Restriction map for
pFN21AB3100
(Circular) (Six-base) MAPSORT of: pFN21AB3100.seq Check: 2417 from: 1 to: 7273
pFN21AB3100 7273 bp
With 182 enzymes: SgfI *
February 27, 2009 14:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4357 4357
Size: 7273
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5083 278
Size: 53 83 186 936 3547 2468
Fragments arranged by size:
3547 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2825 4616 1937
Size: 888 1791 4594
Fragments arranged by size:
4594 1791 888
Acc65I G'GTAC_C
Cuts at: 3365 4594 3365
Size: 1229 6044
AclI AA'CG_TT
Cuts at: 1563 5400 5773 1563
Size: 3837 373 3063
Fragments arranged by size:
3837 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2042 2493 2622 5336 6720 6988 1632
Size: 410 451 129 2714 1384 268 1917
Fragments arranged by size:
2714 1917 1384 451 410 268 129
AfeI AGC'GCT
Cuts at: 3827 3887 3827
Size: 60 7213
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7076
AflIII A'CryG_T
Cuts at: 1203 6172 7149 1203
Size: 4969 977 1327
Fragments arranged by size:
4969 1327 977
AgeI A'CCGG_T
Cuts at: 6104 6104
Size: 7273
AhdI GACnn_n'nnGTC
Cuts at: 6002 6002
Size: 7273
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7273
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7273
AlwNI CAG_nnn'CTG
Cuts at: 2834 5016 6588 2834
Size: 2182 1572 3519
Fragments arranged by size:
3519 2182 1572
ApaLI G'TGCA_C
Cuts at: 5330 6486 5330
Size: 1156 6117
ApoI r'AATT_y
Cuts at: 1493 4582 4808 4960 7069 1493
Size: 3089 226 152 2109 1697
Fragments arranged by size:
3089 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5827 160
Size: 872 4795 1606
Fragments arranged by size:
4795 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7273
AvaI C'yCGr_G
Cuts at: 1870 1943 2070 2174 2246 2281 2679 3187 4598
Size: 73 127 104 72 35 398 508 1411
Cuts at: 4598 1870
Size: 4545
Fragments arranged by size:
4545 1411 508 398 127 104 73 72
35
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2331 2364 2619 2652 2331
Size: 33 255 33 6952
Fragments arranged by size:
6952 255 33 33
BamHI G'GATC_C
Cuts at: 4603 4970 4603
Size: 367 6906
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3236 3365 4594 6049 618
Size: 325 217 627 1449 129 1229 1455 1842
Fragments arranged by size:
1842 1455 1449 1229 627 325 217 129
BanII G_rGCy'C
Cuts at: 1391 2148 2213 2271 2743 3171 4078 4592 1391
Size: 757 65 58 472 428 907 514 4072
Fragments arranged by size:
4072 907 757 514 472 428 65 58
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2435 928
Size: 971 536 5766
Fragments arranged by size:
5766 971 536
BbvCI CC'TCA_GC
Cuts at: 2589 4431 2589
Size: 1842 5431
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2396 2430 5464 5498 1903
Size: 34 459 34 3034 34 3678
Fragments arranged by size:
3678 3034 459 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3239 5165 6375 3239
Size: 1926 1210 4137
Fragments arranged by size:
4137 1926 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7273
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2248 2497 5884
Size: 107 122 71 631 605 576 249 3387
Cuts at: 5884 136
Size: 1525
Fragments arranged by size:
3387 1525 631 605 576 249 122 107
71
BglII A'GATC_T
Cuts at: 7268 7268
Size: 7273
BlpI GC'TnA_GC
Cuts at: 2094 4690 2094
Size: 2596 4677
Bme1580I G_kGCm'C
Cuts at: 1790 2181 3239 5334 6490 1790
Size: 391 1058 2095 1156 2573
Fragments arranged by size:
2573 2095 1156 1058 391
BmgBI CAC'GTC
Cuts at: 1318 3025 1318
Size: 1707 5566
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2934 3454 5004
Size: 416 53 83 399 24 1517 520 1550
Cuts at: 5004 5962 442
Size: 958 1753
Fragments arranged by size:
1753 1550 1517 958 520 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7273
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2227 2821 2920 2227
Size: 594 99 6580
Fragments arranged by size:
6580 594 99
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2240 3930 5933 1239
Size: 1001 1690 2003 2579
Fragments arranged by size:
2579 2003 1690 1001
Bpu10I CC'TnA_GC
Cuts at: 2589 4431 2589
Size: 1842 5431
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2063 2471 4201 5398 6263 6561 2063
Size: 408 1730 1197 865 298 2775
Fragments arranged by size:
2775 1730 1197 865 408 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2192 2377 3553 5936 882
Size: 617 693 185 1176 2383 2219
Fragments arranged by size:
2383 2219 1176 693 617 185
BsaAI yAC'GTr
Cuts at: 493 1204 2845 5064 493
Size: 711 1641 2219 2702
Fragments arranged by size:
2702 2219 1641 711
BsaBI GATnn'nnATC
Cuts at: 2789 7267 2789
Size: 4478 2795
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2151 5080 5462 6097
Size: 53 83 186 936 618 2929 382 635
Cuts at: 6097 275
Size: 1451
Fragments arranged by size:
2929 1451 936 635 618 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5706 6104 6378 6525 6936 5706
Size: 398 274 147 411 6043
Fragments arranged by size:
6043 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4031 4061 1178
Size: 30 165 30 2628 30 4390
Fragments arranged by size:
4390 2628 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2057 2138 2204 2395 2577 3972 3987 1186
Size: 871 81 66 191 182 1395 15 4472
Fragments arranged by size:
4472 1395 871 191 182 81 66 15
BseYI C'CCAG_C
Cuts at: 2883 3432 3806 4017 4080 4128 4148 4436 4464
Size: 549 374 211 63 48 20 288 28
Cuts at: 4464 6476 6972 2883
Size: 2012 496 3184
Fragments arranged by size:
3184 2012 549 496 374 288 211 63
48 28 20
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6700
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4723 5484 6512 1540
Size: 275 186 2722 761 1028 2301
Fragments arranged by size:
2722 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2148 2452 2562 5334 5419 6490 2148
Size: 304 110 2772 85 1071 2931
Fragments arranged by size:
2931 2772 1071 304 110 85
BsmI GAATG_Cn'
Cuts at: 4784 4877 4784
Size: 93 7180
BsmBI CGTCTCn'nnnn_
Cuts at: 3541 3541
Size: 7273
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2148 2181 2213 2271 2452 2562 2743
Size: 399 358 33 32 58 181 110 181
Cuts at: 2743 3171 3239 4078 4592 5334 5419 6490 1391
Size: 428 68 839 514 742 85 1071 2174
Fragments arranged by size:
2174 1071 839 742 514 428 399 358
181 181 110 85 68 58 33 32
BspEI T'CCGG_A
Cuts at: 6936 6936
Size: 7273
BspHI T'CATG_A
Cuts at: 5162 6892 5162
Size: 1730 5543
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4357 4628 844
Size: 3513 271 3489
Fragments arranged by size:
3513 3489 271
BsrBI CCG'CTC
Cuts at: 1394 1823 2098 2140 2583 2704 5160 1394
Size: 429 275 42 443 121 2456 3507
Fragments arranged by size:
3507 2456 443 429 275 121 42
BsrDI GCAATG_nn'
Cuts at: 65 1248 3063 5768 5942 65
Size: 1183 1815 2705 174 1396
Fragments arranged by size:
2705 1815 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2946 3408 5917 6104 1700
Size: 110 1136 462 2509 187 2869
Fragments arranged by size:
2869 2509 1136 462 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7273
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7273
BssSI C'ACGA_G
Cuts at: 5333 6345 5333
Size: 1012 6261
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7273
BstBI TT'CG_AA
Cuts at: 6077 6077
Size: 7273
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7273
BstYI r'GATC_y
Cuts at: 1972 4603 4970 5355 5372 6824 6910 6922 7268
Size: 2631 367 385 17 1452 86 12 346
Cuts at: 7268 1972
Size: 1977
Fragments arranged by size:
2631 1977 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2056 2304 3862 3919 513
Size: 552 939 52 248 1558 57 3867
Fragments arranged by size:
3867 1558 939 552 248 57 52
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6534
Fragments arranged by size:
6534 683 56
BtsI GCAGTG_nn'
Cuts at: 3144 4475 4789 5583 5603 3144
Size: 1331 314 794 20 4814
Fragments arranged by size:
4814 1331 794 314 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7273
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3823 3858 529
Size: 35 3259 35 3944
Fragments arranged by size:
3944 3259 35 35
DraI TTT'AAA
Cuts at: 4577 4577
Size: 7273
DraIII CAC_nnn'GTG
Cuts at: 3462 4556 3462
Size: 1094 6179
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6280 809
Size: 507 4964 1802
Fragments arranged by size:
4964 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3427 4502 4720
Size: 54 1425 50 275 18 1597 1075 218
Cuts at: 4720 5609 7238 8
Size: 889 1629 43
Fragments arranged by size:
1629 1597 1425 1075 889 275 218 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4720 1537
Size: 275 2908 4090
Fragments arranged by size:
4090 2908 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2598 2889 5203 1353
Size: 1245 291 2314 3423
Fragments arranged by size:
3423 2314 1245 291
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4098 5860 6234 6380 387
Size: 1064 138 2509 1762 374 146 1280
Fragments arranged by size:
2509 1762 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2146 2146
Size: 7273
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2042 2240 2493 2622 3930 5336 5933
Size: 393 410 198 253 129 1308 1406 597
Cuts at: 5933 6720 6988 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1406 1308 787 597 410 393 268
253 198 129
EcoNI CCTnn'n_nnAGG
Cuts at: 3728 3728
Size: 7273
EcoO109I rG'GnC_Cy
Cuts at: 3103 3613 3902 3103
Size: 510 289 6474
Fragments arranged by size:
6474 510 289
EcoRI G'AATT_C
Cuts at: 4582 4960 4582
Size: 378 6895
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3106 3106
Size: 7273
FspI TGC'GCA
Cuts at: 3136 5779 3136
Size: 2643 4630
HaeII r_GCGC'y
Cuts at: 1678 2056 3829 3889 4224 6420 1678
Size: 378 1773 60 335 2196 2531
Fragments arranged by size:
2531 2196 1773 378 335 60
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4414 4446 5914 5946 5988
Size: 32 135 32 2842 32 1468 32 42
Cuts at: 5988 6020 1373
Size: 32 2626
Fragments arranged by size:
2842 2626 1468 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4617 4863 669
Size: 988 281 2679 246 3079
Fragments arranged by size:
3079 2679 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2475 748
Size: 1727 5546
HpaI GTT'AAC
Cuts at: 4863 4863
Size: 7273
KpnI G_GTAC'C
Cuts at: 3369 4598 3369
Size: 1229 6044
MfeI C'AATT_G
Cuts at: 2709 4872 2709
Size: 2163 5110
MluI A'CGCG_T
Cuts at: 7149 7149
Size: 7273
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2094 3223 3360 4074 6387 6571 1305
Size: 789 1129 137 714 2313 184 2007
Fragments arranged by size:
2313 2007 1129 789 714 184 137
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5451
Fragments arranged by size:
5451 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2728 5231 5590 5749 518
Size: 648 843 719 2503 359 159 2042
Fragments arranged by size:
2503 2042 843 719 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2058 3415 3432 3754 3864 3951 3990 4017
Size: 452 1357 17 322 110 87 39 27
Cuts at: 4017 4689 5366 6514 6759 1606
Size: 672 677 1148 245 2120
Fragments arranged by size:
2120 1357 1148 677 672 452 322 245
110 87 39 27 17
NaeI GCC'GGC
Cuts at: 3410 3410
Size: 7273
NcoI C'CATG_G
Cuts at: 513 1065 2004 2304 513
Size: 552 939 300 5482
Fragments arranged by size:
5482 939 552 300
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7273
NgoMIV G'CCGG_C
Cuts at: 3408 3408
Size: 7273
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7273
NotI GC'GGCC_GC
Cuts at: 4720 4720
Size: 7273
NruI TCG'CGA
Cuts at: 2040 2040
Size: 7273
NspI r_CATG'y
Cuts at: 1132 1229 3329 4631 6176 1132
Size: 97 2100 1302 1545 2229
Fragments arranged by size:
2229 2100 1545 1302 97
PasI CC'CwG_GG
Cuts at: 1348 4175 1348
Size: 2827 4446
PciI A'CATG_T
Cuts at: 6172 6172
Size: 7273
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3713 1108
Size: 2605 4668
PfoI T'CCnGG_A
Cuts at: 3723 4541 3723
Size: 818 6455
PmeI GTTT'AAAC
Cuts at: 4577 4577
Size: 7273
PmlI CAC'GTG
Cuts at: 2845 2845
Size: 7273
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3722 3754 5342 5374 6855 6887 3722
Size: 32 1588 32 1481 32 4108
Fragments arranged by size:
4108 1588 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3613 3902 3613
Size: 289 6984
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7273
PsiI TTA'TAA
Cuts at: 4843 4843
Size: 7273
PspXI vC'TCGA_Gb
Cuts at: 2070 2070
Size: 7273
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2318 2350 4511 4543 2318
Size: 32 2161 32 5048
Fragments arranged by size:
5048 2161 32 32
PstI C_TGCA'G
Cuts at: 830 2052 2106 2256 2879 3753 4625 830
Size: 1222 54 150 623 874 872 3478
Fragments arranged by size:
3478 1222 874 872 623 150 54
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7273
SacI G_AGCT'C
Cuts at: 2148 2148
Size: 7273
SacII CC_GC'GG
Cuts at: 2059 3865 2059
Size: 1806 5467
SalI G'TCGA_C
Cuts at: 1936 4615 1936
Size: 2679 4594
SapI GCTCTTCn'nnn_
Cuts at: 2889 2889
Size: 7273
SbfI CC_TGCA'GG
Cuts at: 4625 4625
Size: 7273
ScaI AGT'ACT
Cuts at: 4481 5521 7233 4481
Size: 1040 1712 4521
Fragments arranged by size:
4521 1712 1040
SfcI C'TryA_G
Cuts at: 826 1045 2048 2102 2252 2826 2875 3749 4003
Size: 219 1003 54 150 574 49 874 254
Cuts at: 4003 4153 4252 4621 5756 6437 6628 826
Size: 150 99 369 1135 681 191 1471
Fragments arranged by size:
1471 1135 1003 874 681 574 369 254
219 191 150 150 99 54 49
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7273
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7273
SmaI CCC'GGG
Cuts at: 1872 2176 2248 3189 4600 1872
Size: 304 72 941 1411 4545
Fragments arranged by size:
4545 1411 941 304 72
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2070 2078 2486 4216 5377 6278
Size: 197 926 127 8 408 1730 1161 901
Cuts at: 6278 6540 820
Size: 262 1553
Fragments arranged by size:
1730 1553 1161 926 901 408 262 197
127 8
SnaBI TAC'GTA
Cuts at: 493 5064 493
Size: 4571 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7273
SphI G_CATG'C
Cuts at: 1132 4631 1132
Size: 3499 3774
SrfI GCCC'GGGC
Cuts at: 2248 2248
Size: 7273
SspI AAT'ATT
Cuts at: 5 52 2314 5197 5
Size: 47 2262 2883 2081
Fragments arranged by size:
2883 2262 2081 47
StuI AGG'CCT
Cuts at: 1517 2076 2411 2524 1517
Size: 559 335 113 6266
Fragments arranged by size:
6266 559 335 113
StyI C'CwwG_G
Cuts at: 513 1065 2004 2304 3090 4712 513
Size: 552 939 300 786 1622 3074
Fragments arranged by size:
3074 1622 939 786 552 300
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2216 2467 2897 3473 3639 4162 5300
Size: 1255 636 251 430 576 166 523 1138
Cuts at: 5300 5317 5470 5655 6100 325
Size: 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1138 636 576 523 445 430
251 185 166 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3351 3783 4479
Size: 274 80 33 51 1443 1374 432 696
Cuts at: 4479 4565 5519 7231 96
Size: 86 954 1712 138
Fragments arranged by size:
1712 1443 1374 954 696 432 274 138
86 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3009 3041 7233 7265 3009
Size: 32 4192 32 3017
Fragments arranged by size:
4192 3017 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7273
XbaI T'CTAG_A
Cuts at: 4609 4609
Size: 7273
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3376 3376
Size: 7273
XhoI C'TCGA_G
Cuts at: 1943 2070 1943
Size: 127 7146
XmaI C'CCGG_G
Cuts at: 1870 2174 2246 3187 4598 1870
Size: 304 72 941 1411 4545
Fragments arranged by size:
4545 1411 941 304 72
XmnI GAAnn'nnTTC
Cuts at: 2330 5402 2330
Size: 3072 4201
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5081 276
Size: 53 83 186 936 3547 2468
Fragments arranged by size:
3547 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NruI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspXI PsrI PstI PvuI SacI
SacII SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BbeI BfrBI BsiWI BstXI
BstZ17I Bsu36I EcoRV FseI FspAI KasI NarI NsiI
PacI PspOMI PvuII RsrII SanDI SexAI SfiI SfoI
SwaI