Restriction map for
pFN21AB3113
(Circular) (Six-base) MAPSORT of: pFN21AB3113.seq Check: 7654 from: 1 to: 5674
pFN21AB3113 5674 bp
With 182 enzymes: SgfI *
October 20, 2009 10:53 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3484 278
Size: 53 83 186 936 1948 2468
Fragments arranged by size:
2468 1948 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3017 1937
Size: 1080 4594
Acc65I G'GTAC_C
Cuts at: 2995 2995
Size: 5674
AclI AA'CG_TT
Cuts at: 1563 3801 4174 1563
Size: 2238 373 3063
Fragments arranged by size:
3063 2238 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2505 2869 2876 3737 5121 5389 1632
Size: 873 364 7 861 1384 268 1917
Fragments arranged by size:
1917 1384 873 861 364 268 7
AflII C'TTAA_G
Cuts at: 820 1017 2686 820
Size: 197 1669 3808
Fragments arranged by size:
3808 1669 197
AflIII A'CryG_T
Cuts at: 1203 2245 4573 5550 1203
Size: 1042 2328 977 1327
Fragments arranged by size:
2328 1327 1042 977
AgeI A'CCGG_T
Cuts at: 4505 4505
Size: 5674
AhdI GACnn_n'nnGTC
Cuts at: 4403 4403
Size: 5674
AleI CACnn'nnGTG
Cuts at: 1166 2906 1166
Size: 1740 3934
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5674
AlwNI CAG_nnn'CTG
Cuts at: 2542 3417 4989 2542
Size: 875 1572 3227
Fragments arranged by size:
3227 1572 875
ApaLI G'TGCA_C
Cuts at: 2449 3731 4887 2449
Size: 1282 1156 3236
Fragments arranged by size:
3236 1282 1156
ApoI r'AATT_y
Cuts at: 1493 2638 2750 2983 3209 3361 5470 1493
Size: 1145 112 233 226 152 2109 1697
Fragments arranged by size:
2109 1697 1145 233 226 152 112
AseI AT'TA_AT
Cuts at: 160 1032 4228 160
Size: 872 3196 1606
Fragments arranged by size:
3196 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5674
AvaI C'yCGr_G
Cuts at: 1870 1943 2999 1870
Size: 73 1056 4545
Fragments arranged by size:
4545 1056 73
BamHI G'GATC_C
Cuts at: 2158 2425 3004 3371 2158
Size: 267 579 367 4461
Fragments arranged by size:
4461 579 367 267
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2995 4450 618
Size: 325 217 627 1208 1455 1842
Fragments arranged by size:
1842 1455 1208 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2900 2993 1391
Size: 1509 93 4072
Fragments arranged by size:
4072 1509 93
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2882 928
Size: 971 983 3720
Fragments arranged by size:
3720 983 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3865 3899 1903
Size: 34 1928 34 3678
Fragments arranged by size:
3678 1928 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3566 4776 3566
Size: 1210 4464
BclI T'GATC_A
Cuts at: 1548 2533 1548
Size: 985 4689
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4285 136
Size: 107 122 71 631 605 2613 1525
Fragments arranged by size:
2613 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2940 5669 2940
Size: 2729 2945
BlpI GC'TnA_GC
Cuts at: 3091 3091
Size: 5674
Bme1580I G_kGCm'C
Cuts at: 1790 2453 3735 4891 1790
Size: 663 1282 1156 2573
Fragments arranged by size:
2573 1282 1156 663
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5674
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3405 4363 442
Size: 416 53 83 399 24 1988 958 1753
Fragments arranged by size:
1988 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5674
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2919 2919
Size: 5674
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4334 1239
Size: 3095 2579
Bpu10I CC'TnA_GC
Cuts at: 2177 2337 2177
Size: 160 5514
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2629 3799 4664 4962 2629
Size: 1170 865 298 3341
Fragments arranged by size:
3341 1170 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2499 4337 882
Size: 617 1000 1838 2219
Fragments arranged by size:
2219 1838 1000 617
BsaAI yAC'GTr
Cuts at: 493 1204 3465 493
Size: 711 2261 2702
Fragments arranged by size:
2702 2261 711
BsaBI GATnn'nnATC
Cuts at: 5668 5668
Size: 5674
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2045 2053 3481 3863
Size: 53 83 186 936 512 8 1428 382
Cuts at: 3863 4498 275
Size: 635 1451
Fragments arranged by size:
1451 1428 936 635 512 382 186 83
53 8
BsaWI w'CCGG_w
Cuts at: 4107 4505 4779 4926 5337 4107
Size: 398 274 147 411 4444
Fragments arranged by size:
4444 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5449
Fragments arranged by size:
5449 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5674
BseYI C'CCAG_C
Cuts at: 2171 2679 4877 5373 2171
Size: 508 2198 496 2472
Fragments arranged by size:
2472 2198 508 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2574 1163
Size: 573 838 4263
Fragments arranged by size:
4263 838 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3124 3885 4913 1540
Size: 275 186 1123 761 1028 2301
Fragments arranged by size:
2301 1123 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2453 3735 3820 4891 2453
Size: 1282 85 1071 3236
Fragments arranged by size:
3236 1282 1071 85
BsmI GAATG_Cn'
Cuts at: 3185 3278 3185
Size: 93 5581
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2453 2900 2993 3735 3820 4891 1391
Size: 399 663 447 93 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 663 447 399 93 85
BspEI T'CCGG_A
Cuts at: 5337 5337
Size: 5674
BspHI T'CATG_A
Cuts at: 3563 5293 3563
Size: 1730 3944
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2094 3029 844
Size: 1250 935 3489
Fragments arranged by size:
3489 1250 935
BsrBI CCG'CTC
Cuts at: 1394 1823 2027 3561 1394
Size: 429 204 1534 3507
Fragments arranged by size:
3507 1534 429 204
BsrDI GCAATG_nn'
Cuts at: 65 1248 4169 4343 65
Size: 1183 2921 174 1396
Fragments arranged by size:
2921 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4318 4505 1700
Size: 110 2508 187 2869
Fragments arranged by size:
2869 2508 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5674
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5674
BssSI C'ACGA_G
Cuts at: 3734 4746 3734
Size: 1012 4662
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5674
BstBI TT'CG_AA
Cuts at: 4478 4478
Size: 5674
BstEII G'GTnAC_C
Cuts at: 1184 2647 1184
Size: 1463 4211
BstXI CCAn_nnnn'nTGG
Cuts at: 2507 2908 2507
Size: 401 5273
BstYI r'GATC_y
Cuts at: 1972 2158 2425 2940 3004 3371 3756 3773 5225
Size: 186 267 515 64 367 385 17 1452
Cuts at: 5225 5311 5323 5669 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 515 385 367 346 267 186
86 64 17 12
Bsu36I CC'TnA_GG
Cuts at: 2527 2527
Size: 5674
BtgI C'CryG_G
Cuts at: 513 1065 2056 513
Size: 552 991 4131
Fragments arranged by size:
4131 991 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2113 2283 487
Size: 683 56 887 170 3878
Fragments arranged by size:
3878 887 683 170 56
BtsI GCAGTG_nn'
Cuts at: 2292 2312 3190 3984 4004 2292
Size: 20 878 794 20 3962
Fragments arranged by size:
3962 878 794 20 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5674
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5639
DraI TTT'AAA
Cuts at: 2978 2978
Size: 5674
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4681 809
Size: 507 3365 1802
Fragments arranged by size:
3365 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3121 4010 5639
Size: 54 1425 50 275 18 1291 889 1629
Cuts at: 5639 8
Size: 43
Fragments arranged by size:
1629 1425 1291 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3121 1537
Size: 275 1309 4090
Fragments arranged by size:
4090 1309 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2841 3604 1353
Size: 1488 763 3423
Fragments arranged by size:
3423 1488 763
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4261 4635 4781 387
Size: 1064 138 2672 374 146 1280
Fragments arranged by size:
2672 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2505 2869 2876 3737 4334 5121 5389
Size: 393 873 364 7 861 597 787 268
Cuts at: 5389 1239
Size: 1524
Fragments arranged by size:
1524 873 861 787 597 393 364 268
7
EcoRI G'AATT_C
Cuts at: 2983 3361 2983
Size: 378 5296
FspI TGC'GCA
Cuts at: 4180 4180
Size: 5674
HaeII r_GCGC'y
Cuts at: 1678 4821 1678
Size: 3143 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2562 2594 4315 4347 4389
Size: 32 135 32 990 32 1721 32 42
Cuts at: 4389 4421 1373
Size: 32 2626
Fragments arranged by size:
2626 1721 990 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2662 3018 3264 669
Size: 988 281 724 356 246 3079
Fragments arranged by size:
3079 988 724 356 281 246
HindIII A'AGCT_T
Cuts at: 748 2191 748
Size: 1443 4231
HpaI GTT'AAC
Cuts at: 3264 3264
Size: 5674
KpnI G_GTAC'C
Cuts at: 2999 2999
Size: 5674
MfeI C'AATT_G
Cuts at: 3273 3273
Size: 5674
MluI A'CGCG_T
Cuts at: 5550 5550
Size: 5674
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2416 2453 4788 4972 1305
Size: 1111 37 2335 184 2007
Fragments arranged by size:
2335 2007 1111 184 37
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3852
Fragments arranged by size:
3852 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2730 2906 3632 3991 4150 518
Size: 648 1564 176 726 359 159 2042
Fragments arranged by size:
2042 1564 726 648 359 176 159
MspA1I CmG'CkG
Cuts at: 1606 3090 3767 4915 5160 1606
Size: 1484 677 1148 245 2120
Fragments arranged by size:
2120 1484 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5122
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5674
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5674
NotI GC'GGCC_GC
Cuts at: 3121 3121
Size: 5674
NspI r_CATG'y
Cuts at: 1132 1229 2249 3032 4577 1132
Size: 97 1020 783 1545 2229
Fragments arranged by size:
2229 1545 1020 783 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5674
PciI A'CATG_T
Cuts at: 2245 4573 2245
Size: 2328 3346
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2435 1108
Size: 1327 4347
PmeI GTTT'AAAC
Cuts at: 2978 2978
Size: 5674
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2235 2267 3743 3775 5256 5288 2235
Size: 32 1476 32 1481 32 2621
Fragments arranged by size:
2621 1481 1476 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2886 1613
Size: 1273 4401
PsiI TTA'TAA
Cuts at: 3244 3244
Size: 5674
PstI C_TGCA'G
Cuts at: 830 2283 2308 3026 830
Size: 1453 25 718 3478
Fragments arranged by size:
3478 1453 718 25
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5674
SalI G'TCGA_C
Cuts at: 1936 3016 1936
Size: 1080 4594
SbfI CC_TGCA'GG
Cuts at: 3026 3026
Size: 5674
ScaI AGT'ACT
Cuts at: 3922 5634 3922
Size: 1712 3962
SfcI C'TryA_G
Cuts at: 826 1045 2260 2279 2304 2665 3022 4157 4838
Size: 219 1215 19 25 361 357 1135 681
Cuts at: 4838 5029 826
Size: 191 1471
Fragments arranged by size:
1471 1215 1135 681 361 357 219 191
25 19
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5674
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5674
SmaI CCC'GGG
Cuts at: 1872 3001 1872
Size: 1129 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2608 2686 3778 4679 4941 820
Size: 197 926 665 78 1092 901 262 1553
Fragments arranged by size:
1553 1092 926 901 665 262 197 78
SnaBI TAC'GTA
Cuts at: 493 3465 493
Size: 2972 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5674
SphI G_CATG'C
Cuts at: 1132 3032 1132
Size: 1900 3774
SspI AAT'ATT
Cuts at: 5 52 2154 3598 5
Size: 47 2102 1444 2081
Fragments arranged by size:
2102 2081 1444 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5674
StyI C'CwwG_G
Cuts at: 513 1065 3113 513
Size: 552 2048 3074
Fragments arranged by size:
3074 2048 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3701 3718 3871 4056 4501 325
Size: 1255 2121 17 153 185 445 1498
Fragments arranged by size:
2121 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3920 5632 96
Size: 274 80 33 51 1443 1943 1712 138
Fragments arranged by size:
1943 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5634 5666 5634
Size: 32 5642
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5674
XbaI T'CTAG_A
Cuts at: 3010 3010
Size: 5674
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5674
XmaI C'CCGG_G
Cuts at: 1870 2999 1870
Size: 1129 4545
XmnI GAAnn'nnTTC
Cuts at: 3803 3803
Size: 5674
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3482 276
Size: 53 83 186 936 1948 2468
Fragments arranged by size:
2468 1948 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI
EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI
PspXI PsrI PvuII RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI XcmI