Restriction map for
pFN21AB3092
(Circular) (Six-base) MAPSORT of: pFN21AB3092.seq Check: 3277 from: 1 to: 6853
pFN21AB3092 6853 bp
With 182 enzymes: SgfI *
February 27, 2009 14:34 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2485 4663 278
Size: 53 83 186 936 949 2178 2468
Fragments arranged by size:
2468 2178 949 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2492 4196 1937
Size: 555 1704 4594
Fragments arranged by size:
4594 1704 555
Acc65I G'GTAC_C
Cuts at: 4174 4174
Size: 6853
AclI AA'CG_TT
Cuts at: 1563 4980 5353 1563
Size: 3417 373 3063
Fragments arranged by size:
3417 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2405 2637 4916 6300 6568 1632
Size: 773 232 2279 1384 268 1917
Fragments arranged by size:
2279 1917 1384 773 268 232
AflII C'TTAA_G
Cuts at: 820 1017 2185 820
Size: 197 1168 5488
Fragments arranged by size:
5488 1168 197
AflIII A'CryG_T
Cuts at: 1203 2851 3721 5752 6729 1203
Size: 1648 870 2031 977 1327
Fragments arranged by size:
2031 1648 1327 977 870
AgeI A'CCGG_T
Cuts at: 5684 5684
Size: 6853
AhdI GACnn_n'nnGTC
Cuts at: 2481 5582 2481
Size: 3101 3752
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6853
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6853
AlwNI CAG_nnn'CTG
Cuts at: 2981 4596 6168 2981
Size: 1615 1572 3666
Fragments arranged by size:
3666 1615 1572
ApaLI G'TGCA_C
Cuts at: 4910 6066 4910
Size: 1156 5697
ApoI r'AATT_y
Cuts at: 1493 2900 2921 2955 3053 3375 3561 3905 4162
Size: 1407 21 34 98 322 186 344 257
Cuts at: 4162 4388 4540 6649 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 1407 344 322 257 226 186
152 98 34 21
AseI AT'TA_AT
Cuts at: 160 1032 5407 160
Size: 872 4375 1606
Fragments arranged by size:
4375 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6853
AvaI C'yCGr_G
Cuts at: 1870 1943 2448 3000 3438 4178 1870
Size: 73 505 552 438 740 4545
Fragments arranged by size:
4545 740 552 505 438 73
BamHI G'GATC_C
Cuts at: 3047 4183 4550 3047
Size: 1136 367 5350
Fragments arranged by size:
5350 1136 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4174 5629 618
Size: 325 217 627 2387 1455 1842
Fragments arranged by size:
2387 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2398 3769 4172 1391
Size: 1007 1371 403 4072
Fragments arranged by size:
4072 1371 1007 403
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5882
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2008 2042 5044 5078 1903
Size: 34 71 34 3002 34 3678
Fragments arranged by size:
3678 3002 71 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4745 5955 4745
Size: 1210 5643
BclI T'GATC_A
Cuts at: 1548 2227 3409 1548
Size: 679 1182 4992
Fragments arranged by size:
4992 1182 679
BfrBI ATG'CAT
Cuts at: 2633 3190 2633
Size: 557 6296
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3706 5464 136
Size: 107 122 71 631 605 2034 1758 1525
Fragments arranged by size:
2034 1758 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2416 6848 2416
Size: 4432 2421
BlpI GC'TnA_GC
Cuts at: 4270 4270
Size: 6853
Bme1580I G_kGCm'C
Cuts at: 1790 4914 6070 1790
Size: 3124 1156 2573
Fragments arranged by size:
3124 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6853
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4584 5542 442
Size: 416 53 83 399 24 3167 958 1753
Fragments arranged by size:
3167 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6853
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2471 3563 5513 1239
Size: 1232 1092 1950 2579
Fragments arranged by size:
2579 1950 1232 1092
Bpu10I CC'TnA_GC
Cuts at: 2148 2148
Size: 6853
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2655 4148 4978 5843 6141 2655
Size: 1493 830 865 298 3367
Fragments arranged by size:
3367 1493 865 830 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5516 882
Size: 617 4017 2219
Fragments arranged by size:
4017 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4644 493
Size: 711 3440 2702
Fragments arranged by size:
3440 2702 711
BsaBI GATnn'nnATC
Cuts at: 6847 6847
Size: 6853
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2482 4660 5042 5677
Size: 53 83 186 936 949 2178 382 635
Cuts at: 5677 275
Size: 1451
Fragments arranged by size:
2178 1451 949 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2787 3458 5286 5684 5958 6105 6516 2787
Size: 671 1828 398 274 147 411 3124
Fragments arranged by size:
3124 1828 671 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2100 2130 2469 2499 3530
Size: 30 165 30 697 30 339 30 1031
Cuts at: 3530 3560 3792 3822 1178
Size: 30 232 30 4209
Fragments arranged by size:
4209 1031 697 339 232 165 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3830 1186
Size: 2644 4209
BseYI C'CCAG_C
Cuts at: 2162 3768 6056 6552 2162
Size: 1606 2288 496 2463
Fragments arranged by size:
2463 2288 1606 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2292 1163
Size: 573 556 5724
Fragments arranged by size:
5724 573 556
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4303 5064 6092 1540
Size: 275 186 2302 761 1028 2301
Fragments arranged by size:
2302 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2024 2398 4914 4999 6070 2024
Size: 374 2516 85 1071 2807
Fragments arranged by size:
2807 2516 1071 374 85
BsmI GAATG_Cn'
Cuts at: 3127 4364 4457 3127
Size: 1237 93 5523
Fragments arranged by size:
5523 1237 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2489 2489
Size: 6853
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2024 2398 3769 4172 4914 4999 6070
Size: 399 234 374 1371 403 742 85 1071
Cuts at: 6070 1391
Size: 2174
Fragments arranged by size:
2174 1371 1071 742 403 399 374 234
85
BspEI T'CCGG_A
Cuts at: 2787 3458 6516 2787
Size: 671 3058 3124
Fragments arranged by size:
3124 3058 671
BspHI T'CATG_A
Cuts at: 2595 3628 4742 6472 2595
Size: 1033 1114 1730 2976
Fragments arranged by size:
2976 1730 1114 1033
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4208 844
Size: 3364 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4740 1394
Size: 429 2917 3507
Fragments arranged by size:
3507 2917 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3678 5348 5522 65
Size: 1183 2430 1670 174 1396
Fragments arranged by size:
2430 1670 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5497 5684 1700
Size: 110 3687 187 2869
Fragments arranged by size:
3687 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6853
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6853
BssSI C'ACGA_G
Cuts at: 4913 5925 4913
Size: 1012 5841
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6853
BstBI TT'CG_AA
Cuts at: 5657 5657
Size: 6853
BstEII G'GTnAC_C
Cuts at: 1184 3526 3898 1184
Size: 2342 372 4139
Fragments arranged by size:
4139 2342 372
BstYI r'GATC_y
Cuts at: 1972 2416 2818 3047 4183 4550 4935 4952 6404
Size: 444 402 229 1136 367 385 17 1452
Cuts at: 6404 6490 6502 6848 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1136 444 402 385 367 346
229 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2702 2702
Size: 6853
BtgI C'CryG_G
Cuts at: 513 1065 3701 513
Size: 552 2636 3665
Fragments arranged by size:
3665 2636 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6114
Fragments arranged by size:
6114 683 56
BtsI GCAGTG_nn'
Cuts at: 2173 4369 5163 5183 2173
Size: 2196 794 20 3843
Fragments arranged by size:
3843 2196 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6853
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6818
DraI TTT'AAA
Cuts at: 2458 2638 3244 4157 2458
Size: 180 606 913 5154
Fragments arranged by size:
5154 913 606 180
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5860 809
Size: 507 4544 1802
Fragments arranged by size:
4544 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2429 4300 5189
Size: 54 1425 50 275 18 599 1871 889
Cuts at: 5189 6818 8
Size: 1629 43
Fragments arranged by size:
1871 1629 1425 889 599 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4300 1537
Size: 275 2488 4090
Fragments arranged by size:
4090 2488 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2403 2729 3164 3799 4783 1353
Size: 1050 326 435 635 984 3423
Fragments arranged by size:
3423 1050 984 635 435 326
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5440 5814 5960 387
Size: 1064 138 3851 374 146 1280
Fragments arranged by size:
3851 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2396 2396
Size: 6853
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2405 2471 2637 3563 4916 5513 6300
Size: 393 773 66 166 926 1353 597 787
Cuts at: 6300 6568 1239
Size: 268 1524
Fragments arranged by size:
1524 1353 926 787 773 597 393 268
166 66
EcoO109I rG'GnC_Cy
Cuts at: 2191 3572 2191
Size: 1381 5472
EcoRI G'AATT_C
Cuts at: 2900 2955 4162 4540 2900
Size: 55 1207 378 5213
Fragments arranged by size:
5213 1207 378 55
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2975 2975
Size: 6853
FspI TGC'GCA
Cuts at: 5359 5359
Size: 6853
HaeII r_GCGC'y
Cuts at: 1678 2531 6000 1678
Size: 853 3469 2531
Fragments arranged by size:
3469 2531 853
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2467 2499 2552 2584 3199
Size: 32 135 32 895 32 53 32 615
Cuts at: 3199 3231 5494 5526 5568 5600 1373
Size: 32 2263 32 42 32 2626
Fragments arranged by size:
2626 2263 895 615 135 53 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4197 4443 669
Size: 988 281 2259 246 3079
Fragments arranged by size:
3079 2259 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3923 4077 748
Size: 3175 154 3524
Fragments arranged by size:
3524 3175 154
HpaI GTT'AAC
Cuts at: 4443 4443
Size: 6853
KpnI G_GTAC'C
Cuts at: 4178 4178
Size: 6853
MfeI C'AATT_G
Cuts at: 4452 4452
Size: 6853
MluI A'CGCG_T
Cuts at: 6729 6729
Size: 6853
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2947 3450 4122 5967 6151 1305
Size: 1642 503 672 1845 184 2007
Fragments arranged by size:
2007 1845 1642 672 503 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2431 10
Size: 54 1425 343 599 4432
Fragments arranged by size:
4432 1425 599 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2136 2628 4811 5170 5329 518
Size: 648 970 492 2183 359 159 2042
Fragments arranged by size:
2183 2042 970 648 492 359 159
MspA1I CmG'CkG
Cuts at: 1606 2233 3506 3772 4269 4946 6094 6339 1606
Size: 627 1273 266 497 677 1148 245 2120
Fragments arranged by size:
2120 1273 1148 677 627 497 266 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6301
NdeI CA'TA_TG
Cuts at: 387 4018 387
Size: 3631 3222
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6853
NotI GC'GGCC_GC
Cuts at: 4300 4300
Size: 6853
NsiI A_TGCA'T
Cuts at: 2635 3192 2635
Size: 557 6296
NspI r_CATG'y
Cuts at: 1132 1229 2855 3334 3725 4211 5756 1132
Size: 97 1626 479 391 486 1545 2229
Fragments arranged by size:
2229 1626 1545 486 479 391 97
PasI CC'CwG_GG
Cuts at: 1348 2975 1348
Size: 1627 5226
PciI A'CATG_T
Cuts at: 2851 3721 5752 2851
Size: 870 2031 3952
Fragments arranged by size:
3952 2031 870
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2981 3716 1108
Size: 1873 735 4245
Fragments arranged by size:
4245 1873 735
PfoI T'CCnGG_A
Cuts at: 2251 2251
Size: 6853
PmeI GTTT'AAAC
Cuts at: 4157 4157
Size: 6853
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2127 2159 4922 4954 6435 6467 2127
Size: 32 2763 32 1481 32 2513
Fragments arranged by size:
2763 2513 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2191 2191
Size: 6853
PshAI GACnn'nnGTC
Cuts at: 1613 3724 1613
Size: 2111 4742
PsiI TTA'TAA
Cuts at: 4423 4423
Size: 6853
PspXI vC'TCGA_Gb
Cuts at: 3438 3438
Size: 6853
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2347 2379 2347
Size: 32 6821
PstI C_TGCA'G
Cuts at: 830 2815 4205 830
Size: 1985 1390 3478
Fragments arranged by size:
3478 1985 1390
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6853
PvuII CAG'CTG
Cuts at: 2233 3506 3772 2233
Size: 1273 266 5314
Fragments arranged by size:
5314 1273 266
SacI G_AGCT'C
Cuts at: 2398 2398
Size: 6853
SalI G'TCGA_C
Cuts at: 1936 4195 1936
Size: 2259 4594
SapI GCTCTTCn'nnn_
Cuts at: 2403 2403
Size: 6853
SbfI CC_TGCA'GG
Cuts at: 4205 4205
Size: 6853
ScaI AGT'ACT
Cuts at: 2426 5101 6813 2426
Size: 2675 1712 2466
Fragments arranged by size:
2675 2466 1712
SfcI C'TryA_G
Cuts at: 826 1045 2811 3940 4201 5336 6017 6208 826
Size: 219 1766 1129 261 1135 681 191 1471
Fragments arranged by size:
1766 1471 1135 1129 681 261 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6853
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6853
SmaI CCC'GGG
Cuts at: 1872 4180 1872
Size: 2308 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2185 2670 3438 4127 4957 5858
Size: 197 926 242 485 768 689 830 901
Cuts at: 5858 6120 820
Size: 262 1553
Fragments arranged by size:
1553 926 901 830 768 689 485 262
242 197
SnaBI TAC'GTA
Cuts at: 493 4644 493
Size: 4151 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6853
SphI G_CATG'C
Cuts at: 1132 4211 1132
Size: 3079 3774
SspI AAT'ATT
Cuts at: 5 52 4777 5
Size: 47 4725 2081
Fragments arranged by size:
4725 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6853
StyI C'CwwG_G
Cuts at: 513 1065 2194 3347 4292 513
Size: 552 1129 1153 945 3074
Fragments arranged by size:
3074 1153 1129 945 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3062 4880 4897 5050 5235 5680 325
Size: 1255 1482 1818 17 153 185 445 1498
Fragments arranged by size:
1818 1498 1482 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2354 2424 5099
Size: 274 80 33 51 1443 377 70 2675
Cuts at: 5099 6811 96
Size: 1712 138
Fragments arranged by size:
2675 1712 1443 377 274 138 80 70
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2469 2501 3792 3824 6813 6845 2469
Size: 32 1291 32 2989 32 2477
Fragments arranged by size:
2989 2477 1291 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6853
XbaI T'CTAG_A
Cuts at: 4189 4189
Size: 6853
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3538 3538
Size: 6853
XhoI C'TCGA_G
Cuts at: 1943 3438 1943
Size: 1495 5358
XmaI C'CCGG_G
Cuts at: 1870 4178 1870
Size: 2308 4545
XmnI GAAnn'nnTTC
Cuts at: 2658 4982 2658
Size: 2324 4529
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2483 4661 276
Size: 53 83 186 936 949 2178 2468
Fragments arranged by size:
2468 2178 949 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspXI PsrI PstI PvuI PvuII
SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI BsiWI BstXI BstZ17I DraIII EcoNI EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PmlI PspOMI RsrII SacII SanDI SexAI SfiI SfoI
SrfI SwaI