Restriction map for
pFN21AB3093
(Circular) (Six-base) MAPSORT of: pFN21AB3093.seq Check: 5968 from: 1 to: 6940
pFN21AB3093 6940 bp
With 182 enzymes: SgfI *
February 27, 2009 14:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2108 2108
Size: 6940
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3219 4750 278
Size: 53 83 186 936 1683 1531 2468
Fragments arranged by size:
2468 1683 1531 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4283 1937
Size: 2346 4594
Acc65I G'GTAC_C
Cuts at: 4261 4261
Size: 6940
AclI AA'CG_TT
Cuts at: 1563 5067 5440 1563
Size: 3504 373 3063
Fragments arranged by size:
3504 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3203 5003 6387 6655 1632
Size: 1571 1800 1384 268 1917
Fragments arranged by size:
1917 1800 1571 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6743
AflIII A'CryG_T
Cuts at: 1203 5839 6816 1203
Size: 4636 977 1327
Fragments arranged by size:
4636 1327 977
AgeI A'CCGG_T
Cuts at: 5771 5771
Size: 6940
AhdI GACnn_n'nnGTC
Cuts at: 2997 5669 2997
Size: 2672 4268
AleI CACnn'nnGTG
Cuts at: 1166 2102 2318 1166
Size: 936 216 5788
Fragments arranged by size:
5788 936 216
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6940
AlwNI CAG_nnn'CTG
Cuts at: 2350 2887 3073 3781 4683 6255 2350
Size: 537 186 708 902 1572 3035
Fragments arranged by size:
3035 1572 902 708 537 186
ApaI G_GGCC'C
Cuts at: 2539 2940 2539
Size: 401 6539
ApaLI G'TGCA_C
Cuts at: 4997 6153 4997
Size: 1156 5784
ApoI r'AATT_y
Cuts at: 1493 4249 4475 4627 6736 1493
Size: 2756 226 152 2109 1697
Fragments arranged by size:
2756 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5494 160
Size: 872 4462 1606
Fragments arranged by size:
4462 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6940
AvaI C'yCGr_G
Cuts at: 1870 1943 2074 2086 2438 3717 4265 1870
Size: 73 131 12 352 1279 548 4545
Fragments arranged by size:
4545 1279 548 352 131 73 12
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2062 2095 2062
Size: 33 6907
BamHI G'GATC_C
Cuts at: 4270 4637 4270
Size: 367 6573
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2090 2154 2197 2780 2824
Size: 325 217 627 303 64 43 583 44
Cuts at: 2824 4261 5716 618
Size: 1437 1455 1842
Fragments arranged by size:
1842 1455 1437 627 583 325 303 217
64 44 43
BanII G_rGCy'C
Cuts at: 1391 2445 2539 2549 2940 4008 4080 4259 1391
Size: 1054 94 10 391 1068 72 179 4072
Fragments arranged by size:
4072 1068 1054 391 179 94 72 10
BbeI G_GCGC'C
Cuts at: 2094 2158 2784 2094
Size: 64 626 6250
Fragments arranged by size:
6250 626 64
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5969
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3908 3942 5131 5165 1903
Size: 34 1971 34 1189 34 3678
Fragments arranged by size:
3678 1971 1189 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2090 4832 6042 2090
Size: 2742 1210 2988
Fragments arranged by size:
2988 2742 1210
BclI T'GATC_A
Cuts at: 1548 2916 3516 1548
Size: 1368 600 4972
Fragments arranged by size:
4972 1368 600
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2420 2789 3416
Size: 107 122 71 631 605 748 369 627
Cuts at: 3416 4046 5551 136
Size: 630 1505 1525
Fragments arranged by size:
1525 1505 748 631 630 627 605 369
122 107 71
BglII A'GATC_T
Cuts at: 3741 6935 3741
Size: 3194 3746
BlpI GC'TnA_GC
Cuts at: 2708 4357 2708
Size: 1649 5291
Bme1580I G_kGCm'C
Cuts at: 1790 2109 2202 2276 2539 2829 2940 3245 3539
Size: 319 93 74 263 290 111 305 294
Cuts at: 3539 3926 4134 5001 6157 1790
Size: 387 208 867 1156 2573
Fragments arranged by size:
2573 1156 867 387 319 305 294 290
263 208 111 93 74
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6940
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4671 5629 442
Size: 416 53 83 399 24 3254 958 1753
Fragments arranged by size:
3254 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6940
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2314 3898 2314
Size: 1584 5356
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2240 3191 4105 4166 5600 1239
Size: 1001 951 914 61 1434 2579
Fragments arranged by size:
2579 1434 1001 951 914 61
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2153 2357 2993 5065 5930 6228 2153
Size: 204 636 2072 865 298 2865
Fragments arranged by size:
2865 2072 865 636 298 204
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5603 882
Size: 617 4104 2219
Fragments arranged by size:
4104 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3628 4731 493
Size: 711 2424 1103 2702
Fragments arranged by size:
2702 2424 1103 711
BsaBI GATnn'nnATC
Cuts at: 4067 6934 4067
Size: 2867 4073
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2091 2155 2525 2781
Size: 53 83 186 936 558 64 370 256
Cuts at: 2781 3216 3861 4747 5129 5764 275
Size: 435 645 886 382 635 1451
Fragments arranged by size:
1451 936 886 645 635 558 435 382
370 256 186 83 64 53
BsaWI w'CCGG_w
Cuts at: 3695 3984 5373 5771 6045 6192 6603 3695
Size: 289 1389 398 274 147 411 4032
Fragments arranged by size:
4032 1389 411 398 289 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2255 2285 2624 2654 1178
Size: 30 165 30 852 30 339 30 5464
Fragments arranged by size:
5464 852 339 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2150 3689 1186
Size: 964 1539 4437
Fragments arranged by size:
4437 1539 964
BseYI C'CCAG_C
Cuts at: 2204 2364 2553 3029 3451 3538 3618 6143 6639
Size: 160 189 476 422 87 80 2525 496
Cuts at: 6639 2204
Size: 2505
Fragments arranged by size:
2525 2505 496 476 422 189 160 87
80
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2599 2615 2738 2972 3131 3676 3689
Size: 573 863 16 123 234 159 545 13
Cuts at: 3689 3794 3890 1163
Size: 105 96 4213
Fragments arranged by size:
4213 863 573 545 234 159 123 105
96 16 13
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3858 4390 5151 6179 1540
Size: 275 186 1857 532 761 1028 2301
Fragments arranged by size:
2301 1857 1028 761 532 275 186
BsiHKAI G_wGCw'C
Cuts at: 2445 3009 3703 4008 5001 5086 6157 2445
Size: 564 694 305 993 85 1071 3228
Fragments arranged by size:
3228 1071 993 694 564 305 85
BsmI GAATG_Cn'
Cuts at: 4451 4544 4451
Size: 93 6847
BsmBI CGTCTCn'nnnn_
Cuts at: 3440 3440
Size: 6940
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2109 2202 2276 2445 2539 2549 2829
Size: 399 319 93 74 169 94 10 280
Cuts at: 2829 2940 3009 3245 3539 3703 3926 4008 4080
Size: 111 69 236 294 164 223 82 72
Cuts at: 4080 4134 4259 5001 5086 6157 1391
Size: 54 125 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 319 294 280 236
223 169 164 125 111 94 93 85
82 74 72 69 54 10
BspEI T'CCGG_A
Cuts at: 6603 6603
Size: 6940
BspHI T'CATG_A
Cuts at: 4829 6559 4829
Size: 1730 5210
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2108 2349 2769 4295 844
Size: 1264 241 420 1526 3489
Fragments arranged by size:
3489 1526 1264 420 241
BsrBI CCG'CTC
Cuts at: 1394 1823 4827 1394
Size: 429 3004 3507
Fragments arranged by size:
3507 3004 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5435 5609 65
Size: 1183 4187 174 1396
Fragments arranged by size:
4187 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5584 5771 1700
Size: 110 3774 187 2869
Fragments arranged by size:
3774 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6940
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6940
BssSI C'ACGA_G
Cuts at: 2948 5000 6012 2948
Size: 2052 1012 3876
Fragments arranged by size:
3876 2052 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2761 2959 794
Size: 1967 198 4775
Fragments arranged by size:
4775 1967 198
BstBI TT'CG_AA
Cuts at: 5744 5744
Size: 6940
BstEII G'GTnAC_C
Cuts at: 1184 2479 1184
Size: 1295 5645
BstXI CCAn_nnnn'nTGG
Cuts at: 2320 2599 2857 2320
Size: 279 258 6403
Fragments arranged by size:
6403 279 258
BstYI r'GATC_y
Cuts at: 1972 3741 4270 4637 5022 5039 6491 6577 6589
Size: 1769 529 367 385 17 1452 86 12
Cuts at: 6589 6935 1972
Size: 346 1977
Fragments arranged by size:
1977 1769 1452 529 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 3405 4202 3405
Size: 797 6143
BtgI C'CryG_G
Cuts at: 513 1065 2004 3237 3999 513
Size: 552 939 1233 762 3454
Fragments arranged by size:
3454 1233 939 762 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4140 487
Size: 683 56 2914 3287
Fragments arranged by size:
3287 2914 683 56
BtsI GCAGTG_nn'
Cuts at: 3701 3924 4218 4456 5250 5270 3701
Size: 223 294 238 794 20 5371
Fragments arranged by size:
5371 794 294 238 223 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6940
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2662 2697 529
Size: 35 2098 35 4772
Fragments arranged by size:
4772 2098 35 35
DraI TTT'AAA
Cuts at: 4244 4244
Size: 6940
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3221 5947 809
Size: 507 1905 2726 1802
Fragments arranged by size:
2726 1905 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2310 2352 2379
Size: 54 1425 50 275 18 480 42 27
Cuts at: 2379 2421 2490 2562 2790 2802 2859 2925 3150
Size: 42 69 72 228 12 57 66 225
Cuts at: 3150 3488 3855 4236 4387 5276 6905 8
Size: 338 367 381 151 889 1629 43
Fragments arranged by size:
1629 1425 889 480 381 367 338 275
228 225 151 72 69 66 57 54
50 43 42 42 27 18 12
EagI C'GGCC_G
Cuts at: 1537 1812 3855 4387 1537
Size: 275 2043 532 4090
Fragments arranged by size:
4090 2043 532 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4025 4870 1353
Size: 2672 845 3423
Fragments arranged by size:
3423 2672 845
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5527 5901 6047 387
Size: 1064 138 3938 374 146 1280
Fragments arranged by size:
3938 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2443 4006 2443
Size: 1563 5377
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2240 3191 3203 4105 4166 5003 5600
Size: 393 608 951 12 902 61 837 597
Cuts at: 5600 6387 6655 1239
Size: 787 268 1524
Fragments arranged by size:
1524 951 902 837 787 608 597 393
268 61 12
EcoNI CCTnn'n_nnAGG
Cuts at: 2771 2771
Size: 6940
EcoO109I rG'GnC_Cy
Cuts at: 2467 2536 2937 2944 3070 3166 3721 3879 4206
Size: 69 401 7 126 96 555 158 327
Cuts at: 4206 2467
Size: 5201
Fragments arranged by size:
5201 555 401 327 158 126 96 69
7
EcoRI G'AATT_C
Cuts at: 4249 4627 4249
Size: 378 6562
FspI TGC'GCA
Cuts at: 3134 5446 3134
Size: 2312 4628
HaeII r_GCGC'y
Cuts at: 1678 2094 2158 2784 3625 6087 1678
Size: 416 64 626 841 2462 2531
Fragments arranged by size:
2531 2462 841 626 416 64
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2983 3015 3508 3540 5581
Size: 32 135 32 1411 32 493 32 2041
Cuts at: 5581 5613 5655 5687 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2041 1411 493 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3220 4284 4530 669
Size: 988 281 1282 1064 246 3079
Fragments arranged by size:
3079 1282 1064 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6940
HpaI GTT'AAC
Cuts at: 4530 4530
Size: 6940
KasI G'GCGC_C
Cuts at: 2090 2154 2780 2090
Size: 64 626 6250
Fragments arranged by size:
6250 626 64
KpnI G_GTAC'C
Cuts at: 4265 4265
Size: 6940
MfeI C'AATT_G
Cuts at: 4539 4539
Size: 6940
MluI A'CGCG_T
Cuts at: 6816 6816
Size: 6940
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3276 3613 6054 6238 1305
Size: 1971 337 2441 184 2007
Fragments arranged by size:
2441 2007 1971 337 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2312 2423 2861 2927 3152
Size: 54 1425 343 480 111 438 66 225
Cuts at: 3152 3490 10
Size: 338 3460
Fragments arranged by size:
3460 1425 480 438 343 338 225 111
66 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2102 2318 2498 3612 4160 4898
Size: 648 843 93 216 180 1114 548 738
Cuts at: 4898 5257 5416 518
Size: 359 159 2042
Fragments arranged by size:
2042 1114 843 738 648 548 359 216
180 159 93
MspA1I CmG'CkG
Cuts at: 1606 2368 2509 2557 2567 2613 3202 3303 3556
Size: 762 141 48 10 46 589 101 253
Cuts at: 3556 3569 4144 4356 5033 6181 6426 1606
Size: 13 575 212 677 1148 245 2120
Fragments arranged by size:
2120 1148 762 677 589 575 253 245
212 141 101 48 46 13 10
NarI GG'CG_CC
Cuts at: 2091 2155 2781 2091
Size: 64 626 6250
Fragments arranged by size:
6250 626 64
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5449
Fragments arranged by size:
5449 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6940
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6940
NotI GC'GGCC_GC
Cuts at: 4387 4387
Size: 6940
NspI r_CATG'y
Cuts at: 1132 1229 2707 3264 4234 4298 5843 1132
Size: 97 1478 557 970 64 1545 2229
Fragments arranged by size:
2229 1545 1478 970 557 97 64
PasI CC'CwG_GG
Cuts at: 1348 2191 3073 1348
Size: 843 882 5215
Fragments arranged by size:
5215 882 843
PciI A'CATG_T
Cuts at: 5839 5839
Size: 6940
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2560 3486 3595 3811 4234 1108
Size: 1452 926 109 216 423 3814
Fragments arranged by size:
3814 1452 926 423 216 109
PmeI GTTT'AAAC
Cuts at: 4244 4244
Size: 6940
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5009 5041 6522 6554 5009
Size: 32 1481 32 5395
Fragments arranged by size:
5395 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2467 3721 2467
Size: 1254 5686
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6940
PsiI TTA'TAA
Cuts at: 4510 4510
Size: 6940
PspOMI G'GGCC_C
Cuts at: 2535 2936 2535
Size: 401 6539
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3965 3997 3965
Size: 32 6908
PstI C_TGCA'G
Cuts at: 830 2346 2883 3561 3637 4225 4292 830
Size: 1516 537 678 76 588 67 3478
Fragments arranged by size:
3478 1516 678 588 537 76 67
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6940
PvuII CAG'CTG
Cuts at: 2368 2509 2557 3202 2368
Size: 141 48 645 6106
Fragments arranged by size:
6106 645 141 48
SacI G_AGCT'C
Cuts at: 2445 4008 2445
Size: 1563 5377
SalI G'TCGA_C
Cuts at: 1936 4282 1936
Size: 2346 4594
SanDI GG'GwC_CC
Cuts at: 3721 3721
Size: 6940
SbfI CC_TGCA'GG
Cuts at: 4292 4292
Size: 6940
ScaI AGT'ACT
Cuts at: 5188 6900 5188
Size: 1712 5228
SfcI C'TryA_G
Cuts at: 826 1045 2342 2879 2968 3557 3633 4189 4221
Size: 219 1297 537 89 589 76 556 32
Cuts at: 4221 4288 5423 6104 6295 826
Size: 67 1135 681 191 1471
Fragments arranged by size:
1471 1297 1135 681 589 556 537 219
191 89 76 67 32
SfiI GGCCn_nnn'nGGCC
Cuts at: 3416 3416
Size: 6940
SfoI GGC'GCC
Cuts at: 2092 2156 2782 2092
Size: 64 626 6250
Fragments arranged by size:
6250 626 64
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6940
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6940
SmaI CCC'GGG
Cuts at: 1872 3719 4267 1872
Size: 1847 548 4545
Fragments arranged by size:
4545 1847 548
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2168 2372 3008 5044 5945 6207
Size: 197 926 225 204 636 2036 901 262
Cuts at: 6207 820
Size: 1553
Fragments arranged by size:
2036 1553 926 901 636 262 225 204
197
SnaBI TAC'GTA
Cuts at: 493 4731 493
Size: 4238 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6940
SphI G_CATG'C
Cuts at: 1132 2707 4298 1132
Size: 1575 1591 3774
Fragments arranged by size:
3774 1591 1575
SspI AAT'ATT
Cuts at: 5 52 4864 5
Size: 47 4812 2081
Fragments arranged by size:
4812 2081 47
StuI AGG'CCT
Cuts at: 1517 2147 1517
Size: 630 6310
StyI C'CwwG_G
Cuts at: 513 1065 2004 2209 2484 4379 513
Size: 552 939 205 275 1895 3074
Fragments arranged by size:
3074 1895 939 552 275 205
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2204 2330 2716 4967 4984 5137 5322
Size: 1255 624 126 386 2251 17 153 185
Cuts at: 5322 5767 325
Size: 445 1498
Fragments arranged by size:
2251 1498 1255 624 445 386 185 153
126 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2160 3783 4116
Size: 274 80 33 51 1443 183 1623 333
Cuts at: 4116 5186 6898 96
Size: 1070 1712 138
Fragments arranged by size:
1712 1623 1443 1070 333 274 183 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2255 2287 2624 2656 3693 3725 6900 6932 2255
Size: 32 337 32 1037 32 3175 32 2263
Fragments arranged by size:
3175 2263 1037 337 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6940
XbaI T'CTAG_A
Cuts at: 4276 4276
Size: 6940
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3733 3733
Size: 6940
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6940
XmaI C'CCGG_G
Cuts at: 1870 3717 4265 1870
Size: 1847 548 4545
Fragments arranged by size:
4545 1847 548
XmnI GAAnn'nnTTC
Cuts at: 5069 5069
Size: 6940
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3217 4748 276
Size: 53 83 186 936 1683 1531 2468
Fragments arranged by size:
2468 1683 1531 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NarI NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI
PsrI PstI PvuI PvuII SacI SalI SanDI SbfI
ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BbvCI BfrBI Bpu10I BsiWI
BstZ17I DraIII EcoRV FalI FseI FspAI NaeI NgoMIV
NruI NsiI PacI PfoI PmlI PspXI RsrII SacII
SapI SexAI SrfI SwaI