Restriction map for
pFN21AB3104
(Circular) (Six-base) MAPSORT of: pFN21AB3104.seq Check: 4015 from: 1 to: 7114
pFN21AB3104 7114 bp
With 182 enzymes: SgfI *
October 20, 2009 10:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4028 4028
Size: 7114
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4924 278
Size: 53 83 186 936 3388 2468
Fragments arranged by size:
3388 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2462 4457 1937
Size: 525 1995 4594
Fragments arranged by size:
4594 1995 525
Acc65I G'GTAC_C
Cuts at: 4435 4435
Size: 7114
AclI AA'CG_TT
Cuts at: 1563 5241 5614 1563
Size: 3678 373 3063
Fragments arranged by size:
3678 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2212 2528 3418 3430 3671 3926 4342 5177
Size: 580 316 890 12 241 255 416 835
Cuts at: 5177 6561 6829 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1384 890 835 580 416 316 268
255 241 12
AfeI AGC'GCT
Cuts at: 2029 3439 2029
Size: 1410 5704
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6917
AflIII A'CryG_T
Cuts at: 1203 2617 2887 3030 3559 6013 6990 1203
Size: 1414 270 143 529 2454 977 1327
Fragments arranged by size:
2454 1414 1327 977 529 270 143
AgeI A'CCGG_T
Cuts at: 4326 5945 4326
Size: 1619 5495
AhdI GACnn_n'nnGTC
Cuts at: 5843 5843
Size: 7114
AleI CACnn'nnGTG
Cuts at: 1166 2731 2972 1166
Size: 1565 241 5308
Fragments arranged by size:
5308 1565 241
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7114
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4152 4184 4152
Size: 32 7082
AlwNI CAG_nnn'CTG
Cuts at: 2065 2688 3649 4171 4321 4857 6429 2065
Size: 623 961 522 150 536 1572 2750
Fragments arranged by size:
2750 1572 961 623 536 522 150
ApaI G_GGCC'C
Cuts at: 2564 2564
Size: 7114
ApaLI G'TGCA_C
Cuts at: 2613 3509 5171 6327 2613
Size: 896 1662 1156 3400
Fragments arranged by size:
3400 1662 1156 896
ApoI r'AATT_y
Cuts at: 1493 2244 2282 2487 3194 4200 4423 4649 4801
Size: 751 38 205 707 1006 223 226 152
Cuts at: 4801 6910 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1006 751 707 226 223 205
152 38
AseI AT'TA_AT
Cuts at: 160 1032 5668 160
Size: 872 4636 1606
Fragments arranged by size:
4636 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7114
AvaI C'yCGr_G
Cuts at: 1870 1943 2255 3712 4439 1870
Size: 73 312 1457 727 4545
Fragments arranged by size:
4545 1457 727 312 73
BamHI G'GATC_C
Cuts at: 2500 4362 4444 4811 2500
Size: 1862 82 367 4803
Fragments arranged by size:
4803 1862 367 82
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3707 4435 5890 618
Size: 325 217 627 1920 728 1455 1842
Fragments arranged by size:
1920 1842 1455 728 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2564 2802 4433 1391
Size: 1173 238 1631 4072
Fragments arranged by size:
4072 1631 1173 238
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2241 2720 928
Size: 971 342 479 5322
Fragments arranged by size:
5322 971 479 342
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2737 2771 2980 3014 5305 5339 1903
Size: 34 800 34 209 34 2291 34 3678
Fragments arranged by size:
3678 2291 800 209 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5006 6216 5006
Size: 1210 5904
BclI T'GATC_A
Cuts at: 1548 4173 1548
Size: 2625 4489
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2568 5725 136
Size: 107 122 71 631 605 896 3157 1525
Fragments arranged by size:
3157 1525 896 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2221 3315 7109 2221
Size: 1094 3794 2226
Fragments arranged by size:
3794 2226 1094
BlpI GC'TnA_GC
Cuts at: 4531 4531
Size: 7114
Bme1580I G_kGCm'C
Cuts at: 1790 2564 2617 3513 5175 6331 1790
Size: 774 53 896 1662 1156 2573
Fragments arranged by size:
2573 1662 1156 896 774 53
BmgBI CAC'GTC
Cuts at: 1318 3277 1318
Size: 1959 5155
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3280 4845 5803
Size: 416 53 83 399 24 1863 1565 958
Cuts at: 5803 442
Size: 1753
Fragments arranged by size:
1863 1753 1565 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7114
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2097 2097
Size: 7114
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2388 2942 3887 4288 5774 1239
Size: 1149 554 945 401 1486 2579
Fragments arranged by size:
2579 1486 1149 945 554 401
Bpu10I CC'TnA_GC
Cuts at: 2480 3326 3968 2480
Size: 846 642 5626
Fragments arranged by size:
5626 846 642
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3458 5239 6104 6402 3458
Size: 1781 865 298 4170
Fragments arranged by size:
4170 1781 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2650 2742 5777 882
Size: 617 1151 92 3035 2219
Fragments arranged by size:
3035 2219 1151 617 92
BsaAI yAC'GTr
Cuts at: 493 1204 4905 493
Size: 711 3701 2702
Fragments arranged by size:
3701 2702 711
BsaBI GATnn'nnATC
Cuts at: 7108 7108
Size: 7114
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3054 4921 5303 5938
Size: 53 83 186 936 1521 1867 382 635
Cuts at: 5938 275
Size: 1451
Fragments arranged by size:
1867 1521 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2740 4326 5547 5945 6219 6366 6777 2740
Size: 1586 1221 398 274 147 411 3077
Fragments arranged by size:
3077 1586 1221 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2801 2831 1178
Size: 30 165 30 1398 30 5461
Fragments arranged by size:
5461 1398 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2717 3884 4418 4421 1186
Size: 1531 1167 534 3 3879
Fragments arranged by size:
3879 1531 1167 534 3
BseYI C'CCAG_C
Cuts at: 2873 3222 3750 3856 3985 6317 6813 2873
Size: 349 528 106 129 2332 496 3174
Fragments arranged by size:
3174 2332 528 496 349 129 106
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2066 3055 3409 3821 1163
Size: 573 330 989 354 412 4456
Fragments arranged by size:
4456 989 573 412 354 330
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3805 4564 5325 6353 1540
Size: 275 186 1804 759 761 1028 2301
Fragments arranged by size:
2301 1804 1028 761 759 275 186
BsiHKAI G_wGCw'C
Cuts at: 2617 2802 3332 3513 3974 5175 5260 6331 2617
Size: 185 530 181 461 1201 85 1071 3400
Fragments arranged by size:
3400 1201 1071 530 461 185 181 85
BsmI GAATG_Cn'
Cuts at: 4358 4625 4718 4358
Size: 267 93 6754
Fragments arranged by size:
6754 267 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3343 3749 3343
Size: 406 6708
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2564 2617 2802 3332 3513 3974 4433
Size: 399 774 53 185 530 181 461 459
Cuts at: 4433 5175 5260 6331 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 774 742 530 461 459 399
185 181 85 53
BspEI T'CCGG_A
Cuts at: 2740 6777 2740
Size: 4037 3077
BspHI T'CATG_A
Cuts at: 2248 5003 6733 2248
Size: 2755 1730 2629
Fragments arranged by size:
2755 2629 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3348 3642 4028 4469 844
Size: 2504 294 386 441 3489
Fragments arranged by size:
3489 2504 441 386 294
BsrBI CCG'CTC
Cuts at: 1394 1823 5001 1394
Size: 429 3178 3507
Fragments arranged by size:
3507 3178 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2539 5609 5783 65
Size: 1183 1291 3070 174 1396
Fragments arranged by size:
3070 1396 1291 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2813 4326 5758 5945 1700
Size: 110 1003 1513 1432 187 2869
Fragments arranged by size:
2869 1513 1432 1003 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7114
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7114
BssSI C'ACGA_G
Cuts at: 2749 3083 5174 6186 2749
Size: 334 2091 1012 3677
Fragments arranged by size:
3677 2091 1012 334
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7114
BstBI TT'CG_AA
Cuts at: 5918 5918
Size: 7114
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7114
BstXI CCAn_nnnn'nTGG
Cuts at: 2641 3376 2641
Size: 735 6379
BstYI r'GATC_y
Cuts at: 1972 2221 2500 3285 3315 4362 4444 4811 5196
Size: 249 279 785 30 1047 82 367 385
Cuts at: 5196 5213 6665 6751 6763 7109 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1047 785 385 367 346 279
249 86 82 30 17 12
BstZ17I GTA'TAC
Cuts at: 2463 2463
Size: 7114
BtgI C'CryG_G
Cuts at: 513 1065 2945 2970 3060 3775 3805 4096 513
Size: 552 1880 25 90 715 30 291 3531
Fragments arranged by size:
3531 1880 715 552 291 90 30 25
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2707 487
Size: 683 56 1481 4894
Fragments arranged by size:
4894 1481 683 56
BtsI GCAGTG_nn'
Cuts at: 2114 3401 4185 4630 5424 5444 2114
Size: 1287 784 445 794 20 3784
Fragments arranged by size:
3784 1287 794 784 445 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7114
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7079
DraI TTT'AAA
Cuts at: 4418 4418
Size: 7114
DraIII CAC_nnn'GTG
Cuts at: 3844 3844
Size: 7114
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6121 809
Size: 507 4805 1802
Fragments arranged by size:
4805 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3145 3802 3936
Size: 54 1425 50 275 18 1315 657 134
Cuts at: 3936 4561 5450 7079 8
Size: 625 889 1629 43
Fragments arranged by size:
1629 1425 1315 889 657 625 275 134
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3802 4561 1537
Size: 275 1990 759 4090
Fragments arranged by size:
4090 1990 759 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2231 2694 5044 1353
Size: 878 463 2350 3423
Fragments arranged by size:
3423 2350 878 463
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5701 6075 6221 387
Size: 1064 138 4112 374 146 1280
Fragments arranged by size:
4112 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2800 2800
Size: 7114
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2212 2388 2528 2942 3418 3430 3671
Size: 393 580 176 140 414 476 12 241
Cuts at: 3671 3887 3926 4288 4342 5177 5774 6561 6829
Size: 216 39 362 54 835 597 787 268
Cuts at: 6829 1239
Size: 1524
Fragments arranged by size:
1524 835 787 597 580 476 414 393
362 268 241 216 176 140 54 39
12
EcoO109I rG'GnC_Cy
Cuts at: 2560 2561 3337 3485 2560
Size: 1 776 148 6189
Fragments arranged by size:
6189 776 148 1
EcoRI G'AATT_C
Cuts at: 2282 4423 4801 2282
Size: 2141 378 4595
Fragments arranged by size:
4595 2141 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3175 3337 3175
Size: 162 6952
FspI TGC'GCA
Cuts at: 5620 5620
Size: 7114
HaeII r_GCGC'y
Cuts at: 1678 2031 3441 6261 1678
Size: 353 1410 2820 2531
Fragments arranged by size:
2820 2531 1410 353
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2460 2492 3342 3374 4165
Size: 32 135 32 888 32 850 32 791
Cuts at: 4165 4197 5755 5787 5829 5861 1373
Size: 32 1558 32 42 32 2626
Fragments arranged by size:
2626 1558 888 850 791 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4458 4704 669
Size: 988 281 2520 246 3079
Fragments arranged by size:
3079 2520 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2540 748
Size: 1792 5322
HpaI GTT'AAC
Cuts at: 4704 4704
Size: 7114
KpnI G_GTAC'C
Cuts at: 4439 4439
Size: 7114
MfeI C'AATT_G
Cuts at: 2109 4713 2109
Size: 2604 4510
MluI A'CGCG_T
Cuts at: 6990 6990
Size: 7114
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6228 6412 1305
Size: 4923 184 2007
Fragments arranged by size:
4923 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5292
Fragments arranged by size:
5292 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2639 2731 2969 2972 3087 3209 5072
Size: 648 1473 92 238 3 115 122 1863
Cuts at: 5072 5431 5590 518
Size: 359 159 2042
Fragments arranged by size:
2042 1863 1473 648 359 238 159 122
115 92 3
MspA1I CmG'CkG
Cuts at: 1606 2177 2578 3813 4530 5207 6355 6600 1606
Size: 571 401 1235 717 677 1148 245 2120
Fragments arranged by size:
2120 1235 1148 717 677 571 401 245
NcoI C'CATG_G
Cuts at: 513 1065 2970 4096 513
Size: 552 1905 1126 3531
Fragments arranged by size:
3531 1905 1126 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7114
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7114
NotI GC'GGCC_GC
Cuts at: 4561 4561
Size: 7114
NspI r_CATG'y
Cuts at: 1132 1229 2621 3563 4472 6017 1132
Size: 97 1392 942 909 1545 2229
Fragments arranged by size:
2229 1545 1392 942 909 97
PasI CC'CwG_GG
Cuts at: 1348 3178 1348
Size: 1830 5284
PciI A'CATG_T
Cuts at: 2617 3559 6013 2617
Size: 942 2454 3718
Fragments arranged by size:
3718 2454 942
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2920 3457 1108
Size: 1812 537 4765
Fragments arranged by size:
4765 1812 537
PfoI T'CCnGG_A
Cuts at: 4365 4365
Size: 7114
PmeI GTTT'AAAC
Cuts at: 4418 4418
Size: 7114
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5183 5215 6696 6728 5183
Size: 32 1481 32 5569
Fragments arranged by size:
5569 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3337 3485 3337
Size: 148 6966
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7114
PsiI TTA'TAA
Cuts at: 4684 4684
Size: 7114
PspOMI G'GGCC_C
Cuts at: 2560 2560
Size: 7114
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3413 3445 3413
Size: 32 7082
PstI C_TGCA'G
Cuts at: 830 4466 830
Size: 3636 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7114
PvuII CAG'CTG
Cuts at: 2578 2578
Size: 7114
SacI G_AGCT'C
Cuts at: 2802 2802
Size: 7114
SalI G'TCGA_C
Cuts at: 1936 4456 1936
Size: 2520 4594
SbfI CC_TGCA'GG
Cuts at: 4466 4466
Size: 7114
ScaI AGT'ACT
Cuts at: 2960 5362 7074 2960
Size: 2402 1712 3000
Fragments arranged by size:
3000 2402 1712
SexAI A'CCwGG_T
Cuts at: 3411 3411
Size: 7114
SfcI C'TryA_G
Cuts at: 826 1045 2214 2290 3361 4462 5597 6278 6469
Size: 219 1169 76 1071 1101 1135 681 191
Cuts at: 6469 826
Size: 1471
Fragments arranged by size:
1471 1169 1135 1101 1071 681 219 191
76
SfiI GGCCn_nnn'nGGCC
Cuts at: 2568 2568
Size: 7114
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7114
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7114
SmaI CCC'GGG
Cuts at: 1872 3714 4441 1872
Size: 1842 727 4545
Fragments arranged by size:
4545 1842 727
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3473 5218 6119 6381 820
Size: 197 926 1530 1745 901 262 1553
Fragments arranged by size:
1745 1553 1530 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4905 493
Size: 4412 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7114
SphI G_CATG'C
Cuts at: 1132 4472 1132
Size: 3340 3774
SspI AAT'ATT
Cuts at: 5 52 5038 5
Size: 47 4986 2081
Fragments arranged by size:
4986 2081 47
StuI AGG'CCT
Cuts at: 1517 3101 1517
Size: 1584 5530
StyI C'CwwG_G
Cuts at: 513 1065 2016 2853 2970 2994 3138 3820 4096
Size: 552 951 837 117 24 144 682 276
Cuts at: 4096 4128 4553 513
Size: 32 425 3074
Fragments arranged by size:
3074 951 837 682 552 425 276 144
117 32 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2146 2927 3597 3692 5141 5158 5311
Size: 1255 566 781 670 95 1449 17 153
Cuts at: 5311 5496 5941 325
Size: 185 445 1498
Fragments arranged by size:
1498 1449 1255 781 670 566 445 185
153 95 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2958 3915 5360
Size: 274 80 33 51 1443 981 957 1445
Cuts at: 5360 7072 96
Size: 1712 138
Fragments arranged by size:
1712 1445 1443 981 957 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7074 7106 7074
Size: 32 7082
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7114
XbaI T'CTAG_A
Cuts at: 4450 4450
Size: 7114
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2917 2917
Size: 7114
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7114
XmaI C'CCGG_G
Cuts at: 1870 3712 4439 1870
Size: 1842 727 4545
Fragments arranged by size:
4545 1842 727
XmnI GAAnn'nnTTC
Cuts at: 4200 5243 4200
Size: 1043 6071
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4922 276
Size: 53 83 186 936 3388 2468
Fragments arranged by size:
3388 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PsrI PstI PvuI PvuII SacI SalI SbfI
ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI Bsu36I
EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PspXI RsrII SacII SanDI
SapI SfoI SrfI SwaI