Restriction map for
pFN21AB3089
(Circular) (Six-base) MAPSORT of: pFN21AB3089.seq Check: 3802 from: 1 to: 6130
pFN21AB3089 6130 bp
With 182 enzymes: SgfI *
February 27, 2009 14:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3388 3388
Size: 6130
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2628 3940 278
Size: 53 83 186 936 1092 1312 2468
Fragments arranged by size:
2468 1312 1092 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2622 3473 1937
Size: 685 851 4594
Fragments arranged by size:
4594 851 685
Acc65I G'GTAC_C
Cuts at: 3451 3451
Size: 6130
AclI AA'CG_TT
Cuts at: 1563 4257 4630 1563
Size: 2694 373 3063
Fragments arranged by size:
3063 2694 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2163 3014 3168 4193 5577 5845 1632
Size: 531 851 154 1025 1384 268 1917
Fragments arranged by size:
1917 1384 1025 851 531 268 154
AfeI AGC'GCT
Cuts at: 2813 2813
Size: 6130
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5933
AflIII A'CryG_T
Cuts at: 1203 5029 6006 1203
Size: 3826 977 1327
Fragments arranged by size:
3826 1327 977
AgeI A'CCGG_T
Cuts at: 4961 4961
Size: 6130
AhdI GACnn_n'nnGTC
Cuts at: 2344 4859 2344
Size: 2515 3615
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6130
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2036 1184
Size: 852 5278
AlwNI CAG_nnn'CTG
Cuts at: 2056 3873 5445 2056
Size: 1817 1572 2741
Fragments arranged by size:
2741 1817 1572
ApaLI G'TGCA_C
Cuts at: 4187 5343 4187
Size: 1156 4974
ApoI r'AATT_y
Cuts at: 1493 2123 3439 3665 3817 5926 1493
Size: 630 1316 226 152 2109 1697
Fragments arranged by size:
2109 1697 1316 630 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4684 160
Size: 872 3652 1606
Fragments arranged by size:
3652 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6130
AvaI C'yCGr_G
Cuts at: 1870 1943 2587 3455 1870
Size: 73 644 868 4545
Fragments arranged by size:
4545 868 644 73
BamHI G'GATC_C
Cuts at: 3460 3827 3460
Size: 367 5763
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2278 3107 3376 3451 4906
Size: 325 217 627 491 829 269 75 1455
Cuts at: 4906 618
Size: 1842
Fragments arranged by size:
1842 1455 829 627 491 325 269 217
75
BanII G_rGCy'C
Cuts at: 1391 2356 2491 2907 3449 1391
Size: 965 135 416 542 4072
Fragments arranged by size:
4072 965 542 416 135
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2151 3002 3016 928
Size: 971 252 851 14 4042
Fragments arranged by size:
4042 971 851 252 14
BbvCI CC'TCA_GC
Cuts at: 2154 2154
Size: 6130
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4321 4355 1903
Size: 34 2384 34 3678
Fragments arranged by size:
3678 2384 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2468 4022 5232 2468
Size: 1554 1210 3366
Fragments arranged by size:
3366 1554 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6130
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4741 136
Size: 107 122 71 631 605 3069 1525
Fragments arranged by size:
3069 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6125 6125
Size: 6130
BlpI GC'TnA_GC
Cuts at: 3547 3547
Size: 6130
Bme1580I G_kGCm'C
Cuts at: 1790 4191 5347 1790
Size: 2401 1156 2573
Fragments arranged by size:
2573 2401 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6130
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2363 3861 4819
Size: 416 53 83 399 24 946 1498 958
Cuts at: 4819 442
Size: 1753
Fragments arranged by size:
1753 1498 958 946 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6130
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3227 3227
Size: 6130
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2033 4790 1239
Size: 794 2757 2579
Fragments arranged by size:
2757 2579 794
Bpu10I CC'TnA_GC
Cuts at: 2154 2355 2154
Size: 201 5929
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2932 4255 5120 5418 2932
Size: 1323 865 298 3644
Fragments arranged by size:
3644 1323 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4793 882
Size: 617 3294 2219
Fragments arranged by size:
3294 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3921 493
Size: 711 2717 2702
Fragments arranged by size:
2717 2702 711
BsaBI GATnn'nnATC
Cuts at: 6124 6124
Size: 6130
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2625 3937 4319 4954
Size: 53 83 186 936 1092 1312 382 635
Cuts at: 4954 275
Size: 1451
Fragments arranged by size:
1451 1312 1092 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4563 4961 5235 5382 5793 4563
Size: 398 274 147 411 4900
Fragments arranged by size:
4900 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2423 2453 2798 2828 3338
Size: 30 165 30 1020 30 345 30 510
Cuts at: 3338 3368 1178
Size: 30 3940
Fragments arranged by size:
3940 1020 510 345 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2447 2450 1186
Size: 1261 3 4866
Fragments arranged by size:
4866 1261 3
BseYI C'CCAG_C
Cuts at: 5333 5829 5333
Size: 496 5634
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2896 1163
Size: 573 1160 4397
Fragments arranged by size:
4397 1160 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3580 4341 5369 1540
Size: 275 186 1579 761 1028 2301
Fragments arranged by size:
2301 1579 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2804 4191 4276 5347 2804
Size: 1387 85 1071 3587
Fragments arranged by size:
3587 1387 1071 85
BsmI GAATG_Cn'
Cuts at: 3641 3734 3641
Size: 93 6037
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2356 2491 2804 2907 3449 4191 4276
Size: 399 566 135 313 103 542 742 85
Cuts at: 4276 5347 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 566 542 399 313 135
103 85
BspEI T'CCGG_A
Cuts at: 5793 5793
Size: 6130
BspHI T'CATG_A
Cuts at: 4019 5749 4019
Size: 1730 4400
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3388 3485 844
Size: 2544 97 3489
Fragments arranged by size:
3489 2544 97
BsrBI CCG'CTC
Cuts at: 1394 1823 4017 1394
Size: 429 2194 3507
Fragments arranged by size:
3507 2194 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2502 4625 4799 65
Size: 1183 1254 2123 174 1396
Fragments arranged by size:
2123 1396 1254 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4774 4961 1700
Size: 110 2964 187 2869
Fragments arranged by size:
2964 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2301 96
Size: 2205 3925
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6130
BssSI C'ACGA_G
Cuts at: 2024 2807 4190 5202 2024
Size: 783 1383 1012 2952
Fragments arranged by size:
2952 1383 1012 783
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6130
BstBI TT'CG_AA
Cuts at: 4934 4934
Size: 6130
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6130
BstXI CCAn_nnnn'nTGG
Cuts at: 2395 2498 2395
Size: 103 6027
BstYI r'GATC_y
Cuts at: 1972 3460 3827 4212 4229 5681 5767 5779 6125
Size: 1488 367 385 17 1452 86 12 346
Cuts at: 6125 1972
Size: 1977
Fragments arranged by size:
1977 1488 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2132 2132
Size: 6130
BtgI C'CryG_G
Cuts at: 513 1065 2004 2068 2193 2242 2376 3111 513
Size: 552 939 64 125 49 134 735 3532
Fragments arranged by size:
3532 939 735 552 134 125 64 49
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5391
Fragments arranged by size:
5391 683 56
BtsI GCAGTG_nn'
Cuts at: 3646 4440 4460 3646
Size: 794 20 5316
Fragments arranged by size:
5316 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6130
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2271 2306 529
Size: 35 1707 35 4353
Fragments arranged by size:
4353 1707 35 35
DraI TTT'AAA
Cuts at: 3434 3434
Size: 6130
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2324 5137 809
Size: 507 1008 2813 1802
Fragments arranged by size:
2813 1802 1008 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2037 2079 2292
Size: 54 1425 50 275 18 207 42 213
Cuts at: 2292 3114 3577 4466 6095 8
Size: 822 463 889 1629 43
Fragments arranged by size:
1629 1425 889 822 463 275 213 207
54 50 43 42 18
EagI C'GGCC_G
Cuts at: 1537 1812 3577 1537
Size: 275 1765 4090
Fragments arranged by size:
4090 1765 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2092 2831 3227 4060 1353
Size: 739 739 396 833 3423
Fragments arranged by size:
3423 833 739 739 396
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4717 5091 5237 387
Size: 1064 138 3128 374 146 1280
Fragments arranged by size:
3128 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2033 2163 3014 3168 4193 4790 5577
Size: 393 401 130 851 154 1025 597 787
Cuts at: 5577 5845 1239
Size: 268 1524
Fragments arranged by size:
1524 1025 851 787 597 401 393 268
154 130
EcoO109I rG'GnC_Cy
Cuts at: 2136 2649 2136
Size: 513 5617
EcoRI G'AATT_C
Cuts at: 3439 3817 3439
Size: 378 5752
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2908 3349 2908
Size: 441 5689
FspI TGC'GCA
Cuts at: 4636 4636
Size: 6130
HaeII r_GCGC'y
Cuts at: 1678 2815 5277 1678
Size: 1137 2462 2531
Fragments arranged by size:
2531 2462 1137
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2091 2123 2330 2362 2733
Size: 32 135 32 519 32 207 32 371
Cuts at: 2733 2765 2989 3021 3338 3370 4771 4803 4845
Size: 32 224 32 317 32 1401 32 42
Cuts at: 4845 4877 1373
Size: 32 2626
Fragments arranged by size:
2626 1401 519 371 317 224 207 135
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2533 2695 3474 3720 669
Size: 988 281 595 162 779 246 3079
Fragments arranged by size:
3079 988 779 595 281 246 162
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6130
HpaI GTT'AAC
Cuts at: 3720 3720
Size: 6130
KpnI G_GTAC'C
Cuts at: 3455 3455
Size: 6130
MfeI C'AATT_G
Cuts at: 3729 3729
Size: 6130
MluI A'CGCG_T
Cuts at: 6006 6006
Size: 6130
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5244 5428 1305
Size: 3939 184 2007
Fragments arranged by size:
3939 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4308
Fragments arranged by size:
4308 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2333 4088 4447 4606 518
Size: 648 1167 1755 359 159 2042
Fragments arranged by size:
2042 1755 1167 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2070 2153 2378 2558 2931 3546 4223 5371
Size: 464 83 225 180 373 615 677 1148
Cuts at: 5371 5616 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 615 464 373 245 225
180 83
NcoI C'CATG_G
Cuts at: 513 1065 2004 2242 513
Size: 552 939 238 4401
Fragments arranged by size:
4401 939 552 238
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6130
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6130
NotI GC'GGCC_GC
Cuts at: 3577 3577
Size: 6130
NspI r_CATG'y
Cuts at: 1132 1229 2693 3488 5033 1132
Size: 97 1464 795 1545 2229
Fragments arranged by size:
2229 1545 1464 795 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6130
PciI A'CATG_T
Cuts at: 5029 5029
Size: 6130
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2470 1108
Size: 1362 4768
PfoI T'CCnGG_A
Cuts at: 2176 2176
Size: 6130
PmeI GTTT'AAAC
Cuts at: 3434 3434
Size: 6130
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4199 4231 5712 5744 4199
Size: 32 1481 32 4585
Fragments arranged by size:
4585 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2136 2649 2136
Size: 513 5617
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6130
PsiI TTA'TAA
Cuts at: 3700 3700
Size: 6130
PstI C_TGCA'G
Cuts at: 830 3482 830
Size: 2652 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6130
PvuII CAG'CTG
Cuts at: 2153 2558 2153
Size: 405 5725
SacII CC_GC'GG
Cuts at: 2071 2379 2071
Size: 308 5822
SalI G'TCGA_C
Cuts at: 1936 3472 1936
Size: 1536 4594
SanDI GG'GwC_CC
Cuts at: 2649 2649
Size: 6130
SbfI CC_TGCA'GG
Cuts at: 3482 3482
Size: 6130
ScaI AGT'ACT
Cuts at: 4378 6090 4378
Size: 1712 4418
SfcI C'TryA_G
Cuts at: 826 1045 2869 3478 4613 5294 5485 826
Size: 219 1824 609 1135 681 191 1471
Fragments arranged by size:
1824 1471 1135 681 609 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6130
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6130
SmaI CCC'GGG
Cuts at: 1872 3457 1872
Size: 1585 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2911 4234 5135 5397 820
Size: 197 926 968 1323 901 262 1553
Fragments arranged by size:
1553 1323 968 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3921 493
Size: 3428 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6130
SphI G_CATG'C
Cuts at: 1132 3488 1132
Size: 2356 3774
SspI AAT'ATT
Cuts at: 5 52 4054 5
Size: 47 4002 2081
Fragments arranged by size:
4002 2081 47
StuI AGG'CCT
Cuts at: 1517 2867 3086 1517
Size: 1350 219 4561
Fragments arranged by size:
4561 1350 219
StyI C'CwwG_G
Cuts at: 513 1065 2004 2242 2482 2883 3569 513
Size: 552 939 238 240 401 686 3074
Fragments arranged by size:
3074 939 686 552 401 240 238
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2266 4157 4174 4327 4512 4957 325
Size: 1255 686 1891 17 153 185 445 1498
Fragments arranged by size:
1891 1498 1255 686 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2301 4376 6088
Size: 274 80 33 51 1443 324 2075 1712
Cuts at: 6088 96
Size: 138
Fragments arranged by size:
2075 1712 1443 324 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2391 2423 2798 2830 3049 3081 6090 6122 2391
Size: 32 375 32 219 32 3009 32 2399
Fragments arranged by size:
3009 2399 375 219 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2459 1316
Size: 1143 4987
XbaI T'CTAG_A
Cuts at: 3466 3466
Size: 6130
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2329 2681 2329
Size: 352 5778
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6130
XmaI C'CCGG_G
Cuts at: 1870 3455 1870
Size: 1585 4545
XmnI GAAnn'nnTTC
Cuts at: 4259 4259
Size: 6130
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2626 3938 276
Size: 53 83 186 936 1092 1312 2468
Fragments arranged by size:
2468 1312 1092 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacII
SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI
BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI
PspOMI PspXI PsrI RsrII SacI SapI SexAI SfiI
SfoI SrfI SwaI