Restriction map for
pFN21AB3091
(Circular) (Six-base) MAPSORT of: pFN21AB3091.seq Check: 6127 from: 1 to: 7507
pFN21AB3091 7507 bp
With 182 enzymes: SgfI *
February 27, 2009 14:45 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5317 278
Size: 53 83 186 936 3781 2468
Fragments arranged by size:
3781 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3692 3969 4850 1937
Size: 1755 277 881 4594
Fragments arranged by size:
4594 1755 881 277
Acc65I G'GTAC_C
Cuts at: 4828 4828
Size: 7507
AclI AA'CG_TT
Cuts at: 1563 5634 6007 1563
Size: 4071 373 3063
Fragments arranged by size:
4071 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2875 3744 4446 4764 5570 6954 7222 1632
Size: 1243 869 702 318 806 1384 268 1917
Fragments arranged by size:
1917 1384 1243 869 806 702 318 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7310
AflIII A'CryG_T
Cuts at: 1203 3834 4405 6406 7383 1203
Size: 2631 571 2001 977 1327
Fragments arranged by size:
2631 2001 1327 977 571
AgeI A'CCGG_T
Cuts at: 2730 6338 2730
Size: 3608 3899
AhdI GACnn_n'nnGTC
Cuts at: 6236 6236
Size: 7507
AleI CACnn'nnGTG
Cuts at: 1166 2102 1166
Size: 936 6571
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4636 1184
Size: 3452 4055
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3837 3869 3837
Size: 32 7475
AlwNI CAG_nnn'CTG
Cuts at: 2980 3265 5250 6822 2980
Size: 285 1985 1572 3665
Fragments arranged by size:
3665 1985 1572 285
ApaLI G'TGCA_C
Cuts at: 3552 5564 6720 3552
Size: 2012 1156 4339
Fragments arranged by size:
4339 2012 1156
ApoI r'AATT_y
Cuts at: 1493 2254 2534 2667 3085 3404 3653 4816 5042
Size: 761 280 133 418 319 249 1163 226
Cuts at: 5042 5194 7303 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 1163 761 418 319 280 249
226 152 133
AseI AT'TA_AT
Cuts at: 160 1032 3491 4235 6061 160
Size: 872 2459 744 1826 1606
Fragments arranged by size:
2459 1826 1606 872 744
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7507
AvaI C'yCGr_G
Cuts at: 1870 1943 2166 4134 4832 1870
Size: 73 223 1968 698 4545
Fragments arranged by size:
4545 1968 698 223 73
BamHI G'GATC_C
Cuts at: 4837 5204 4837
Size: 367 7140
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4828 6283 618
Size: 325 217 627 3041 1455 1842
Fragments arranged by size:
3041 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2932 4826 1391
Size: 1541 1894 4072
Fragments arranged by size:
4072 1894 1541
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2406 928
Size: 971 507 6029
Fragments arranged by size:
6029 971 507
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5698 5732 1903
Size: 34 3761 34 3678
Fragments arranged by size:
3761 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5399 6609 5399
Size: 1210 6297
BclI T'GATC_A
Cuts at: 1548 2193 2548 2673 4471 1548
Size: 645 355 125 1798 4584
Fragments arranged by size:
4584 1798 645 355 125
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6118 136
Size: 107 122 71 631 605 4446 1525
Fragments arranged by size:
4446 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2275 3160 3508 7502 2275
Size: 885 348 3994 2280
Fragments arranged by size:
3994 2280 885 348
BlpI GC'TnA_GC
Cuts at: 3804 4924 3804
Size: 1120 6387
Bme1580I G_kGCm'C
Cuts at: 1790 3556 5568 6724 1790
Size: 1766 2012 1156 2573
Fragments arranged by size:
2573 2012 1766 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7507
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4059 5238 6196
Size: 416 53 83 399 24 2642 1179 958
Cuts at: 6196 442
Size: 1753
Fragments arranged by size:
2642 1753 1179 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7507
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2646 6167 1239
Size: 1407 3521 2579
Fragments arranged by size:
3521 2579 1407
Bpu10I CC'TnA_GC
Cuts at: 4515 4515
Size: 7507
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2585 2997 4577 5632 6497 6795 2585
Size: 412 1580 1055 865 298 3297
Fragments arranged by size:
3297 1580 1055 865 412 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4424 6170 882
Size: 617 2925 1746 2219
Fragments arranged by size:
2925 2219 1746 617
BsaAI yAC'GTr
Cuts at: 493 1204 3835 5298 493
Size: 711 2631 1463 2702
Fragments arranged by size:
2702 2631 1463 711
BsaBI GATnn'nnATC
Cuts at: 2547 3384 3432 7501 2547
Size: 837 48 4069 2553
Fragments arranged by size:
4069 2553 837 48
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5314 5696 6331 275
Size: 53 83 186 936 3781 382 635 1451
Fragments arranged by size:
3781 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2730 5940 6338 6612 6759 7170 2730
Size: 3210 398 274 147 411 3067
Fragments arranged by size:
3210 3067 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3837 3867 4553 4583 4652
Size: 30 165 30 2434 30 686 30 69
Cuts at: 4652 4682 1178
Size: 30 4003
Fragments arranged by size:
4003 2434 686 165 69 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2564 2628 2676 3089 3309 4574 1186
Size: 1378 64 48 413 220 1265 4119
Fragments arranged by size:
4119 1378 1265 413 220 64 48
BseYI C'CCAG_C
Cuts at: 6710 7206 6710
Size: 496 7011
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2033 3519 1163
Size: 573 297 1486 5151
Fragments arranged by size:
5151 1486 573 297
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2049 2119 4957 5718 6746 1540
Size: 275 186 48 70 2838 761 1028 2301
Fragments arranged by size:
2838 2301 1028 761 275 186 70 48
BsiHKAI G_wGCw'C
Cuts at: 3556 3850 5568 5653 6724 3556
Size: 294 1718 85 1071 4339
Fragments arranged by size:
4339 1718 1071 294 85
BsmI GAATG_Cn'
Cuts at: 5018 5111 5018
Size: 93 7414
BsmBI CGTCTCn'nnnn_
Cuts at: 2018 2018
Size: 7507
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2932 3556 3850 4826 5568 5653 6724
Size: 399 1142 624 294 976 742 85 1071
Cuts at: 6724 1391
Size: 2174
Fragments arranged by size:
2174 1142 1071 976 742 624 399 294
85
BspEI T'CCGG_A
Cuts at: 7170 7170
Size: 7507
BspHI T'CATG_A
Cuts at: 4357 5396 7126 4357
Size: 1039 1730 4738
Fragments arranged by size:
4738 1730 1039
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4862 844
Size: 4018 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 5394 1394
Size: 429 3571 3507
Fragments arranged by size:
3571 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4311 6002 6176 65
Size: 1183 3063 1691 174 1396
Fragments arranged by size:
3063 1691 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2209 2730 6151 6338 1700
Size: 110 399 521 3421 187 2869
Fragments arranged by size:
3421 2869 521 399 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7507
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7507
BssSI C'ACGA_G
Cuts at: 5567 6579 5567
Size: 1012 6495
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4502 794
Size: 3708 3799
BstBI TT'CG_AA
Cuts at: 6311 6311
Size: 7507
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7507
BstXI CCAn_nnnn'nTGG
Cuts at: 3644 4180 3644
Size: 536 6971
BstYI r'GATC_y
Cuts at: 1972 2275 3160 3508 4837 5204 5589 5606 7058
Size: 303 885 348 1329 367 385 17 1452
Cuts at: 7058 7144 7156 7502 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1329 885 385 367 348 346
303 86 17 12
BstZ17I GTA'TAC
Cuts at: 3693 3970 3693
Size: 277 7230
Bsu36I CC'TnA_GG
Cuts at: 4211 4211
Size: 7507
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 6016
Fragments arranged by size:
6016 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6768
Fragments arranged by size:
6768 683 56
BtsI GCAGTG_nn'
Cuts at: 5023 5817 5837 5023
Size: 794 20 6693
Fragments arranged by size:
6693 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7507
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7472
DraI TTT'AAA
Cuts at: 3681 4811 3681
Size: 1130 6377
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6514 809
Size: 507 5198 1802
Fragments arranged by size:
5198 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2046 2160 2473
Size: 54 1425 50 275 18 216 114 313
Cuts at: 2473 4374 4954 5843 7472 8
Size: 1901 580 889 1629 43
Fragments arranged by size:
1901 1629 1425 889 580 313 275 216
114 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2046 4954 1537
Size: 275 234 2908 4090
Fragments arranged by size:
4090 2908 275 234
EarI CTCTTCn'nnn_
Cuts at: 1353 3681 4252 4422 5437 1353
Size: 2328 571 170 1015 3423
Fragments arranged by size:
3423 2328 1015 571 170
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6094 6468 6614 387
Size: 1064 138 4505 374 146 1280
Fragments arranged by size:
4505 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2646 2875 3744 4446 4764 5570 6167
Size: 393 1014 229 869 702 318 806 597
Cuts at: 6167 6954 7222 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1014 869 806 787 702 597 393
318 268 229
EcoNI CCTnn'n_nnAGG
Cuts at: 2942 4670 2942
Size: 1728 5779
EcoO109I rG'GnC_Cy
Cuts at: 2722 2722
Size: 7507
EcoRI G'AATT_C
Cuts at: 2534 2667 3404 4816 5194 2534
Size: 133 737 1412 378 4847
Fragments arranged by size:
4847 1412 737 378 133
EcoRV GAT'ATC
Cuts at: 3634 4597 3634
Size: 963 6544
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2297 3161 4281 2297
Size: 864 1120 5523
Fragments arranged by size:
5523 1120 864
FspI TGC'GCA
Cuts at: 6013 6013
Size: 7507
HaeII r_GCGC'y
Cuts at: 1678 6654 1678
Size: 4976 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2540 2572 4581 4613 4652
Size: 32 135 32 968 32 2009 32 39
Cuts at: 4652 4684 6148 6180 6222 6254 1373
Size: 32 1464 32 42 32 2626
Fragments arranged by size:
2626 2009 1464 968 135 42 39 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3031 3486 4851 5097 669
Size: 988 281 1093 455 1365 246 3079
Fragments arranged by size:
3079 1365 1093 988 455 281 246
HindIII A'AGCT_T
Cuts at: 748 3096 748
Size: 2348 5159
HpaI GTT'AAC
Cuts at: 3031 5097 3031
Size: 2066 5441
KpnI G_GTAC'C
Cuts at: 4832 4832
Size: 7507
MfeI C'AATT_G
Cuts at: 4157 5106 4157
Size: 949 6558
MluI A'CGCG_T
Cuts at: 7383 7383
Size: 7507
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2016 2467 2880 4173 6621 6805 1305
Size: 711 451 413 1293 2448 184 2007
Fragments arranged by size:
2448 2007 1293 711 451 413 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2475 4376 10
Size: 54 1425 343 643 1901 3141
Fragments arranged by size:
3141 1901 1425 643 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2102 3924 4178 4356 5465 5824
Size: 648 843 93 1822 254 178 1109 359
Cuts at: 5824 5983 518
Size: 159 2042
Fragments arranged by size:
2042 1822 1109 843 648 359 254 178
159 93
MspA1I CmG'CkG
Cuts at: 1606 4923 5600 6748 6993 1606
Size: 3317 677 1148 245 2120
Fragments arranged by size:
3317 2120 1148 677 245
NaeI GCC'GGC
Cuts at: 2211 2211
Size: 7507
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 6016
Fragments arranged by size:
6016 939 552
NdeI CA'TA_TG
Cuts at: 387 3859 387
Size: 3472 4035
NgoMIV G'CCGG_C
Cuts at: 2209 2209
Size: 7507
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7507
NotI GC'GGCC_GC
Cuts at: 4954 4954
Size: 7507
NspI r_CATG'y
Cuts at: 1132 1229 2083 3923 4342 4409 4523 4865 6410
Size: 97 854 1840 419 67 114 342 1545
Cuts at: 6410 1132
Size: 2229
Fragments arranged by size:
2229 1840 1545 854 419 342 114 97
67
PasI CC'CwG_GG
Cuts at: 1348 2185 1348
Size: 837 6670
PciI A'CATG_T
Cuts at: 4405 6406 4405
Size: 2001 5506
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7507
PmeI GTTT'AAAC
Cuts at: 4811 4811
Size: 7507
PmlI CAC'GTG
Cuts at: 3835 3835
Size: 7507
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3837 3869 5576 5608 7089 7121 3837
Size: 32 1707 32 1481 32 4223
Fragments arranged by size:
4223 1707 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2722 2722
Size: 7507
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7507
PsiI TTA'TAA
Cuts at: 2313 5077 2313
Size: 2764 4743
PstI C_TGCA'G
Cuts at: 830 2034 2232 4859 830
Size: 1204 198 2627 3478
Fragments arranged by size:
3478 2627 1204 198
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7507
SalI G'TCGA_C
Cuts at: 1936 4849 1936
Size: 2913 4594
SbfI CC_TGCA'GG
Cuts at: 4859 4859
Size: 7507
ScaI AGT'ACT
Cuts at: 3625 4062 5755 7467 3625
Size: 437 1693 1712 3665
Fragments arranged by size:
3665 1712 1693 437
SfcI C'TryA_G
Cuts at: 826 1045 2030 2228 3627 4855 5990 6671 6862
Size: 219 985 198 1399 1228 1135 681 191
Cuts at: 6862 826
Size: 1471
Fragments arranged by size:
1471 1399 1228 1135 985 681 219 198
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7507
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7507
SmaI CCC'GGG
Cuts at: 1872 2168 4834 1872
Size: 296 2666 4545
Fragments arranged by size:
4545 2666 296
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2564 3012 4556 5611 6512 6774
Size: 197 926 621 448 1544 1055 901 262
Cuts at: 6774 820
Size: 1553
Fragments arranged by size:
1553 1544 1055 926 901 621 448 262
197
SnaBI TAC'GTA
Cuts at: 493 5298 493
Size: 4805 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7507
SphI G_CATG'C
Cuts at: 1132 2083 3923 4865 1132
Size: 951 1840 942 3774
Fragments arranged by size:
3774 1840 951 942
SspI AAT'ATT
Cuts at: 5 52 3754 5431 5
Size: 47 3702 1677 2081
Fragments arranged by size:
3702 2081 1677 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7507
StyI C'CwwG_G
Cuts at: 513 1065 2004 2340 4371 4671 4946 513
Size: 552 939 336 2031 300 275 3074
Fragments arranged by size:
3074 2031 939 552 336 300 275
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2438 4068 5534 5551 5704 5889 6334
Size: 1255 858 1630 1466 17 153 185 445
Cuts at: 6334 325
Size: 1498
Fragments arranged by size:
1630 1498 1466 1255 858 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2199 3623 4060
Size: 274 80 33 51 1443 222 1424 437
Cuts at: 4060 5753 7465 96
Size: 1693 1712 138
Fragments arranged by size:
1712 1693 1443 1424 437 274 222 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7467 7499 7467
Size: 32 7475
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7507
XbaI T'CTAG_A
Cuts at: 4843 4843
Size: 7507
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2443 2483 4004 2443
Size: 40 1521 5946
Fragments arranged by size:
5946 1521 40
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7507
XmaI C'CCGG_G
Cuts at: 1870 2166 4832 1870
Size: 296 2666 4545
Fragments arranged by size:
4545 2666 296
XmnI GAAnn'nnTTC
Cuts at: 2403 5636 2403
Size: 3233 4274
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5315 276
Size: 53 83 186 936 3781 2468
Fragments arranged by size:
3781 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PstI
PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI DraIII EcoICRI FseI FspAI KasI
NarI NruI NsiI PacI PfoI PspOMI PspXI PsrI
PvuII RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI