Restriction map for
pFN21AB3114
(Circular) (Six-base) MAPSORT of: pFN21AB3114.seq Check: 9115 from: 1 to: 6340
pFN21AB3114 6340 bp
With 182 enzymes: SgfI *
February 27, 2009 14:34 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4150 278
Size: 53 83 186 936 2614 2468
Fragments arranged by size:
2614 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2154 3683 1937
Size: 217 1529 4594
Fragments arranged by size:
4594 1529 217
Acc65I G'GTAC_C
Cuts at: 3661 3661
Size: 6340
AclI AA'CG_TT
Cuts at: 1563 4467 4840 1563
Size: 2904 373 3063
Fragments arranged by size:
3063 2904 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2256 2298 2552 3072 3515 3633 4403 5787
Size: 624 42 254 520 443 118 770 1384
Cuts at: 5787 6055 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 770 624 520 443 268 254
118 42
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6143
AflIII A'CryG_T
Cuts at: 1203 2997 5239 6216 1203
Size: 1794 2242 977 1327
Fragments arranged by size:
2242 1794 1327 977
AgeI A'CCGG_T
Cuts at: 5171 5171
Size: 6340
AhdI GACnn_n'nnGTC
Cuts at: 5069 5069
Size: 6340
AleI CACnn'nnGTG
Cuts at: 1166 2883 3392 1166
Size: 1717 509 4114
Fragments arranged by size:
4114 1717 509
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6340
AlwNI CAG_nnn'CTG
Cuts at: 2095 2450 4083 5655 2095
Size: 355 1633 1572 2780
Fragments arranged by size:
2780 1633 1572 355
ApaLI G'TGCA_C
Cuts at: 4397 5553 4397
Size: 1156 5184
ApoI r'AATT_y
Cuts at: 1493 2354 2388 2748 2932 3649 3875 4027 6136
Size: 861 34 360 184 717 226 152 2109
Cuts at: 6136 1493
Size: 1697
Fragments arranged by size:
2109 1697 861 717 360 226 184 152
34
AseI AT'TA_AT
Cuts at: 160 1032 4894 160
Size: 872 3862 1606
Fragments arranged by size:
3862 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6340
AvaI C'yCGr_G
Cuts at: 1870 1943 3665 1870
Size: 73 1722 4545
Fragments arranged by size:
4545 1722 73
BamHI G'GATC_C
Cuts at: 3670 4037 3670
Size: 367 5973
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3661 5116 618
Size: 325 217 627 1874 1455 1842
Fragments arranged by size:
1874 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2247 3659 1391
Size: 856 1412 4072
Fragments arranged by size:
4072 1412 856
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2227 3603 928
Size: 971 328 1376 3665
Fragments arranged by size:
3665 1376 971 328
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2480 2514 4531 4565 1903
Size: 34 543 34 2017 34 3678
Fragments arranged by size:
3678 2017 543 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2224 4232 5442 2224
Size: 2008 1210 3122
Fragments arranged by size:
3122 2008 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6340
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4951 136
Size: 107 122 71 631 605 3279 1525
Fragments arranged by size:
3279 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6335 6335
Size: 6340
BlpI GC'TnA_GC
Cuts at: 3757 3757
Size: 6340
Bme1580I G_kGCm'C
Cuts at: 1790 4401 5557 1790
Size: 2611 1156 2573
Fragments arranged by size:
2611 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6340
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3241 4071 5029
Size: 416 53 83 399 24 1824 830 958
Cuts at: 5029 442
Size: 1753
Fragments arranged by size:
1824 1753 958 830 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6340
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3361 3361
Size: 6340
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2971 5000 1239
Size: 1732 2029 2579
Fragments arranged by size:
2579 2029 1732
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2888 4465 5330 5628 2888
Size: 1577 865 298 3600
Fragments arranged by size:
3600 1577 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5003 882
Size: 617 3504 2219
Fragments arranged by size:
3504 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2881 4131 493
Size: 711 1677 1250 2702
Fragments arranged by size:
2702 1677 1250 711
BsaBI GATnn'nnATC
Cuts at: 6334 6334
Size: 6340
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4147 4529 5164 275
Size: 53 83 186 936 2614 382 635 1451
Fragments arranged by size:
2614 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4773 5171 5445 5592 6003 4773
Size: 398 274 147 411 5110
Fragments arranged by size:
5110 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6115
Fragments arranged by size:
6115 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3176 1186
Size: 1990 4350
BseYI C'CCAG_C
Cuts at: 3112 5543 6039 3112
Size: 2431 496 3413
Fragments arranged by size:
3413 2431 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2907 3546 1163
Size: 573 1171 639 3957
Fragments arranged by size:
3957 1171 639 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3790 4551 5579 1540
Size: 275 186 1789 761 1028 2301
Fragments arranged by size:
2301 1789 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2247 4401 4486 5557 2247
Size: 2154 85 1071 3030
Fragments arranged by size:
3030 2154 1071 85
BsmI GAATG_Cn'
Cuts at: 3851 3944 3851
Size: 93 6247
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2247 3659 4401 4486 5557 1391
Size: 399 457 1412 742 85 1071 2174
Fragments arranged by size:
2174 1412 1071 742 457 399 85
BspEI T'CCGG_A
Cuts at: 6003 6003
Size: 6340
BspHI T'CATG_A
Cuts at: 2779 4229 5959 2779
Size: 1450 1730 3160
Fragments arranged by size:
3160 1730 1450
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3695 844
Size: 2851 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4227 1394
Size: 429 2404 3507
Fragments arranged by size:
3507 2404 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2763 3405 4835 5009 65
Size: 1183 1515 642 1430 174 1396
Fragments arranged by size:
1515 1430 1396 1183 642 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4984 5171 1700
Size: 110 3174 187 2869
Fragments arranged by size:
3174 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6340
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6340
BssSI C'ACGA_G
Cuts at: 4400 5412 4400
Size: 1012 5328
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6340
BstBI TT'CG_AA
Cuts at: 5144 5144
Size: 6340
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6340
BstXI CCAn_nnnn'nTGG
Cuts at: 3394 3394
Size: 6340
BstYI r'GATC_y
Cuts at: 1972 2956 3670 4037 4422 4439 5891 5977 5989
Size: 984 714 367 385 17 1452 86 12
Cuts at: 5989 6335 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 984 714 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2340 2340
Size: 6340
BtgI C'CryG_G
Cuts at: 513 1065 2004 3147 3237 513
Size: 552 939 1143 90 3616
Fragments arranged by size:
3616 1143 939 552 90
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5601
Fragments arranged by size:
5601 683 56
BtsI GCAGTG_nn'
Cuts at: 3492 3856 4650 4670 3492
Size: 364 794 20 5162
Fragments arranged by size:
5162 794 364 20
ClaI AT'CG_AT
Cuts at: 1554 2208 3267 1554
Size: 654 1059 4627
Fragments arranged by size:
4627 1059 654
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2008 2043 3222 3257 529
Size: 35 1444 35 1179 35 3612
Fragments arranged by size:
3612 1444 1179 35 35 35
DraI TTT'AAA
Cuts at: 3644 3644
Size: 6340
DraIII CAC_nnn'GTG
Cuts at: 2881 3221 3569 2881
Size: 340 348 5652
Fragments arranged by size:
5652 348 340
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5347 809
Size: 507 4031 1802
Fragments arranged by size:
4031 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3787 4676 6305
Size: 54 1425 50 275 18 1957 889 1629
Cuts at: 6305 8
Size: 43
Fragments arranged by size:
1957 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3787 1537
Size: 275 1975 4090
Fragments arranged by size:
4090 1975 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3300 3491 4270 1353
Size: 1947 191 779 3423
Fragments arranged by size:
3423 1947 779 191
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4927 5301 5447 387
Size: 1064 138 3338 374 146 1280
Fragments arranged by size:
3338 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2245 2245
Size: 6340
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2256 2298 2552 2971 3072 3515 3633
Size: 393 624 42 254 419 101 443 118
Cuts at: 3633 4403 5000 5787 6055 1239
Size: 770 597 787 268 1524
Fragments arranged by size:
1524 787 770 624 597 443 419 393
268 254 118 101 42
EcoNI CCTnn'n_nnAGG
Cuts at: 3158 3158
Size: 6340
EcoO109I rG'GnC_Cy
Cuts at: 3456 3456
Size: 6340
EcoRI G'AATT_C
Cuts at: 3649 4027 3649
Size: 378 5962
FspI TGC'GCA
Cuts at: 4846 4846
Size: 6340
HaeII r_GCGC'y
Cuts at: 1678 2095 5487 1678
Size: 417 3392 2531
Fragments arranged by size:
3392 2531 417
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2468 2500 3215 3247 4981
Size: 32 135 32 896 32 715 32 1734
Cuts at: 4981 5013 5055 5087 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1734 896 715 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2146 2155 3234 3684 3930 669
Size: 988 281 208 9 1079 450 246 3079
Fragments arranged by size:
3079 1079 988 450 281 246 208 9
HindIII A'AGCT_T
Cuts at: 748 2619 748
Size: 1871 4469
HpaI GTT'AAC
Cuts at: 2146 3930 2146
Size: 1784 4556
KpnI G_GTAC'C
Cuts at: 3665 3665
Size: 6340
MfeI C'AATT_G
Cuts at: 3939 3939
Size: 6340
MluI A'CGCG_T
Cuts at: 6216 6216
Size: 6340
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5454 5638 1305
Size: 4149 184 2007
Fragments arranged by size:
4149 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4518
Fragments arranged by size:
4518 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2784 2883 3392 4298 4657 4816 518
Size: 648 1618 99 509 906 359 159 2042
Fragments arranged by size:
2042 1618 906 648 509 359 159 99
MspA1I CmG'CkG
Cuts at: 1606 2128 2450 2678 3756 4433 5581 5826 1606
Size: 522 322 228 1078 677 1148 245 2120
Fragments arranged by size:
2120 1148 1078 677 522 322 245 228
NcoI C'CATG_G
Cuts at: 513 1065 2004 3147 3237 513
Size: 552 939 1143 90 3616
Fragments arranged by size:
3616 1143 939 552 90
NdeI CA'TA_TG
Cuts at: 387 2424 387
Size: 2037 4303
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6340
NotI GC'GGCC_GC
Cuts at: 3787 3787
Size: 6340
NspI r_CATG'y
Cuts at: 1132 1229 2375 3001 3698 5243 1132
Size: 97 1146 626 697 1545 2229
Fragments arranged by size:
2229 1545 1146 697 626 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6340
PciI A'CATG_T
Cuts at: 2997 5239 2997
Size: 2242 4098
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3123 1108
Size: 2015 4325
PfoI T'CCnGG_A
Cuts at: 2283 3122 2283
Size: 839 5501
PmeI GTTT'AAAC
Cuts at: 3644 3644
Size: 6340
PmlI CAC'GTG
Cuts at: 2881 2881
Size: 6340
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4409 4441 5922 5954 4409
Size: 32 1481 32 4795
Fragments arranged by size:
4795 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3456 3456
Size: 6340
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6340
PsiI TTA'TAA
Cuts at: 3910 3910
Size: 6340
PstI C_TGCA'G
Cuts at: 830 3177 3692 830
Size: 2347 515 3478
Fragments arranged by size:
3478 2347 515
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6340
PvuII CAG'CTG
Cuts at: 2128 2450 2678 2128
Size: 322 228 5790
Fragments arranged by size:
5790 322 228
SacI G_AGCT'C
Cuts at: 2247 2247
Size: 6340
SalI G'TCGA_C
Cuts at: 1936 2153 3682 1936
Size: 217 1529 4594
Fragments arranged by size:
4594 1529 217
SbfI CC_TGCA'GG
Cuts at: 3177 3692 3177
Size: 515 5825
ScaI AGT'ACT
Cuts at: 4588 6300 4588
Size: 1712 4628
SexAI A'CCwGG_T
Cuts at: 3513 3513
Size: 6340
SfcI C'TryA_G
Cuts at: 826 1045 2991 3173 3688 4823 5504 5695 826
Size: 219 1946 182 515 1135 681 191 1471
Fragments arranged by size:
1946 1471 1135 681 515 219 191 182
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6340
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6340
SmaI CCC'GGG
Cuts at: 1872 3667 1872
Size: 1795 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2867 4444 5345 5607 820
Size: 197 926 924 1577 901 262 1553
Fragments arranged by size:
1577 1553 926 924 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4131 493
Size: 3638 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6340
SphI G_CATG'C
Cuts at: 1132 2375 3698 1132
Size: 1243 1323 3774
Fragments arranged by size:
3774 1323 1243
SspI AAT'ATT
Cuts at: 5 52 2170 4264 5
Size: 47 2118 2094 2081
Fragments arranged by size:
2118 2094 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6340
StyI C'CwwG_G
Cuts at: 513 1065 2004 3147 3237 3779 513
Size: 552 939 1143 90 542 3074
Fragments arranged by size:
3074 1143 939 552 542 90
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3126 4367 4384 4537 4722 5167 325
Size: 1255 1546 1241 17 153 185 445 1498
Fragments arranged by size:
1546 1498 1255 1241 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2643 4586 6298
Size: 274 80 33 51 1443 666 1943 1712
Cuts at: 6298 96
Size: 138
Fragments arranged by size:
1943 1712 1443 666 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2379 2411 6300 6332 2379
Size: 32 3889 32 2387
Fragments arranged by size:
3889 2387 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2267 2827 1316
Size: 951 560 4829
Fragments arranged by size:
4829 951 560
XbaI T'CTAG_A
Cuts at: 3676 3676
Size: 6340
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3120 3154 3120
Size: 34 6306
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6340
XmaI C'CCGG_G
Cuts at: 1870 3665 1870
Size: 1795 4545
XmnI GAAnn'nnTTC
Cuts at: 3500 4469 3500
Size: 969 5371
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4148 276
Size: 53 83 186 936 2614 2468
Fragments arranged by size:
2614 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PspOMI PspXI PsrI RsrII SacII SanDI SapI
SfiI SfoI SrfI SwaI