 1033 971
BbvCI CC'TCA_GC
Cuts at: 2630 3809 2630
Size: 1179 6430
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5800 5834 1903
Size: 34 3863 34 3678
Fragments arranged by size:
3863 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4524 5501 6711 4524
Size: 977 1210 5422
Fragments arranged by size:
5422 1210 977
BclI T'GATC_A
Cuts at: 1548 3774 1548
Size: 2226 5383
BfrBI ATG'CAT
Cuts at: 2744 2744
Size: 7609
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4025 6220 136
Size: 107 122 71 631 605 2353 2195 1525
Fragments arranged by size:
2353 2195 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7604 7604
Size: 7609
BlpI GC'TnA_GC
Cuts at: 5026 5026
Size: 7609
Bme1580I G_kGCm'C
Cuts at: 1790 2520 3194 5670 6826 1790
Size: 730 674 2476 1156 2573
Fragments arranged by size:
2573 2476 1156 730 674
BmgBI CAC'GTC
Cuts at: 1318 3577 1318
Size: 2259 5350
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4507 4679 5340
Size: 416 53 83 399 24 3090 172 661
Cuts at: 5340 6298 442
Size: 958 1753
Fragments arranged by size:
3090 1753 958 661 416 399 172 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7609
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4578 4578
Size: 7609
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2814 3171 3507 4142 4663 6269 1239
Size: 1575 357 336 635 521 1606 2579
Fragments arranged by size:
2579 1606 1575 635 521 357 336
Bpu10I CC'TnA_GC
Cuts at: 2630 3809 3827 4041 2630
Size: 1179 18 214 6198
Fragments arranged by size:
6198 1179 214 18
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3893 5734 6599 6897 3893
Size: 1841 865 298 4605
Fragments arranged by size:
4605 1841 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4600 6272 882
Size: 617 3101 1672 2219
Fragments arranged by size:
3101 2219 1672 617
BsaAI yAC'GTr
Cuts at: 493 1204 5400 493
Size: 711 4196 2702
Fragments arranged by size:
4196 2702 711
BsaBI GATnn'nnATC
Cuts at: 7603 7603
Size: 7609
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2692 2715 3755 4032
Size: 53 83 186 936 1159 23 1040 277
Cuts at: 4032 5416 5798 6433 275
Size: 1384 382 635 1451
Fragments arranged by size:
1451 1384 1159 1040 936 635 382 277
186 83 53 23
BsaWI w'CCGG_w
Cuts at: 2665 6042 6440 6714 6861 7272 2665
Size: 3377 398 274 147 411 3002
Fragments arranged by size:
3377 3002 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4037 4067 1178
Size: 30 165 30 2634 30 4720
Fragments arranged by size:
4720 2634 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3198 1186
Size: 2012 5597
BseYI C'CCAG_C
Cuts at: 2566 3287 3416 3815 4022 4184 6812 7308 2566
Size: 721 129 399 207 162 2628 496 2867
Fragments arranged by size:
2867 2628 721 496 399 207 162 129
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2356 3101 3638 4055 1163
Size: 573 620 745 537 417 4717
Fragments arranged by size:
4717 745 620 573 537 417
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5059 5820 6848 1540
Size: 275 186 3058 761 1028 2301
Fragments arranged by size:
3058 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2520 4089 4758 5670 5755 6826 2520
Size: 1569 669 912 85 1071 3303
Fragments arranged by size:
3303 1569 1071 912 669 85
BsmI GAATG_Cn'
Cuts at: 3967 5120 5213 3967
Size: 1153 93 6363
Fragments arranged by size:
6363 1153 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3567 3567
Size: 7609
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2520 3194 3417 4089 4758 4928 5670
Size: 399 730 674 223 672 669 170 742
Cuts at: 5670 5755 6826 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 730 674 672 669 399
223 170 85
BspEI T'CCGG_A
Cuts at: 2665 7272 2665
Size: 4607 3002
BspHI T'CATG_A
Cuts at: 5498 7228 5498
Size: 1730 5879
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2334 4342 4964 844
Size: 1490 2008 622 3489
Fragments arranged by size:
3489 2008 1490 622
BsrBI CCG'CTC
Cuts at: 1394 1823 5496 1394
Size: 429 3673 3507
Fragments arranged by size:
3673 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 6104 6278 65
Size: 1183 4856 174 1396
Fragments arranged by size:
4856 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2559 6253 6440 1700
Size: 110 749 3694 187 2869
Fragments arranged by size:
3694 2869 749 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7609
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7609
BssSI C'ACGA_G
Cuts at: 5669 6681 5669
Size: 1012 6597
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7609
BstBI TT'CG_AA
Cuts at: 6413 6413
Size: 7609
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7609
BstXI CCAn_nnnn'nTGG
Cuts at: 2398 2623 4204 4877 2398
Size: 225 1581 673 5130
Fragments arranged by size:
5130 1581 673 225
BstYI r'GATC_y
Cuts at: 1972 2056 2367 3685 4234 4939 5306 5691 5708
Size: 84 311 1318 549 705 367 385 17
Cuts at: 5708 7160 7246 7258 7604 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1318 705 549 385 367 346
311 86 84 17 12
BstZ17I GTA'TAC
Cuts at: 2426 2426
Size: 7609
Bsu36I CC'TnA_GG
Cuts at: 2250 2250
Size: 7609
BtgI C'CryG_G
Cuts at: 513 1065 2004 2200 2703 3306 513
Size: 552 939 196 503 603 4816
Fragments arranged by size:
4816 939 603 552 503 196
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6870
Fragments arranged by size:
6870 683 56
BtsI GCAGTG_nn'
Cuts at: 2111 3609 3777 4067 4844 5125 5919 5939 2111
Size: 1498 168 290 777 281 794 20 3781
Fragments arranged by size:
3781 1498 794 777 290 281 168 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7609
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7574
DraI TTT'AAA
Cuts at: 4913 4913
Size: 7609
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2402 6616 809
Size: 507 1086 4214 1802
Fragments arranged by size:
4214 1802 1086 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3591 4017 4338
Size: 54 1425 50 275 18 1761 426 321
Cuts at: 4338 5056 5945 7574 8
Size: 718 889 1629 43
Fragments arranged by size:
1761 1629 1425 889 718 426 321 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5056 1537
Size: 275 3244 4090
Fragments arranged by size:
4090 3244 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2856 3471 4218 5539 1353
Size: 1503 615 747 1321 3423
Fragments arranged by size:
3423 1503 1321 747 615
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2582 3171 6196 6570 6716 387
Size: 1064 138 993 589 3025 374 146 1280
Fragments arranged by size:
3025 1280 1064 993 589 374 146 138
EcoICRI GAG'CTC
Cuts at: 4756 4756
Size: 7609
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2814 3171 3354 3507 4142 4461 4663
Size: 393 1182 357 183 153 635 319 202
Cuts at: 4663 5672 6269 7056 7324 1239
Size: 1009 597 787 268 1524
Fragments arranged by size:
1524 1182 1009 787 635 597 393 357
319 268 202 183 153
EcoNI CCTnn'n_nnAGG
Cuts at: 4735 4896 4735
Size: 161 7448
EcoO109I rG'GnC_Cy
Cuts at: 3328 4627 4889 3328
Size: 1299 262 6048
Fragments arranged by size:
6048 1299 262
EcoRI G'AATT_C
Cuts at: 4918 5296 4918
Size: 378 7231
FspI TGC'GCA
Cuts at: 6115 6115
Size: 7609
HaeII r_GCGC'y
Cuts at: 1678 2695 3604 6756 1678
Size: 1017 909 3152 2531
Fragments arranged by size:
3152 2531 1017 909
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3076 3108 3302 3334 3727
Size: 32 135 32 1504 32 194 32 393
Cuts at: 3727 3759 4037 4069 4082 4114 4382 4414 4684
Size: 32 278 32 13 32 268 32 270
Cuts at: 4684 4716 6250 6282 6324 6356 1373
Size: 32 1534 32 42 32 2626
Fragments arranged by size:
2626 1534 1504 393 278 270 268 194
135 42 32 32 32 32 32 32
32 32 32 32 32 13
HincII GTy'rAC
Cuts at: 669 1657 1938 4953 5199 669
Size: 988 281 3015 246 3079
Fragments arranged by size:
3079 3015 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7609
HpaI GTT'AAC
Cuts at: 5199 5199
Size: 7609
KasI G'GCGC_C
Cuts at: 2691 2691
Size: 7609
KpnI G_GTAC'C
Cuts at: 4934 4934
Size: 7609
MfeI C'AATT_G
Cuts at: 4656 5208 4656
Size: 552 7057
MluI A'CGCG_T
Cuts at: 7485 7485
Size: 7609
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2772 6723 6907 1305
Size: 1467 3951 184 2007
Fragments arranged by size:
3951 2007 1467 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4340 10
Size: 54 1425 343 2508 3279
Fragments arranged by size:
3279 2508 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2328 3543 3614 4875 5567 5926 6085
Size: 648 1162 1215 71 1261 692 359 159
Cuts at: 6085 518
Size: 2042
Fragments arranged by size:
2042 1261 1215 1162 692 648 359 159
71
MspA1I CmG'CkG
Cuts at: 1606 2566 2780 4022 4184 4347 4435 4459 4785
Size: 960 214 1242 162 163 88 24 326
Cuts at: 4785 5025 5702 6850 7095 1606
Size: 240 677 1148 245 2120
Fragments arranged by size:
2120 1242 1148 960 677 326 245 240
214 163 162 88 24
NarI GG'CG_CC
Cuts at: 2692 2692
Size: 7609
NcoI C'CATG_G
Cuts at: 513 1065 2004 2200 3306 513
Size: 552 939 196 1106 4816
Fragments arranged by size:
4816 1106 939 552 196
NdeI CA'TA_TG
Cuts at: 387 4381 387
Size: 3994 3615
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7609
NotI GC'GGCC_GC
Cuts at: 5056 5056
Size: 7609
NsiI A_TGCA'T
Cuts at: 2746 2746
Size: 7609
NspI r_CATG'y
Cuts at: 1132 1229 2333 3166 3542 4967 6512 1132
Size: 97 1104 833 376 1425 1545 2229
Fragments arranged by size:
2229 1545 1425 1104 833 376 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7609
PciI A'CATG_T
Cuts at: 6508 6508
Size: 7609
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2123 2201 2341 3230 4347 1108
Size: 1015 78 140 889 1117 4370
Fragments arranged by size:
4370 1117 1015 889 140 78
PfoI T'CCnGG_A
Cuts at: 2503 3265 4119 2503
Size: 762 854 5993
Fragments arranged by size:
5993 854 762
PmeI GTTT'AAAC
Cuts at: 4913 4913
Size: 7609
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4312 4344 5678 5710 7191 7223 4312
Size: 32 1334 32 1481 32 4698
Fragments arranged by size:
4698 1481 1334 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3328 4889 3328
Size: 1561 6048
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7609
PsiI TTA'TAA
Cuts at: 5179 5179
Size: 7609
PstI C_TGCA'G
Cuts at: 830 2610 4458 4961 830
Size: 1780 1848 503 3478
Fragments arranged by size:
3478 1848 1780 503
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7609
PvuII CAG'CTG
Cuts at: 2566 2780 4184 4347 4435 4459 2566
Size: 214 1404 163 88 24 5716
Fragments arranged by size:
5716 1404 214 163 88 24
SacI G_AGCT'C
Cuts at: 4758 4758
Size: 7609
SalI G'TCGA_C
Cuts at: 1936 4951 1936
Size: 3015 4594
SapI GCTCTTCn'nnn_
Cuts at: 4218 4218
Size: 7609
SbfI CC_TGCA'GG
Cuts at: 4961 4961
Size: 7609
ScaI AGT'ACT
Cuts at: 4147 5857 7569 4147
Size: 1710 1712 4187
Fragments arranged by size:
4187 1712 1710
SexAI A'CCwGG_T
Cuts at: 2212 2212
Size: 7609
SfcI C'TryA_G
Cuts at: 826 1045 2606 4454 4957 6092 6773 6964 826
Size: 219 1561 1848 503 1135 681 191 1471
Fragments arranged by size:
1848 1561 1471 1135 681 503 219 191
SfoI GGC'GCC
Cuts at: 2693 2693
Size: 7609
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7609
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7609
SmaI CCC'GGG
Cuts at: 1872 4936 1872
Size: 3064 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3908 5713 6614 6876 820
Size: 197 926 1965 1805 901 262 1553
Fragments arranged by size:
1965 1805 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5400 493
Size: 4907 2702
SpeI A'CTAG_T
Cuts at: 152 3026 152
Size: 2874 4735
SphI G_CATG'C
Cuts at: 1132 2333 3542 4967 1132
Size: 1201 1209 1425 3774
Fragments arranged by size:
3774 1425 1209 1201
SspI AAT'ATT
Cuts at: 5 52 2079 2142 2971 5533 5
Size: 47 2027 63 829 2562 2081
Fragments arranged by size:
2562 2081 2027 829 63 47
StuI AGG'CCT
Cuts at: 1517 2035 1517
Size: 518 7091
StyI C'CwwG_G
Cuts at: 513 1065 2004 2200 2391 2616 3306 5048 513
Size: 552 939 196 191 225 690 1742 3074
Fragments arranged by size:
3074 1742 939 690 552 225 196 191
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5636 5653 5806 5991 6436 325
Size: 1255 4056 17 153 185 445 1498
Fragments arranged by size:
4056 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3200 4145 5855
Size: 274 80 33 51 1443 1223 945 1710
Cuts at: 5855 7567 96
Size: 1712 138
Fragments arranged by size:
1712 1710 1443 1223 945 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2499 2531 7569 7601 2499
Size: 32 5038 32 2507
Fragments arranged by size:
5038 2507 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3638 4772 1316
Size: 2322 1134 4153
Fragments arranged by size:
4153 2322 1134
XbaI T'CTAG_A
Cuts at: 3508 4330 4894 4945 3508
Size: 822 564 51 6172
Fragments arranged by size:
6172 822 564 51
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2398 4494 4576 2398
Size: 2096 82 5431
Fragments arranged by size:
5431 2096 82
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7609
XmaI C'CCGG_G
Cuts at: 1870 4934 1870
Size: 3064 4545
XmnI GAAnn'nnTTC
Cuts at: 2094 5738 2094
Size: 3644 3965
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4033 5417 276
Size: 53 83 186 936 2499 1384 2468
Fragments arranged by size:
2499 2468 1384 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BsiWI DraIII
EcoRV FalI FseI FspAI NaeI NgoMIV NruI PacI
PmlI PspOMI PspXI PsrI RsrII SacII SanDI SfiI
SrfI SwaI